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Simple Summary: During the awakening phase from general anesthesia, phases of agitation, excitement, vocalization, or violent movements are present in both human and veterinary medicine. Treatment for this phase involves the administration of sedatives such as alpha-2 agonist drugs that represents an established practice in equine anesthesia recovery. Dexmedetomidine is an alpha-2 agonist with sedative properties commonly used in veterinary clinical practice as a preanesthetic agent. The possibility of preventing these episodes of agitated awakening with the preventive administration of dexmedetomidine in canine patients has been studied in different populations. The objective of our study was to evaluate the quality of recovery from general anesthesia in dogs with the administration of two low doses of intravenous dexmedetomidine. The purpose of this study was to evaluate the quality of recovery from general anesthesia with the administration of two low doses of dexmedetomidine in canine patients. For this blind randomized clinical trial study, 30 dogs undergoing general anesthesia for diagnostic procedures or elective surgery (ovariectomy/castration) were included. The patients were randomly divided into three groups, and at the end of anesthesia, they received a bolus of dexmedetomidine at 1 mcg/kg IV (D1), or a bolus of dexmedetomidine at 0.5 mcg/kg (D0.5), or a bolus of NaCl, in a total of 0.5 mL of solution for all three groups. After administration of the bolus, the anesthetist monitored the patients every 5 min by measuring heart rate, systolic and mean blood pressure, respiratory rate, and oxygen saturation. The quality of recovery was also assessed using 4 different scales. The extubation time, time of headlift, and standing position were also recorded. Both groups receiving dexmedetomidine had better awakening and a lower incidence of delirium when compared to saline administration. The heart rate was lower, while the systolic pressure was higher in the two groups D1 and D0.5 compared to the NaCl with a low presence of atrioventricular blocks. The extubation time resulted significantly higher in the D1 (17 ± 6 min) compared to the D0.5 (10 ± 4 min) and NaCl (8 ± 3 min) (p < 0.0001); the headlift time D1 (25 ± 10 min) resulted significantly longer than the NaCl group (11 ± 5 min) (p = 0.0023) but not than the D0.5 (18 ± 9 min). No significant differences were found among the three groups for standing positioning (D1 50 ± 18 min, D0.5 39 ± 22 min, NaCl 28 ± 17 min). The preventive administration of a bolus of dexmedetomidine at a dosage of 0.5 mcg/kg or 1 mcg/kg IV during the recovery phase improves the quality of recovery in patients undergoing general anesthesia. [ABSTRACT FROM AUTHOR]

Simple Summary: Gastric ulcers are quite common in horses with the highest prevalence found in racehorses. Historical horseraces are traditional horseriding competitions popular in Italy that can be divided into two different types: speed races and jousting tournaments. Anglo-Arabian and Thoroughbreds are used, with several restrictions present in the regulation of the specific competition, pertaining to the breed of the horse, training, and preparation for the competitions (i.e., clinical examinations, qualifiers). Management of the horses is quite similar to that of racehorses running on a track. The aim of this study was to determine the prevalence of gastric ulcers found in the glandular or squamous mucosa of 73 animals performing in historical horseraces and determine differences in disease presence and severity between type of competitions, training facilities and breeds of horses. The prevalence of lesions in both mucosae is quite similar to those found in horses performing flat races on a racetrack. Anglo-Arabians used for speed races are more affected than Thoroughbreds, probably because they are involved in a higher number of races and longer traveling times, which are all risk factors for lesions of the squamous and glandular mucosa. These findings highlight the need to implement therapeutic and prophylactic protocols against gastric ulcers also in horses involved in historical horseracing: feedings with a reduced amount of starch, hay nets to increase the amount of time spent eating, paddock turnout for most of the day and the use of supplements to support gastric health are effective measures to prevent the development of gastric ulcers. The use of omeprazole at therapeutic (4 mg/kg per os once daily) or prophylactic (1 mg/kg per os once daily) dosages can also be effective in periods of high stress, especially when horses travel for longer distance or are exercised or raced more. Further studies are needed in this population of animals to determine the differences and similarities with horses involved in racing on a track. Equine Gastric Ulcers Syndrome (EGUS) is a worldwide disease present in equids of different breeds, activity levels, and age groups. It is divided into two different illnesses: Equine Squamous Gastric Disease (ESGD) affecting the squamous mucosa and Equine Glandular Gastric Disease (EGGD) affecting the glandular mucosa. The historical horserace is a traditional competition that is common in Italy. They can be divided into two different types: speed races and jousting tournaments. Anglo-Arabians and Thoroughbreds are used for the two competitions with training and management systems similar to those used in races performed on a racetrack. The aim of this study was to determine the prevalence of ESGD and EGGD in horses used for historical horseracing and evaluate the differences in the presence of the diseases in animals of the two different breeds used for the two types of competition. A cohort of 73 horses was enrolled in this study, which were stabled in 10 training facilities and performed two jousting tournaments and one speed race. An ESGD at least of grade 2 was found in 88% of horses with all degrees of severity seen; EGGD was diagnosed in 45% of animals. In this cohort of horses, the presence and severity of ESGD and EGGD are similar to that in reports in racehorses performing on racetracks. Anglo-Arabians used for speed races are more affected by ESGD and EGGD, which is probably because they are involved in a higher number of races and travel more during the year compared to Thoroughbreds used for jousting competitions. [ABSTRACT FROM AUTHOR]

