1. Effect of Black Grape Juice against Heart Damage from Acute Gamma TBI in Rats
- Author
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Liliane de Freitas Bauerman, J.P. Barrio, João Rocha, Thiele Faccim de Brum, Roberta da Silva Jesus, Margareth Linde Athayde, Mariana Piana, Aline Augusti Boligon, Robson Borba de Freitas, Nelson Mendes Alves, Fagner Chagas Rother, Edson R. Andrade, and Bruno Tomazele Rovani
- Subjects
Male ,phenolics ,black grape juice ,Pharmaceutical Science ,Radiation-Protective Agents ,heart ,oxidative damage ,Resveratrol ,Pharmacology ,Placebo ,HPLC-DAD ,High-performance liquid chromatography ,Thiobarbituric Acid Reactive Substances ,Article ,Antioxidants ,Analytical Chemistry ,acute radiation syndrome ,lcsh:QD241-441 ,Lipid peroxidation ,chemistry.chemical_compound ,Blood serum ,lcsh:Organic chemistry ,Phenols ,Lactate dehydrogenase ,Malondialdehyde ,Drug Discovery ,Animals ,lactate dehydrogenase ,flavonoids ,Vitis ,Physical and Theoretical Chemistry ,Rats, Wistar ,Flavonoids ,Chromatography ,L-Lactate Dehydrogenase ,Plant Extracts ,Organic Chemistry ,Total body irradiation ,Rats ,Radiation Injuries, Experimental ,chemistry ,Phytochemical ,Chemistry (miscellaneous) ,Gamma Rays ,Fruit ,Molecular Medicine ,Lipid Peroxidation ,Whole-Body Irradiation - Abstract
The aim of this study was to evaluate the potential positive effect of black grape juice (BGJ) on lipid peroxidation considering Total Body Irradiation (TBI) in Wistar rats. As a potential feasible means of evaluation in situ, blood serum lactate dehydrogenase (LDH) levels were evaluated as a marker for heart damage from acute radiation syndrome (ARS). Twenty rats were divided into four groups, two of them being irradiated by gamma-rays from a Co-60 source. Animals were treated by gavage with 2 mL per day of BGJ or placebo for one week before and 4 days after 6 Gy whole body gamma-irradiation, when they were euthanasiated. LDH on serum and lipid peroxidation on heart tissue were evaluated. High concentration of metabolites from lipid peroxidation in heart, and high LDH level on serum were found only in gamma-irradiated group given placebo, mainly at the first 24 h after radiation. Phytochemical analysis of BGJ was performed by determining total phenolics, flavonoids, and tannins followed by a high-performance liquid chromatography (HPLC/DAD) analysis, which showed resveratrol as the major constituent. Results suggest that BGJ is a good protective candidate compound against heart damage from ARS and its effects suggest its use as a radiomodifier.
- Published
- 2013