Search

Venetoclax and obinutuzumab are becoming frontline therapies for chronic lymphocytic leukemia (CLL) patients. Unfortunately, drug resistance still occurs, and the combination could be immunosuppressive. Lysosomes have previously been identified as a target for obinutuzumab cytotoxicity in CLL cells, but the mechanism remains unclear. In addition, studies have shown that lysosomotropic agents can cause synergistic cell death in vitro when combined with the BTK inhibitor, ibrutinib, in primary CLL cells. This indicates that targeting lysosomes could be a treatment strategy for CLL. In this study, we have shown that obinutuzumab induces lysosome membrane permeabilization (LMP) and cathepsin D release in CLL cells. Inhibition of cathepsins reduced obinutuzumab-induced cell death in CLL cells. We further determined that the lysosomotropic agent siramesine in combination with venetoclax increased cell death in primary CLL cells through an increase in reactive oxygen species (ROS) and cathepsin release. Siramesine treatment also induced synergistic cytotoxicity when combined with venetoclax. Microenvironmental factors IL4 and CD40L or incubation with HS-5 stromal cells failed to significantly protect CLL cells from siramesine- and venetoclax-induced apoptosis. We also found that siramesine treatment inhibited autophagy through reduced autolysosomes. Finally, the autophagy inhibitor chloroquine failed to further increase siramesine-induced cell death. Taken together, lysosome-targeting drugs could be an effective strategy in combination with venetoclax to overcome drug resistance in CLL. [ABSTRACT FROM AUTHOR]

The interaction between fig wasps and their host fig trees (Ficus spp.) is a striking example of an obligate pollination mutualism. Male and female fig wasps are confined within their natal patch instead of panmictic; under this circumstance, mating only occurs between individuals of the same patch. This is known as a local mate competition (LMC). It pays foundresses to invest mainly in daughters and to only produce enough sons to ensure that all female offspring can be fertilized, but in nature, pollinating fig wasps may face many problems with host quality, such as limitation of oviposition sites and the nutrition deficiency of the host fig. The sex ratio of wasps can determine the stability of fig–fig wasp mutualistic system and, thus, the stability of other species associated with it. In this study, we controlled the quality of host figs in three ways. The results showed that the host fig age can influence the sex ratio of pollinator offspring, while the foundress numbers and the presence of pollen have no significant effect on it. A compelling explanation for this result is that the sex-dependent mortality occurs. This is a novel finding of how host quality influences the interaction of fig and fig wasps, which can also help us understand the evolution and stability mechanism of this symbiotic system. [ABSTRACT FROM AUTHOR]

Cells with various structures and proteins naturally come together to cooperate in vivo. This study used cell spheroids cultured in agarose micro-wells as a 3D model to study the movement of cells or spheroids toward other spheroids. The formation dynamics of tumor spheroids and the interactions of two batches of cells in the agarose micro-wells were studied. The results showed that a concave bottom micro-well (diameter: 2 mm, depth: 2 mm) prepared from 3% agarose could be used to study the interaction of two batches of cells. The initial tumor cell numbers from 5 × 103 cells/well to 6 × 104 cells/well all could form 3D spheroids after 3 days of incubation. Adding the second batch of DU 145 cells to the existing DU 145 spheroid resulted in the formation of satellite cell spheroids around the existing parental tumor spheroid. Complete fusion of two generation cell spheroids was observed when the parental spheroids were formed from 1 × 104 and 2 × 104 cells, and the second batch of cells was 5 × 103 per well. A higher amount of the second batch of cells (1 × 104 cell/well) led to the formation of independent satellite spheroids after 48 h of co-culture, suggesting the behavior of the second batch of cells towards existing parental spheroids depended on various factors, such as the volume of the parental spheroids and the number of the second batch cells. The interactions between the tumor spheroids and Human Umbilical Vein Endothelial Cells (HUVECs) were modeled on concave agarose micro-wells. The HUVECs (3 × 103 cell/well) were observed to gather around the parental tumor spheroids formed from 1 × 104, 2 × 104, and 3 × 104 cells per well rather than aggregate on their own to form HUVEC spheroids. This study highlights the importance of analyzing the biological properties of cells before designing experimental procedures for the sequential fusion of cell spheroids. The study further emphasizes the significant roles that cell density and the volume of the spheroids play in determining the location and movement of cells. [ABSTRACT FROM AUTHOR]