Simple Summary: Radiography is important for the diagnosis of prematurity and musculoskeletal abnormalities in foals. Given that no data on the normal radiographic anatomical development of the mule foal are available, we evaluated the radiographic development of the mule fore digit and carpal joint from 0 to 3 months of age. The radiographs highlighted that growth plate closure times differed depending on the fore digit bones, and on whether the carpal bones were immature at birth. Distal phalanx was triangular at birth, palmar processes had developed by one month, and proximal sesamoid bones were trapezoidal by three months. No distal phalanx remodeling was evident within the hoof capsule or variations in the hoof axis with growth, and hoof capsule thickness increased. We identified a baseline for the interpretation of radiological features of the forelimb in the mule foal, and we highlighted specific radiographic parameters that were intermediate between those found in horses and donkeys. Few studies have established the normal radiographic anatomical development of the donkey foal and, to date, no data are available for mules. Our aim was thus to evaluate the radiographic development of the fore digit and carpal joint in the mule foal from 0 to 3 months of age. Ten forelimbs of five healthy full-term mule foals were included. Radiographs of the fore digit lateromedial and dorsopalmar and the carpus dorsopalmar were performed weekly for the first month of age, and bi-monthly during the following two months. Fore digit growth plate closure times, morphological, angular, and linear radiographic parameters, and also carpal cuboidal bone mineralization were evaluated. Growth plates were graded as open, closing, and closed. Carpal bone appearance was graded as mature, slightly immature, or immature. Growth plate closure times showed the following: middle phalangeal distal physis (DP) closed at birth, and proximal physis (PP) started to close at around two months; first phalanx DP closed at seven days, and PP started to close at three months; and third metacarpal bone DP started to close at two months. Carpal bones were immature at birth. Distal phalanx (PD) was triangular at birth, palmar processes had developed by one month, and proximal sesamoid bones were trapezoidal by three months. The hoof wall, PD dorsal wall, and palmar angles values changed in parallel, and hoof capsule thickness increased. No PD remodeling evidence within the hoof capsule or variations in the hoof axis with growth were found. We identified a baseline for the interpretation of forelimb radiological features in mule foals. The specific features found were intermediate between those found in horses and donkeys. [ABSTRACT FROM AUTHOR]

