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Catalase can decompose hydrogen peroxide in plants under controlled and stressed conditions. In a stressed environment, an increase in hydrogen peroxide production makes catalase activity a major defense system for plant growth. The current study was conducted to evaluate the catalase activity of the pre-isolated and identified bacterial strains Bacillus aryabhattai (AN30), Bacillus megaterium (AN24), Bacillus megaterium (AN31) and Bacillus megaterium (AN35) and their potential for rice seedling growth promotion. These strains were characterized for quantitative catalase, urease, siderophore and exopolysaccharide production using LB media. Subsequently, the effectiveness of these strains was checked by quantifying the catalase activity in the rhizosphere, roots and shoots of rice seedlings. The secretion of organic and phenolic compounds produced by the tested strains in liquid culture was also investigated. Plant growth parameters were also studied in a growth room trial. Our results showed that the strain AN24 showed the maximum catalase activity (1.36 mol cm−1), urease activity (1.35 mol cm−1) and exopolysaccharide (4.20 µg mL−1) and siderophore (2.32%) production in LB media. All tested strains showed significantly higher catalase activity in soil compared to the control. Among sole applications, strain AN24 showed better results; however, the consortium application of strains AN24 + AN30 + AN35 + AN31 showed the maximum improvement in dry biomass, shoot and root length, and increase in catalase activity of rice seedlings. The results showed that a consortium of these Bacillus strains with catalase activity has greater potential to enhance the antioxidant defense system and growth promotion of rice seedlings. However, further experimentation under natural conditions is required before using these strains as potential bioinoculants for improving rice growth and yield. [ABSTRACT FROM AUTHOR]

Green leafy vegetables, such as Vigna unguiculata, Brassica oleraceae, and Solanum scabrum, are important sources of vitamins A, B1, and C. Although vitamin deficiencies considerably affect human health, not much is known about the effects of changing soil and climate conditions on vegetable vitamin concentrations. The effects of high or low soil fertility and three drought intensities (75%, 50%, and 25% pot capacity) on three plant species were analysed (n = 48 pots) in a greenhouse trial. The fresh yield was reduced in all the vegetables as a result of lower soil fertility during a severe drought. The vitamin concentrations increased with increasing drought stress in some species. Regardless, the total vitamin yields showed a net decrease due to the significant biomass loss. Changes in vitamin concentrations as a result of a degrading environment and increasing climate change events are an important factor to be considered for food composition calculations and nutrient balances, particularly due to the consequences on human health, and should therefore be considered in agricultural trials. [ABSTRACT FROM AUTHOR]

The diversification of biomass resources is key to the transition towards a bioeconomy. Acrocomia spp., a neotropical genus of palms, is an example of plants' diversity potential for a sustainable bioeconomy. Acrocomia's adaptability to environments outside rainforests, its specific fruit properties and high yields has generated the interest of researchers and entrepreneurs, triggering its introduction as a multipurpose oil crop. Developing sustainability-oriented and knowledge-based acrocomia value webs requires a collaborative, systems approach from the outset. Fostering an inter- and transdisciplinary dialogue on acrocomia through a participatory workshop with both academic and non-academic actors contributed to this endeavor. This allowed the identification of priorities, knowledge gaps, and stakeholder roles, and served as the basis for the co-creation of a research and development roadmap. Key steps for the introduction of acrocomia include intertwined technical aspects relating to the development of planting material, cultivation systems, processing technologies and applications, market entry, and value web governance aspects. A broad collaboration among scientists, the public and private sectors, farmers, and civil society, is required for the development of acrocomia value webs. The incorporation of sustainability and a consideration of context in the design and development phases are fundamental to fostering the sustainable performance of acrocomia value webs. [ABSTRACT FROM AUTHOR]

Fruits are micronutrient-rich sources which are often underrepresented in children's diets. More insights into the determinants of children's fruit consumption are needed to improve nutrition education in Teso South Sub-County, Kenya. A multiphase mixed method study was applied among 48 farm households with children 0–8 years of age. A market survey together with focus group discussions were used to design a formative research approach including qualitative and quantitative data collection methods. The unavailability of fruits and the inability to plant fruit trees in the homesteads were the main challenges to improve fruit consumption behaviour, although a number of different fruit species were available on the market or in households. Perceived shortage of fruits, financial constraints to purchase fruits and taste were important barriers. Fruits as snacks given between meals was perceived as helpful to satisfy children. The mean number of fruit trees in the homesteads was positively associated with fruit consumption. Field trials are needed to test how best fruit trees within home gardens and on farms can be included, acknowledging limited space and constraints of households with young children. This should be combined with nutrition education programs addressing perceptions about the social and nutrient value of fruits for children. [ABSTRACT FROM AUTHOR]

