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The impact of trace metal elements (TMEs) on plants is one current pollution problem, the severity of which is increasing with industrial development, population growth and inappropriate agricultural practices. The latter can have irreversible effects on ecosystems, including species extinction, trophic chain contamination and altered human health, particularly in the case of consumed plants such as zucchini squash (Cucurbita pepo L.). This study aims to investigate the effects of nickel on various physiological and biochemical parameters of zucchini growth, with a particular focus on how this toxic metal impacts the quality of fruit that is consumed by humans. To achieve this, plants aged 45 days were grown for one month on solid media loaded with different concentrations of Ni (0, 100, 300 and 500 µM). The results showed that exposure of plants to Ni resulted in significantly altered growth and higher accumulation of Ni in the shoots (1314 µg·g−1 DW) than in roots and fruits. Concerning non-enzymatic antioxidants, the results showed that Ni toxicity significantly increased total polyphenols, especially in shoots at 300 µM Ni, while flavonoid content decreased in the roots and shoots in response to Ni treatment. Our results also show that nickel tolerance in C. pepo is ensured by a combination of several mechanisms such as an increase in the content of proline. This species can survive and tolerate, to different degrees, toxic cations at concentrations up to 500 µM but with visible symptoms of toxicity such as chlorosis of the leaves. Indeed, based on thresholds of hyperaccumulation, we can qualify Cucurbita pepo as a hyperaccumulator species of nickel. [ABSTRACT FROM AUTHOR]

Salt marshes play a critical role in supporting water quality, erosion control, flood protection, and carbon sequestration. Threats from climate change and human activities have prompted global restoration initiatives. We analyzed restoration efforts worldwide from 1978 to 2022, using the Web of Science database and SciMAT mapping tool. After a PRISMA screening to identify methodologies, success rates, and key indicators, a total of 62 publications underwent detailed analysis, to increase knowledge on the best practices to employ in future restoration interventions and evaluation of their effectiveness. The research reveals a growing interest in ecosystem dynamics, biodiversity, anthropogenic impacts, and ecosystem services. Assisted interventions emerged as the predominant restoration method, employing 15 indicators across vegetation, sediment, fauna, and water, each one using different metrics for the intervention evaluation based on how good the outcome of the interventions described in the reviewed studies met the desired result. Our analysis suggests that combining natural interventions such as managed realignment with reconnection to tidal waters, along with long-term monitoring of vegetation, fauna, and water indicators such as sedimentation and erosion rates, plant cover and biomass, as well as fauna diversity and density, leads to the most successful outcomes. We provide valuable insights into best practices for future restoration interventions, offering guidance to future practitioners and policymakers based on a comprehensive review of the scientific literature, contributing to the resilience of these vital ecosystems, and ensuring effective restoration actions in the coming years. [ABSTRACT FROM AUTHOR]

Rare earth elements were extensively employed for many years to improve plant growth in farming. However, their effect on plant's behavior relies on their concentration and the plant species. The impact of low doses of lanthanum (La; 1–10 µM) on plant growth, mineral uptake, and the production of secondary metabolites was assessed in two Brassicaceae species (Cakile maritime and Brassica juncea) after 14 days of La exposure. The La accumulation potential was also evaluated. Results showed that both species were able to maintain good dry biomass production under La. C. maritima plants accumulated more La than B. juncea, and a higher accumulation was noticed in the roots (in both plant species). Accordingly to La accumulation in plant tissues, nutrient absorption was affected in C. maritima shoots and roots, whereas no severe effect on nutrient contents was noticed in B. juncea. Phenolic compounds increased in the aerial and underground parts of both species; thus, the accretion was more notorious in shoots of the highest La concentrations. The studied Brassicaceae species showed an ability to survive in a La-contaminated medium. However, according to tolerance index values, C. maritima was found to be more tolerant of La than B. juncea. [ABSTRACT FROM AUTHOR]

Atmospheric CO2 enrichment, which is caused to a large extent by anthropogenic activities, is known to interfere with sediment microbial communities via plant rhizospheres. The present work aimed to evaluate this interaction in Spartina maritima ((Curtis) Fernald.) rhizosediments, aiming to depict the impacts of atmospheric CO2 increase in the biogeochemical processes occurring in the rhizosphere of this pioneer and highly abundant Mediterranean halophyte. For this purpose, mesocosms trials were conducted, exposing salt marsh cores with S. maritima and its sediments to 410 and 700 ppm of CO2 while assessing rhizosediment extracellular enzymatic activities. An evident increase in dehydrogenase activity was observed and directly linked to microbial activity, indicating a priming effect in the rhizosphere community under increased CO2. Phosphatase showed a marked increase in rhizosediments exposed to elevated CO2, denoting a higher requirement of phosphate for maintaining higher biological activity rates. High sulphatase activity suggests a possible S-limitation (microbial or plant) due to elevated CO2, probably due to higher sulphur needs for protein synthesis, thus increasing the need to acquire more labile forms of sulphur. With this need to acquire and synthesize amino acids, a marked decrease in protease activity was detected. Most carbon-related enzymes suffered an increase under increased CO2. Overall, a shift in sediment extracellular enzymatic activity could be observed upon CO2 fertilization, mostly due to priming effects and not due to changes in the quality of carbon substrates, as shown by the sediment stable isotope signatures. The altered recycling activity of organic C, N, and P compounds may lead to an unbalance of these biogeochemical cycles, shifting the rhizosphere ecosystem function, with inevitable changes in the ecosystem services level. [ABSTRACT FROM AUTHOR]