Simple Summary: Inhalation of alcohol vapors from hand sanitizers or the use of alcohol-based mouthwashes produces a positive breath test for alcohol for a 5–10 min period. Abdominal ultrasonography is a diagnostic tool widely used by equine clinicians and is performed with alcohol saturation. In addition, the total examination time may vary depending on many factors, and consequently, the amount of alcohol used is also variable. The aim of this study was to describe the breath alcohol test results obtained by equine veterinarians performing abdominal ultrasounds on horses. Our hypothesis was that, after the procedure, positive results are obtained after more than 5–10 min, as described for the use of hand sanitizers or mouthwashes. A total of 36 examinations were performed by six operators. The time needed to reach a negative result ranged between 0 and 60 min. The amount of ethanol used ranged between 100 and 2500 mL. A significant difference was found for the group that used more than 1000 mL compared with the groups that used less than 1000 mL. Based on this study, equine veterinarians that attend colic emergencies can test positive at the breath alcohol test for up to 60 min, especially if they use more than 1 L of ethanol. Transcutaneous abdominal ultrasonography using alcohol saturation is used in the diagnostic workup of a variety of conditions in horses. The duration of the examination and the amount of alcohol used in each case may vary depending on several factors. The aim of this study is to describe the breath alcohol test results obtained by veterinarians performing abdominal ultrasound on horses. Six volunteers were enrolled, after written consent, and a Standardbred mare was used for the whole study protocol. Each operator performed a total of 6 ultrasounds by pouring the ethanol solution from a jar or by spray application, for a duration of 10, 30, and 60 min. An infrared breath alcohol analyzer was used immediately after completing the ultrasonography and at 5-min intervals until a negative result was obtained. Positive results were obtained for 0–60 min after the procedure. A statistically significant difference was found between the groups that used more than 1000 mL, 300–1000 mL, and less than 300 mL of ethanol. No significant differences were observed between the type of ethanol administration and the time of exposure. Based on this study, equine vets who perform ultrasound on horses can test positive at the breath alcohol test for up to 60 min following ethanol exposure. [ABSTRACT FROM AUTHOR]

Simple Summary: The ultrasound appearance of cartilage thickness and subchondral bone changes according to the age of the horse and the health status of the joints. Specific ultrasound features have been described in different joint diseases in horses. Knowledge of the normal features is thus essential during clinical investigations. The present research evaluates the ultrasound features of metacarpal/tarsal-phalangeal joints in healthy racehorses, according to the age and breed. Twenty-eight racehorses (standardbreds and thoroughbreds) were enrolled and grouped according to age (<5 and ≥5 years old). Fetlock cartilage appearance, thickness and subchondral bone appearance were ultrasonographically assessed. In younger compared to older standardbreds, the cartilage appeared thinner and with a normal ultrasound appearance, which suggests the use of specific references for the ultrasound features of the fetlock. In adult horses, specific ultrasound (US) features and reference values have been reported for the appearance of the joint cartilage and thickness according to the type of joint, such as femoropatellar and tarsocrural. The US appearance of the fetlock has been described in several diseases. The present research evaluates the US features of the metacarpal/tarsal-phalangeal joints in healthy racehorses according to age and breed, since no information is available in the literature. Seventy-one fetlocks in 28 healthy horses (15/28 thoroughbreds and 13/28 standardbreds) were assessed. The horses were grouped as follows: group A < 5 years old vs. group B ≥ 5. A portable ultrasound machine and a linear transducer (5–7.5 MHz) were used. Dorsal metacarpal/tarsal-phalangeal joints were scanned. The US images were reviewed offline in terms of articular cartilage appearance, thickness, and subchondral bone appearance by an experienced observer. Data were reported as the median, minimum, and maximum for cartilage thickness values, and differences between groups were evaluated. Cartilage thickness values were statistically lower in group A than B in the standardbreds, except for the lateral thickness in longitudinal view. No differences were detected in the thoroughbreds within age groups. All of the young standardbreds showed a normal cartilage and subchondral appearance. No statistical differences were found between breeds. Our results highlight the characteristics of the US appearance of metacarpal/tarsal-phalangeal joints specifically in racehorses, with some variations according to age. Since the cartilage can change according to joint growth, age and training activity, the present findings suggest the use of specific references for US features, which are key to correctly evaluating the health of the fetlock. [ABSTRACT FROM AUTHOR]

