1. Expression Profiling of Circulating MicroRNAs in Canine Myxomatous Mitral Valve Disease.
- Author
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Li Q, Freeman LM, Rush JE, and Laflamme DP
- Subjects
- Animals, Dog Diseases genetics, Dog Diseases pathology, Dogs, Female, Heart Valve Diseases blood, Heart Valve Diseases diagnosis, Heart Valve Diseases genetics, Male, Mitral Valve Prolapse blood, Mitral Valve Prolapse diagnosis, Mitral Valve Prolapse genetics, Real-Time Polymerase Chain Reaction, Biomarkers metabolism, Dog Diseases blood, Heart Valve Diseases veterinary, MicroRNAs blood, MicroRNAs genetics, Mitral Valve Prolapse veterinary
- Abstract
MicroRNAs (miRNAs) are small non-coding RNAs that have shown promise as noninvasive biomarkers in cardiac disease. This study was undertaken to investigate the miRNA expression profile in dogs with myxomatous mitral valve disease (MMVD). 277 miRNAs were quantified using RT-qPCR from six normal dogs (American College of Veterinary Internal Medicine Stage A), six dogs with MMVD mild to moderate cardiac enlargement (ACVIM Stage B1/B2) and six dogs with MMVD and congestive heart failure (ACVIM Stage C/D). Eleven miRNAs were differentially expressed (False Discovery Rate < 0.05). Dogs in Stage B1/B2 or C/D had four upregulated miRNAs, including three cfa-let-7/cfa-miR-98 family members, while seven others were downregulated, compared to Stage A. Expression of six of the 11 miRNAs also were significantly different between dogs in Stage C/D and those in Stage B1/B2. The expression changes were greater as disease severity increased. These miRNAs may be candidates for novel biomarkers and may provide insights into genetic regulatory pathways in canine MMVD.
- Published
- 2015
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