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Phlebia radiata is a widespread white-rot basidiomycete fungus with significance in diverse biotechnological applications due to its ability to degrade aromatic compounds, xenobiotics, and lignin using an assortment of oxidative enzymes including laccase. In this work, a chemical screen with 480 conditions was conducted to identify chemical inducers of laccase expression in P. radiata. Among the chemicals tested, phenothiazines were observed to induce laccase activity in P. radiata, with promethazine being the strongest laccase inducer of the phenothiazine-derived compounds examined. Secretomes produced by promethazine-treated P. radiata exhibited increased laccase protein abundance, increased enzymatic activity, and an enhanced ability to degrade phenolic model lignin compounds. Transcriptomics analyses revealed that promethazine rapidly induced the expression of genes encoding lignin-degrading enzymes, including laccase and various oxidoreductases, showing that the increased laccase activity was due to increased laccase gene expression. Finally, the generality of promethazine as an inducer of laccases in fungi was demonstrated by showing that promethazine treatment also increased laccase activity in other relevant fungal species with known lignin conversion capabilities including Trametes versicolor and Pleurotus ostreatus. [ABSTRACT FROM AUTHOR]

The range of applications for industrial hemp has consistently increased in various sectors over the years. For example, hemp hurd can be used as a resource to produce biodegradable packaging materials when incorporated into a fungal mycelium composite, a process that has been commercialized. Although these packaging materials can be composted after usage, they may present an opportunity for valorization in a biorefinery setting. Here, we demonstrate the potential of using this type of discarded packaging composite as a feedstock for biofuel production. A one-pot ionic liquid-based biomass deconstruction and conversion process was implemented, and the results from the packaging material were compared with those obtained from untreated hemp hurd. At a 120 °C reaction temperature, 7.5% ionic liquid loading, and 2 h reaction time, the packaging materials showed a higher lignocellulosic sugar yield and sugar concentrations than hemp hurd. Hydrolysates prepared from packaging materials also promoted production of higher titers (1400 mg/L) of the jet-fuel precursor bisabolene when used to cultivate an engineered strain of the yeast Rhodosporidium toruloides. Box–Behnken experiments revealed that pretreatment parameters affected the hemp hurd and packaging materials differently, evidencing different degrees of recalcitrance. This study demonstrated that a hemp hurd-based packaging material can be valorized a second time once it reaches the end of its primary use by supplying it as a feedstock to produce biofuels. [ABSTRACT FROM AUTHOR]

In this work, a real-time software-in-loop simulation technique was employed to test and analyse an underwater wireless sensor network. This simulation should facilitate the deployment of the real network and helps guarantee the network's expected behaviour. We study duplicated packets, one-way delay, and power consumption to analyse the network's leading parameters. Evaluating production-ready software in simulated conditions eases effective deployment. This method will ultimately allow us to establish these parameters, test the software before the deployment, and have an excellent understanding of the network's behaviour. [ABSTRACT FROM AUTHOR]

Switching losses of power transistors usually are the most relevant energy losses in high-frequency power converters. Soft-switching techniques allow a reduction of these losses, but even under soft-switching conditions, these losses can be significant, especially at light load and very high switching frequency. In this paper, hysteresis and energy losses are shown during the charge and discharge of the output capacitance (COSS) of commercial high voltage Superjunction MOSFETs. Moreover, a simple methodology to include information about these two phenomena in datasheets using a commercial system is suggested to manufacturers. Simulation models including COSS hysteresis and a figure of merit considering these intrinsic energy losses are also proposed. Simulation and experimental measurements using an LLC resonant converter have been performed to validate the proposed mechanism and the usefulness of the proposed simulation models. [ABSTRACT FROM AUTHOR]

Recent studies have found increases in the cardiovascular mortality rates during poor air quality events due to outbreaks of desert dust. In Tenerife, we collected (2014–2017) data in 829 patients admitted with a heart failure diagnosis in the Emergency Department of the University Hospital of the Canaries. In this region, concentrations of PM10 and PM2.5 are usually low (~20 and 10 µg/m3), but they increase to 360 and 115 μg/m3, respectively, during Saharan dust events. By using statistical tools (including multivariable logistic regressions), we compared in-hospital mortality of patients with heart failure and exposure to PM10 and PM2.5 during dust and no-dust events. We found that 86% of in-hospital heart failure mortality cases occurred during Saharan dust episodes that resulted in PM10 > 50 µg/m3 (interquartile range: 71–96 µg/m3). A multivariate analysis showed that, after adjusting for other covariates, exposure to Saharan dust events associated with PM10 > 50 µg/m3 was an independent predictor of heart failure in-hospital mortality (OR = 2.79, 95% CI (1.066–7.332), p = 0.03). In conclusion, this study demonstrates that exposure to high Saharan dust concentrations is independently associated with in-hospital mortality in patients with heart failure. [ABSTRACT FROM AUTHOR]

Cardiovascular disease is the cause of physical infirmity and thousands of deaths annually. Typically, during heart failure, cardiomyocyte mitochondria falter in terms of energy production and metabolic processing. Additionally, inflammation and the accumulation of non-contractile fibrous tissue contribute to cardiac malfunction. Melatonin, an endogenously produced molecule, experimentally reduces the initiation and progression of atherosclerotic lesions, which are often the basis of coronary artery disease. The current review critically analyzes published data related to the experimental use of melatonin to forestall coronary artery pathologies. Collectively, these studies document melatonin's anti-atherosclerotic actions in reducing LDL oxidation and triglyceride levels, lowering endothelial malfunction, limiting adhesion molecule formation, preventing macrophage polarization to the M1 pro-inflammatory phenotype, changing cellular metabolism, scavenging destructive reactive oxygen species, preventing the proliferation and invasion of arterial smooth muscle cells into the lesioned area, restricting the ingrowth of blood vessels from the vasa vasorum, and solidifying the plaque cap to reduce the chance of its rupture. Diabetic hyperglycemia, which aggravates atherosclerotic plaque formation, is also inhibited by melatonin supplementation in experimental animals. The potential value of non-toxic melatonin as a possible inhibitor of cardiac pathology in humans should be seriously considered by performing clinical trials using this multifunctional molecule. [ABSTRACT FROM AUTHOR]

Missing traces in ground penetrating radar (GPR) B-scans (radargrams) may appear because of limited scanning resolution, failures during the acquisition process or the lack of accessibility to some areas under test. Four statistical interpolation methods for recovering these missing traces are compared in this paper: Kriging, Wiener structures, Splines and the expectation assuming an independent component analyzers mixture model (E-ICAMM). Kriging is an adaptation to the spatial context of the linear least mean squared error estimator. Wiener structures improve the linear estimator by including a nonlinear scalar function. Splines are a commonly used method to interpolate GPR traces. This consists of piecewise-defined polynomial curves that are smooth at the connections (or knots) between pieces. E-ICAMM is a new method proposed in this paper. E-ICAMM consists of computing the optimum nonlinear estimator (the conditional mean) assuming a non-Gaussian mixture model for the joint probability density in the observation space. The proposed methods were tested on a set of simulated data and a set of real data, and four performance indicators were computed. Real data were obtained by GPR inspection of two replicas of historical walls. Results show the superiority of E-ICAMM in comparison with the other three methods in the application of reconstructing incomplete B-scans. [ABSTRACT FROM AUTHOR]