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With the development of new technologies in recent years, researchers have made significant progress in crop breeding. Modern breeding differs from traditional breeding because of great changes in technical means and breeding concepts. Whereas traditional breeding initially focused on high yields, modern breeding focuses on breeding orientations based on different crops' audiences or by-products. The process of modern breeding starts from the creation of material populations, which can be constructed by natural mutagenesis, chemical mutagenesis, physical mutagenesis transfer DNA (T-DNA), Tos17 (endogenous retrotransposon), etc. Then, gene function can be mined through QTL mapping, Bulked-segregant analysis (BSA), Genome-wide association studies (GWASs), RNA interference (RNAi), and gene editing. Then, at the transcriptional, post-transcriptional, and translational levels, the functions of genes are described in terms of post-translational aspects. This article mainly discusses the application of the above modern scientific and technological methods of breeding and the advantages and limitations of crop breeding and diversity. In particular, the development of gene editing technology has contributed to modern breeding research. [ABSTRACT FROM AUTHOR]

To enhance the performance of tubular microbubble generators, the Volume of Fluid (VOF) multiphase flow model in COMSOL Multiphysics was used to simulate the bubble fragmentation characteristics within a throttling hole microbubble generator. The effects of the inlet speed of the throttling hole pipe, the diameter of the throttling hole, and the length of the expansion section on bubble fragmentation performance were analyzed. The results indicated that an increase in the inlet speed of the throttling hole pipe gradually improved the bubble fragmentation performance. However, an increase in the throttling hole diameter significantly reduced the bubble fragmentation performance. Changes in the length of the expansion section had a minor impact on the bubble fragmentation performance. Experimental methods were used to verify the characteristics of bubble fragmentation, and it was found that the simulation and experimental results were consistent. This provides a theoretical basis and practical guidance for the design optimization of tubular microbubble generators. [ABSTRACT FROM AUTHOR]

Aqueous Zn-ion Batteries (AZIBs) have garnered significant interest in recent years, owing to their inherent safety, affordability, and eco-friendliness. Recently, substantial research has been conducted to broaden the application scenarios of AZIBs by regulating the electrode and electrolyte materials. In this review, we provide a comprehensive analysis of the challenges and solutions associated with AZIBs to meet extreme conditions, such as low temperatures, high temperatures, and wide temperature ranges. We also discuss electrolyte optimization strategies for each of these conditions. Finally, we outline potential avenues for further advancements and offer insights into the future of this burgeoning field of AZIBs. [ABSTRACT FROM AUTHOR]

Periodic noise is a well-known problem in seismic exploration, caused by power lines, pump jacks, engine operation, or other interferences. It contaminates seismic data and affects subsequent processing and interpretation. The conventional methods to attenuate periodic noise are notch filtering and some model-based methods. However, these methods either simultaneously attenuate noise and seismic events around the same frequencies, or need expensive computation time. In this work, a new method is proposed to attenuate periodic noise based on sparse representation. We use a noise dictionary to sparsely represent periodic noise. The noise dictionary is constructed based on ambient noise. An advantage of our method is that it can automatically suppress monochromatic periodic noise, multitoned periodic noise and even periodic noise with complex waveforms without pre-known noise frequencies. In addition, the method does not result in any notches in the spectrum. Synthetic and field examples demonstrate that our method can effectively subtract periodic noise from raw seismic data without damaging the useful seismic signal. [ABSTRACT FROM AUTHOR]

Under the assumptions of linear elasticity and small deformation in traditional elastodynamics, the anisotropy of the medium has a significant effect on rotations observed during earthquakes. Based on the basic theory of the first-order velocity-stress elastic wave equation, this paper simulates the seismic wave propagation of the translational and rotational motions in two-dimensional isotropic and VTI (transverse isotropic media with a vertical axis of symmetry) media under different source mechanisms with the staggered-grid finite-difference method with respect to nine different seismological models. Through comparing the similarities and differences between the translational and rotational components of the wave fields, this paper focuses on the influence of anisotropic parameters on the amplitude and phase characteristics of the rotations. We verify that the energy of S waves in the rotational components is significantly stronger than that of P waves, and the response of rotations to the anisotropic parameters is more sensitive. There is more abundant information in the high-frequency band of the rotational components. With the increase of Thomsen anisotropic parameters ε and δ, the energy of the rotations increases gradually, which means that the rotational component observation may be helpful to the study of anisotropic parameters. [ABSTRACT FROM AUTHOR]