Objective: We aimed to quantitatively study the characteristic of diaphragm and chest wall motion using free-breathing dynamic magnetic resonance imaging (D-MRI) in Chinese people with normal lung function. Methods: 74 male subjects (mean age, 37 ± 11 years old) were prospectively enrolled, and they underwent high-resolution CT(HRCT), pulmonary functional tests (PFTs), and D-MRI in the same day. D-MRI was acquired with a gradient-echo sequence during the quiet and deep breathing. The motion of the diaphragm and chest wall were respectively assessed by measuring thoracic anteroposterior diameter (AP), left–right diameter (LR), cranial–caudal diameter (CC), and thoracic area ratios between end-inspiration and end-expiration. The effect of age, body mass index (BMI), and smoking on respiratory muscle function was also analyzed. Results: The mean ratio of right and left AP was greater than that of LR on three transversal planes during both quiet and deep breathing. The mean ratio at the anterior diaphragm (AND, Quiet: 1.04 ± 0.03; Deep: 1.15 ± 0.09) was weaker than that of the apex (vs. APD, Quiet: 1.08 ± 0.05, p < 0.001; Deep: 1.29 ± 0.12, p < 0.001) and posterior diaphragm (vs. POD, Quiet: 1.09 ± 0.04, p < 0.001; Deep: 1.30 ± 0.12, p < 0.001) both in quiet and deep breathing. Compared with non-smokers, the left AP and thoracic area ratios in smokers were significantly decreased (p < 0.05). However, the ratios of AP, LR, CC, and thoracic area on each plane were similar among groups in different age and BMI. Conclusions: During both quiet and deep breathing, the chest wall motion is prominent in the anteroposterior direction. The motions of diaphragm apex and posterior diaphragm were more prominent than that of the anterior diaphragm. Smoking may affect the respiratory muscle mobility. Dynamic MRI can quantitatively evaluate the motion of respiratory muscles. [ABSTRACT FROM AUTHOR]

The secondary metabolites of the phytopathogenic fungus Corynespora cassiicola CC01 from Hevea brasiliensis were investigated. As a result, two new compounds, 5-acetyl-7-hydroxy-6- methoxybenzofuran-2(3H)-one (1) and (S)-2-(2,3-dihydrofuro [3,2-c]pyridin-2-yl)propan-2-ol (2), together with seven known compounds, 4,6,8-trihydroxy-3,4-dihydronaphthalen-1(2H)-one (3), 3,6,8-trihydroxy-3,4-dihydronaphthalen-1(2H)-one (4), curvulin acid (5), 2-methyl-5-carboxymethyl- 7-hydroxychromone (6), tyrosol (7), p-hydroxybenzoic acid (8) and cerevisterol (9), were isolated from the fermentation extract by comprehensive silica gel, reverse phase silica gel, Sephadex-LH20 column chromatography and high-performance liquid chromatography (HPLC). The structures of these compounds were identified by using high-resolution electrospray mass spectrometry (HRESIMS), nuclear magnetic resonance spectroscopy (NMR), optical rotation, ultraviolet and infrared spectroscopy techniques and a comparison of NMR data with those reported in the literature. Compounds 1 and 2 were new compounds, and compounds 3–9 were discovered from this phytopathogenic fungus for the first time. Compounds 1–9 were tested for phytotoxicity against the fresh tender leaf of Hevea brasiliensis, and the results show that none of them were phytotoxic. Additionally, these compounds were subjected to an antimicrobial assay against three bacteria (E. coli, methicillin-resistant Staphylococcus aureus and Micrococcus luteus), but they showed no activity. [ABSTRACT FROM AUTHOR]

