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The rapid melting of glaciers has led to severe glacial-hydrological hazards in the Himalayas. An extreme example occurred on 7 February 2021, when a catastrophic mass flow descended from the Ronti glacier at Chamoli, Indian Himalaya, causing widespread devastation, with more than 200 people killed or missing, as well as severe damage to four hydropower projects. To disclose what happened to the Ronti glacier over the past several decades, here, we focused on glacier changes in the Dhauliganga catchment in Uttarakhand, India, over the past two decades. Another five glaciers in the catchment were also studied to map the regional detailed glacier changes. Our achievements are summarized as follows. (1) Based on Landsat images, we constructed two glacier inventories for the catchment in 2001 and 2020. We mapped nearly 413 debris-free glaciers in the catchment between 2001 and 2020 and analyzed the glacier area change at basin and altitude levels. (2) Debris-free glacier area decreased from 477.48 ± 35.23 km2 in 2001 to 418.52 ± 36.18 km2 in 2020, with a reduction of 58.95 km2 or 12.35% over the past two decades. (3) The geodetic mass balance was −0.27± 0.10 m w.e.a−1, with a glacier mass change of −0.12 Gt. a−1 from 2000 to 2013. Based on the surface elevation difference between the Ice, Cloud, and land Elevation Satellite 2 (ICESat-2) footprints (acquired from 2018 to 2021) and the National Aeronautics and Space Administration (NASA) DEM from 2000 to 2021, the average glacier geodetic mass balance was −0.22 ± 0.005 m w.e.a−1, and glacier mass change was −0.10 Gt a−1. (4) Our results were cross verified by available published elevation difference datasets covering multiple temporal periods, where mass balance was by −0.22 ± 0.002 m w.e.a−1 from 1975 to 2000 and −0.28 ± 0.0001 w.e.a−1 from 2000 to 2020. (5) Glacier 1 and Glacier 2, the two largest glaciers in the catchment, experienced a decreasing melt rate from 2000 to 2020, while Glacier 3, Glacier 4, and Glacier 5 demonstrated an increasing melt rate. However, Glacier 6, also known as the collapsed Ronti glacier, had a negative mass balance of −0.04 m w.e.a−1 from 2000 to 2005 and turned positive from 2005 onward with 0.06 m w.e.a−1 from 2005 to 2010, 0.19 m w.e.a−1 from 2010 to 2015, and 0.32 m w.e.a−1 from 2015 to 2020. We postulate that the Ronti glacier collapsed solely because of the significant mass accumulation observed between 3700 to 5500 m a.s.l. Our study helps to understand the collapsed glacier's mass changes over the past two decades and highlights the necessity to monitor mass-gaining glaciers from space to forecast the risks of disasters. [ABSTRACT FROM AUTHOR]

Data-independent acquisition (DIA) based strategies have been explored in recent years for improving quantitative analysis of metabolites. However, the data analysis is challenging for DIA methods as the resulting spectra are highly multiplexed. Thus, the DIA mode requires advanced software analysis to facilitate the data deconvolution process. We proposed a pipeline for quantitative profiling of pharmaceutical drugs and serum metabolites in DIA mode after comparing the results obtained from full-scan, Data-dependent acquisition (DDA) and DIA modes. using open-access software. Pharmaceutical drugs (10) were pooled in healthy human serum and analysed by LC-ESI-QTOF-MS. MS1 full-scan and Data-dependent (MS2) results were used for identification using MS-DIAL software while deconvolution of MS1/MS2 spectra in DIA mode was achieved by using Skyline software. The results of acquisition methods for quantitative analysis validated the remarkable analytical performance of the constructed workflow, proving it to be a sensitive and reproducible pipeline for biological complex fluids. [ABSTRACT FROM AUTHOR]

Staphylotrichum longicolleum FW57 (DSM105789) is a prolific chitinolytic fungus isolated from wood, with a chitinase activity of 0.11 ± 0.01 U/mg. We selected this strain for genome sequencing and annotation, and compiled its growth characteristics on four different chitinous substrates as well as two agro-industrial waste products. We found that the enzymatic mixture secreted by FW57 was not only able to digest pre-treated sugarcane bagasse, but also untreated sugarcane bagasse and maize leaves. The efficiency was comparable to a commercial enzymatic cocktail, highlighting the potential of the S. longicolleum enzyme mixture as an alternative pretreatment method. To further characterize the enzymes, which efficiently digested polymers such as cellulose, hemicellulose, pectin, starch, and lignin, we performed in-depth mass spectrometry-based secretome analysis using tryptic peptides from in-gel and in-solution digestions. Depending on the growth conditions, we were able to detect from 442 to 1092 proteins, which were annotated to identify from 134 to 224 putative carbohydrate-active enzymes (CAZymes) in five different families: glycoside hydrolases, auxiliary activities, carbohydrate esterases, polysaccharide lyases, glycosyl transferases, and proteins containing a carbohydrate-binding module, as well as combinations thereof. The FW57 enzyme mixture could be used to replace commercial enzyme cocktails for the digestion of agro-residual substrates. [ABSTRACT FROM AUTHOR]

The discovery and identification of novel natural products of medicinal importance in the herbal medicine industry becomes a challenge. The complexity of this process can be reduced by dereplication strategies. The current study includes a method based on high-performance liquid chromatography (HPLC), using the evaporative light scattering detector (ELSD) to identify the 12 most common secondary metabolites in plant extracts. Twelve compounds including rutin, taxifolin, quercetin, apigenin, kaempferol, betulinic acid, oleanolic acid, betulin, lupeol, stigmasterol, and β-sitosterol were analyzed simultaneously. The polarity of the compounds varied greatly from highly polar (flavonoids) to non-polar (triterpenes and sterols). This method was also tested for HPLC-DAD and HPLC-ESI-MS/MS analysis. Oleanolic acid and ursolic acid could not be separated in HPLC-ELSD analysis but were differentiated using LC-ESI-MS/MS analysis due to different fragment ions. The regression values (R2 > 0.996) showed good linearity in the range of 50–1000 µg/mL for all compounds. The range of LOD and LOQ values were 7.76–38.30 µg/mL and 23.52–116.06 µg/mL, respectively. %RSD and % trueness values of inter and intraday studies were mostly <10%. This method was applied on 10 species of medicinal plants. The dereplication strategy has the potential to facilitate and shorten the identification process of common secondary metabolites in complex plant extracts. [ABSTRACT FROM AUTHOR]