1. Gene Expression of Type VI Secretion System Associated with Environmental Survival in Acidovorax avenae subsp. avenae by Principle Component Analysis
- Author
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Zhouqi Cui, Yangli Wang, Guan-Lin Xie, Kaleem Ullah Kakar, Bin Li, Guoqiang Jin, Guochang Sun, and Mohammad Reza Ojaghian
- Subjects
Burkholderia seminalis ,Gene mutation ,Environment ,Catalysis ,Article ,Microbiology ,gene knockout ,principle component analysis ,Inorganic Chemistry ,lcsh:Chemistry ,Comamonadaceae ,T6SS gene expression ,stress ,Stress, Physiological ,Gene expression ,Physical and Theoretical Chemistry ,Molecular Biology ,Gene ,Pathogen ,lcsh:QH301-705.5 ,Spectroscopy ,Type VI secretion system ,Principal Component Analysis ,Microbial Viability ,biology ,Acidovorax ,Effector ,Organic Chemistry ,food and beverages ,General Medicine ,Gene Expression Regulation, Bacterial ,Type VI Secretion Systems ,biology.organism_classification ,in vivo ,co-culture ,Computer Science Applications ,lcsh:Biology (General) ,lcsh:QD1-999 ,Genes, Bacterial ,Gene-Environment Interaction - Abstract
Valine glycine repeat G (VgrG) proteins are regarded as one of two effectors of Type VI secretion system (T6SS) which is a complex multi-component secretion system. In this study, potential biological roles of T6SS structural and VgrG genes in a rice bacterial pathogen, Acidovorax avenae subsp. avenae (Aaa) RS-1, were evaluated under seven stress conditions using principle component analysis of gene expression. The results showed that growth of the pathogen was reduced by H2O2 and paraquat-induced oxidative stress, high salt, low temperature, and vgrG mutation, compared to the control. However, pathogen growth was unaffected by co-culture with a rice rhizobacterium Burkholderia seminalis R456. In addition, expression of 14 T6SS structural and eight vgrG genes was significantly changed under seven conditions. Among different stress conditions, high salt, and low temperature showed a higher effect on the expression of T6SS gene compared with host infection and other environmental conditions. As a first report, this study revealed an association of T6SS gene expression of the pathogen with the host infection, gene mutation, and some common environmental stresses. The results of this research can increase understanding of the biological function of T6SS in this economically-important pathogen of rice.
- Published
- 2015