Simple Summary: Ultrasonography is an accurate method to objectively measure subcutaneous fat (SF) thickness and to routinely predict body fat reserves in cows, horses, and donkeys. The aim of the present study was to describe ultrasonographic SF thickness in periparturient jennies. The SF was evaluated in 6 different truncal location in 6 dairy jennies prior and post parturition. Measurement values were reported and each site were compared through time. No statistically significant differences were found between sites and time. The mean values of SF measurements were above those reported by others. A good and reliable association was detected between body condition score (BCS) and sites during the whole study period. Our study gives a preliminary description of different body locations fat reserves evaluated by ultrasonography, showing no significative variations, in pregnant and lactating jennies. The body condition score (BCS) represents a practical but subjective method for assessing body fat reserves. Real time ultrasonography (RTU) has been proposed as an accurate method to objectively measure subcutaneous fat (SF) thickness and predict body fat reserves in cows, horses and donkeys. The aim of the present study was to describe RTU measures of SF thickness during periparturient period in jennies. The present prospective cohort study evaluated six dairy jennies. SF RTU were performed at 15 and 7 days before the presumptive delivery, and 2, 15 and 30 days after delivery. A portable ultrasound machine and multifrequency linear transducer (5–7.5 MHz) was used. RTU images were obtained in six sites (S1–S6). Results at each time point were reported as mean ± standard deviation and compared through time. A total of 180 images were evaluated. RTU technique was easy to perform and well tolerated. No statistically significant differences were found of each site during time, except for S2 and S6a: S2 at T2 and S6a at T1 were significatively different to values obtained at T5. The RTU mean values were above those reported by others, suggesting major physio-logical challenges related to energy balance and fat mobilization in pregnant jennies bred for milking production. BCS and sites through observational time have shown a good and reliable association. Our study could give preliminary indications on fat reserves in different body locations evaluated thanks to RTU and it show no significative variation of SF thickness, in pregnant and lactating jennies. [ABSTRACT FROM AUTHOR]

Simple Summary: An association between increased metabolic demands and reduced dry matter intake is observed from late gestation to early lactation in donkeys. Furthermore, little is known about the nutritional and energy requirements of this period in animals. Changes in energy metabolism make donkeys more susceptible to metabolic diseases such as hyperlipemia, which is characterized by the mobilization of fatty acids from adipose tissue. A better knowledge of this period could improve animal husbandry, well-being, and health. The aim of this study was to analyze the plasma total lipid fraction, to highlight metabolic changes from the pre-foaling to post-foaling periods, using the gas chromatography technique. Our findings reveal a greater risk of metabolic disease in late gestation to early lactation in donkeys. The period from late gestation to early lactation is characterized by changes in energy metabolism. The aim of the current study was to analyze the plasma total lipid fraction using gas chromatography (GC) analysis, in order to highlight metabolic changes from the pre-foaling to post-foaling periods. Eleven pluriparous dairy jennies (mean age of 11.88 ± 3.79 years) belonging to the Amiata donkey breed were enrolled. Blood sampling was performed at 15 days before foaling (T0), and 15 (T1), 30 (T2), 60 (T3), and 90 (T4) days after foaling, for biochemical and GC analysis. A total of 37 fatty acids were identified in plasma samples: 4 medium chain (MCFA), 24 long chain (LCFA), and 9 very-long chain (VLCFA) fatty acids. Among them, 20 fatty acids changed significantly, and two fatty acid showed a trend toward significance. Furthermore, the LCFA, saturated, unsaturated, monounsaturated, and polyunsaturated ω-3 fatty acids changed significantly during the study period. The main alterations were between T0 and the other time points and appeared to be related to lipid metabolism, cellular structure and function, and inflammatory and immune responses. Our findings reveal greater energy requirements at the end of gestation compared to early lactation in donkeys. [ABSTRACT FROM AUTHOR]

Simple Summary: Animal-vehicle collisions are the major cause of rescue and need for hospitalization in wildlife referral centers. Radiography is used to assess the traumatized animal and is a rapid means to evaluate various traumatic pathologies. Clinicians can exploit radiography when making rapid decisions about whether to euthanize or treat an animal. We evaluated data (reasons for rescue, diagnosed lesions, and outcome) from a population of hospitalized wildlife ungulates and we investigated the benefits of using radiography as a diagnostic tool. Animal-vehicle collisions are the major cause of rescue and need for hospitalization in wildlife referral centers. Clinicians need easy-to-use tools to make rapid decisions about whether to euthanize or treat injured animals. The aim of the study was to evaluate the data (reasons for rescue, diagnosed lesions, and outcome) from a hospitalized population of wildlife ungulates and investigate the benefits of using radiography as a diagnostic tool. Data were collected from three wildlife referral centers in Tuscany (Italy). The following information was collected for each animal: reason for hospitalization, clinical examination, radiographic examination, definitive diagnosis, and outcome. A chi-squared test was used to assess the benefits of radiography in detecting different traumatic lesions. Prevalence was reported according to the reason for hospitalization, definitive diagnosis, radiographic diagnosis, and outcome. The main reason for hospitalization was traumatic lesions due to vehicle collisions and 71.1% of the animals did not survive. Radiography was more useful in patients with traumatic axial skeletal lesions and/or multiple traumas with respect to traumatic appendicular skeleton lesions. Our results show that radiography is a useful diagnostic technique for assessing wildlife emergencies and it could help the clinician in making medical decisions. [ABSTRACT FROM AUTHOR]

