1. Complete Genomic Sequence of Bacteriophage Felix O1.
- Author
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Whichard, Jean M., Weigt, Lee A., Borris, Douglas J., Ling Ling Li, Qing Zhang, Kapur, Vivek, Pierson, F. William, Lingohr, Erika J., Yi-Min She, Kropinski, Andrew M., and Sriranganathan, Nammalwar
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SALMONELLA detection ,BACTERIOPHAGES ,NUCLEOTIDE sequence ,BIOINFORMATICS ,TRANSFER RNA ,ERWINIA amylovora ,ESCHERICHIA coli ,FOODBORNE diseases ,GENOMICS - Abstract
Bacteriophage O1 is a Myoviridae A1 group member used historically for identifying Salmonella. Sequencing revealed a single, linear, 86,155-base-pair genome with 39% average G+C content, 131 open reading frames, and 22 tRNAs. Closest protein homologs occur in Erwinia amylovora phage φEa21-4 and Escherichia coli phage wV8. Proteomic analysis indentified structural proteins: Gp23, Gp36 (major tail protein), Gp49, Gp53, Gp54, Gp55, Gp57, Gp58 (major capsid protein), Gp59, Gp63, Gp64, Gp67, Gp68, Gp69, Gp73, Gp74 and Gp77 (tail fiber). Based on phage-host codon differences, 7 tRNAs could affect translation rate during infection. Introns, holin-lysin cassettes, bacterial toxin homologs and host RNA polymerase-modifying genes were absent. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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