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Accurate identification and segmentation of individual tree points are crucial for assessing forest spatial distribution, understanding tree growth and structure, and managing forest resources. Traditional methods based on Canopy Height Models (CHM) are simple yet prone to over- and/or under-segmentation. To deal with this problem, this paper introduces a novel approach that combines marker-controlled watershed segmentation with a spectral clustering algorithm. Initially, we determined the local maxima within a series of variable windows according to the lower bound of the prediction interval of the regression equation between tree crown radius and tree height to preliminarily segment individual trees. Subsequently, using this geometric shape analysis method, the under-segmented trees were identified. For these trees, vertical tree crown profile analysis was performed in multiple directions to detect potential treetops which were then considered as inputs for spectral clustering optimization. Our experiments across six plots showed that our method markedly surpasses traditional approaches, achieving an average Recall of 0.854, a Precision of 0.937, and an F1-score of 0.892. [ABSTRACT FROM AUTHOR]

This paper proposes an encoding–decoding network with a pyramidal representation module, which will be referred to as EDPNet, and is designed for efficient semantic image segmentation. On the one hand, during the encoding process of the proposed EDPNet, the enhancement of the Xception network, i.e., Xception+ is employed as a backbone to learn the discriminative feature maps. The obtained discriminative features are then fed into the pyramidal representation module, from which the context-augmented features are learned and optimized by leveraging a multi-level feature representation and aggregation process. On the other hand, during the image restoration decoding process, the encoded semantic-rich features are progressively recovered with the assistance of a simplified skip connection mechanism, which performs channel concatenation between high-level encoded features with rich semantic information and low-level features with spatial detail information. The proposed hybrid representation employing the proposed encoding–decoding and pyramidal structures has a global-aware perception and captures fine-grained contours of various geographical objects very well with high computational efficiency. The performance of the proposed EDPNet has been compared against PSPNet, DeepLabv3, and U-Net, employing four benchmark datasets, namely eTRIMS, Cityscapes, PASCAL VOC2012, and CamVid. EDPNet acquired the highest accuracy of 83.6% and 73.8% mIoUs on eTRIMS and PASCAL VOC2012 datasets, while its accuracy on the other two datasets was comparable to that of PSPNet, DeepLabv3, and U-Net models. EDPNet achieved the highest efficiency among the compared models on all datasets. [ABSTRACT FROM AUTHOR]

Global Navigation Satellite System interferometric reflectometry (GNSS-IR), a new and inexpensive technique, has become available to the broader scientific community for detecting surface environmental information, such as soil moisture, snow depth and vegetation growth. However, there have been limited experiments focusing on the potential of crop height retrieval, especially the performance evaluation of BeiDou Navigation Satellite System (BDS) with other GNSS. Accuracy and reliability are challenging to achieve with traditional methods utilizing a single GNSS, and few measured verification data. In this study, an improved method that includes segmentation processing and multi-GNSS fusion is proposed based on GPS/GLONASS/Galileo/BDS multi-frequency data. Furthermore, experiments were carried out on a farmland in Fengqiu County, Henan Province, China. The results show that the height retrievals from four GNSS were in good agreement with the in situ observations during the whole growth cycle of the wheat after overwintering. Meanwhile, the retrievals based on the proposed method exhibited greater correspondence than the single frequency results, the correlation coefficient was increased and the root-mean-square error (RMSE) was reduced, respectively. Therefore, this study illustrates the feasibility of the proposed method to precisely estimate wheat height and its potential for use in the early warning of wheat lodging based on GNSS-IR. [ABSTRACT FROM AUTHOR]

Soil moisture is an important geophysical parameter for studying terrestrial water and energy cycles. It has been proven that Global Navigation Satellite System Interferometry Reflectometry (GNSS-IR) can be applied to monitor soil moisture. Unlike the Global Positioning System (GPS) that has only medium earth orbit (MEO) satellites, the Beidou Navigation Satellite System (BDS) also has geosynchronous earth orbit (GEO) satellites and inclined geosynchronous satellite orbit (IGSO) satellites. Benefiting from the distribution of three different orbits, the BDS has better coverage in Asia than other satellite systems. Previous retrieval methods that have been confirmed on GPS cannot be directly applied to BDS MEO satellites due to different satellite orbits. The contribution of this study is a proposed multi-satellite soil moisture retrieval method for BDS MEO and IGSO satellites based on signal-to-noise ratio (SNR) observations. The method weakened the influence of environmental differences in different directions by considering satellite repeat period. A 30-day observation experiment was conducted in Fengqiu County, China and was used for verification. The satellite data collected were divided according to the satellite repeat period, and ensured the response data moved in the same direction. The experimental results showed that the BDS IGSO and MEO soil moisture estimation results had good correlations with the in situ soil moisture fluctuations. The BDS MEO B1I estimation results had the best performance; the estimation accuracy in terms of correlation coefficient was 0.9824, root mean square error (RMSE) was 0.0056 cm3cm−3, and mean absolute error (MAE) was 0.0040 cm3cm−3. The estimations of the BDS MEO B1I, MEO B2I, and IGSO B2I performed better than the GPS L1 and L2 estimations. For the BDS IGSO satellites, the B1I signal was more suitable for soil moisture retrieval than the B2I signal; the correlation coefficient was increased by 19.84%, RMSE was decreased by 42.64%, and MAE was decreased by 43.93%. In addition, the BDS MEO satellites could effectively capture sudden rainfall events. [ABSTRACT FROM AUTHOR]

Picea mongolica is a rare tree species in China, which is of great significance in combating desertification and improving the harsh ecological environment. Due to the low rate of natural regeneration, high mortality, and susceptibility to pests and cold springs, Picea mongolica has gradually become extinct. At present, somatic embryogenesis (SE) is the most effective method of micro-proliferation in conifers, but the induction rate of embryogenic callus (EC) is low, and EC is difficult to differentiate from non-embryonic callus (NEC). Therefore, the EC and NEC of Picea mongolica were compared from the morphology, histological, physiological, and transcriptional levels, respectively. Morphological observation showed that the EC was white and transparent filamentous, while the NEC was compact and brownish-brown lumpy. Histological analyses showed that the NEC cells were large and loosely arranged; the nuclei attached to the edge of the cells were small; the cytoplasm was low; and the cell gap was large and irregular. In the EC, small cells, closely arranged cells, and a large nucleus and nucleolus were observed. Physiological studies showed significant differences in ROS-scavenging enzymes between the EC and NEC. Transcriptome profiling revealed that 13,267 differentially expressed genes (DEGs) were identified, 3682 were up-regulated, and 9585 were down-regulated. In total, 63 GO terms had significant enrichment, 32 DEGs in plant hormone signal transduction pathway were identified, and 502 different transcription factors (TFs) were characterized into 38 TF families. Meanwhile, we identified significant gene expression trends associated with somatic embryo development in plant hormones ( AUX / IAA , YUCCA , LEA , etc.), stress ( GST , HSP , GLP , etc.), phenylpropanoid metabolism ( 4CL , HCT , PAL , etc.), and transcription factors ( AP2 / ERF , MYB, WOX , etc.). In addition, nine genes were chosen for RT-qPCR, and the results were consistent with RNA-Seq data. This study revealed the changes in morphology, histology, physiology, and gene expression in the differentiation of NEC into EC and laid the foundation for finding the key genes to promote EC formation.