The use of halophyte plants appears as a potential solution for degraded soil, food safety, freshwater scarcity, and coastal area utilization. These plants have been considered an alternative crop soilless agriculture for sustainable use of natural resources. There are few studies carried out with cultivated halophytes using a soilless cultivation system (SCS) that report their nutraceutical value, as well as their benefits on human health. The objective of this study was to evaluate and correlate the nutritional composition, volatile profile, phytochemical content, and biological activities of seven halophyte species cultivated using a SCS (Disphyma crassifolium L., Crithmum maritimum L., Inula crithmoides L., Mesembryanthemum crystallinum L., Mesembryanthemum nodiflorum L., Salicornia ramosissima J. Woods, and Sarcocornia fruticosa (Mill.) A. J. Scott.). Among these species, results showed that S. fruticosa had a higher content in protein (4.44 g/100 g FW), ash (5.70 g/100 g FW), salt (2.80 g/100 g FW), chloride (4.84 g/100 g FW), minerals (Na, K, Fe, Mg, Mn, Zn, Cu), total phenolics (0.33 mg GAE/g FW), and antioxidant activity (8.17 µmol TEAC/g FW). Regarding the phenolic classes, S. fruticosa and M. nodiflorum were predominant in the flavonoids, while M. crystallinum, C. maritimum, and S. ramosissima were in the phenolic acids. Moreover, S. fruticosa, S. ramosissima, M. nodiflorum, M. crystallinum, and I. crithmoides showed ACE-inhibitory activity, an important target control for hypertension. Concerning the volatile profile, C. maritimum, I. crithmoides, and D. crassifolium were abundant in terpenes and esters, while M. nodiflorum, S. fruticosa, and M. crystallinum were richer in alcohols and aldehydes, and S. ramosissima was richer in aldehydes. Considering the environmental and sustainable roles of cultivated halophytes using a SCS, these results indicate that these species could be considered an alternative to conventional table salt, due to their added nutritional and phytochemical composition, with potential contribution for the antioxidant and anti-hypertensive effects. [ABSTRACT FROM AUTHOR]

Trace metal element (TME) pollution is a major threat to plants, animals and humans. Agricultural products contaminated with metals may pose health risks for people; therefore, international standards have been established by the FAO/WHO to ensure food safety as well as the possibility of crop production in contaminated soils. This study aimed to assess the accumulating potential of aluminum and barium in the roots, shoots and fruits of Abelmoschus esculentus L., and their effect on growth and mineral nutrition. The content of proline and some secondary metabolites was also evaluated. After treating okra plants with aluminum/barium (0, 100, 200, 400 and 600 µM) for 45 days, the results showed that Al stimulated the dry biomass production, whereas Ba negatively affected the growth and the fructification yield. The okra plants retained both elements and exhibited a preferential accumulation in the roots following the sequence: roots > shoots > fruits, which is interesting for phytostabilization purposes. Al or Ba exposure induced a decline in mineral uptake (K, Ca, Mg, Zn and Fe), especially in roots and shoots. In order to cope with the stress conditions, the okra plants enhanced their proline and total phenol amounts, offering better adaptability to stress. [ABSTRACT FROM AUTHOR]

Trace metal elements (TMEs) are among the most important types of pollutants in the environment. Therefore, a precise determination of these contaminants in several environmental components is required for the safety assurance of living organisms. Spectroscopic analysis is an efficient technique employed to detect and determine TME contents in numerous samples. Hence, to achieve reliable and accurate results when using spectroscopic analysis, samples should be carefully prepared. In the present study, the comparison of eight digestion methods of five vegetal samples was carried out to quantify Cd, Mn, Al and Mg contents using the atomic absorption spectroscopy technique. According to the extraction techniques used in this study, results showed an outstanding difference in TME levels determined in the same vegetal sample. The results obtained indicated that the highest Mn concentrations were recorded when using the mixture of HNO3-HClO4 in the studied species: atriplex portulacoides, arthrocnemum indicum, olea europaea BCR-62, ulva lactuca and ulva lactuca BCR-279 compared to all other methods. Regarding the extraction of Cd, our results showed that heated extraction using different acids (HNO3-H2SO4-HClO4, HCl-HNO3, HNO3-HClO4, HNO3-H2SO4, HNO3-HCl-HClO4 and HNO3-HCl-H2SO4) was the most efficient in atriplex portulacoides, arthrocnemum indicum, olea europaea BCR-62, ulva lactuca and ulva lactuca BCR-279. Similarly, these heated acid digestion techniques (efficient for Cd) showed the highest levels of Al in atriplex portulacoides and arthrocnemum indicum. However, for the Mg extraction, our results revealed that the effectiveness of the method used depended on the plant species studied. Regarding these findings, the efficiency of metal quantification by AAS depends on the digestion procedure, the metallic ion to determine and the plant species. [ABSTRACT FROM AUTHOR]