Tea polysaccharide (TPS) is the second most abundant ingredient in tea following tea polyphenols. As a complex polysaccharide, TPS has a complex chemical structure and a variety of bioactivities, such as anti-oxidation, hypoglycemia, hypolipidemic, immune regulation, and anti-tumor. Additionally, it shows excellent development and application prospects in food, cosmetics, and medical and health care products. However, numerous studies have shown that the bioactivity of TPS is closely related to its sources, processing methods, and extraction methods. Therefore, the authors of this paper reviewed the relevant recent research and conducted a comprehensive and systematic review of the extraction methods, physicochemical properties, and bioactivities of TPS to strengthen the understanding and exploration of the bioactivities of TPS. This review provides a reference for preparing and developing functional TPS products. [ABSTRACT FROM AUTHOR]

A tapered pile (TP) is a new type of pile with a good bearing capacity, and scholars have conducted in-depth research on its static bearing characteristics. However, there is relatively little research on its dynamic bearing characteristics. In this paper, the horizontal vibration behavior of a tapered pile in arbitrarily layered soil is studied. Utilizing the Winkler foundation model and Timoshenko beam model to simulate pile-surrounding soil (PSS) and a tapered pile, respectively, the horizontal vibration model of a tapered pile embedded in layered soil was built. The analytical solutions for the horizontal displacement (HD), bending moment (BM), and shear force (SF) of a tapered pile were derived, and then the solutions for the horizontal dynamic impedance (HDI), rocking dynamic impedance (RDI), and horizontal-rocking coupling dynamic impedance (HRDI) of pile head were obtained. Using the present solutions, the effects of soil and pile properties on the horizontal vibration characteristics of a tapered pile were systemically studied. The ability of a tapered pile–soil system to resist horizontal vibration can be improved by strengthening the upper soil, but this ability cannot be further improved by increasing the thickness of the strengthened upper soil if its thickness is greater than the critical influence thickness. [ABSTRACT FROM AUTHOR]

The vertical vibration of a viscoelastic pile immersed in arbitrarily layered soil is investigated by taking the interaction among pile, pile surrounding soil (PSS) and pile end soil (PES) into account. Firstly, considering both the stratification and stress wave effect of soil, a mathematical model of the pile–soil system is established based on the fictitious soil pile (FSP) model. Then, utilizing the impedance function transfer method and Laplace transform technique, the analytical solutions of the vertical dynamic impedance of pile are derived in the frequency domain. The analytical solutions are validated by comparing them with other existing solutions. Finally, a parametric study is put forward to investigate the properties of PES on the vertical dynamic impedance of pile. The results reveal that the properties of PES have a significant effect on the vertical dynamic impedance of pile, but there is a critical influence thickness for this effect. For the cases of the PES thickness exceeding the critical influence thickness, further increase of PES thickness will not affect the dynamic behavior of the pile–soil system. [ABSTRACT FROM AUTHOR]

Morchella esculenta (L.) Pers., referred to as Morel, is a medicinal and edible homologous fungus, which contains many bioactive substances. In Morel, polysaccharides are the most abundant and have various bioactivities. In the present work, two novel polysaccharides, Se-MPS and MPS, were prepared and purified from selenium-enriched (Se-enriched) and common Morel mycelia, respectively, and their structural and immunomodulatory properties were evaluated. The results show that Se-enriched treatment significantly changed the polysaccharides' chemical composition, molecular weight, and sugar chain configuration. In addition, the Se-enriched treatment also improved the polysaccharides' fragmentation and thermal stability. Importantly, Se-enriched Morel polysaccharide (Se-MPS) could significantly enhance phagocytosis of RAW 264.7 macrophage cells and, remarkably, activate their immune response via activating the TLR4-TRAF6-MAPKs-NF-κB cascade signaling pathway, finally exerting an immunomodulatory function. Based on these findings, selenium-enriched Morel polysaccharide appears to have more potential for development and utilization in functional foods or medicines than ordinary Morel polysaccharide. [ABSTRACT FROM AUTHOR]

