415 results on '"Yang Kun"'
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2. Preparation and Performance Study of SiC-Reinforced Fe-Based Wear-Resistant Composite Grinding Media.
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Yang, Kun and Zhou, Yongmin
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ENGINEERING laboratories , *WEAR resistance , *MECHANICAL wear , *MANUFACTURING processes , *PERFORMANCE theory , *POWDER metallurgy , *HOT pressing - Abstract
During industrial and laboratory processes involving material grinding, the grinding media endure prolonged high-collision and friction environments, resulting in substantial wear. Consequently, this study adopts the hot-pressing sintering technique in powder metallurgy to prepare SiC-reinforced Fe-based wear-resistant composite grinding media, aiming to increase wear performance. For this purpose, Fe with 10 wt% SiC powders were milled for the fabrication of the composite. Then, sintering was performed by hot press at 1100 °C in a furnace. Scanning electron microscopy (SEM) and X-ray diffraction were employed to investigate the microstructures and phase of SiC-reinforced Fe-based matrix composite. Subsequently, comparative performance evaluations of the newly developed grinding media and traditional chromium-based media were conducted in terms of wear rate and grinding efficiency. The wear resistance tests revealed that the SiC-reinforced composite media displayed significantly superior wear resistance across various abrasives compared to the chromium-containing alternatives. Specifically, the composite media achieved a wear rate reduction of 2.9 times against standard sand over 1 h, and 2.3 and 2.4 times against sandstone and iron slag, respectively. Moreover, extended grinding for 3 hours further enhanced these reductions to 3.1, 2.4, and 2.7 times, respectively. Additionally, efficiency assessments indicated that at a 1:1 material ratio, the composite media outperformed the chromium-containing media in grinding efficiency by 7.5%, 12.5%, and 10.3% for standard sand, sandstone, and iron slag, respectively. Further increasing the material ratio to 3:1 resulted in efficiency improvements of 7.4%, 17.5%, and 11.3%, correspondingly. [ABSTRACT FROM AUTHOR]
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- 2024
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3. Influence of Pump Light on LP 01 and LP 11 Modes in Few-Mode Fiber Brillouin Optical Time Domain Reflectometry.
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Hao, Yunqi, Han, Yiliang, Liao, Weitong, Miao, Miao, and Yang, Kun
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OPTICAL time-domain reflectometry ,REFLECTOMETRY ,BRILLOUIN scattering ,MODE-locked lasers ,OPTICAL fibers ,FIBER lasers ,NATURAL fibers - Abstract
The quality of pump pulse in few-mode-fiber Brillouin optical time domain reflectometry (FMF-BOTDR) is vital for the spontaneous Brillouin scattering of modes LP
01 and LP11 because it is the comprehensive effect of the main laser linewidth and pulse width, which is firstly discussed as we know. Numerical and experimental analysis are made for the amplitude and linewidth distribution, corresponding to the signal–noise ratio (SNR) and frequency resolution in BOTDR, respectively. Simulation shows the linewidths and peak values of Brillouin scattering have the same tendency for the LP01 mode and LP11 mode when the laser linewidth is less than 1 MHz but decreases slowly until they are the same when the laser linewidth is wider than 1 MHz. With the pulse width widening, the Brillouin linewidths for LP01 and LP11 modes both decrease sharply, almost to the natural linewidth of fiber 41 MHz and 35 MHz. Experimental results show that the amplitude distribution for the LP01 mode is always larger than for the LP11 mode if the main laser has the same linewidth and the frequency fluctuation is at least 2 MHz with the fiber laser and LP11 mode. The above results could provide improved sensing resolution for FMF-BOTDR sensing system. [ABSTRACT FROM AUTHOR]- Published
- 2024
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4. Risk and Energy Based Optimization for Fire Monitoring System in Utility Tunnel Using Cellular Automata.