Sodium Dodecyl Sulfate (SDS) is an anionic surfactant, extensively used in detergents, household and personal care products, as well as in industrial processes. The present study aimed to disclose the potential toxicological effects of SDS exposure under environmentally relevant concentrations (0, 0.1, 1, 3, and 10 mg L−1) on the physiology and biochemistry (photosynthesis, pigment, and lipid composition, antioxidative systems, and energy balance) of two marine autotrophs: the diatom Phaeodactylum tricornutum and the macroalgae Ulva lactuca. A growth rate (GR) reduction in P. tricornutum was observed with a classic dose-response effect towards the highest applied concentration, while a GR increase occurred in U. lactuca. Regarding photochemistry, the decrease in the fluorescence of the OJIP curves and laser-induced fluorescence allowed a better separation between SDS treatments in U. lactuca compared with P. tricornutum. Although all pigments significantly decreased in U. lactuca at the highest concentrations (except for antheraxanthin), no significant variations occurred in P. tricornutum. On the other hand, changes in fatty acid content were observed in P. tricornutum but not in U. lactuca. In terms of classical biomarker assessment, a dose-effect relationship of individual biomarkers versus SDS dose applied; U. lactuca displayed a higher number of biomarker candidates, including those in distinct metabolic pathways, increasing its usefulness for ecotoxicological applications. By evaluating the potential application of optical and biochemical traits, it was evident that the fatty acid profiles of the different exposure groups are excellent candidates in P. tricornutum, concomitant with the characteristics of this anionic surfactant. On the other hand, the results presented by laser-induced fluorescence and some parameters of PAM fluorometry in U. lactuca may be an advantage in the field, offering non-invasive, fast, easy-to-use, high-throughput screening techniques as excellent tools for ecotoxicology assessment. [ABSTRACT FROM AUTHOR]

Simple Summary: Pharmaceutical compounds, designed to act specifically in human or animal organisms, may have unknown and undesirable effects on non-target organisms in the environment. Untargeted metabolomic analysis uncovered several common effects of fluoxetine in diatoms and mammal cells, specifically in shared metabolic pathways, revealing a common mode of action, including serotonin re-uptake, which is the molecular target of this antidepressant. Moreover, FT-ICR-based metabolomic profiling was shown to be a powerful tool for the identification of potential biomarkers of exposure in diatom cells, highlighting its value in the ecotoxicology of marine organisms. The increased use of antidepressants, along with their increased occurrence in aquatic environments, is of concern for marine organisms. Although these pharmaceutical compounds have been shown to negatively affect marine diatoms, their mode of action in these non-target, single-cell phototrophic organisms is yet unknown. Using a Fourier-transform ion cyclotron-resonance mass spectrometer (FT-ICR-MS) we evaluated the effects of fluoxetine in the metabolomics of the model diatom Phaeodactylum tricornutum, as well as the potential use of the identified metabolites as exposure biomarkers. Diatom growth was severely impaired after fluoxetine exposure, particularly in the highest dose tested, along with a down-regulation of photosynthetic and carbohydrate metabolisms. Notably, several mechanisms that are normally down-regulated by fluoxetine in mammal organisms were also down-regulated in diatoms (e.g., glycerolipid metabolism, phosphatidylinositol signalling pathway, vitamin metabolism, terpenoid backbone biosynthesis and serotonin remobilization metabolism). Additionally, the present work also identified a set of potential biomarkers of fluoxetine exposure that were up-regulated with increasing fluoxetine exposure concentration and are of high metabolic significance following the disclosed mode of action, reinforcing the use of metabolomics approaches in ecotoxicology. [ABSTRACT FROM AUTHOR]

In the context of expanding fish production and complex distribution chains, traceability, provenance and food safety tools are becoming increasingly important. Here, we compare the elemental fingerprints of gilthead seabream (Sparus aurata) muscle from wild and different aquaculture productions (semi-intensive earth ponds and intensive sea cages from two locations) to confirm their origin and evaluate the concentrations of elements with regulatory thresholds (Cu, Hg, Pb and Zn). Using a chemometric approach based on multi-elemental signatures, the sample origin was determined with an overall accuracy of 90%. Furthermore, in a model built to replicate a real-case scenario where it would be necessary to trace the production method of S. aurata without reliable information about its harvesting location, 27 of the 30 samples were correctly allocated to their original production method (sea-cage aquaculture), despite being from another location. The concentrations of the regulated elements ranged as follows: Cu (0.140–1.139 mg/Kg), Hg (0–0.506 mg/Kg), Pb (0–2.703 mg/Kg) and Zn (6.502–18.807 mg/Kg), with only Pb presenting concentrations consistently above the recommended limit for human consumption. The present findings contribute to establishing elemental fingerprinting as a reliable tool to trace fish production methods and underpin seafood authentication. [ABSTRACT FROM AUTHOR]

Portugal mainland and Atlantic archipelagos (Madeira and Azores) provide a wide array of coastal ecosystems with varying typology and degrees of human pressure, which shape the microbial communities thriving in these habitats, leading to the development of microbial resistance traits. The samples collected on the Portuguese northeast Atlantic coast waters show an unequivocal prevalence of Bacteria over Archaea with a high prevalence of Proteobacteria, Cyanobacteria, Bacteroidetes and Actinobacteria. Several taxa, such as the Vibrio genus, showed significant correlations with anthropogenic pollution. These anthropogenic pressures, along with the differences in species diversity among the surveyed sites, lead to observed differences in the presence and resistance-related sequences' abundance (set of all metal and antibiotic resistant genes and their precursors in pathogenic and non-pathogenic bacteria). Gene ontology terms such as antibiotic resistance, redox regulation and oxidative stress response were prevalent. A higher number of significant correlations were found between the abundance of resistance-related sequences and pollution, inorganic pressures and density of nearby population centres when compared to the number of significant correlations between taxa abundance at different phylogenetic levels and the same environmental traits. This points towards predominance of the environmental conditions over the sequence abundance rather than the taxa abundance. Our data suggest that the whole resistome profile can provide more relevant or integrative answers in terms of anthropogenic disturbance of the environment, either as a whole or grouped in gene ontology groups, appearing as a promising tool for impact assessment studies which, due to the ubiquity of the sequences across microbes, can be surveyed independently of the taxa present in the samples. [ABSTRACT FROM AUTHOR]