Objective: Current studies focus on the prevalence rate of lung cancer in idiopathic pulmonary fibrosis and connective tissue disease-associated interstitial lung disease (CTD-LID). Our aim was to investigate the prevalence of malignancies in patients with various subtypes of ILD. Methods: A total of 5350 patients diagnosed with ILD between January 2015 and December 2021 were retrospectively included. The prevalence of different malignancies and different ILDs was assessed using complete follow-up data. Results: A total of 248 patients (139 males; 65—IQR, 57 to 72—years) out of 5350 patients with ILD were confirmed with malignancies. A total of 69% of patients with ILD and malignances were older than 60 years old. The prevalence of malignancies in ILD patients was 4.6%, and lung cancer had the most common incidence of 1.9%, followed by malignancies in the digestive system of 0.9%. Among the different ILD subtypes, the prevalence of malignancies such as organizing pneumonia (OP), idiopathic pulmonary fibrosis (IPF), anti-neutrophil cytoplasmic antibodies-associated vasculitis-related ILD(AAV-ILD), nonspecific interstitial pneumonia (NSIP), CTD-ILD, hypersensitivity pneumonitis (HP), sarcoidosis, and other types of ILD was 6.8%, 5.0%, 4.7%, 4.3%, 2.5%, 2.2%, 1.2%, and 6.9%, respectively. The incidence of lung cancer as the most common tumor in IPF was 3.9%, with adenocarcinoma predominating (1.7%). The highest rate of malignancy occurring in RA of CTD-ILD was 2.4%. Conclusion: Older patients with ILD (≥60 years) including OP, IPF, AAV-ILD, NSIP, CTD-ILD, and HP, were associated with a higher incidence of malignancy, especially males aged from 60 to 69 years. These epidemiological results indicate that it is essential for physicians to pay more attention to the screening of and management strategies for different malignancies, according to the specific ILD subtypes. [ABSTRACT FROM AUTHOR]

The dynamic characteristics of pipe piles are of considerable importance for the dynamic foundation design of offshore wind turbines. In this study, we develop an analytical model for the lateral vibration of offshore pipe piles with consideration of the inertia effect and axial loading from the superstructure. A coupled dynamic saturated soil–pile interaction model is established based on Biot's poroelastic theory and Euler–Bernoulli theory. The potential function, operator decomposition method, variable separation method and matrix transfer method are introduced herein to obtain the lateral force of the inner and outer soil acting on the pile shaft. Then, the analytical solution of the pile dynamic impedance in the frequency domain is derived by employing the soil–pile continuous deformation conditions and the boundary conditions of the pile. The rationality and accuracy of the presented solution have were by comparing its results with those predicted by existing solutions. The influence of superstructure, pile geometry and soil plug height on the lateral dynamic impedance and natural frequency of pipe piles was thoroughly investigated based on the theoretical model. The main findings can be summarized as: (1) The dynamic stiffness of piles will be remarkably underestimated if the inertia effect of the superstructure is not accounted for. (2) The vertical load of the superstructure is main factor affecting the natural frequency, whereas the inertia effect of the superstructure will enlarge the resonance amplitude. (3) The overall lateral dynamic impedance and first-order natural frequency of the pile increase significantly with the soil plug height. [ABSTRACT FROM AUTHOR]

