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Simple Summary: In this study we describe the molecular and pathological characteristics of alpha- and gamma-herpesvirus infection of the central nervous system of stranded cetaceans and correlate them with viral load, immunohistochemical findings and biological data such as age, sex, and the presence of co-infections. The viruses (alpha- and gamma-herpesvirus) were detected in twelve out of 103 analysed stranded cetaceans and were associated with a wide range of histopathological lesions, as previously described for these and other species. In five out the twelve animals, lesions were severe enough (malacia, neuronal necrosis and neuronophagia) to cause death. Intranuclear inclusions bodies were present in brain tissue samples from half of the HV-positive animals, indicating that the injury was due to an infective agent belonging to a group of filterable viruses. These results are in accordance with immunohistochemical findings, as all the brain tissue samples with INIBs were immunolabeled with Anti-HSV1. Males, juveniles, and calves were predominantly infected among the analysed cetaceans and a 41.6% (5/12) incidence of co-infections in the brain was detected, with three animals co-infected with Dolphin Morbillivirus (DMV). In this study, we present, to the best of our knowledge, the first histopathological evidence of superinfection between HV and DMV pathogens in brain tissue. Herpesviruses are causative agents of meningitis and encephalitis in cetaceans, which are among the main leading known natural causes of death in these species. Brain samples from 103 stranded cetaceans were retrospectively screened for the presence of herpesvirus DNA in the brain. Molecular detection of Cetacean Morbillivirus was performed in HV positive brain cases. Histopathologic evaluation of brain samples included the presence or absence of the following findings (n = 7): meningitis, perivascular cuffings, microgliosis, intranuclear inclusion bodies, malacia, neuronal necrosis and neurophagic nodules, and haemorrhages. Histological evidence of the involvement of other etiological agents led to complementary analysis. We detected the presence of alpha and gamma-HVs in 12 out of 103 (11.6%) brain samples from stranded cetaceans of five different species: one bottlenose dolphin, six striped dolphins, three Atlantic spotted dolphins, one Cuvier's beaked whale, and one common dolphin. Pathogenic factors such as viral strain, age, sex, and the presence of co-infections were analysed and correlated with the brain histopathological findings in each case. Herpesvirus was more prevalent in males, juveniles, and calves and a 41.6% incidence of co-infections in the brain was detected in our study: three with Dolphin Morbillivirus, one with Staphilococcus aureus septicaemia and one with Brucella spp. [ABSTRACT FROM AUTHOR]

Simple Summary: Characterization, description, and geographical location of harmful bacterial agents in cetaceans are important for population surveillance and health monitoring around the world. This research compiles the pathologic features of nocardiosis in five free-ranging delphinids from the Canary Islands and Andalusia. All examined animals showed a disseminated pattern of infection with characteristic suppurative to pyogranulomatous lesions with thromboembolism in two or more organs. The obtained results provide the first record of N. otitidiscaviarum in cetaceans, the first account of N. farcinica in free-ranging dolphins, and confirmation of nocardiosis in central eastern Atlantic Ocean. We report the pathologic features of nocardiosis in five free-ranging delphinids from the Canary Islands and Andalusia, namely four striped dolphins (Stenella coerulealba) and one bottlenose dolphin (Tursiops truncatus). All animals had a multiorgan (disseminated) pattern of infection involving suppurative to pyogranulomatous and thromboembolic lesions in two or more organs. Most affected organs were (by decreasing order) lung, pulmonary lymph nodes, liver, kidney, adrenal glands, and central nervous system. Typical intralesional and intravascular branched and filamentous bacteria were highlighted by Grocott's methenamine silver and Gram stains. Bacterial analysis including 16S rRNA gene sequencing identified Nocardia farcinica in two striped dolphins and Nocardia otitidiscaviarum in one striped dolphin and the bottlenose dolphin. All dolphins tested (n = 4) for cetacean morbillivirus were negative; one dolphin had concurrent cutaneous herpesvirosis. These results provide the first record of N. otitidiscaviarum in cetaceans, the first account of N. farcinica in free-ranging dolphins, and confirmation of nocardiosis in central eastern Atlantic Ocean. These results expand the known geographic range of nocardiosis in cetaceans. [ABSTRACT FROM AUTHOR]