Globally, governments and agricultural organizations implement policies and programs reflected in interventions such as input subsidies, extension services for modern inputs and training, which either enable or hinder agricultural diversification. A study was carried out from 2016 to 2019 in Lilongwe district, Malawi, to determine the influence of policies, programs and interventions on agricultural diversification. The study was using a mixed-method sequential explanatory design. It involved a literature review, interviews with 424 male and female smallholder farmers, 35 demonstration plots on agricultural diversification, 27 focus group discussions with farmers, and 17 key informant interviews with stakeholder representatives. The literature review showed that the 2016 Malawi agricultural policy already prioritized agricultural diversification as it included a policy statement and policy objective on agricultural diversification. This study found that stakeholders, namely the Ministry of Agriculture, Total Land Care, National Smallholder Farmers Association of Malawi, School of Agriculture for Family Independence, and Trustees of Agriculture Promotion Program, were promoting agricultural diversification. They did it by (i) organizing farmers into groups for agricultural diversification activities, (ii) provision of extension advice, and (iii) providing inputs for different crops as well as livestock either for free, on pass-on, or through loans. However, interventions were on small scale, had not fully addressed hindrances to agricultural diversification, were mostly supply-driven, and the interventions themselves faced sustainability-threatening problems such as failure to pass on livestock and seeds. The agricultural policy was thus not sufficiently translated into widespread programs and interventions to foster improvement in agricultural diversification. As such, we suggest re-channeling of funding from promoting mono-cropping to agricultural diversification by broadening the crop and livestock focus of advisory services, enabling farmer organization initiatives and community engagement for farmers to solve most diversification challenges on their own. [ABSTRACT FROM AUTHOR]

Intensive land use with inappropriate land management is directly degrading South Asian uplands. A field trial was carried out on the uplands of Western Thailand with a 25% slope to examine the effect of land use management on soil loss for sustainable crop production during two consecutive years (2010–2011). Various cropping systems with soil conservation practices were compared to maize sole cropping (MSC). Results revealed that soil loss was at a minimum in the intercropping system of maize-chili-hedgerows with minimum tillage and fertilization that was 50% to 61% and 60% to 81% less than MSC and the bare soil plot during both years, respectively. Yield advantage was at its maximum, as indicated by the highest land equivalent ratios of 1.28 and 1.21 during 2010 and 2011, respectively, in maize-chili-hedgerows-intercropping with minimum tillage and fertilization. The highest economic returns (5925 and 1058 euros ha−1 during 2010 and 2011, respectively) were also obtained from maize-chili-hedgerows-intercropping with minimum tillage and fertilization. Chili fresh fruit yield was maximum in the chili alone plot during both years due to the greater area under cultivation compared with intercropping. Maize-chili-hedgerows with minimum tillage and fertilization reduced soil loss and increased land productivity and net returns, indicating its promising features for sustainable crop production on uplands. [ABSTRACT FROM AUTHOR]

Arid and semi-arid regions are characterized by high temperature and low rainfall, leading to degraded agricultural soils of alkaline calcareous nature with low organic matter contents. Less availability of indigenous nutrients and efficacy of applied fertilizers are the major issues of crop production in these soils. Biochar application, in combination with plant growth promoting rhizobacteria with the ability to solubilize nutrients, can be an effective strategy for improving soil health and nutrient availability to crops under these conditions. Experiments were planned to evaluate the impact of biochar obtained from different sources in combination with acid-producing, nutrient-solubilizing Bacillus sp. ZM20 on soil biological properties and growth of maize (Zea mays L.) crops under natural conditions. Various biochar treatments, viz. wheat (Triticum aestivum L.) straw biochar, Egyptian acacia (Vachellia nilotica L.) biochar, and farm-yard manure biochar with and without Bacillus sp. ZM20, were used along with control. Soil used for pot and field trials was sandy loam in texture with poor water holding capacity and deficient in nutrients. Results of the pot trial showed that fresh and dry biomass, 1000 grain weight, and grain yield was significantly improved by application of biochar of different sources with and without Bacillus sp. ZM20. Application of biochar along with Bacillus sp. ZM20 also improved soil biological properties, i.e., soil organic matter, microbial biomass carbon, ammonium, and nitrate nitrogen. It was also observed that a combined application of biochar with Bacillus sp. ZM20 was more effective than a separate application of biochar. The results of wheat straw biochar along with Bacillus sp. ZM20 were better as compared to farm-yard manure biochar and Egyptian acacia biochar. Maximum increase (25.77%) in grain yield was observed in the treatment where wheat straw biochar (0.2%) was applied in combination with Bacillus sp. ZM20. In conclusion, combined application of wheat straw biochar (0.2%) inoculated with Bacillus sp. ZM20 was the most effective treatment in improving the biological soil properties, plant growth, yield, and quality of maize crop as compared to all other treatments. [ABSTRACT FROM AUTHOR]

Chickpea is an important leguminous crop that improves soil fertility through atmospheric nitrogen fixation with the help of rhizobia present in nodules. Non-rhizobia endophytes are also capable of inducing nodulation and nitrogen fixation in leguminous crops. The aim of the current study was to isolate, characterize and identify the non-rhizobia endophytic bacterial strains from root nodules of chickpea. For this purpose, more than one hundred isolates were isolated from chickpea root nodules under aseptic conditions and were confirmed as endophytes through re-isolating them from root nodules of chickpea after their inoculation. Nineteen confirmed endophytic bacterial strains revealed significant production of indole acetic acid (IAA) both in presence and absence of L-tryptophan and showed their ability to grow under salt, pH and heavy metal stresses. These strains were evaluated for in vitro plant growth promoting (PGP) traits and results revealed that seven strains showed solubilization of P and colloidal chitin along with possessing catalase, oxidase, urease and chitinase activities. Seven P-solubilizing strains were further evaluated in a jar trial to explore their potential for promoting plant growth and induction of nodulation in chickpea roots. Two endophytic strains identified as Paenibacillus polymyxa ANM59 and Paenibacillus sp. ANM76 through partial sequencing of the 16S rRNA gene showed the maximum potential during in vitro PGP activities and improved plant growth and nodulation in chickpea under the jar trial. Use of these endophytic strains as a potential biofertilizer can help to reduce the dependence on chemical fertilizers while improving crop growth and soil health simultaneously. [ABSTRACT FROM AUTHOR]