1. Sequential Combination of Microwave- and Ultrasound-Assisted Extraction of Total Flavonoids from Osmanthus fragrans Lour. Flowers
- Author
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Zheng Xiangyang, Yu Jianfeng, Cui Zhengwei, Fu Jian, and Lou Qi
- Subjects
Materials science ,Sonication ,Oleaceae ,Liquid-Liquid Extraction ,Pharmaceutical Science ,Flowers ,01 natural sciences ,Article ,Analytical Chemistry ,lcsh:QD241-441 ,response surface methodology ,0404 agricultural biotechnology ,lcsh:Organic chemistry ,Liquid–liquid extraction ,total flavonoids ,Drug Discovery ,Response surface methodology ,Physical and Theoretical Chemistry ,Osmanthus fragrans Lour. flower ,microwave-assisted extraction ,ultrasound-assisted extraction ,Microwaves ,Flavonoids ,Chromatography ,biology ,Ethanol ,010405 organic chemistry ,Plant Extracts ,Organic Chemistry ,Extraction (chemistry) ,Osmanthus fragrans ,04 agricultural and veterinary sciences ,biology.organism_classification ,040401 food science ,0104 chemical sciences ,Chemistry (miscellaneous) ,Yield (chemistry) ,Solvents ,Molecular Medicine ,Ultrasonic sensor ,Factor Analysis, Statistical ,Microwave - Abstract
Microwave-assisted and ultrasound-assisted extraction assays were used to isolate total flavonoids (TF) from Osmanthus fragrans flowers. The effects of the solid-liquid ratio, ethanol concentration, microwave power, microwave extraction time, ultrasonic power and ultrasonic extraction time on the yield of TF were studied. A sequential combination of microwave- and ultrasound-assisted extraction (SC-MUAE) methods was developed, which was subsequently optimized by Box-Behnken design-response surface methodology (BBD-RSM). The interaction effects of the ethanol concentration (40-60%), microwave extraction time (5-7 min), ultrasonic extraction time (8-12 min) and ultrasonic power (210-430 W) on the yield of TF were investigated. The optimum operating parameters for the extraction of TF were determined to be as follows: ethanol concentration (48.15%), microwave extraction time (6.43 min), ultrasonic extraction time (10.09 min) and ultrasonic power (370.9 W). Under these conditions, the extraction yield of TF was 7.86 mg/g.
- Published
- 2017