1. Production of Salvianic Acid A from l-DOPA via Biocatalytic Cascade Reactions.
- Author
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Hu, Ke Shun, Chen, Chong Le, Ding, Huan Ru, Wang, Tian Yu, Zhu, Qin, Zhou, Yi Chen, Chen, Jia Min, Mei, Jia Qi, Hu, Sheng, Huang, Jun, Zhao, Wei Rui, and Mei, Le He
- Subjects
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SALVIA miltiorrhiza , *DOPA , *ESCHERICHIA coli , *PEDIOCOCCUS acidilactici , *BIOACTIVE compounds , *ACIDS - Abstract
Salvianic acid A (SAA), as the main bioactive component of the traditional Chinese herb Salvia miltiorrhiza, has important application value in the treatment of cardiovascular diseases. In this study, a two-step bioprocess for the preparation of SAA from l-DOPA was developed. In the first step, l-DOPA was transformed to 3,4-dihydroxyphenylalanine (DHPPA) using engineered Escherichia coli cells expressing membrane-bound L-amino acid deaminase from Proteus vulgaris. After that, the unpurified DHPPA was directly converted into SAA by permeabilized recombinant E. coli cells co-expressing d-lactate dehydrogenase from Pediococcus acidilactici and formate dehydrogenase from Mycobacterium vaccae N10. Under optimized conditions, 48.3 mM of SAA could be prepared from 50 mM of l-DOPA, with a yield of 96.6%. Therefore, the bioprocess developed here was not only environmentally friendly, but also exhibited excellent production efficiency and, thus, is promising for industrial SAA production. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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