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Zhang, Ying, Bai, Jitao, Diao, Yu, Chen, Zhonghao, Wang, Chu, Yang, Kun, Gao, Zeng, and Wei, Huajie
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Fire is one of the biggest threats to the safety of utility tunnels, and establishing camera-based monitoring systems is conducive to early fire finding and better understanding of the evolution of tunnel fires. However, conventional monitoring systems are being faced with the challenge of high energy consumption. In this paper, the camera operation in a utility tunnel was optimized considering both fire risk and energy consumption. Three design variables were investigated, namely the camera sight, the number of cameras in simultaneous operation, and the duration of camera operation. Cellular automata were used as a simple but effective method to simulate the spread of fire in a utility tunnel. Results show that as the number of cameras in simultaneous operation increases, the probability of fire capture also increases, but the energy consumption decreases. A shorter duration of camera operation can lead to a higher probability of fire capture, and meanwhile, lower energy consumption. For the duration of camera operation shorter than or equal to the allowable time, the probability of fire capture is significantly higher than that for the duration longer than the allowable time. Increasing the camera sight will significantly increase the probability of fire capture and lower the total energy consumption when a blind monitoring area exists. The total energy consumption of a camera-based monitoring system roughly satisfies hyperbolic correlation with the duration of camera operation, while the probability of fire capture can be predicted based on the number of cameras in simultaneous operation through a power model. The optimal design for the modeled tunnel section is two cameras in simultaneous operation with a tangent monitoring area. The duration of camera operation should be as short as possible, at least shorter than the allowable time. The study is expected to provide a reference for the sustainable design of energy-saving utility tunnels with lower fire risk. [ABSTRACT FROM AUTHOR]
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- 2024
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5. Efficient Biodegradation of the Neonicotinoid Insecticide Flonicamid by Pseudaminobacter salicylatoxidans CGMCC 1.17248: Kinetics, Pathways, and Enzyme Properties.
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Zhao, Yun-Xiu, Yuan, Jing, Song, Ke-Wei, Yin, Chi-Jie, Chen, Li-Wen, Yang, Kun-Yan, Yang, Ju, and Dai, Yi-Jun
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INSECTICIDES ,BIODEGRADATION ,ENZYMES ,AMIDE derivatives ,MOLECULAR docking ,ENZYME kinetics ,NEONICOTINOIDS ,IMIDACLOPRID - Abstract
Nitrile-containing insecticides can be converted into their amide derivatives by Pseudaminobacter salicylatoxidans. N-(4-trifluoromethylnicotinoyl) glycinamide (TFNG-AM) is converted to 4-(trifluoromethyl) nicotinoyl glycine (TFNG) using nitrile hydratase/amidase. However, the amidase that catalyzes this bioconversion has not yet been fully elucidated. In this study, it was discovered that flonicamid (FLO) is degraded by P. salicylatoxidans into the acid metabolite TFNG via the intermediate TFNG-AM. A half-life of 18.7 h was observed for P. salicylatoxidans resting cells, which transformed 82.8% of the available FLO in 48 h. The resulting amide metabolite, TFNG-AM, was almost all converted to TFNG within 19 d. A novel amidase-encoding gene was cloned and overexpressed in Escherichia coli. The enzyme, PmsiA, hydrolyzed TFNG-AM to TFNG. Despite being categorized as a member of the amidase signature enzyme superfamily, PsmiA only shares 20–30% identity with the 14 previously identified members of this family, indicating that PsmiA represents a novel class of enzyme. Homology structural modeling and molecular docking analyses suggested that key residues Glu247 and Met242 may significantly impact the catalytic activity of PsmiA. This study contributes to our understanding of the biodegradation process of nitrile-containing insecticides and the relationship between the structure and function of metabolic enzymes. [ABSTRACT FROM AUTHOR]
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- 2024
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6. Response Surface Methodology for Development of Nutrient Solution Formula for Hydroponic Lettuce Based on the Micro-Elements Fertilizer Requirements at Different Growth Stages.
