1. Bioevaluation of Novel Anti-Biofilm Coatings Based on PVP/Fe3O4 Nanostructures and 2-((4-Ethylphenoxy)methyl)-N-(arylcarbamothioyl)benzamides
- Author
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Gabriel Socol, Diana C Nuță, Carmen Limban, Alexandru Mihai Grumezescu, Alexandra Elena Oprea, Miron Teodor Caproiu, Roxana Trușcă, Alexandru Vasile Missir, Bogdan Ștefan Vasile, Laurențiu Morușciag, Grațiela Pîrcălăbioru, Bianca Gălățeanu, Marieta Costache, Valentina Grumezescu, and Mariana Carmen Chifiriuc
- Subjects
Staphylococcus aureus ,magnetite ,Magnetic Resonance Spectroscopy ,Pyrrolidines ,benzamides ,Pharmaceutical Science ,Article ,Analytical Chemistry ,lcsh:QD241-441 ,core/shell nanostructure ,chemistry.chemical_compound ,lcsh:Organic chemistry ,Drug Discovery ,Fluorescence microscope ,medicine ,Organic chemistry ,Humans ,MTT assay ,anti-biofilm ,Physical and Theoretical Chemistry ,Polyvinylpyrrolidone ,Organic Chemistry ,Biofilm ,Nuclear magnetic resonance spectroscopy ,Anti-Bacterial Agents ,Nanostructures ,chemistry ,Thiourea ,Chemistry (miscellaneous) ,Biofilms ,Isothiocyanate ,thiourea derivatives ,Pseudomonas aeruginosa ,Molecular Medicine ,Magnetic nanoparticles ,Polyvinyls ,Iron Compounds ,Nuclear chemistry ,medicine.drug - Abstract
Novel derivatives were prepared by reaction of aromatic amines with 2-(4-ethylphenoxymethyl)benzoyl isothiocyanate, affording the N-[2-(4-ethylphenoxymethyl) benzoyl]-Nꞌ-(substituted phenyl)thiourea. Structural elucidation of these compounds was performed by IR, NMR spectroscopy and elemental analysis. The new compounds were used in combination with Fe3O4 and polyvinylpyrrolidone (PVP) for the coating of medical surfaces. In our experiments, catheter pieces were coated by Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique. The microbial adherence ability was investigated in 6 multi-well plates by using culture based methods. The obtained surfaces were also assessed for their cytotoxicity with respect to osteoblast cells, by using fluorescence microscopy and MTT assay. The prepared surfaces by advanced laser processing inhibited the adherence and biofilm development ability of Staphylococcus aureus and Pseudomonas aeruginosa tested strains while cytotoxic effects on the 3T3-E1 preosteoblasts embedded in layer shaped alginate hydrogels were not observed. These results suggest that the obtained medical surfaces, based on the novel thiourea derivatives and magnetic nanoparticles with a polymeric shell could represent a promising alternative for the development of new and effective anti-infective strategies.
- Published
- 2014