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The present study focuses on the dynamic system identification of the Suleiman Mosque minaret in the medieval city of Rhodes, Greece. Suleiman Mosque was built in 1522 at the site of the destroyed Christian Church of the Apostles. First, we performed sets of ambient vibration measurements at the minaret of the monument. Based on these data, we calculated the eigenproperties of the minaret. Next, we modeled the monument in three dimensions, using the finite element method. Six numerical models were considered. Model Ι is the simplest one (isolated, fixed base minaret). Model VI is the most complicated one (simulation of the whole mosque also considering soil–structure interaction and foundation flexibility). The calculated predominant periods and mode shapes of Models I–VI are validated against the microtremor field measurements, recorded on the minaret's two floors and ground level. We elaborate on the reliability of finite element models for earthquake response evaluation, considering soil–structure interaction and foundation flexibility on the mode shape eigenfrequencies. Additionally, we discuss the seismic response of the minaret compared to the whole monument. We observed no significant difference in the first two modes of response, implying that the minaret's dynamic behavior is slightly affected by the entire mosque's presence. [ABSTRACT FROM AUTHOR]