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(1) Background: Cold exposure induces metabolic adaptations that can promote health benefits, including increased energy disposal due to lipid mobilization in adipose tissue (AT). This study aims to identify easily measurable biomarkers mirroring the effect of cold exposure on AT. (2) Methods: Transcriptomic analysis was performed in peripheral blood mononuclear cells (PBMCs) and distinct AT depots of two animal models (ferrets and rats) exposed to cold, and in PBMCs of cold-exposed humans. (3) Results: One week of cold exposure (at 4 °C) affected different metabolic pathways and gene expression in the AT of ferrets, an animal model with an AT more similar to humans than that of rodents. However, only one gene, Tlcd4 , was affected in the same way (overexpressed) in aortic perivascular and inguinal AT depots and in PBMCs, making it a potential biomarker of interest. Subsequent targeted analysis in rats showed that 1 week at 4 °C also induced Tlcd4 expression in brown AT and PBMCs, while 1 h at 4 °C resulted in reduced Tlcd4 mRNA levels in retroperitoneal white AT. In humans, no clear effects were observed. Nevertheless, decreased PBMC TLCD4 expression was observed after acute cold exposure in women with normal weight, although this effect could be attributed to short-term fasting during the procedure. No effect was evident in women with overweight or in normal-weight men. (4) Conclusions: Our results obtained for different species point toward TLCD4 gene expression as a potential biomarker of cold exposure/fat mobilization that could tentatively be used to address the effectiveness of cold exposure-mimicking therapies.

Chronic Myeloid Leukemia (CML) originates in a leukemic stem cell that resides in the bone marrow microenvironment, where they coexist with cellular and non-cellular elements. The vascular microenvironment has been identified as an important element in CML development since an increase in the vascularization has been suggested to be related with poor prognosis; also, using murine models, it has been reported that bone marrow endothelium can regulate the quiescence and proliferation of leukemic stem and progenitor cells. This observation, however, has not been evaluated in primary human cells. In this report, we used a co-culture of primitive (progenitor and stem) CML cells with endothelial colony forming cells (ECFC) as an in vitro model to evaluate the effects of the vascular microenvironment in the leukemic hematopoiesis. Our results show that this interaction allows the in vitro maintenance of primitive CML cells through an inflammatory microenvironment able to regulate the proliferation of progenitor cells and the permanence in a quiescent state of leukemic stem cells., Competing Interests: The authors confirm that there are no conflicts of interest.

The impact of the lockdown, during the period from March to June in 2020, upon the air quality of the Basque Country in northern Spain is analyzed. The evaluation accounts for the meteorology of the period. Daily and sub-daily analysis of aerosol and ozone records show that the territory was repeatedly affected by episodes of pollutants from outer regions. Three episodes of PM 10 and ten of PM 2.5 were caused by transported anthropogenic European sulfates, African dust, and wildland fires. The region, with a varied orographic climatology, shows high and diverse industrial activity. Urban and interurban road traffic of the region decreased by 49% and 53%, respectively, whereas industrial activity showed a lower reduction of 20%. Consequently, the average concentrations of NO 2 in the cities during the period fell to 12.4 µg·m -3 (-45%). Ozone showed up to five exceedances of the WHOAQG for the daily maximum 8-h average in both rural and urban sites, associated with transport through France and the Bay of Biscay, under periods of European blocking anticyclones. However, averages showed a moderate decrease (-11%) in rural environments, in line with the precursor reductions, and disparate changes in the cities, which reproduced the weekend effect of their historical records. The PM 10 decreased less than expected (-10% and -21%, in the urban and rural environments, respectively), probably caused by the modest decrease of industrial activity around urban sites and favorable meteorology for secondary aerosol formation, which could also influence the lower changes observed in the PM 2.5 (-1% and +3% at the urban and rural sites, respectively). Consequently, in a future low NOx traffic emission scenario, the inter-regional PM and ozone control will require actions across various sectors, including the industry and common pollution control strategies.

Unspecific peroxygenases (UPOs) are highly promiscuous biocatalyst with self-sufficient mono(per)oxygenase activity. A laboratory-evolved UPO secreted by yeast was covalently immobilized in activated carriers through one-point attachment. In order to maintain the desired orientation without compromising the enzyme's activity, the S221C mutation was introduced at the surface of the enzyme, enabling a single disulfide bridge to be established between the support and the protein. Fluorescence confocal microscopy demonstrated the homogeneous distribution of the enzyme, regardless of the chemical nature of the carrier. This immobilized biocatalyst was characterized biochemically opening an exciting avenue for research into applied synthetic chemistry.