The research on the deformation mechanism of monopile foundation supporting offshore wind turbines is significant to optimize the design of a monopile foundation under wave and current load. In this paper, a three-dimensional wave-pile-soil coupling finite element model is proposed to investigate the deformation mechanism of monopile undercurrent and fifth-order Stokes wave. Different from the conventional assumption that there is no slip at the pile-soil interface, Frictional contact is set to simulate the relative movement between monopile and soil. Numerical results indicate that under extreme environmental conditions, the monopile foundation sways within a certain range and the maximum displacement in the loading direction is 1.3 times the displacement in the reverse direction. A further investigation has been made for a large-diameter pipe pile with various design parameters. The finite element analyses reveal that the most efficient way to reduce the deflection of the pile head is by increasing the embedment depth of the monopile. When the embedment depth is limited, increasing the pile diameter is a more effective way to strengthen the foundation than increasing the wall thickness. [ABSTRACT FROM AUTHOR]

The Advanced Spaceborne Thermal-Emission and Reflection Radiometer Global Digital Elevation Model (ASTER GDEM) is important to a wide range of geographical and environmental studies. Its accuracy, to some extent associated with land-use types reflecting topography, vegetation coverage, and human activities, impacts the results and conclusions of these studies. In order to improve the accuracy of ASTER GDEM prior to its application, we investigated ASTER GDEM errors based on individual land-use types and proposed two linear regression calibration methods, one considering only land use-specific errors and the other considering the impact of both land-use and topography. Our calibration methods were tested on the coastal prefectural city of Lianyungang in eastern China. Results indicate that (1) ASTER GDEM is highly accurate for rice, wheat, grass and mining lands but less accurate for scenic, garden, wood and bare lands; (2) despite improvements in ASTER GDEM2 accuracy, multiple linear regression calibration requires more data (topography) and a relatively complex calibration process; (3) simple linear regression calibration proves a practicable and simplified means to systematically investigate and improve the impact of land-use on ASTER GDEM accuracy. Our method is applicable to areas with detailed land-use data based on highly accurate field-based point-elevation measurements. [ABSTRACT FROM AUTHOR]

Conidia fusion (CF) is a commonly observed structure in fungi. However, it has not been systematically studied. This study examined 2457 strains of nematode-trapping fungi (NTF) to explore the species specificity, physiological period, and physiological significance of CF. The results demonstrated that only six species of Arthrobotrys can form CF among the sixty-five tested NTF species. The studies on the model species Arthrobotrys oligospora (DL228) showed that CF occurred in both shed and unshed plus mature and immature conidia. Additionally, the conidia fusion rate (CFR) increased significantly with the decrease of nutrient concentration in habitats. The studies on the conidia fusion body (CFB) produced by A. oligospora (DL228) revealed that the more conidia contained in the CFB, the faster and denser the mycelia of the CFB germinated in weak nutrient medium and soil plates. On the one hand, rapid mycelial extension is beneficial for the CFB to quickly find new nutrient sources in habitats with uneven nutrient distribution. On the other hand, dense mycelium increases the contact area with the environment, improving the nutrient absorption efficiency, which is conducive to improving the survival rate of conidia in the weak nutrient environment. In addition, all species that form CF produce smaller conidia. Based on this observation, CF may be a strategy to balance the defects (nutrient deficiency) caused by conidia miniaturization. [ABSTRACT FROM AUTHOR]