Provenance and traceability are crucial aspects of seafood safety, supporting managers and regulators, and allowing consumers to have clear information about the origin of the seafood products they consume. In the present study, we developed an innovative spectral approach based on total reflection X-ray fluorescence (TXRF) spectroscopy to identify the provenance of seafood and present a case study for five economically relevant marine species harvested in different areas of the Atlantic Portuguese coast: three bony fish—Merluccius merluccius, Scomber colias, and Sparus aurata; one elasmobranch—Raja clavata; one cephalopod—Octopus vulgaris. Applying a first-order Savitzky–Golay transformation to the TXRF spectra reduced the potential matrix physical effects on the light scattering of the X-ray beam while maintaining the spectral differences inherent to the chemical composition of the samples. Furthermore, a variable importance in projection partial least-squares discriminant analysis (VIP-PLS-DA), with k − 1 components (where k is the number of geographical origins of each seafood species), produced robust high-quality models of classification of samples according to their geographical origin, with several clusters well-evidenced in the dispersion plots of all species. Four of the five species displayed models with an overall classification above 80.0%, whereas the lowest classification accuracy for S. aurata was 74.2%. Notably, about 10% of the spectral features that significantly contribute to class differentiation are shared among all species. The results obtained suggest that TXRF spectra can be used for traceability purposes in seafood species (from bony and cartilaginous fishes to cephalopods) and that the presented chemometric approach has an added value for coupling with classic TXRF spectral peak deconvolution and elemental quantification, allowing characterization of the geographical origin of samples, providing a highly accurate and informative dataset in terms of food safety. [ABSTRACT FROM AUTHOR]

The use of glyphosate-based herbicides (GBHs) worldwide has increased exponentially over the last two decades increasing the environmental risk to marine and coastal habitats. The present study investigated the effects of GBHs at environmentally relevant concentrations (0, 10, 50, 100, 250, and 500 μg·L−1) on the physiology and biochemistry (photosynthesis, pigment, and lipid composition, antioxidative systems and energy balance) of Ulva lactuca, a cosmopolitan marine macroalgae species. Although GBHs cause deleterious effects such as the inhibition of photosynthetic activity, particularly at 250 μg·L−1, due to the impairment of the electron transport in the chloroplasts, these changes are almost completely reverted at the highest concentration (500 μg·L−1). This could be related to the induction of tolerance mechanisms at a certain threshold or tipping point. While no changes occurred in the energy balance, an increase in the pigment antheraxanthin is observed jointly with an increase in ascorbate peroxidase activity. These mechanisms might have contributed to protecting thylakoids against excess radiation and the increase in reactive oxygen species, associated with stress conditions, as no increase in lipid peroxidation products was observed. Furthermore, changes in the fatty acids profile, usually attributed to the induction of plant stress response mechanisms, demonstrated the high resilience of this macroalgae. Notably, the application of bio-optical tools in ecotoxicology, such as pulse amplitude modulated (PAM) fluorometry and laser-induced fluorescence (LIF), allowed separation of the control samples and those treated by GBHs in different concentrations with a high degree of accuracy, with PAM more accurate in identifying the different treatments. [ABSTRACT FROM AUTHOR]

The present-day COVID-19 pandemic has led to the increasing daily use of antimicrobials worldwide. Triclosan is a manmade disinfectant chemical used in several consumer healthcare products, and thus frequently detected in surface waters. In the present work, we aimed to evaluate the effect of triclosan on diatom cell photophysiology, fatty acid profiles, and oxidative stress biomarkers, using the diatom Phaeodactylum tricornutum as a model organism. Several photochemical effects were observed, such as the lower ability of the photosystems to efficiently trap light energy. A severe depletion of fucoxanthin under triclosan application was also evident, pointing to potential use of carotenoid as reactive oxygen species scavengers. It was also observed an evident favouring of the peroxidase activity to detriment of the SOD activity, indicating that superoxide anion is not efficiently metabolized. High triclosan exposure induced high cellular energy allocation, directly linked with an increase in the energy assigned to vital functions, enabling cells to maintain the growth rates upon triclosan exposure. Oxidative stress traits were found to be the most efficient biomarkers as promising tools for triclosan ecotoxicological assessments. Overall, the increasing use of triclosan will lead to significant effects on the diatom photochemical and oxidative stress levels, compromising key roles of diatoms in the marine system. [ABSTRACT FROM AUTHOR]

Soils and water resources of our ecosystems may contain Barium (Ba), a toxic metal naturally existent in the Earth’s crust and also can be derived from recycled wastes produced of several anthropogenic activities. As a result of this fact, the accumulation of Ba in agriculture soils would increase to reach the crops and eventually end up in the human food chain. The purpose of this work was to study tolerance and accumulation abilities in Limbarda crithmoides and Helianthus annuus treated with increasing concentrations of barium (from 0 to 500 µM) for 45 days. In order to evaluate the response of these species to Ba stress, the biomass production, the water status, and the accumulation of the secondary metabolites, macronutrients, total inorganic nitrogen (TIN), and Ba in shoots and roots, as well as chlorophyll levels, and metal tolerance index of the entire plant were assessed. Results showed an increase in plant biomass production and tolerance index in the two species with increasing Ba concentration. A significant increase in polyphenols and flavonoids levels was also shown with no negative effect on the macronutrients and TIN; however, the latter were found reduced in roots of L. crithmoides. Chlorophylls also were not affected. An average of 3000 µg·g −1 DW of Ba was accumulated in each organ of L. crithmoides while H. annuus accumulated up to 1350 µg·g −1 DW in the shoots. Our findings proved that L. crithmoides and H. annuus were susceptible to tolerate Ba-induced stress with high levels of Ba accumulation in the aboveground parts as well as in the roots during the 45 days of the experiments. [ABSTRACT FROM AUTHOR]

