1. Genome Characterization of Mammalian Orthoreovirus and Porcine Epidemic Diarrhea Virus Isolated from the Same Fattening Pig.
- Author
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Li, Xiaoxuan, Zhao, Jiakai, Li, Jingjie, Xiri, Yangzong, Liu, Zhixiang, Zhao, Qin, and Sun, Yani
- Subjects
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PORCINE epidemic diarrhea virus , *GENOMICS , *VIRAL genomes , *COMMUNICABLE diseases , *INTESTINAL diseases - Abstract
Simple Summary: Mammalian orthoreovirus (MRV) and porcine epidemic diarrhea virus (PEDV) are two causative agents of pig diarrhea and contagious intestinal disease. Generally, PEDV infection causes severe diarrhea, dehydration, and even death in piglets, and MRV causes mild gastroenteritis and respiratory diseases. In this study, the co-infection of MRV2 and PEDV was first determined in fattening pigs with diarrheal diseases and the two viral genome sequences were obtained from the same pig. The phylogenetic analysis based on the complete genome showed that the MRV2 strain was a reassortment of human and porcine MRV, and the PEDV strain belonged to the G2a genotype. This will help to better understand PEDV and MRV and provide a scientific basis for the development of effective prevention and control strategies. In 2020, severe diarrhea occurred in four-month-old fattening pigs from nine farms in Shandong Province, China. Fecal samples were collected from diseased pigs and tested by PCR for the presence of mammalian orthoreovirus (MRV), porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV), porcine rotavirus A (PoRVA), transmissible gastroenteritis virus (TGEV), porcine kobuvirus (PKV), and pseudorabies virus (PRV). The viral RNA of MRV and PEDV was detected in the fecal samples. The genome sequences of MRV and PEDV were successfully amplified from the same fecal sample. Genomic and phylogenetic analysis showed that the MRV isolate named MRV2-SD/2020 belongs to serotype 2 MRV (MRV2) and may originate from the reassortment of human and porcine MRVs. Compared with other MRV2 strains, there were four other unique amino acid mutations (L274I, F302L, V347I, and T440M) in the receptor binding region. For the PEDV isolate named PEDV-SD/2020, the nearly complete genome was amplified from the positive fecal samples. Phylogenetic analysis showed that it was classified into the G2a genotype. Compared with CV777 and other PEDV variant strains, its spike (S) protein exhibited two unique mutations (S663T and L966M). This study first reports the co-infection of PEDV and MRV2 in the pigs and provides a new direction for the prevention and control of the diarrhea diseases. [ABSTRACT FROM AUTHOR]
- Published
- 2025
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