1. Antigen-specific human T-cell factors: T-cell helper and suppressor factors, immunochemical aspects.
- Author
-
Heijnen CJ, UytdeHaag F, Pot KH, Bentwich Z, and Ballieux RE
- Subjects
- Animals, Antibody Affinity, Chemical Phenomena, Chemistry, Glycoproteins, Histocompatibility Antigens Class II immunology, Humans, Interleukin-1 metabolism, Lymphokines metabolism, Mice, Rabbits, Suppressor Factors, Immunologic, beta 2-Microglobulin immunology, Epitopes analysis, Interleukin-1 analysis, Lymphokines analysis, T-Lymphocytes immunology
- Abstract
Some immunochemical characteristics of a T-cell-derived ovalbumin (OA) antigen-specific helper (ThF120-OA) and a corresponding OA-specific suppressor effector factor (TsF120-OA) are described. These immunoregulatory molecules are retained by columns containing either the insolubilized antigen OA or the lectin ConA, or antibodies specific for the VH-region of immunoglobulins, for the beta 2-microglobulin or for Ia-framework determinants. Neither the helper factor nor the suppressor factor showed affinity for antisera specific for immunoglobulin isotypes. In search of a constant-region-like structure in factor molecules, antisera were raised in rabbits using ThF120-OA and TsF120-OA. The results show that antisera raised against ThF120-OA recognize one or more determinants of different antigenic specificities common to human ThF. This common structure is not species-specific since it is also recognized by rabbit antisera to mouse helper factor. It is absent, however, on human TsF120-OA. In the same way, human TsF120-OA was shown to interact with rabbit antisera to human (or murine) suppressor factors of different antigenic specificities, but not with anti-ThF.
- Published
- 1982