Simple Summary: Expected climate change scenarios will have inevitable and important impacts on key foundation marine species such as seagrasses. This study was aimed to understand how the dwarf eelgrass Zostera noltii leaf lipid landscapes are altered under predicted ocean warming (+4 °C) and acidification (ΔpH 0.4) conditions. A severe reduction in the leaf total fatty acid (FA) content was observed in seagrasses individually exposed to hypercapnic or warming conditions, and this depletion was ameliorated under combined exposure to ocean warming and acidification conditions. The tested treatments also impacted the FA composition of all lipid classes, with warming exposure leading to decreases in polyunsaturated fatty acids (PUFAs). Galactolipid remodeling seems to have key roles in the physiological changes observed in seagrasses under these tested conditions, highlighting the higher impact of warming and that the proposed stress alleviation effect induced by increased water-dissolved CO2 availability. Neutral lipids were substantially increased under warming conditions, mainly with increases in C18 FA, impairing their use as substrates to maintain the osmotic balance of the cells. Nonetheless, the pace at which ocean warming is occurring can overcome the ameliorative capacity induced by higher CO2 availability, leaving seagrasses under severe heat stress beyond their lipid-remodeling capacity. Coastal seagrass meadows provide a variety of essential ecological and economic services, including nursery grounds, sediment stabilization, nutrient cycling, coastal protection, and blue carbon sequestration. However, these ecosystems are highly threatened by ongoing climatic change. This study was aimed to understand how the dwarf eelgrass Zostera noltii leaf lipid landscapes are altered under predicted ocean warming (+4 °C) and hypercapnic (ΔpH 0.4) conditions. Warming and hypercapnic conditions were found to induce a severe reduction in the leaf total fatty acid, though the combined treatment substantially alleviated this depletion. The lipid discrimination revealed a significant increase in the relative monogalactosyldiacylglycerol (MGDG) content in both hypercapnic and warming conditions, allied to plastidial membrane stabilization mechanisms. Hypercapnia also promoted enhanced phosphatidylglycerol (PG) leaf contents, a mechanism often associated with thylakoid reinvigoration. In addition to changing the proportion of storage, galacto- and phospholipids, the tested treatments also impacted the FA composition of all lipid classes, with warming exposure leading to decreases in polyunsaturated fatty acids (PUFAs); however, the combination of both stress conditions alleviated this effect. The observed galactolipid and phospholipid PUFA decreases are compatible with a homeoviscous adaptation, allowing for the maintenance of membrane stability by counteracting excessive membrane fluidity. Neutral lipid contents were substantially increased under warming conditions, especially in C18 fatty acids (C18), impairing their use as substrates for fatty acylated derivatives essential for maintaining the osmotic balance of cells. An analysis of the phospholipid and galactolipid fatty acid profiles as a whole revealed a higher degree of discrimination, highlighting the higher impact of warming and the proposed stress alleviation effect induced by increased water-dissolved CO2 availability. Still, it is essential to remember that the pace at which the ocean is warming can overcome the ameliorative capacity induced by higher CO2 availability, leaving seagrasses under severe heat stress beyond their lipid remodeling capacity. [ABSTRACT FROM AUTHOR]

Plant growth-promoting rhizobacteria (PGPR) can promote plant growth through mechanisms such as mineral phosphates solubilization, biological N2 fixation and siderophores and phytohormones production. The present work aims to evaluate the physiological fitness improvement by PGPR in Halimione portulacoides under mild and severe salt stress. PGPR-inoculated plants showed improved energy use efficiencies, namely in terms of the trapped and electron transport energy fluxes, and reduced energy dissipation. Allied to this, under mild stress, inoculated plants exhibited a significant reduction of the Na and Cl root concentrations, accompanied by a significant increase in K and Ca leaf content. This ion profile reshaping was intrinsically connected with an increased leaf proline content in inoculated plants. Moreover, bioaugmented plants showed an increased photoprotection ability, through lutein and zeaxanthin leaf concentration increase, allowing plants to cope with potentially photoinhibition conditions. Reduced Na leaf uptake in inoculated plants, apparently reduced the oxidative stress degree as observed by the superoxide dismutase and peroxidase activity reduction. Additionally, a reduced lipid peroxidation degree was observed in inoculated plants, while compared to their non-inoculated counterparts. These results, point out an important role of bioaugmentation in promoting plant fitness and improving salt tolerance, with a great potential for applications in biosaline agriculture and salinized soil restoration. [ABSTRACT FROM AUTHOR]

Rare earth elements (REEs) present a group of nonessential metals for the growth and development of plants. At high concentrations, they can induce internal stress and disturb the physiological and biochemical mechanisms in plants. The potential uptake of lanthanum (La) and cerium (Ce) by the horticultural plant Helianthus annuus and the effect of these elements on its growth, its absorption of macroelements, and the contents of phenolic compounds and flavonoids were assessed. The plants were exposed to 0, 1, 2.5, 5, and 10 µM of La and Ce for 14 days. The results showed a remarkable accumulation of the two REEs, especially in the roots, which was found to be positively correlated with the total phenolic compound and flavonoid content in the plant shoots and roots. The plant's growth parameter patterns (such as dry weight and water content); the levels of potassium, calcium, and magnesium; and the tolerance index varied with the concentrations of the two studied elements. According to the tolerance index values, H. annuus had more affinity to La than to Ce. Although these metals were accumulated in H. annuus tissues, this Asteraceae plant cannot be considered as a hyperaccumulator species of these two REEs, since the obtained REE content in the plant's upper parts was less than 1000 mg·Kg−1 DW. [ABSTRACT FROM AUTHOR]