Rotational observation is essential for a comprehensive description of the ground motion, and can provide additional wave-field information. With respect to the three typical layered models in shallow engineering geology, under the assumption of linear small deformation, we simulate the 2-dimensional radial, vertical, and rotational components of the wave fields and analyze the different characteristics of Rayleigh wave dispersion recorded for the rotational and translational components. Then, we compare the results of single-component inversion with the results of multi-component joint inversion. It is found that the rotational component has wider spectral bands and more higher modes than the translational components, especially at high frequencies; the rotational component has better anti-interference performance in the noisy data test, and it can improve the inversion accuracy of the shallow shear-wave velocity. The field examples also show the significant advantages of the joint utility of the translational and rotational components, especially when a low-velocity layer exists. Rotational observation shall be beneficial for shallow surface-wave exploration. [ABSTRACT FROM AUTHOR]

Nine samples of bed materials along the Jinghong reservoir on the Lancang River were obtained using a gravity sampler. The grain size characteristics of the samples were analyzed by the laser diffraction particle size analyzer. The results show that the median grain size of bed materials is in the range of 6.7 to 18.9 µm. From the upstream to the front of the dam, the overall grain size of the bed materials tends to decrease significantly; the sorting is poor but becomes better along the way; the skewness gradually changes from negative to near symmetrical; the kurtosis is platykurtic and mesokurtic with little change along the way. Based on the measured data, an exponential model is constructed to describe the distribution of representative grain size of bed materials along the way. Furthermore, the concept of representative particle Reynolds number is proposed. The relationship between representative particle Reynolds number and flow parameters with boundary conditions is established, and the coefficient and exponents in the equation are determined based on the measured data of the Jinghong reservoir. This study provides valuable first-hand information for reservoir sediment research and new ideas for sediment sorting and deposition studies. [ABSTRACT FROM AUTHOR]

Angiotensin-I-converting enzyme (ACE) inhibitory peptides derived from marine organism have shown a blood pressure lowering effect with no side effects. A new affinity medium of Fe3O4@ZIF-90 immobilized ACE (Fe3O4@ZIF-90-ACE) was prepared and used in the purification of ACE inhibitory peptides from Wakame (Undaria pinnatifida) protein hydrolysate (<5 kDa). The Fe3O4@ZIF-90 nanoparticles were prepared by a one-pot synthesis and crude ACE extract from pig lung was immobilized onto it, which exhibited excellent stability and reusability. A novel ACE inhibitory peptide, KNFL (inhibitory concentration 50, IC50 = 225.87 μM) was identified by affinity purification using Fe3O4@ZIF-90-ACE combined with reverse phase-high performance liquid chromatography (RP-HPLC) and MALDI-TOF mass spectrometry. Lineweaver–Burk analysis confirmed the non-competitive inhibition pattern of KNFL, and molecular docking showed that it bound at a non-active site of ACE via hydrogen bonds. This demonstrates that affinity purification using Fe3O4@ZIF-90-ACE is a highly efficient method for separating ACE inhibitory peptides from complex protein mixtures and the purified peptide KNFL could be developed as a functional food ingredients against hypertension. [ABSTRACT FROM AUTHOR]