Urban green space takes a dominant role in alleviating the urban heat island (UHI) effect. Most investigations into the effects of cooling factors from urban green spaces on the UHI have evaluated the correlation between each factor and land surface temperature (LST) separately, and the contribution weights of various typical cooling factors in mitigating the thermal environment have rarely been analyzed. For this research, three periods of Landsat 8 data captured between 2014 and 2018 of Xuzhou during the summer and autumn seasons were selected along with corresponding meteorological and flux measurements. The mono-window method was employed to retrieve LST. Based on the characteristics of the vegetation and spatial features of the green space, eight factors related to green space were selected and computed, consisting of three indices that measure vegetation and five metrics that evaluate landscape patterns: vegetation density (VD), evapotranspiration (ET), green space shading degree (GSSD), patch area ratio (PLAND), largest patch index (LPI), patch natural connectivity (COHESION), patch aggregation (AI), and patch mean shape index distribution (SHPAE_MN). Linear regression and bivariate spatial autocorrelation analyses between each green space factor and LST showed that there were significant negative linear and spatial correlations between all factors and LST, which proved that the eight factors were all cooling factors. In addition, LST was strongly correlated with all factors (|r| > 0.5) except for SHPAE_MN, which was moderately correlated (0.3 < |r| < 0.5). Based on this, two principal components were extracted by applying principal component analysis with all standardized green space factors as the original variables. To determine the contribution weight of each green space factor in mitigating the urban heat island (UHI) effect, we multiplied the influence coefficient matrix of the initial variables with the standardized multiple linear regression coefficients between the two principal component variables and LST. The final results indicated that the vegetation indices of green space contribute more to the alleviation of the UHI than its landscape pattern metrics, and the contribution weights are ranked as VD ≥ ET > GSSD > PLAND ≈ LPI > COHESION > AI > SHAPE_MN. Our study suggests that increasing vegetation density is preferred in urban planning to mitigate urban thermal environment, and increasing broadleaf forests with high evapotranspiration and shade levels in urban greening is also an effective way to reduce ambient temperature. For urban green space planning, a priority is to multiply the regional green space proportion or the area of largest patches. Second, improving the connectivity or aggregation among patches of green space can enhance their ability to cool the surrounding environment. Altering the green space spatial shape is likely the least significant factor to consider. [ABSTRACT FROM AUTHOR]

Edible fungi, commonly known as mushrooms, are precious medicinal and edible homologous gifts from nature to us. Because of their distinctive flavor and exceptional nutritional and medicinal value, they have been a frequent visitor to people's dining tables and have become a hot star in the healthcare, pharmaceutical, and cosmetics industries. Edible fungal polysaccharides (EFPs) are an essential nutrient for edible fungi to exert bioactivity. They have attracted much attention because of their antioxidant, immunomodulatory, antitumor, hypoglycemic, and hypolipidemic bioactivities. As a result, EFPs have demonstrated outstanding potential over the past few decades in various disciplines, including molecular biology, immunology, biotechnology, and pharmaceutical chemistry. However, the complexity of EFPs and the significant impact of mushroom variety and extraction techniques on their bioactivities prevents a complete investigation of their biological features. Therefore, the authors of this paper thoroughly reviewed the comparison of different extraction methods of EFPs and their advantages and disadvantages. In addition, the molecular weight, monosaccharide composition, and glycosidic bond type and backbone structure of EFPs are described in detail. Moreover, the in vitro and in vivo bioactivities of EFPs extracted by different methods and their potential regulatory mechanisms are summarized. These provide a valuable reference for improving the extraction process of EFPs and their production and development in the pharmaceutical field. [ABSTRACT FROM AUTHOR]

The appearance characteristics of ceramic color are an important factor in determining the user's aesthetic perception of the product. Given the problem that ceramic color varies and the user's visual sensory evaluation of color is highly subjective and uncertain, a method of quantifying ceramic color characteristics based on the Back Propagation (BP) neural network algorithm is proposed. The semantic difference method and statistical method were used to obtain quantified data from ceramic color perceptual semantic features and were combined with a neural network to study the association between ceramic color features and user perceptual-cognitive evaluation. A BP neural network was used to build a ceramic color perceptual semantic mapping model, using color semantic quantified values as the input layer, color L, A, and B component values as the output layer, and model training to predict the sample. The output color L, A, and B components are used as the input layer and the color scheme was designed. The above method can effectively solve the mapping problem between the appearance characteristics of ceramic color and perceptual semantics and provide a decision basis for ceramic product color design. The case application of color design of daily-use ceramic products was conducted to verify the effectiveness and feasibility of the quantitative research method of ceramic color imagery. [ABSTRACT FROM AUTHOR]