The continuous progress of global manufacturing and anthropogenic activities has resulted in excessive environmental metallic pollution, particularly with rare earth elements (REEs) which have become a prevalent issue of global concern due to their high toxicity and widespread existence. REEs-contaminated soils could ruin agriculture by inducing plant physiology disturbances in various crops that are considered the principal link of the human food chain. The main purpose of the present work is to assess the phytotoxicity of two light REEs, lanthanum (La) and cerium (Ce), in Helianthus annuus after 14 days of exposure to different concentrations of La and Ce (0, 1, 2.5, 5, and 10 µM). Plants showed different variations in shoot and root lengths at the end of the trial period. The accumulation of photosynthetic pigments, such as chlorophylls and carotenoids, as well as the photosynthetic efficiency, the non-photochemical quenching, the photosynthetically active radiation, and the electron transport rate, increased in the two REE treatments. Hydrogen peroxide significantly increased in all applied concentrations of La and Ce. A significant increase in malondialdehyde content was noticed only when plants were exposed to 2.5 µM La and 10 µM Ce. Results also demonstrated that La and Ce induced an increase in the activity of superoxide dismutase, peroxidase, and catalase (only the highest concentration of La decreased catalase activity). The exposure to different REE concentrations induced the accumulation of La and Ce in the plants, mainly in roots. Helianthus annuus showed an effective resistance behaviour facing La- and Ce-induced stresses. [ABSTRACT FROM AUTHOR]

Soil contamination with petroleum-derived substances such as diesel fuel has become a major environmental threat. Phytoremediation is one of the most studied ecofriendly low-cost solutions nowadays and halophytes species has been proved to have potential as bio-tools for this purpose. The extent to which salinity influences diesel tolerance in halophytes requires investigation. A greenhouse experiment was designed to assess the effect of NaCl supply (0 and 85 mM NaCl) on the growth and photosynthetic physiology of Juncus acutus plants exposed to 0, 1 and 2.5% diesel fuel. Relative growth rate, water content and chlorophyll a derived parameters were measured in plants exposed to the different NaCl and diesel fuel combinations. Our results indicated that NaCl supplementation worsened the effects of diesel toxicity on growth, as diesel fuel at 2.5% reduced relative growth rate by 25% in the absence of NaCl but 80% in plants treated with NaCl. Nevertheless, this species grown at 0 mM NaCl showed a high tolerance to diesel fuel soil presence in RGR but also in chlorophyll fluorescence parameters that did not significantly decrease at 1% diesel fuel concentration in absence of NaCl. Therefore, this study remarked on the importance of knowing the tolerance threshold to abiotic factors in order to determine the bioremediation capacity of a species for a specific soil or area. In addition, it showed that NaCl presence even in halophytes does not always have a positive effect on plant physiology and it depends on the pollutant nature. [ABSTRACT FROM AUTHOR]

Featured Application: light emitting diodes (LED) illumination with different wavelengths can modulate diatom ca-rotenoid and fatty acid production. Diatoms are microscopic biorefineries producing value-added molecules, including unique pigments, triglycerides (TAGs) and long-chain polyunsaturated fatty acids (LC-PUFAs), with potential implications in aquaculture feeding and the food or biofuel industries. These molecules are utilized in vivo for energy harvesting from sunlight to drive photosynthesis and as photosynthetic storage products, respectively. In the present paper, we evaluate the effect of narrow-band spectral illumination on carotenoid, LC-PUFAs and TAG contents in the model diatom Phaeodactylum tricornutum. Shorter wavelengths in the blue spectral range resulted in higher production of total fatty acids, namely saturated TAGs. Longer wavelengths in the red spectral range increased the cell's content in Hexadecatrienoic acid (HTA) and Eicosapentaenoic acid (EPA). Red wavelengths induced higher production of photoprotective carotenoids, namely fucoxanthin. In combination, the results demonstrate how diatom value-added molecule production can be modulated by spectral light control during the growth. How diatoms could use such mechanisms to regulate efficient light absorption and cell buoyancy in the open ocean is discussed. [ABSTRACT FROM AUTHOR]

Featured Application: Merging physiological data with ecosystem services valuation provides valuable insights into the impact of climate change in coastal salt marsh systems. Salt marshes are valuable ecosystems, as they provide food, shelter, and important nursery areas for fish and macroinvertebrates, and a wide variety of ecosystem services for human populations. These ecosystem services heavily rely on the floristic composition of the salt marshes with different species conferring different service values and different adaptation and resilience capacities towards ecosystem stressors. Blue carbon, nitrogen, and phosphorous stocks are no exception to this, and rely on the interspecific differences in the primary production metabolism and physiological traits. Furthermore, these intrinsic physiological characteristics also modulate the species response to any environmental stressor, such as the ones derived from ongoing global changes. This will heavily shape transitional ecosystem services, with significant changes of the ecosystem value of the salt marshes in terms of cultural, provisioning, regulating, and supporting ecosystem services, with a special emphasis on the possible alterations of the blue carbon, nitrogen, and phosphorous stocks retained in these key environments. Thus, the need to integrate plant physiological characteristics and feedbacks towards the expected climate change-driven stressors becomes evident to accurately estimate the ecosystem services of the salt marsh community, and transfer these fundamental services into economic assets, for a fluid communication of the ecosystems value to stakeholders, decision and policy makers, and environmental management entities. [ABSTRACT FROM AUTHOR]