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Gong, Binbin, Ren, Xiaowei, Hao, Wenyu, Li, Jingrui, Hou, Shenglin, Yang, Kun, Wu, Xiaolei, and Gao, Hongbo
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RESPONSE surfaces (Statistics) ,LETTUCE ,SOIL fertility ,FERTILIZERS ,IRRIGATION water ,SOIL salinity - Abstract
In order to precisely obtain the impact of nutritional elements on lettuce yield and quality, in the present study, a nutrient solution formula suitable for lettuce hydroponic production was development using response surface methodology based on the determination for micro-elements in three growth stages and taking the interaction between elements into account. Then, the formula was optimized and validated, aiming for the goal of improving lettuce yield and quality. The results showed that 200 healthy lettuce leaves contained similar amounts of macro-elements, and there was no significant difference in the unit content of micro-elements among the seedling, rosette, and harvest stages. Quadratic regression models between shoot fresh weight, SPAD value, soluble sugar content, Vc content, and nutrient content were established (R
2 = 0.91, 0.95, 0.98, and 0.81, respectively). The optimal concentrations of P, K, Ca, and Mg obtained by multi-objective optimization of the quadratic regression models for fresh weight, SPAD value, soluble sugar content, and Vc content were 2.71 mmol·L−1 , 6.42 mmol·L−1 , 5.58 mmol·L−1 , and 7.11 mmol·L−1 , respectively. The nutrient solution formula (T1) was found to be the optimal nutrient solution formula for improving lettuce growth and quality. Overall, we developed a specific and targeted nutrient solution formulation for lettuce; this formulation not only meets lettuce's demand for nutrients, but also improves lettuce yield and quality, providing more choices for lettuce production in a region with high salts and high pH in the irrigation water. [ABSTRACT FROM AUTHOR]- Published
- 2024
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7. Improving the Accuracy of Direction of Arrival Estimation with Multiple Signal Inputs Using Deep Learning.
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Lu, Yihan, Guan, Hengchao, Yang, Kun, Peng, Tong, Wen, Chengyuan, and Li, Xin
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DEEP learning ,DIRECTION of arrival estimation ,CONVOLUTIONAL neural networks ,STANDARD deviations ,NOISE control - Abstract
In this paper, an innovative cyclic noise reduction method and an improved CAPON algorithm (also the called minimum variance distortionless response (MVDR) algorithm) are proposed to improve the accuracy and reliability of DOA (direction of arrival) estimation. By processing the eigenvalues obtained from the covariance matrix of the received signal, the signal-to-noise ratio (SNR) can be increased by up to 5 dB by the cyclic noise reduction method, which will improve the DOA estimation accuracy. The improved CAPON algorithm has a convolution neural network (CNN) structure, whose input is the processed covariance matrix of the received signal, and the CAPON spectral value is used as the training label to obtain the estimated spatial spectrum. It retains the advantages of the CAPON algorithm, which can achieve blind source estimation, and simulations show that the proposed algorithm exhibits a better performance than the traditional algorithm in conditions of various SNRs and snapshot numbers. The simulation results show that, based on a certain SNR, the root mean square error (RMSE) of the improved CAPON algorithm can be reduced from 0.86° to 0.8° compared to traditional algorithms, and the angle estimation error can be decreased by up to about 0.3°. With the help of the cyclic noise reduction method, the angle estimation error decreases from 0.04° to 0.02°. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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8. Self-Supervised Joint Learning for pCLE Image Denoising †.
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Yang, Kun, Zhang, Haojie, Qiu, Yufei, Zhai, Tong, and Zhang, Zhiguo
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IMAGE denoising , *LASER endoscopy , *FLUORESCENCE microscopy , *DEEP learning - Abstract
Probe-based confocal laser endoscopy (pCLE) has emerged as a powerful tool for disease diagnosis, yet it faces challenges such as the formation of hexagonal patterns in images due to the inherent characteristics of fiber bundles. Recent advancements in deep learning offer promise in image denoising, but the acquisition of clean-noisy image pairs for training networks across all potential scenarios can be prohibitively costly. Few studies have explored training denoising networks on such pairs. Here, we propose an innovative self-supervised denoising method. Our approach integrates noise prediction networks, image quality assessment networks, and denoising networks in a collaborative, jointly trained manner. Compared to prior self-supervised denoising methods, our approach yields superior results on pCLE images and fluorescence microscopy images. In summary, our novel self-supervised denoising technique enhances image quality in pCLE diagnosis by leveraging the synergy of noise prediction, image quality assessment, and denoising networks, surpassing previous methods on both pCLE and fluorescence microscopy images. [ABSTRACT FROM AUTHOR]
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- 2024
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9. Evolution of the Microstructure and Mechanical Properties of a Biomedical Ti-20Zr-40Ta Alloy during Aging Treatment.