Global Navigation Satellite System (GNSS) tomography is a popular method for measuring and modelling water vapor in the troposphere. Presently, most studies use a cuboid-shaped tomographic region in their modelling, which represents the modelling region for all measurement epochs. This region is defined by the distribution of the GNSS signals skywards from a network of ground based GNSS stations for all epochs of measurements. However, in reality at each epoch the shape of the GNSS tomographic region is more likely to be an inverted cone. Unfortunately, this fixed conic tomographic region does not properly reflect the fact that the GNSS signal changes quickly over time. Therefore a dynamic or adaptive tomographic region is better suited. In this study, a new approach that adjusts the GNSS tomographic model to adapt the size of the GNSS network is proposed, which referred to as The High Flexibility GNSS Tomography (HFGT). Test data from different numbers of the GNSS stations are used and the results from HFGT are compared against that of radiosonde data (RS) to assess the accuracy of the HFGT approach. The results showed that the new approach is feasible for different numbers of the GNSS stations when a sufficient and uniformed distribution of GNSS signals is used. This is a novel approach for GNSS tomography. [ABSTRACT FROM AUTHOR]

As an important energy absorption process in the Earth's surface energy balance, evapotranspiration (ET) from vegetation and bare soil plays an important role in regulating the environmental temperatures. However, little research has been done to explore the cooling effect of ET on the urban heat island (UHI) due to the lack of appropriate remote-sensing-based estimation models for complex urban surface. Here, we apply the modified remote sensing Penman–Monteith (RS-PM) model (also known as the urban RS-PM model), which has provided a new regional ET estimation method with the better accuracy for the urban complex underlying surface. Focusing on the city of Xuzhou in China, ET and land surface temperature (LST) were inversed by using 10 Landsat 8 images during 2014–2018. The impact of ET on LST was then analyzed and quantified through statistical and spatial analyses. The results indicate that: (1) The alleviating effect of ET on the UHI was stronger during the warmest months of the year (May–October) but not during the colder months (November–March); (2) ET had the most significant alleviating effect on the UHI effect in those regions with the highest ET intensities; and (3) in regions with high ET intensities and their surrounding areas (within a radius of 150 m), variation in ET was a key factor for UHI regulation; a 10 W·m−2 increase in ET equated to 0.56 K decrease in LST. These findings provide a new perspective for the improvement of urban thermal comfort, which can be applied to urban management, planning, and natural design. [ABSTRACT FROM AUTHOR]

Herein, novel hyperbranched polyamidoamine guanidinium salts (GS-h-PAMAM) and two cationic acrylamide copolymers P(AM-DAC-ABSM) and P(AM-DAC-AMTU) were successfully prepared. Then, self-assembly supramolecular systems were synthesized by directly mixing GS-h-PAMAM with copolymers in aqueous solution, and the mechanism of the self-assembly process was speculated. FT-IR, NMR, and SEM were used for structural confirmation. Furthermore, the excellent solution properties revealed that the supramolecular systems had potential application in clay hydration inhibitors. More importantly, utilizing functionalized hyperbranched polyamidoamine in the synthesis self-assembly supramolecular systems was an effective strategy for expanding their application fields and developing new functional materials, providing a powerful reference for the next study. [ABSTRACT FROM AUTHOR]

Development of simple, convenient, and sensitive assay methods for pyrophosphatase (PPase) activity is of importance, for disease diagnosis and drug discovery. Herein, a simple, rapid, label-free, and sensitive fluorescence sensor for PPase activity assay is developed, using Cu2+ doping-induced quantum dot (QD) photoluminescence as a signal reporter. The Cu2+ doping of ZnSe QD can induce a dopant-dependent emission response, which will be inhibited after the premixing of Cu2+ with pyrophosphate (PPi), to form a Cu2+-PPi complex. Then, the hydrolysis of PPi into phosphate (Pi), specifically catalyzed by PPase, liberates the free Cu2+ to regain the QD doping for the fluorescence response, which is highly dependent on the PPase activity. The PPase can be sensitively and selectively assayed, with a detection limit of 0.1 mU/mL. The developed sensing strategy can be also employed for the PPase inhibitor screening. Thus, the current QD doping-based sensing strategy offers an efficient and promising avenue for Cu2+, PPi, or PPase-related target analysis, and might hold great potential for the further applications in the clinical disease diagnosis. [ABSTRACT FROM AUTHOR]

