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Glutathione S-transferases (GSTs) are members of a protein superfamily with diverse physiological functions, including cellular detoxification and protection against oxidative damage. However, there is limited research on GSTs responding to cadmium (Cd) stress. This study classified 46 GST genes in Dendrobium officinale (D. officinale) into nine groups using model construction and domain annotation. Evolutionary analysis revealed nine subfamilies with diverse physical and chemical properties. Prediction of subcellular localization revealed that half of the GST members were located in the cytoplasm. According to the expression analysis of GST family genes responding to Cd stress, DoGST5 responded significantly to Cd stress. Transient expression of DoGST5-GFP in tobacco leaves revealed that DoGST5 was localized in the cytoplasm. DoGST5 overexpression in Arabidopsis enhanced Cd tolerance by reducing Cd-induced H2O2 and O2− levels. These findings demonstrate that DoGST5 plays a critical role in enhancing Cd tolerance by balancing reactive oxygen species (ROS) levels, offering potential applications for improving plant adaptability to heavy metal stress. [ABSTRACT FROM AUTHOR]

The absence of some forms of non-verbal communication in virtual reality (VR) can make VR-based group discussions difficult even when a leader is assigned to each group to facilitate discussions. In this paper, we discuss if the sensor data from off-the-shelf VR devices can be used to detect opportunities for facilitating engaging discussions and support leaders in VR-based group discussions. To this end, we focus on the detection of suppressed speaking intention in VR-based group discussions by using personalized and general models. Our extensive analysis of experimental data reveals some factors that should be considered to enable effective feedback to leaders. In particular, our results show the benefits of combining the sensor data from leaders and low-engagement participants, and the usefulness of specific HMD sensor features. [ABSTRACT FROM AUTHOR]

While virtual reality (VR) technologies enable remote communication through the use of 3D avatars, it is often difficult to foster engaging group discussions without addressing the limitations to the non-verbal communication among distributed participants. In this paper, we discuss a technique to detect the intentions to speak in group discussions by tapping into intricate sensor data streams from VR headsets and hand-controllers. To this end, we developed a prototype VR group discussion app equipped with comprehensive sensor data-logging functions and conducted an experiment of VR group discussions (N = 24). We used the quantitative and qualitative experimental data to analyze participants' experiences of group discussions in relation to the temporal patterns of their different speaking intentions. We then propose a sensor-based mechanism for detecting speaking intentions by employing a sampling strategy that considers the temporal patterns of speaking intentions, and we verify the feasibility of our approach in group discussion settings. [ABSTRACT FROM AUTHOR]

Polygonatum cyrtonema (P. cyrtonema) is a valuable rhizome-propagating traditional Chinese medical herb. Polysaccharides (PCPs) are the major bioactive constituents in P. cyrtonema. However, the molecular basis of PCP biosynthesis in P. cyrtonema remains unknown. In this study, we measured the PCP contents of 11 wild P. cyrtonema germplasms. The results showed that PCP content was the highest in Lishui Qingyuan (LSQY, 11.84%) and the lowest in Hangzhou Lin'an (HZLA, 7.18%). We next analyzed the transcriptome profiles of LSQY and HZLA. Through a qRT-PCR analysis of five differential expression genes from the PCP biosynthesis pathway, phosphomannomutase, UDP-glucose 4-epimerase (galE), and GDP-mannose 4,6-dehydratase were determined as the key enzymes. A protein of a key gene, galE1, was localized in the chloroplast. The PCP content in the transiently overexpressed galE1 tobacco leaves was higher than in the wild type. Moreover, luciferase and Y1H assays indicated that PcWRKY31 and PcWRKY34 could activate galE1 by binding to its promoter. Our research uncovers the novel regulatory mechanism of PCP biosynthesis in P. cyrtonema and is critical to molecular-assisted breeding. [ABSTRACT FROM AUTHOR]

The interstitial neurons in the white matter of the human and non-human primate cortex share a similar developmental origin with subplate neurons and deep-layer cortical neurons. A subset of interstitial neurons expresses the molecular markers of subplate neurons, but whether interstitial neurons express cortical layer markers in the adult human brain remains unexplored. Here we report the expression of cortical layer markers in interstitial neurons in the white matter of the adult human brain, supporting the hypothesis that interstitial neurons could be derived from cortical progenitor cells. Furthermore, we found increased non-phosphorylated neurofilament protein (NPNFP) expression in interstitial neurons in the white matter of drug-resistant epilepsy patients. We also identified the expression of glutamatergic and g-aminobutyric acid (GABAergic) synaptic puncta that were distributed in the perikarya and dendrites of interstitial neurons. The density of glutamatergic and GABAergic synaptic puncta was increased in interstitial neurons in the white matter of drug-resistant epilepsy patients compared with control brain tissues with no history of epilepsy. Together, our results provide important insights of the molecular identity of interstitial neurons in the adult human white matter. Increased synaptic density of interstitial neurons could result in an imbalanced synaptic network in the white matter and participate as part of the epileptic network in drug-resistant epilepsy. [ABSTRACT FROM AUTHOR]