With the penetration of renewable generation, electric vehicles and other random factors in power systems, the stochastic disturbances are increasing significantly, which are necessary to be handled for guarantying the security of systems. A novel stochastic optimal control strategy is proposed in this paper to reduce the impact of such stochastic continuous disturbances on power systems. The proposed method is effective in solving the problems caused by the stochastic continuous disturbances and has two significant advantages. First, a simplified and effective solution is proposed to analyze the system influenced by the stochastic disturbances. Second, a novel optimal control strategy is proposed in this paper to effectively reduce the impact of stochastic continuous disturbances. To be specific, a novel excitation controlled power systems model with stochastic disturbances is built in the quasi-generalized Hamiltonian form, which is further simplified into a lower-dimension model through the stochastic averaging method. Based on this Itô equation, a novel optimal control strategy to achieve the intra-region probability maximization is established for power systems by using the dynamic programming method. Finally, the intra-region probability increases in controlled systems, which confirms the effectiveness of the proposed control strategy. The proposed control method has advantages on controlling the fluctuation of system state variables within a desired region under the influence of stochastic disturbances, which means improving the security of stochastic systems. With more stochasticity in the future, the proposed control method based on the stochastic theory will play a novel way to relieve the impact of stochastic disturbances. [ABSTRACT FROM AUTHOR]

Angiotensin-I-converting enzyme (ACE) inhibitory peptides derived from natural products have shown a blood pressure lowering effect with no side effects. In this study, two novel ACE inhibitory peptides (His-Leu-His-Thr, HLHT and Gly-Trp-Ala, GWA) were purified from pearl oyster ( Pinctada fucata martensii ) meat protein hydrolysate with alkaline protease by ultrafiltration, polyethylene glycol methyl ether modified immobilized metal ion affinity medium, and reverse-phase high performance liquid chromatography. Both peptides exhibited high ACE inhibitory activity with IC 50 values of 458.06 ± 3.24 μM and 109.25 ± 1.45 μM, respectively. Based on the results of a Lineweaver-Burk plot, HLHT and GWA were found to be non-competitive inhibitor and competitive inhibitor respectively, which were confirmed by molecular docking. Furthermore, the pearl oyster meat protein hydrolysate exhibited an effective antihypertensive effect on SD rats. These results conclude that pearl oyster meat protein is a potential resource of ACE inhibitory peptides and the purified peptides, HLHT and GWA, can be exploited as functional food ingredients against hypertension.

Lizard fish protein hydrolysates (LFPH) were prepared from Lizard fish ( Saurida elongata ) proteins possessing powerful angiotensin I converting enzyme (ACE) inhibitory activity and the fraction (LFPH-I) with high ACE inhibitory activity was obtained through ultrafiltration. The active Fraction (F2) was isolated from LFPH-I using immobilized metal affinity chromatography (IMAC - Ni 2+ ). Analysis of amino acid levels revealed that F2 eluted from IMAC was enriched in Met, His, Tyr, Pro, Ile, and Leu compared to the crude peptide LFPH-I. F2 with the high ACE inhibitory activity (IC 50 of 0.116 mg·mL -1 ) was further separated by a reverse-phase column to yield a novel ACE inhibitory peptide with IC 50 value of 52 μM. The ACE inhibitory peptide was identified as Arg-Tyr-Arg-Pro, RYRP. The present study demonstrated that IMAC may be a useful tool for the separation of ACE inhibitory peptides from protein hydrolysate.

Recent evidence suggests that chloride channels are critical for cell proliferation, migration, and differentiation. We examined the effects of transforming growth factor (TGF)-β1 on chloride channel expression and associations with human conjunctival fibroblast (HConF) biology. To investigate the potential role of chloride channel (CLC)-2 in migration, transition to myofibroblasts and extracellular matrix (ECM) synthesis of HconF, a small interfering RNA (siRNA) approach was applied. TGF-β1-induced migration and transition of fibroblasts to myofibroblasts characterized by α-smooth muscle actin (α-SMA) expression, supported by increased endogenous expression of CLC-2 protein and mRNA transcripts. ECM (collagen I and fibronectin) synthesis in HConF was enhanced by TGF-β1. CLC-2 siRNA treatment reduced TGF-β1-induced cell migration, transition of fibroblasts to myofibroblasts, and ECM synthesis of HConF. CLC-2 siRNA treatment in the presence of TGF-β1 inhibited phosphorylation of PI3K and Akt in HConF. These findings demonstrate that CLC-2 chloride channels are important for TGF-β1-induced migration, differentiation, and ECM synthesis via PI3K/Akt signaling in HConF.