Allelochemicals are the media of allelopathy and form the chemical bases of plant-environment interactions. To determine true allelochemicals and their autotoxic effects, seven compounds were isolated and identified from in-situ sampled rhizosphere soil of cultivated Saussurea lappa. Of these; costunolide (2), dehydrocostus lactone (3) and scopoletin (4) showed significant inhibition on seedling growth in a concentration-dependent manner. Detection and observation demonstrated that the antioxidase system was found to be affected by these chemicals, resulting in the accumulation of ROS and membrane damage. To investigate their release ways, the compounds were traced back and volumes quantified in rhizosphere soil and plant tissues. This work made clear the chemical bases and their physiological effects on the plants. These chemicals were found to be the secondary metabolites of the plants and included in the rhizosphere soil. The findings identified a potential pathway of plant-plant interactions, which provided theoretical basis to overcoming replanting problems. This research was also useful for exploring ecological effects of allelochemicals in green agriculture. [ABSTRACT FROM AUTHOR]

The amount and growth rate of carbon emissions have been accelerated on a global scale since the industrial revolution (1800), especially in recent decades. This has resulted in a significant influence on the natural environment and human societies. Therefore, carbon emission reduction receives continuously increasing public attention and has long been under debate. In this study, we made use of the land-use specific carbon emission coefficients from previous studies and estimated the land-use carbon emissions and carbon intensities of the Yangtze River Delta Urban Agglomeration (YRDUA)—which consists of 26 eastern Chinese cities—from Landsat image data and socio-economic statistics in 1995, 2005, and 2015. In addition, spatial autocorrelation models including both global and local Moran's I were used to analyze the spatial autocorrelation of carbon emissions and carbon intensities. It was found that (1) the YRDUA witnessed a rapidly increasing trend for net carbon emissions and a decreasing trend for carbon intensity over the two decades; (2) the spatial differences in carbon intensity had gradually narrowed, but were large in carbon emissions and had gradually increased; and (3) the carbon emissions in 2005 and 2015 had significant spatial autocorrelations. We concluded that (1) from 1995 to 2015 in the YRDUA, carbon emissions were large for the cities along the Yangtze River and carbon intensities were high for Anhui province's resource-based cities, while both carbon emissions and carbon intensities were small for Zhejiang province's cities; (2) over two decades, the increase in carbon emissions from urban land was approximately twice the increase in urban land area. Our study can provide useful insights into the assignment of carbon reduction tasks within the YRDUA. [ABSTRACT FROM AUTHOR]

The present study aims to optimize the ethanol-reflux extraction conditions for extracting saponins from steamed Panax notoginseng (SPN). Four variables including the extraction time (0.5–2.5 h), ethanol concentration (50–90%), water to solid ratio (W/S, 8–16), and times of extraction (1–5) were investigated by using the Box-Behnken design response surface methodology (BBD-RSM). For each response, a second-order polynomial model with high R2 values (>0.9690) was developed using multiple linear regression analysis and the optimum conditions to maximize the yield (31.96%), content (70.49 mg/g), and antioxidant activity (EC50 value of 0.0421 mg/mL) for saponins extracted from SPN were obtained with a extraction time of 1.51 h, ethanol concentration of 60%, extraction done 3 times, and a W/S of 10. The experimental values were in good consistency with the predicted ones. In addition, the extracted SPN saponins could significantly increase the levels of blood routine parameters compared with the model group (p < 0.01) and there was no significant difference in the hematopoiesis effect between the SPN group and the SPN saponins group, of which the dose was 15 times lower than the former one. It is suggested that the SPN saponins extracted by the optimized method had similar functions of “blood tonifying” at a much lower dose. [ABSTRACT FROM AUTHOR]