Difenoconazole (DFZ) is a broad-spectrum triazole fungicide that is widely utilized in agriculture. Although DFZ has been demonstrated to induce reproductive toxicity in aquatic species, its toxic effects on the mammalian reproductive system have yet to be fully elucidated. In vivo, male mice were administered 0, 20 or 40 mg/kg/d of DFZ via oral gavage for 35 days. Consequently, DFZ significantly decreased testicular organ coefficient, sperm count and testosterone levels, augmented sperm malformation rates, and elicited histopathological alterations in testes. TUNEL assay showed increased apoptosis in testis. Western blotting results suggested abnormally high expression of the sperm meiosis-associated proteins STRA8 and SCP3. The concentrations of retinoic acid (RA), retinaldehyde (RE), and retinol (ROL) were increased in the testicular tissues of DFZ-treated groups. The mRNA expression level of genes implicated in RA synthesis significantly increased while genes involved in RA catabolism significantly decreased. In vitro, DFZ reduced cell viability and increased RA, RE, and ROL levels in GC-2 cells. Transcriptome analysis revealed a significant enrichment of numerous terms associated with the RA pathway and apoptosis. The qPCR experiment verified the transcriptome results. In conclusion, our results indicate that DFZ exposure can disrupt RA signaling pathway homeostasis, and induce testicular injury in mice testes. [ABSTRACT FROM AUTHOR]

Polygonatum cyrtonema Hua is a traditional Chinese herb propagated using rhizomes, and excessive demand for seedlings and quality deterioration caused by rhizome propagation has highlighted that seed propagation may be an ideal solution to address these issues. However, the molecular mechanisms involved in P. cyrtonema Hua seed germination and emergence stages are not well understood. Therefore, in the present study, we performed transcriptomics combined with hormone dynamics during different seed germination stages, and 54,178 unigenes with an average length of 1390.38 bp (N50 = 1847 bp) were generated. Significant transcriptomic changes were related to plant hormone signal transduction and the starch and carbohydrate pathways. Genes related to ABA(abscisic acid), IAA(Indole acetic acid), and JA(Jasmonic acid) signaling, were downregulated, whereas genes related to ethylene, BR(brassinolide), CTK(Cytokinin), and SA(salicylic acid) biosynthesis and signaling were activated during the germination process. Interestingly, GA biosynthesis- and signaling-related genes were induced during the germination stage but decreased in the emergence stage. In addition, seed germination significantly upregulated the expression of genes associated with starch and sucrose metabolism. Notably, raffinose biosynthesis-related genes were induced, especially during the emergence stage. In total, 1171 transcription factor (TF) genes were found to be differentially expressed. Our results provide new insights into the mechanisms underlying P. cyrtonema Hua seed germination and emergence processes and further research for molecular breeding. [ABSTRACT FROM AUTHOR]

Based on CT experiments of the mechanical behavior of a certain sandstone, the safety zone, the damaging zone, and the fracture zone of geotechnical materials were defined, and the CT threshold standard of this zone was discussed and empirically selected. Mathematical morphological image measurement technology was used to analyze the CT information of each section, and the variation law of the sandstone CT number with the loading process and the conditions of the damaging zone was studied. According to the statistical results of the CT number of the safety zone, the damaging zone, and the fracture zone obtained at each loading stage, a statistical-damage variable based on the CT number was defined, and the equation of the damage evolution pertinent to this damage variable was established. On the basis of all these data, a constitutive model that can reflect the divisional damage-fracture of sandstone was constructed. The theoretical stress–strain curve and the measured curve were well fitted, demonstrating that the improved constitutive model could accurately reflect the failure of sandstone partitions. A qualitative analysis of CT scans and a quantitative analysis of the constitutive model were combined in this experiment, which has a certain reference value for the development of geotechnical engineering experiments. [ABSTRACT FROM AUTHOR]

Risk analysis using climate-induced yield losses (CIYL) extracted from long-term yield data have been recognized in China, but the research focusing on the time-series characteristics of risk and the circulation signals behind yield losses still remains incomplete. To address these challenges, a case study on winter wheat production in Henan province, north China was conducted by using annual series of yield in 17 cities during 1988–2017 and monthly series of 15 types of large-scale oceanic-atmospheric circulation indices (LOACI). A comprehensive risk assessment method was established by combining the intensity, frequency, and variability of CIYL and principal component analysis (PCA). The results showed that the westernmost Henan was identified as the area of higher-risk. PCA and Mann–Kendall trend tests indicated that the southern, northern, eastern, and western areas in Henan province were classified as having different annual CIYL variations in these four sub-regions; the decreasing trend of CIYL in northern area was the most notable. Since the 2000s, a significant decline in CIYL was found in each sub-region. It should be noted that the key LOACI, which includes Tropical Northern Atlantic Index (TNA), Western Hemisphere warm pool (WHWP), and Southern oscillation index (SOI), indicated significant CIYL anomalies in some months. Furthermore, the regional yield simulation results using linear regression for the independent variables of year and various LOACI were satisfactory, with the average relative error ranging from 3.48% to 6.87%. [ABSTRACT FROM AUTHOR]