1. Sibling sRNA RyfA1 Influences Shigella dysenteriae Pathogenesis.
- Author
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Fris, Megan E., Broach, William H., Klim, Sarah E., Coschigano, Peter W., Carroll, Ronan K., Caswell, Clayton C., and Murphy, Erin R.
- Subjects
NON-coding RNA ,SHIGELLOSIS ,PATHOGENIC bacteria ,VIRULENCE of bacteria ,MEMBRANE proteins - Abstract
Small regulatory RNAs (sRNAs) of Shigella dysenteriae and other pathogens are vital for the regulation of virulence-associated genes and processes. Here, we characterize RyfA1, one member of a sibling pair of sRNAs produced by S. dysenteriae. Unlike its nearly identical sibling molecule, RyfA2, predicted to be encoded almost exclusively by non-pathogenic species, the presence of a gene encoding RyfA1, or a RyfA1-like molecule, is strongly correlated with virulence in a variety of enteropathogens. In S. dysenteriae, the overproduction of RyfA1 negatively impacts the virulence-associated process of cell-to-cell spread as well as the expression of ompC, a gene encoding a major outer membrane protein important for the pathogenesis of Shigella. Interestingly, the production of RyfA1 is controlled by a second sRNA, here termed RyfB1, the first incidence of one regulatory small RNA controlling another in S. dysenteriae or any Shigella species. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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