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Wu, Xueqing, Yang, Kun, Cheng, Jun, and Lin, Jianguo
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ALLOYS ,MICROSTRUCTURE ,MARTENSITIC transformations ,DEFORMATIONS (Mechanics) ,RECRYSTALLIZATION (Metallurgy) ,TITANIUM alloys - Abstract
The research focus in the field of medical titanium alloys has recently shifted towards the development of low-modulus and high-strength titanium alloys. In this study, the influence of aging temperature on the microstructure and mechanical properties of a β-type Ti-20Zr-40Ta alloy (TZT) was investigated. It was found that the recovery and the recrystallization occurred in the as-rolled alloy depended on the aging temperature. The periodically distributed Ta-lean phase (β1) and Ta-rich phase (β2) were produced by the spinodal decomposition in all the samples aged at different temperatures. The spinodal decomposition significantly influenced the mechanical properties and deformation mechanisms of the TZT alloy. Upon aging at 650 °C and 750 °C, the as-rolled alloy exhibited a double-yield phenomenon during tensile testing, indicating a stress-induced martensitic transformation; however, its ductility was limited due to the presence of ω phases. Conversely, aging at 850 °C resulted in an alloy with high strength and good ductility, which was potentially attributed to the enhanced strength resulting from modulated structures introduced with spinodal decomposition. [ABSTRACT FROM AUTHOR]
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- 2024
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10. Influence and Mechanism of the Excavation Width on Excavation Deformations in Shanghai Soft Clay.
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Huang, Pei, Dang, Kexin, Shi, Haili, Yang, Kun, and Wu, Jiacheng
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EXCAVATION ,RETAINING walls ,SHEARING force ,DEFORMATIONS (Mechanics) ,SOIL formation - Abstract
This study investigated the influence and mechanism of the excavation width on excavation deformations in Shanghai soft clay. Based on three excavations that had different final excavation depths, dissimilar retaining structures and diverse geological conditions, 40 sets of two-dimensional numerical models with different excavation widths were employed to analyze the deformation rules affected by the excavation width. Moreover, a series of simplified models with different excavation widths were employed to analyze the effect of the excavation width on excavation deformations. The results show that under the same excavation depth, both the horizontal displacements of the retaining walls and ground surface settlements increase as the excavation width increases, but the increasing rate gradually decreases. Factors such as the unloading influence depth, the overlap degree of the passive zones, the stress state of the basal soils and the development of the relative shear stress have a significant influence on excavation deformations. With increasing excavation width, the unloading influence depth gradually deepens, the overlap area of the passive zones gradually decreases, the direction of the rotation of the major principal stress gradually reduces and the relative shear stress of the distant and deep soils gradually expands. Therefore, the constraint ability of the passive zones on excavation deformation gradually reduces and excavation deformations gradually increase. [ABSTRACT FROM AUTHOR]
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- 2024
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11. Alterations in CX3CL1 Levels and Its Role in Viral Pathogenesis.
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Zhang, Chunmei, Zhang, Yusi, Zhuang, Ran, Yang, Kun, Chen, Lihua, Jin, Boquan, Ma, Ying, Zhang, Yun, and Tang, Kang
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HIV ,SARS-CoV-2 ,VIRUS diseases ,CHEMOKINE receptors ,CENTRAL nervous system ,ENCEPHALITIS ,INFLUENZA viruses ,FRACTALKINE ,CELL adhesion - Abstract
CX3CL1, also named fractalkine or neurotactin, is the only known member of the CX3C chemokine family that can chemoattract several immune cells. CX3CL1 exists in both membrane-anchored and soluble forms, with each mediating distinct biological activities. CX3CL1 signals are transmitted through its unique receptor, CX3CR1, primarily expressed in the microglia of the central nervous system (CNS). In the CNS, CX3CL1 acts as a regulator of microglia activation in response to brain disorders or inflammation. Recently, there has been a growing interest in the role of CX3CL1 in regulating cell adhesion, chemotaxis, and host immune response in viral infection. Here, we provide a comprehensive review of the changes and function of CX3CL1 in various viral infections, such as human immunodeficiency virus (HIV), SARS-CoV-2, influenza virus, and cytomegalovirus (CMV) infection, to highlight the emerging roles of CX3CL1 in viral infection and associated diseases. [ABSTRACT FROM AUTHOR]
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- 2024
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12. Dysregulated Glucuronidation of Bilirubin Exacerbates Liver Inflammation and Fibrosis in Schistosomiasis Japonica through the NF-κB Signaling Pathway.