Simple Summary: Procalcitonin (PCT) increased in the case of systemic inflammatory response syndrome (SIRS), especially due to bacterial infection. The correlation between SIRS score and plasma PCT levels in horses have not been evaluated, and no studies investigated plasma PCT concentration over time. In the present study, PCT and SIRS score were evaluated in colic horses at admission to the hospital and at 24, 48, 72 and 96 h. Statistically differences were detected between healthy vs. all colic horses and between healthy vs. SIRS positive or SIRS negative horses. No correlation was observed between SIRS score and PCT. This suggests a role of plasmatic PCT as good biomarker for colic. Colic horses show systemic inflammatory response syndrome (SIRS) clinical signs. Procalcitonin (PCT) showed increased circulating levels in sick horses. This study compares plasma PCT concentrations in healthy vs. SIRS negative/positive colic horses over time, and evaluates PCT and SIRS score potential correlation, to verify the usefulness of PCT for the evaluation of SIRS severity. Ninety-one horses were included; 43/91 were healthy, on basis of physical examination, blood work and SIRS score (score = 0), while 48/91 were sick colic horses, classified as SIRS-negative (score < 2) and positive (score ≥ 2). Moreover, a 0–6 point-scale SIRS score was calculated (assessing mucous membrane color and blood lactate concentration). PCT was evaluated at admission, and at 24, 48, 72 and 96 h, using a commercial kit for equine species. We verified by the ANOVA test PCT differences between healthy vs. colic horses, healthy vs. SIRS-negative or SIRS-positive colic horses, at all sampling times, and the correlation between the SIRS score at admission with the SIRS score. Statistically significant differences were detected between healthy vs. all colic horses and between healthy vs. SIRS-positive or negative horses at all sampling times. No correlation was observed between the SIRS score at admission and PCT values. PCT was statistically higher in colic horses compared to the healthy ones, suggesting a role as a biomarker for colic. [ABSTRACT FROM AUTHOR]

Simple Summary: Umbilicus assessment is an essential part of the clinical examination in neonates because the umbilicus could be a potential access-point for pathogens. Thus, an early and accurate diagnosis is pivotal in order to check for any evidence of umbilical infections and potentially life-threating sepsis. Ultrasound scanning has been shown to be a valuable tool, both in equine foals and calves, in order to assess the diameter and appearance of external and intra-abdominal umbilicus remnant. To the best of our knowledge, no reports are available for donkey foals; thus, the present study evaluates ultrasonography and measures the umbilical remnants in donkey foals in the first week of life. No statistical differences in the size and appearance of the umbilical remnants were observed during the first week of life in the donkey foals, and the measurement values reported in this study are comparable with the ones obtained in equine foals and calves. The umbilicus is a potential access-point for pathogens in equine foal, causing umbilical infections and potentially life-threating illness. Early diagnosis based on ultrasonographic appearance and measurement is crucial to avoid severe complications and promptly implement appropriate therapy. This study ultrasonographically evaluates the umbilical remnants of donkey foals, in the first week of life. Fifteen healthy donkey foals were included in the study. The umbilical vein, arteries and urachus ultrasounds were performed at 24 h, 3 and 7 days of life, using a portable ultrasound machine and a 5–7.5 MHz multifrequency linear probe. The Kruskal–Wallis test and Dunn's multiple comparisons test were applied to verify differences in relation to time for all the umbilical remnants measured. Statistical significance was set at p < 0.05. No statistical differences were observed in relation to time regarding umbilical remnant measurements. A correlation was found between body weight and the left artery at T0. The regression of the umbilical remnant during the first week of life was slower compared with what was reported in equine foals but was comparable with the results on calves. Thus, the different regression timing needs be considered when evaluating donkey foals with umbilical remnant diseases within the first week of life. [ABSTRACT FROM AUTHOR]