Featured Application: Application of non-invasive bio-optical techniques to evaluate the ecotoxicity of glyphosate-based pesticide in marine diatoms with confirmation by classical biochemical tools. Glyphosate is the main active component of the commercial formulation Roundup®, the most widely used chemical herbicide worldwide. However, its potential high toxicity to the environment and throughout trophic webs has come under increasing scrutiny. The present study aims to investigate the application of bio-optical techniques and their correlation to physiological and biochemical processes, including primary productivity, oxidative stress, energy balance, and alterations in pigment and lipid composition in Phaeodactylum tricornutum, a representative species of marine diatoms, using the case study of its response to the herbicide glyphosate-based Roundup® formulation, at environmentally relevant concentrations. Cultures were exposed to the herbicide formulation representing effective glyphosate concentrations of 0, 10, 50, 100, 250, and 500 μg L−1. Results showed that high concentrations decreased cell density; furthermore, the inhibition of photosynthetic activity was not only caused by the impairment of electron transport in the thylakoids, but also by a decrease of antioxidant capacity and increased lipid peroxidation. Nevertheless, concentrations of one of the plastidial marker fatty acids had a positive correlation with the highest concentration as well as an increase in total protein. Cell energy allocation also increased with concentration, relative to control and the lowest concentration, although culture growth was inhibited. Pigment composition and fatty acid profiles proved to be efficient biomarkers for the highest glyphosate-based herbicide concentrations, while bio-optical data separated controls from intermediate concentrations and high concentrations. [ABSTRACT FROM AUTHOR]

The stalked barnacle Pollicipes pollicipes is an abundant species on the very exposed rocky shore habitats of the Spanish and Portuguese coasts, constituting also an important economical resource, as a seafood item with high commercial value. Twenty-four elements were measured by untargeted total reflection X-ray fluorescence spectroscopy (TXRF) in the edible peduncle of stalked barnacles sampled in six sites along the Portuguese western coast, comprising a total of 90 individuals. The elemental profile of 90 individuals originated from several geographical sites (N = 15 per site), were analysed using several chemometric multivariate approaches (variable in importance partial least square discriminant analysis (VIP-PLS-DA), stepwise linear discriminant analysis (S-LDA), linear discriminant analysis (LDA), random forests (RF) and canonical analysis of principal components (CAP)), to evaluate the ability of each approach to trace the geographical origin of the animals collected. As a suspension feeder, this species introduces a high degree of background noise, leading to a comparatively lower classification of the chemometric approaches based on the complete elemental profile of the peduncle (canonical analysis of principal components and linear discriminant analysis). The application of variable selection approaches such as the VIP-PLS-DA and S-LDA significantly increased the classification accuracy (77.8% and 84.4%, respectively) of the samples according to their harvesting area, while reducing the number of elements needed for this classification, and thus the background noise. Moreover, the selected elements are similar to those selected by other random and non-random approaches, reinforcing the reliability of this selection. This untargeted analytical procedure also allowed to depict the degree of risk, in terms of human consumption of these animals, highlighting the geographical areas where these delicacies presented lower values for critical elements compared to the standard thresholds for human consumption. [ABSTRACT FROM AUTHOR]

The question of trace metal elements (TME) is still relevant and causes several environmental problems. Moreover, the digestion methods of TME have a significant impact on ecosystems. Sample preparation is an important step of any analytical procedure. In fact, defining the levels of TME in vegetal tissues requires various steps: drying samples, crushing, extraction, and dosage. The use of chemical extraction solutions can be put into question. Other than their lack of specificity, they are susceptible to provoking the redistribution and/or re-fixation of a part of the metallic ions between the various vegetal components. Our study aims to test the procedures of extraction that are specific for the metals Cu, Zn, Fe, Ca, Cr and Ba. Our results show an outstanding difference in the levels of TME derived from the same vegetal sample, according to the various techniques of extraction. In fact, cold extraction by nitric acid diluted at 10% is the most efficient way to put the ions of Cu, Zn, Fe and Ca into a solution. Meanwhile, the use of heated extraction using different acid digestion approaches is the most efficient for Cr and Ba solubilization from Atriplex portulacoides, Arthrocnemum indicum, Olea europaea BCR-62, Ulva lactuca and Ulva lactuca BCR-279. [ABSTRACT FROM AUTHOR]

Land salinization, resulting from the ongoing climate change phenomena, is having an increasing impact on coastal ecosystems like salt marshes. Although halophyte species can live and thrive in high salinities, they experience differences in their salt tolerance range, being this a determining factor in the plant distribution and frequency throughout marshes. Furthermore, intraspecific variation to NaCl response is observed in high-ranging halophyte species at a population level. The present study aims to determine if the environmental history, namely heavy metal pre-conditioning, can have a meaningful influence on salinity tolerance mechanisms of Spartina patens, a highly disperse grass invader in the Mediterranean marshes. For this purpose, individuals from pristine and heavy metal contaminated marsh populations were exposed to a high-ranging salinity gradient, and their intraspecific biophysical and biochemical feedbacks were analyzed. When comparing the tolerance mechanisms of both populations, S. patens from the contaminated marsh appeared to be more resilient and tolerant to salt stress, this was particularly present at the high salinities. Consequently, as the salinity increases in the environment, the heavy metal contaminated marsh may experience a more resilient and better adapted S. patens community. Therefore, the heavy metal pre-conditioning of salt mash populations appears to be able to create intraspecific physiological variations at the population level that can have a great influence on marsh plant distribution outcome. [ABSTRACT FROM AUTHOR]

