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Simple Summary: Although scientific evidence for treatment efficacy is lacking, transcutaneous electrical nerve stimulation is used in dogs as a pain-relieving treatment. This randomised single-blinded cross-over study aims to investigate whether treatment with transcutaneous electrical nerve stimulation will affect gait parameters in dogs with osteoarthritis. Fifteen dogs were included in the study, and all dogs were over one year of age, lame, and had chronic pain for more than three months. The dogs were treated with transcutaneous electrical nerve stimulation for seven or ten days, and their gait pattern in trot was evaluated with a pressure-sensitive mat. In the present study, no significant differences were seen between transcutaneous electrical nerve stimulation and placebo treatments for any of the gait parameters evaluated by the pressure-sensitive mat. Further studies are needed to confirm the observations. Osteoarthritis is a common degenerative disease in dogs, often manifested as pain, joint swelling, and lameness. Despite the lack of scientific evidence for its treatment efficacy, transcutaneous electrical nerve stimulation (TENS) is used in dogs as a pain-relieving treatment. This randomised single-blinded cross-over study investigated the effect of TENS on gait parameters in fifteen dogs with osteoarthritis. Stance time, swing time, stride time, stride length, peak vertical force (%BW), vertical impulse (%BW*sec), and symmetry indices were obtained using a pressure-sensitive mat. TENS treatment of 80 Hz and 100 µs with an individually selected amplitude was conducted for 45 min once daily for a treatment period of seven or ten days. No significant differences were seen between TENS and placebo for any of the gait parameters. Hence, in this study, TENS did not affect gait parameters, compared to placebo. Further studies are needed to confirm the observations. [ABSTRACT FROM AUTHOR]

Simple Summary: Lameness, an alteration of the gait due to pain or dysfunction of the locomotor system, is the most common disease symptom in horses. Yet, it is difficult for veterinarians to correctly assess by visual inspection. Objective tools that can aid clinical decision making and provide early disease detection through sensitive lameness measurements are needed. In this study, we describe how an AI-powered measurement tool on a smartphone can detect lameness in horses without the need to mount equipment on the horse. We compare it to a state-of-the-art multi-camera motion capture system by simultaneous, synchronised recordings from both systems. The mean difference between the systems' output of lameness metrics was below 2.2 mm. Therefore, we conclude that the smartphone measurement tool can detect lameness at relevant levels with easy-of-use for the veterinarian. Computer vision is a subcategory of artificial intelligence focused on extraction of information from images and video. It provides a compelling new means for objective orthopaedic gait assessment in horses using accessible hardware, such as a smartphone, for markerless motion analysis. This study aimed to explore the lameness assessment capacity of a smartphone single camera (SC) markerless computer vision application by comparing measurements of the vertical motion of the head and pelvis to an optical motion capture multi-camera (MC) system using skin attached reflective markers. Twenty-five horses were recorded with a smartphone (60 Hz) and a 13 camera MC-system (200 Hz) while trotting two times back and forth on a 30 m runway. The smartphone video was processed using artificial neural networks detecting the horse's direction, action and motion of body segments. After filtering, the vertical displacement curves from the head and pelvis were synchronised between systems using cross-correlation. This rendered 655 and 404 matching stride segmented curves for the head and pelvis respectively. From the stride segmented vertical displacement signals, differences between the two minima (MinDiff) and the two maxima (MaxDiff) respectively per stride were compared between the systems. Trial mean difference between systems was 2.2 mm (range 0.0–8.7 mm) for head and 2.2 mm (range 0.0–6.5 mm) for pelvis. Within-trial standard deviations ranged between 3.1–28.1 mm for MC and between 3.6–26.2 mm for SC. The ease of use and good agreement with MC indicate that the SC application is a promising tool for detecting clinically relevant levels of asymmetry in horses, enabling frequent and convenient gait monitoring over time. [ABSTRACT FROM AUTHOR]

