1. Myogenic Differentiation of Mouse Embryonic Stem Cells That Lack a FunctionalPax7Gene
- Author
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Wladyslawa Streminska, Barbara Swierczek, Anita Helinska, Joanna Bem, Maria A. Ciemerych, Iwona Grabowska, Marta Koblowska, Anna Fogtman, Areta M. Czerwinska, Roksana Iwanicka-Nowicka, and Karolina Archacka
- Subjects
Male ,0301 basic medicine ,KOSR ,Homeobox protein NANOG ,Cellular differentiation ,Embryoid body ,Biology ,Muscle Development ,Mice ,03 medical and health sciences ,Original Research Reports ,Animals ,Induced pluripotent stem cell ,Mice, Knockout ,Genetics ,Gene Expression Regulation, Developmental ,PAX7 Transcription Factor ,Cell Differentiation ,Mouse Embryonic Stem Cells ,Cell Biology ,Hematology ,musculoskeletal system ,Cell biology ,Mice, Inbred C57BL ,MicroRNAs ,030104 developmental biology ,Myogenic regulatory factors ,Female ,Stem cell ,tissues ,Developmental Biology ,Adult stem cell - Abstract
The transcription factor Pax7 plays a key role during embryonic myogenesis and sustains the proper function of satellite cells, which serve as adult skeletal muscle stem cells. Overexpression of Pax7 has been shown to promote the myogenic differentiation of pluripotent stem cells. However, the effects of the absence of functional Pax7 in differentiating embryonic stem cells (ESCs) have not yet been directly tested. Herein, we studied mouse stem cells that lacked a functional Pax7 gene and characterized the differentiation of these stem cells under conditions that promoted the derivation of myoblasts in vitro. We analyzed the expression of myogenic factors, such as myogenic regulatory factors and muscle-specific microRNAs, in wild-type and mutant cells. Finally, we compared the transcriptome of both types of cells and did not find substantial differences in the expression of genes related to the regulation of myogenesis. As a result, we showed that the absence of functional Pax7 does not prevent the in vitro myogenic differentiation of ESCs.
- Published
- 2016
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