1. Effect of Dietary Restriction on Gut Microbiota and Brain–Gut Short Neuropeptide F in Mud Crab, Scylla paramamosain.
- Author
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Bao, Chenchang, Yang, Yanan, and Ye, Haihui
- Abstract
Simple Summary: While the gut microbiota and gut–brain sNPF are known to play crucial roles in diet restriction, research on their response to feed deprivation and starvation stress remains limited. This work investigated the alterations in the gut microbiota of juvenile mud crabs under feed deprivation and starvation conditions and identified the location of sNPF in the brain and gut. This suggests a potential interplay between diet restriction, gut microbiota, and gut–brain sNPF. The increased expression levels of sNPF across various levels of feed deprivation further support this notion. This study suggests that sNPF may regulate digestive activities and immunity through interactions with the gut microbiota. These findings provide novel insights into the dynamic interplay between the gut microbiota and sNPF in response to diet restriction, emphasizing their crucial roles in physiological adaptation to starvation stress. Aquatic animals frequently undergo feed deprivation and starvation stress. It is well-known that the gut microbiota and the gut–brain short neuropeptide F (sNPF) play essential roles in diet restriction. Therefore, investigating the responses of the gut microbiota and sNPF can enhance our understanding of physiological adaptations to feed deprivation and starvation stress. In this study, we examined the alterations in the gut microbiota of juvenile mud crabs under feed deprivation and starvation conditions. The results reveal differences in the richness and diversity of gut microbiota among the satisfied, half food, and starvation groups. Moreover, the microbial composition was affected by starvation stress, and more than 30 bacterial taxa exhibited significantly different abundances among the three feeding conditions. These results indicate that the diversity and composition of the gut microbiota are influenced by diet restriction, potentially involving interactions with the gut–brain sNPF. Subsequently, we detected the location of sNPF in the brains and guts of mud crabs through immunofluorescence and investigated the expression profile of sNPF under different feeding conditions. The results suggest that sNPF is located in both the brains and guts of mud crabs and shows increased expression levels among different degrees of diet restriction during a 96 h period. This study suggested a potential role for sNPF in regulating digestive activities and immunity through interactions with the gut microbiota. In conclusion, these findings significantly contribute to our understanding of the dynamic changes in gut microbiota and sNPF, highlighting their interplay in response to diet restriction. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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