Simple Summary: Pain scales are tools developed to improve pain assessment in horses. They are based on behaviors and/or facial expressions, and the observer allocates a score based on the character of the behavior or facial expression. Little is known about behaviors and facial expressions at rest in horses with orthopedic pain since pain is mainly assessed by lameness evaluation during movement. The aim of this study was to describe how closely equine behaviors and facial expressions are associated with movement asymmetry and to identify combinations of behavior and expressions present in horses with induced orthopedic pain. Orthopedic pain was induced in eight horses and assessed in two ways; using four existing equine pain scales at rest, and by measuring movement asymmetry during movement. The association of behavior and facial expression items in the pain scales with actual lameness was analyzed. Posture-related behavior showed the strongest association, while facial expressions varied between horses. These results show that pain scales for orthopedic pain assessment would benefit from including posture, head position, location in the box stall, focus, interactive behavior, and facial expressions. This could improve orthopedic pain detection in horses during rest with mild lameness. Equine orthopedic pain scales are targeted towards horses with moderate to severe orthopedic pain. Improved assessment of pain behavior and pain-related facial expressions at rest may refine orthopedic pain detection for mild lameness grades. Therefore, this study explored pain-related behaviors and facial expressions and sought to identify frequently occurring combinations. Orthopedic pain was induced by intra-articular LPS in eight horses, and objective movement asymmetry analyses were performed before and after induction together with pain assessments at rest. Three observers independently assessed horses in their box stalls, using four equine pain scales simultaneously. Increase in movement asymmetry after induction was used as a proxy for pain. Behaviors and facial expressions commonly co-occurred and were strongly associated with movement asymmetry. Posture-related scale items were the strongest predictors of movement asymmetry. Display of facial expressions at rest varied between horses but, when present, were strongly associated with movement asymmetry. Reliability of facial expression items was lower than reliability of behavioral items. These findings suggest that five body behaviors (posture, head position, location in the box stall, focus, and interactive behavior) should be included in a scale for live assessment of mild orthopedic pain. We also recommend inclusion of facial expressions in pain assessment. [ABSTRACT FROM AUTHOR]

Simple Summary: The influence of rider weight on horse welfare, health and performance is often debated. We measured the effects of increasing the weight of the regular rider by 15% and 25% on horse behavior, gait symmetry and physiological responses in a standard dressage test. Cortisol levels increased in response to exercise, but we found no effect of the weight treatment, i.e., cortisol levels did not increase when the rider became heavier. Behavior, heart rate and gait symmetry also did not differ between treatments. We conclude that increasing the weight of the regular rider by 15% and 25% did not result in significant short-term alterations in cortisol, heart rate, behavior and gait symmetry in horses during low-intensity exercise. Further studies are required to develop appropriate guidelines for rider weight. Recent studies have reported significant alterations in horse physiological and gait parameters when exposed to increased rider weight during moderate to high intensity exercise. This study aimed to evaluate the effect of increased rider weight (+15% and +25% of the regular rider's bodyweight) on horse behavioral, physiological and gait symmetry parameters during a standard dressage test. Twenty rider-horse equipages performed the same test three times in a randomized, crossover design. Salivary cortisol (SC), heart rate (HR), heart rate variability (HRV), behavior and gait symmetry (GS) were measured. SC concentrations increased from baseline (p < 0.001), but there was no significant treatment effect (difference from baseline (ng/mL): Control: 0.21 ± 0.1; +15%: 0.37 ± 0.1; +25%: 0.45 ± 0.2, p = 0.52). Similarly, there were no overall treatment effects on HR or HRV variables (avg HR across treatments (bpm): 105.3 ± 1.3), nor on GS parameters. There was large individual variation in conflict behavior but no effect of weight treatment. We conclude that increasing the weight of the regular rider by 15% and 25% did not result in significant short-term alterations in the measured parameters. Maximum rider:horse weight ratios were 15–23% and the exercise intensity was relatively low; thus the results should not be extrapolated to other weight ratios and exercise intensities. [ABSTRACT FROM AUTHOR]

Simple Summary: Horse management aims to keep horses healthy and ensure good performance and animal welfare. Many horses are currently kept in individual box stalls indoors, a housing system that limits free movement, exploration, and social interaction, and may also subject horses to lower air quality. The alternative is a free-range housing system where horses are kept in groups outdoors. Anecdotal information indicates concerns among sports horse trainers that lack of rest in such systems delays recovery and impairs performance. This study examined whether recovery after competition-like exercise in Standardbred trotters was affected by housing system. The results showed that a free-range housing system did not delay recovery in Standardbred trotters, and in fact had positive effects on appetite and recovery of energy balance. This study examined the effects of two housing systems (free-range and box stalls) on recovery of energy balance after competition-like exercise in Standardbred horses. Eight adult geldings (mean age 11 years) were used. The study had a change-over design, with the box stall (BOX) and free-range group housing (FreeR) treatments each run for 21 days. The horses were fed forage ad libitum and performed two similar race-like exercise tests (ET), on day 7 and day 14 in each treatment. Forage intake was recorded during the last 6–7 days in each period. Blood samples were collected before, during, and until 44 h after ET. Voluntary forage intake (measured in groups with four horses in each group) was higher in FreeR horses than BOX horses (FreeR: 48, BOX: 39, standard error of the mean (SEM) 1.7 kg (p = 0.003)). Plasma non-esterified fatty acids (NEFA) was lower at 20–44 h of recovery than before in FreeR horses (p = 0.022), but not in BOX horses. Housing did not affect exercise heart rate, plasma lactate, plasma urea, or total plasma protein concentration. Thus the free-range housing system hastened recovery in Standardbred trotters, contradicting anecdotal claims that it delays recovery. The free-range housing also had positive effects on appetite and recovery of energy balance. [ABSTRACT FROM AUTHOR]