1. Love-Wave Sensors Combined with Microfluidics for Fast Detection of Biological Warfare Agents.
- Author
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Matatagui, Daniel, Fontecha, José Luis, Fernández, María Jesús, Gràcia, Isabel, Cané, Carles, Santos, José Pedro, and Horrillo, María Carmen
- Subjects
- *
BIOLOGICAL warfare research , *MICROFLUIDIC devices , *MICROPROCESSORS , *MONOCLONAL antibodies , *IMMUNOGLOBULIN G - Abstract
The following paper examines a time-efficient method for detecting biological warfare agents (BWAs). The method is based on a system of a Love-wave immunosensor combined with a microfluidic chip which detects BWA samples in a dynamic mode. In this way a continuous flow-through of the sample is created, promoting the reaction between antigen and antibody and allowing a fast detection of the BWAs. In order to prove this method, static and dynamic modes have been simulated and different concentrations of BWA simulants have been tested with two immunoreactions: phage M13 has been detected using the mouse monoclonal antibody anti-M13 (AM13), and the rabbit immunoglobulin (Rabbit IgG) has been detected using the polyclonal antibody goat anti-rabbit (GAR). Finally, different concentrations of each BWA simulants have been detected with a fast response time and a desirable level of discrimination among them has been achieved. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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