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Phosphorus (P) nonpoint source pollution from soil to water is increasing dramatically, leading to the eutrophication of water bodies. Using amendment materials for P retention in soil is a promising strategy for environmental restoration and nonpoint source pollution management. This strategy has attracted significant attention because of its highly effective P retention. This study reviews management strategies of P nonpoint pollution from soil to water, including the basic P forms and accumulation situation in soil and P loss from soil to water. Recent advances in the use of amendment materials, such as inorganic, organic, and composite amendment materials, to mitigate P pollution from soil to water have also been summarized. Environmental risks of reloss of P retention in soil with different soil properties and water conditions have also been investigated. This review improves the understanding of P nonpoint source pollution from soil to water, providing an innovative perspective for the large-scale application of amendment materials to control water eutrophication. [ABSTRACT FROM AUTHOR]

According to the agronomic requirements of garlic sowing, the garlic morphology is studied and a garlic seed metering mechanism with excellent seeding performance is designed. Based on this design, a new garlic seeding machine with an adjustable-size seeding device is developed to realize efficient single-seed metering and seeding of different varieties of garlic. Further, the design scheme of the garlic seeder prototype is established, with the key components of the garlic seeding being designed on the basis of the garlic seeding mechanism. To achieve garlic single-seed metering for different varieties of garlic, the optimal adjustment size of the garlic seed metering device is determined through discrete element simulation analysis. A field experiment confirms the effectiveness of applying the proposed garlic planter to field sowing in terms of the metrics of missing seed and multiple seed rates. The results of the discrete element simulation test reveal that an adjustment size of 40 mm yields the best single-seed metering performance. At an operating speed of 15–35 rpm, the metering device can achieve more than an 80% qualification rate of single-seed metering, with a unit speed of 0.628–1.465 m/s. Thus, the developed garlic seeding device meets the requirements of precision sowing in China and can effectively realize the mechanized planting of garlic. [ABSTRACT FROM AUTHOR]

The therapeutic application of antimicrobial peptides (AMPs), a potential type of peptide-based biomaterial, is impeded by their poor antimicrobial activity and potential cytotoxicity as a lack of understanding of their structure–activity relationships. In order to comprehensively enhance the antibacterial and clinical application potency of AMPs, a rational approach was applied to design amphiphilic peptides, including head-to-tail cyclic, linear and D-proline antimicrobial peptides using the template (IR)nP(IR)nP (n = 1, 2 and 3). Results showed that these amphiphilic peptides demonstrated antimicrobial activity in a size-dependent manner and that cyclic peptide OIR3, which contained three repeating units (IR)3, had greater antimicrobial potency and cell selectivity than liner peptide IR3, DIR3 with D-Pro and gramicidin S (GS). Surface plasmon resonance and endotoxin neutralization assays indicated that OIR3 had significant endotoxin neutralization capabilities, which suggested that the effects of OIR3 were mediated by binding to lipopolysaccharides (LPS). Using fluorescence spectrometry and electron microscopy, we found that OIR3 strongly promoted membrane disruption and thereby induced cell lysis. In addition, an LPS-induced inflammation assay showed that OIR3 inhibited the pro-inflammatory factor TNF-α in RAW264.7 cells. OIR3 was able to reduce oxazolone-induced skin inflammation in allergic dermatitis mouse model via the inhibition of TNF-α, IL-1β and IL-6 mRNA expression. Collectively, the engineered head-to-tail cyclic peptide OIR3 was considerable potential candidate for use as a clinical therapeutic for the treatment of bacterial infections and skin inflammation. [ABSTRACT FROM AUTHOR]

Antibacterial peptides (APMs) are a new type of antibacterial substance. The relationship between their structure and function remains indistinct; in particular, there is a lack of a definitive and fixed template for designing new antimicrobial peptides. Previous studies have shown that porcine Protegrin-1 (PG-1) exhibits considerable antimicrobial activity and cytotoxicity. In this study, to reduce cytotoxicity and increase cell selectivity, we designed histidine-rich peptides based on the sequence template RR(XY)2XDPGX(YX)2RR-NH2, where X represents I, W, V, and F. The results showed that the peptides form more β-hairpin structures in a lipid-rich environment that mimics cell membranes. Among them, the antimicrobial peptide HV2 showed strong antibacterial activity against Gram-negative strains and almost no toxicity to normal cells. The results of our analysis of its antibacterial mechanism showed that peptide HV2 acts on the bacterial cell membrane to increase its permeability, resulting in cell membrane disruption and death. Furthermore, peptide HV2 inhibited bacterial movement in a concentration-dependent manner and had a more robust anti-inflammatory effect by inhibiting the production of TNF-α. In summary, peptide HV2 exhibits high bactericidal activity and cell selectivity, making it a promising candidate for future use as an antibiotic. [ABSTRACT FROM AUTHOR]