1. Selective Expansion of NKG2C+ Adaptive NK Cells Using K562 Cells Expressing HLA-E.
- Author
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Phan, Minh-Trang Thi, Kim, Jinho, Koh, Seung Kwon, Lim, Yuree, Yu, Hongbi, Lee, Mijeong, Lee, Jong-Min, Kang, Eun-Suk, Kim, Hyun-Young, Kim, Sang-Ki, Hwang, Ilwoong, and Cho, Duck
- Subjects
KILLER cells ,LYMPHOBLASTIC leukemia ,HUMAN cytomegalovirus ,CANCER cells ,ACUTE leukemia - Abstract
Adaptive natural killer (NK) cells expressing self-specific inhibitory killer-cell immunoglobulin-like receptors (KIRs) can be expanded in vivo in response to human cytomegalovirus (HCMV) infection. Developing a method to preferentially expand this subset is essential for effective targeting of allogeneic cancer cells. A previous study developed an in vitro method to generate single KIR+ NK cells for enhanced targeting of the primary acute lymphoblastic leukemia cells; however, the expansion rate was quite low. Here, we present an effective expansion method using genetically modified K562-HLA-E feeder cells for long-term proliferation of adaptive NK cells displaying highly differentiated phenotype and comparable cytotoxicity, CD107a, and interferon-γ (IFN-γ) production. More importantly, our expansion method achieved more than a 10,000-fold expansion of adaptive NK cells after 6 weeks of culture, providing a high yield of alloreactive NK cells for cell therapy against cancer. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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