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The presence of certain proteins in biofluids such as synovial fluid, blood plasma, and saliva gives these fluids non-Newtonian viscoelastic properties. The amount of these protein macromolecules in biofluids is an important biomarker for the diagnosis of various health conditions, including Alzheimer's disease, cardiovascular disorders, and joint quality. However, existing technologies for measuring the behavior of macromolecules in biofluids have limitations, such as long turnaround times, complex protocols, and insufficient sensitivity. To address these issues, we propose non-contact, optical Brillouin and Raman spectroscopy to assess the viscoelasticity and chemistry of non-Newtonian solutions, respectively, at different temperatures in several minutes. In this work, bovine and human serum albumin solution-based biopolymers were studied to obtain both their collective dynamics and molecular chemical evolution across heat-driven phase transitions at various protein concentrations. The observed phase transitions at elevated temperatures could be fully delayed in heated biopolymers by appropriately raising the level of protein concentration. The non-contact optical monitoring of viscoelastic and chemical property evolution could represent novel potential mechano-chemical biomarkers for disease diagnosis and subsequent treatment applications, including hyperthermia. [ABSTRACT FROM AUTHOR]

The complex physical transformations of polymers upon external thermodynamic changes are related to the molecular length of the polymer and its associated multifaceted energetic balance. The understanding of subtle transitions or multistep phase transformation requires real-time phenomenological studies using a multi-technique approach that covers several length-scales and chemical states. A combination of X-ray scattering techniques with Raman spectroscopy and Differential Scanning Calorimetry was conducted to correlate the structural changes from the conformational chain to the polymer crystal and mesoscale organization. Current research applications and the experimental combination of Raman spectroscopy with simultaneous SAXS/WAXS measurements coupled to a DSC is discussed. In particular, we show that in order to obtain the maximum benefit from simultaneously obtained high-quality data sets from different techniques, one should look beyond traditional analysis techniques and instead apply multivariate analysis. Data mining strategies can be applied to develop methods to control polymer processing in an industrial context. Crystallization studies of a PVDF blend with a fluoroelastomer, known to feature complex phase transitions, were used to validate the combined approach and further analyzed by MVA. [ABSTRACT FROM AUTHOR]

Deterministic or statistical inversion schemes to retrieve ocean color from space often use a simplified water reflectance model that may introduce unrealistic constraints on the solution, a disadvantage compared with standard, two-step algorithms that make minimal assumptions about the water signal. In view of this, the semi-analytical models of Morel and Maritorena (2001), MM01, and Park and Ruddick (2005), PR05, used in the spectral matching POLYMER algorithm (Steinmetz et al., 2011), are examined in terms of their ability to restitute properly, i.e., with sufficient accuracy, water reflectance. The approach is to infer water reflectance at MODIS wavelengths, as in POLYMER, from theoretical simulations (using Hydrolight with fluorescence and Raman scattering) and, separately, from measurements (AERONET-OC network). A wide range of Case 1 and Case 2 waters, except extremely turbid waters, are included in the simulations and sampled in the measurements. The reflectance model parameters that give the best fit with the simulated data or the measurements are determined. The accuracy of the reconstructed water reflectance and its effect on the retrieval of inherent optical properties (IOPs) is quantified. The impact of cloud and aerosol transmittance, fixed to unity in the POLYMER scheme, on model performance is also evaluated. Agreement is generally good between model results and Hydrolight simulations or AERONET-OC values, even in optically complex waters, with discrepancies much smaller than typical atmospheric correction errors. Significant differences exist in some cases, but having a more intricate model (i.e., using more parameters) makes convergence more difficult. The trade-off is between efficiency/robustness and accuracy. Notable errors are obtained when using the model estimates to retrieve IOPs. Importantly, the model parameters that best fit the input data, in particular chlorophyll-a concentration, do not represent adequately actual values. The reconstructed water reflectance should be used in bio-optical algorithms. While neglecting cloud and aerosol transmittances degrades the accuracy of the reconstructed water reflectance and the retrieved IOPs, it negligibly affects water reflectance ratios and, therefore, any variable derived from such ratios. [ABSTRACT FROM AUTHOR]

Core-shell microspheres have been applied in various research areas and, in particular, they are used in the generation of photonic nanojets with suitable design for photonic applications. The photonic nanojet is a narrow and focused high-intensity light beam emitting from the shadow-side of microspheres with tunable effective length, thus enabling its applications in biosensing technology. In this paper, we numerically studied the photonic nanojets brought about from biocompatible hydrogel core-shell microspheres with different optical properties. It was found that the presence of the shell layer can significantly affect the characteristics of the photonic nanojets, such as the focal distance, intensity, effective length, and focal size. Generally speaking, the larger the core-shell microspheres, the longer the focal distance, the stronger the intensity, the longer the effective length, and the larger the focal size of the generated photonic nanojets are. The numerical simulations of the photonic nanojets from the biocompatible core-shell microspheres on a Klarite substrate, which is a classical surface-enhancing Raman scattering substrate, showed that the Raman signals in the case of adding the core-shell microspheres in the system can be further enhanced 23 times in water and 108 times in air as compared in the case in which no core-shell microspheres are present. Our study of using tunable photonic nanojets produced from the biocompatible hydrogel core-shell microspheres shows potential in future biosensing applications. [ABSTRACT FROM AUTHOR]