Salicornia ramosissima J. Woods is a halophyte plant recognized as a promising natural ingredient and will eventually be recognized a salt substitute (NaCl). However, its shelf-life and applicability in several food matrices requires the use of drying processes, which may have an impact on its nutritional and functional value. The objective of this study was to evaluate the effect of oven and freeze-drying processes on the nutritional composition, volatile profile, phytochemical content, and bioactivity of S. ramosissima using several analytical tools (LC-DAD-ESI-MS/MS and SPME-GC-MS) and bioactivity assays (ORAC, HOSC, and ACE inhibition and antiproliferative effect on HT29 cells). Overall, results show that the drying process changes the chemical composition of the plant. When compared to freeze-drying, the oven-drying process had a lower impact on the nutritional composition but the phytochemical content and antioxidant capacity were significantly reduced. Despite this, oven-dried and freeze-dried samples demonstrated similar antiproliferative (17.56 mg/mL and 17.24 mg/mL, respectively) and antihypertensive (24.56 mg/mL and 18.96 mg/mL, respectively) activities. The volatile composition was also affected when comparing fresh and dried plants and between both drying processes: while for the freeze-dried sample, terpenes corresponded to 57% of the total peak area, a decrease to 17% was observed for the oven-dried sample. The oven-dried S. ramosissima was selected to formulate a ketchup and the product formulated with 2.2% (w/w) of the oven-dried plant showed a good consumer acceptance score. These findings support the use of dried S. ramosissima as a promising functional ingredient that can eventually replace the use of salt. [ABSTRACT FROM AUTHOR]

Research on biosaline agriculture has been increasing worldwide in recent years. In this respect, the Iberian halophyte diversity present a high-value ecological solution to be implemented for biosaline-based agroecosystems. The research on these halophytic species has been increasing worldwide and, in the recent years, especially in terms saline agriculture adaptation, osmophysiology and nutraceutical potential, highlighting the importance and potential of these species in terms of agrosolutions. The Mediterranean area has high biodiversity in terms of endemic halophytic vegetation (ca. 62 species), providing an alternative pool of potential new agricultural products to be cultivated in adverse conditions. Besides being highly diverse, most of these species are endemic and present a perennial life cycle with several applications in terms of food, forage, nutraceutical, feedstock and remediation. More specifically, the Iberian halophytic flora shows potential as resources of essential fatty acids, minerals and antioxidants—all very important for human and animal nutrition. Alongside the establishment of halophyte agroecological solutions is the provision of key ecosystem services, such as carbon sequestration and soil rehabilitation. Moreover, halophyte-based ecosystems provide additional recognized ecosystem services, beyond the final product production, by improving soil health, ecosystem biodiversity and storing large amounts of carbon, thereby increasing the ecosystem resilience to climate change and offering a green solution against climate change. [ABSTRACT FROM AUTHOR]

Simple Summary: In the past two decades, increasing attention has been directed to investigate the incidence and consequences of pharmaceuticals in the aquatic environment. Propranolol is a non-selective β-adrenoceptor blocker used in large quantities worldwide to treat cardiovascular conditions. Diatoms (model organism) exposed to this compound showed evident signs of oxidative stress, a significant reduction of the autotrophic O2 production and an increase in the heterotrophic mitochondrial respiration. Additionally, diatoms exposed to propranolol showed a consumption of its storage lipids. In ecological terms this will have cascading impacts in the marine trophic webs, where these organisms are key elements, through a reduction of the water column oxygenation and essential fatty acid availability to the heterotrophic organisms that depend on these primary producers. In ecotoxicological terms, diatoms photochemical and fatty acid traits showed to be potential good biomarkers for toxicity assessment of diatoms exposed to this widespread pharmaceutical compound. Present demographic trends suggest a rise in the contributions of human pharmaceuticals into coastal ecosystems, underpinning an increasing demand to evaluate the ecotoxicological effects and implications of drug residues in marine risk assessments. Propranolol, a non-selective β-adrenoceptor blocker, is used worldwide to treat high blood pressure conditions and other related cardiovascular conditions. Although diatoms lack β-adrenoceptors, this microalgal group presents receptor-like kinases and proteins with a functional analogy to the animal receptors and that can be targeted by propranolol. In the present work, the authors evaluated the effect of this non-selective β-adrenoceptor blocker in diatom cells using P. tricornutum as a model organism, to evaluate the potential effect of this compound in cell physiology (growth, lipids and energy metabolism and oxidative stress) and its potential relevance for marine ecosystems. Propranolol exposure leads to a significant reduction in diatom cell growth, more evident in the highest concentrations tested. This is likely due to the observed impairment of the main primary photochemistry processes and the enhancement of the mitochondrial respiratory activity. More specifically, propranolol decreased the energy transduction from photosystem II (PSII) to the electron transport chain, leading to an increase in oxidative stress levels. Cells exposed to propranolol also exhibited high-dissipated energy flux, indicating that this excessive energy is efficiently diverted, to some extent, from the photosystems, acting to prevent irreversible photoinhibition. As energy production is impaired at the PSII donor side, preventing energy production through the electron transport chain, diatoms appear to be consuming storage lipids as an energy backup system, to maintain essential cellular functions. This consumption will be attained by an increase in respiratory activity. Considering the primary oxygen production and consumption pathways, propranolol showed a significant reduction of the autotrophic O2 production and an increase in the heterotrophic mitochondrial respiration. Both mechanisms can have negative effects on marine trophic webs, due to a decrease in the energetic input from marine primary producers and a simultaneous oxygen production decrease for heterotrophic species. In ecotoxicological terms, bio-optical and fatty acid data appear as highly efficient tools for ecotoxicity assessment, with an overall high degree of classification when these traits are used to build a toxicological profile, instead of individually assessed. [ABSTRACT FROM AUTHOR]