Simple Summary: Horse hoof possesses a micro-flora that may promote the development of secondary infections following wounds or surgery. For this reason, in vitro and in vivo experiments were used to verify and compare the bactericidal power of two well-known disinfectants, Povidone-iodine (PI) and Chlorhexidine (CHx), and a new stable colloidal suspension of iron oxide, called Iron Animals (IA). In vitro, IA was able to completely suppress the bacterial growth on all of the tested microorganisms. In vivo, PI alone possessed the lowest efficacy for hoof disinfection; CHx resulted as the best treatment in 18% of cases. CHx + IA emerged as the best disinfection protocol for equine hoof achieving the lowest bacterial load in 55% of cases. The addition of IA, after PI or CHx, improved both disinfectants' effectiveness, leading to the highest bactericidal activity in 82% of cases. IA would deserve a chance as a possible solution in pre-surgical disinfection of the equine hoof, reducing the risk of infection. The presence of bacteria of various origins on horse hoofs enables the onset of infections following trauma or even post-surgical wounds. Thus, the analysis of new antibacterial substances is of fundamental importance. In this study, the antibacterial efficacy of Iron Animals (IA), a stable colloidal suspension of iron oxide, organic acids, and detergents, was tested in vitro and in vivo. In vitro assays were performed to test the unspecific inhibitory effect of IA on both gram-positive and gram-negative bacteria monitoring the microorganism growth by spectrophotometry (optical density OD600) at 37 °C for 24 h. In vivo test consists on the quantification of the bacterial load in colony forming units per gram (CFU/g) of specimens collected from the frog region of the anterior hooves of 11 horses. Sampling followed the application of four disinfectant protocols consisting of two consecutive 3 min scrubs with 50 mL of 10% Povidone-iodine (PI) or 4% Chlorhexidine (CHx), with or without an additional application for 15 min of 10 mL of Iron Animals (PI+IA and CHx+IA). In vitro, IA completely suppressed the bacterial growth of all the tested microorganisms, resulting in effectiveness also against CHx-resistant bacteria, such as Staphylococcus aureus. In vivo, PI emerged as an ineffective protocol; CHx was effective in 18% of cases, but with the addition of IA (CHx + IA) its use emerged as the best disinfectant protocol for horse hoof, achieving the lowest bacterial load in 55% of cases. The addition of IA, after PI or CHx, improves the effectiveness of both disinfectants leading to the highest bactericidal activity in 82% of cases. [ABSTRACT FROM AUTHOR]

Simple Summary: Little is known about the passive transfer of immunity in donkey foals and about the different types of analysis that can be performed to assess it. The aims of the present study were to evaluate the correlation between IgG Serum Radial Immunodiffusion, Electrophoresis Gamma Globulins, Electrophoresis Total Protein and the Serum Total Protein analyzed by Refractometry and by Dry Chemistry Analyzer (Biuret) and to estimate serum IgG concentrations using Serum TP. IgG Serum Radial Immunodiffusion showed a good correlation with Electrophoresis Gamma Globulins and a high correlation with Total Protein Electrophoresis, Biuret and Refractometry. All the tests performed may be a useful to estimate the serum IgG in donkey foals' blood in the first day of life using a specific equation. The aims of the present study were to evaluate the correlation between IgG Serum Radial Immunodiffusion (SRID), Electrophoresis Gamma Globulins (EGG), Electrophoresis Total Protein (ETP) and the serum total protein (TP) analyzed by refractometry and by a dry chemistry analyzer (Biuret) and to estimate serum IgG concentrations using serum TP. A total of 36 samples collected at four different times (birth, 6, 12, 24 h after birth) from nine Amiata donkey foals were evaluated with SRID, EGG, ETP, serum TP Biuret and refractometry. SRID IgG concentration increased significantly over time until T12. Serum TP analyzed with refractometry, electrophoresis and Biuret showed a statistically significant difference between T0 and T6 vs. T12 and T24. A good or strong correlation was found between different tests performed. Equations to quantify serum IgG were created and can be used for estimating the donkey foals' serum IgG in the first day of life. Serum TP refractometry showed a high correlation with SRID IgG (0.91) which may be a particularly useful and economic instrument to estimate the transfer of immunity in donkey foals during the first day of life. Further studies evaluating a high number of animals are needed in order to set specific cut-off values. [ABSTRACT FROM AUTHOR]