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High-rise building areas (HRBs) provide significant social and environmental services and play a crucial role in modern urbanization. The large-scale and long-term spatial distribution of HRBs is of great interest to many fields, such as urban planning and local climate analysis. While previous studies have confirmed the value of Sentinel-2 images in extracting HRBs and their changes, current work is limited to relatively local areas and short-term analysis. One reason is due to the fact that the earliest Sentinel-2 image can only date back to 2015. To address this research gap, this paper proposes an efficient procedure to intelligently extract HRBs and their changes from multitemporal Landsat-7 and Sentinel-2 images, using a specifically designed fully convolutional network. To validate the proposed method, we selected four typical cities in China, namely, Beijing, Shanghai, Guangzhou, and Zhengzhou, as study areas. We utilized Landsat-7 images acquired in 2000 and 2010, along with Sentinel-2 images acquired in 2020, as experimental data. We extracted and analyzed three periods of HRBs and their changes in the four cities, along with urban rail terminal data and gross domestic product (GDP) data in the same period. The results show that the proposed method can efficiently extract HRBs and their changes in the four cities over the past 20 years, with an overall accuracy of more than 90%. HRBs changes are primarily driven by urban planning policies and geographical factors. There is a strong positive correlation between the increase in HRBs and the increase in rail terminals, both in terms of quantity and spatial distribution. Additionally, there is a positive correlation between HRBs increase and GDP increase in terms of quantity, but the trend varies in different cities due to their diverse developing modes. Overall, the results indicate that the proposed method can be a potential operational tool to extract large-scale and long-term HRBs and their changes in China. [ABSTRACT FROM AUTHOR]

Since 2018, the outbreak and prevalence of the African swine fever virus (ASFV) in China have caused huge economic losses. Less virulent ASFVs emerged in 2020, which led to difficulties and challenges for early diagnosis and control of African swine fever (ASF) in China. An effective method of monitoring ASFV antibodies and specific antibodies against ASFV to promote the development of prevention techniques are urgently needed. In the present study, ASFV p17 was successfully expressed in CHO cells using a suspension culture system. An indirect enzyme-linked immunosorbent assay (ELISA) based on purified p17 was established and optimized. The monoclonal antibody (mAb) against p17 recognized a conservative linear epitope (3TETSPLLSH11) and exhibited specific reactivity, which was conducive to the identification of recombinant porcine reproductive and respiratory syndrome virus (PRRSV) expressing p17. The ELISA method efficiently detected clinical ASFV infection and effectively monitored the antibody levels in vivo after recombinant PRRSV live vector virus expressing p17 vaccination. Overall, the determination of the conserved linear epitope of p17 would contribute to the in-depth exploration of the biological function of the ASFV antigen protein. The indirect ELISA method and mAb against ASFV p17 revealed efficient detection and promising application prospects, making them ideal for epidemiological surveillance and vaccine research on ASF. [ABSTRACT FROM AUTHOR]

Porcine reproductive and respiratory syndrome (PRRS) is an important viral disease, causing significant economic losses to the swine industry worldwide. Atypical cases caused by highly pathogenic PRRS virus (HP-PRRSV) emerged in 2006 in China. The vaccine strain HuN4-F112 has been developed from the wild-type HP-PRRSV HuN4 through repeated passages on MARC-145 cells. However, the mechanisms of attenuation have yet to be defined. Previous studies have shown that the vaccine strain HuN4-F112 could not effectively replicate in porcine alveolar macrophages (PAMs). In the present study, a series of chimeric and mutant PRRSVs were constructed to investigate regions associated with the virus attenuation. Firstly, the corresponding genome regions (ORF1a, ORF1b and ORFs 2-7) were exchanged between two infectious clones of HuN4 and HuN4-F112, and then the influence of small regions in ORF1a and ORF2-7 was evaluated, then influence of specific amino acids on NSP2 was tested. NSP2 was determined to be the key gene that regulated infection efficiency on PAMs, and amino acids at 893 and 979 of NSP2 were the key amino acids. The results of in vivo study indicated that NSP2 was not only important for infection efficiency in vitro, but also influenced the virulence, which was indicated by the results of survival rate, temperature, viremia, lung score and tissue score. [ABSTRACT FROM AUTHOR]

Simple Summary: African swine fever (ASF) is the most serious animal disease that endangers the pig industry in China, causing the heaviest economic loss so far. Effective prevention and control of ASF is necessary for China's pig industry, and also the focus and hotspot of global swine industry disease prevention and control research. In view of this, rapid, specific and sensitive diagnosis of ASF is of great significance. In this study, ASF virus (ASFV) E248R gene was selected to be the target for establishing a real-time PCR method which did not cross-react with other porcine viruses that could cause similar symptoms. The detection methods can be used for the efficient detection of ASFV infection and the recombinant live-vectored virus-expressing antigen protein of ASFV. ASF has caused huge economic losses to China's swine industry. As clinical symptoms of ASF were difficult to distinguish from classical swine fever and porcine reproductive and respiratory syndrome (PRRS), rapid and effective differential diagnosis of ASFV seems very important to control the spread of the disease. In this study, the ASFV E248R gene was selected to be the target for establishing a real-time PCR method. TaqMan real-time PCR for the detection of ASFV E248R gene did not cross-react with other porcine viruses that could cause similar symptoms. The results of the repeatability test showed that the coefficients of variation between and within groups were lower than 1.977%. This method can be used for the rapid detection and early diagnosis of ASF. Meanwhile, the recombinant PRRS virus (PRRSV)-expressing E248R gene of ASFV was constructed and rescued by using the reverse genetic platform of live-attenuated PRRSV vaccine. The ASFV E248R gene can be detected by using this real-time PCR detection method, confirming that the ASFV E248R gene could be stably amplified in PRRSV genome at least 20 cell passages. The detection methods can be used for the efficient detection of the ASFV infection and recombinant PRRSV live vector virus-expressing ASFV antigen protein. [ABSTRACT FROM AUTHOR]

Wetlands have been degrading and reducing under the influences of human activity and climate change. Landsat long-term observations can help us better track the changing evidences of wetland habitats that would be valuable for guiding the restoration and conservation of wetland. In this study, we demonstrated the results of tracking the changing evidence of wetland habitats using Landsat observations from 1984 to 2017 through the case study of Baiyangdian wetland in China. We extracted the open water and classified the wetland habitats using collected 190 scenes from Landsat observations. As a result, we found that the yearly variations of wetland present phasic changes in three phases: 1988–1998, 1999–2011 and 2013–2017. The landscape of wetland habitats presented during 1989–1999 mostly show us the natural spatial pattern with less human disturbance traces compared to that during 2013–2017. The water environment, moreover, changed for the better after the 2010s, which indicated the encouraging effects of the environmental restoration project implemented from the year 2010. The current landscapes of wetland habitats, however, present lots of linear belts that are blocking the water cycles and ecological channels of aquatic plants and animals in the wetland. The areas in the northwestern wing and around the northeastern edge of the wetland are changing to be drier due to cropping activities and are at risk of wetland loss. These historical changing evidences could be a guideline for planning and designing restoration for the wetland. [ABSTRACT FROM AUTHOR]