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Xue, Qingkai, Wang, Yuyan, Liu, Yiyun, Hua, Haiyong, Zhou, Xiangyu, Xu, Yongliang, Zhang, Ying, Xiong, Chunrong, Liu, Xinjian, Yang, Kun, and Huang, Yuzheng
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HEPATIC fibrosis ,HEPATITIS ,SCHISTOSOMIASIS ,BILIRUBIN ,GLUCURONIDATION ,HEPATOTOXICOLOGY - Abstract
Hepatic fibrosis is an important pathological manifestation of chronic schistosome infection. Patients with advanced schistosomiasis show varying degrees of abnormalities in liver fibrosis indicators and bilirubin metabolism. However, the relationship between hepatic fibrosis in schistosomiasis and dysregulated bilirubin metabolism remains unclear. In this study, we observed a positive correlation between total bilirubin levels and the levels of ALT, AST, LN, and CIV in patients with advanced schistosomiasis. Additionally, we established mouse models at different time points following S. japonicum infection. As the infection time increased, liver fibrosis escalated, while liver UGT1A1 consistently exhibited a low expression, indicating impaired glucuronidation of bilirubin metabolism in mice. In vitro experiments suggested that SEA may be a key inhibitor of hepatic UGT1A1 expression after schistosome infection. Furthermore, a high concentration of bilirubin activated the NF-κB signaling pathway in L-O2 cells in vitro. These findings suggested that the dysregulated glucuronidation of bilirubin caused by S. japonicum infection may play a significant role in schistosomiasis liver fibrosis through the NF-κB signaling pathway. [ABSTRACT FROM AUTHOR]
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- 2024
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13. Estimation of Forest Canopy Fuel Moisture Content in Dali Prefecture by Combining Vegetation Indices and Canopy Radiative Transfer Models from MODIS Data.
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Yang, Kun, Tang, Bo-Hui, Fu, Wei, Zhou, Wei, Fu, Zhitao, and Fan, Dong
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FUELWOOD ,FOREST canopies ,RADIATIVE transfer ,MOISTURE ,FOREST fires ,FOREST fire prevention & control ,WILDFIRE prevention - Abstract
Forest canopy fuel moisture content (FMC) is a critical factor in assessing the vulnerability of a specific area to forest fires. The conventional FMC estimation method, which relies on look-up tables and loss functions, cannot to elucidate the relationship between FMC and simulated data from look-up tables. This study proposes a novel approach for estimating FMC by combining enhanced vegetation index (EVI) and normalized difference moisture index (NDMI). The method employs the PROSAIL + PROGeoSAIL two-layer coupled radiation transfer model to simulate the vegetation index, the water index, and the FMC value, targeting the prevalent double-layer structure in the study area's vegetation distribution. Additionally, a look-up table is constructed through numerical analysis to investigate the relationships among vegetation indices, water indices, and FMC. The results reveal that the polynomial equations incorporating vegetation and water indices as independent variables exhibit a strong correlation with FMC. Utilizing the EVI–NDMI joint FMC estimation method enables the direct estimation of FMC. The collected samples from Dali were compared with the estimated values, revealing that the proposed method exhibits superior accuracy (R2 = 0.79) in comparison with conventional FMC estimation methods. In addition, we applied this method to estimate the FMC in the Chongqing region one week before the 2022 forest fire event, revealing a significant decreasing trend in regional FMC leading up to the fire outbreak, highlighting its effectiveness in facilitating pre-disaster warnings. [ABSTRACT FROM AUTHOR]
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- 2024
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14. Evaluating the Effectiveness of the "River Chief System": An Empirical Study Based on the Water Quality Data of Coastal Rivers in Guangdong Province.
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Yang, Kun, Yao, Jinrui, Huang, Yin, Ling, Huiyan, Yang, Yu, Zhang, Lin, Chen, Diyun, and Liu, Yuxian
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WATER quality ,WATERSHEDS ,BIOCHEMICAL oxygen demand ,ENVIRONMENTAL health ,CHEMICAL oxygen demand - Abstract
The river chief system (RCS) is an innovative reform in China for strengthening the management of rivers and lakes. It is an important means of curbing the current severe water-environment situation. However, the policy impact of the RCS is still inconclusive in the existing literature. Using monthly data spanning from January 2015 to March 2022 from 25 water quality monitoring stations in rivers flowing into the sea across 13 prefecture-level cities in Guangdong Province, this study adopted regression discontinuity to evaluate the policy effects of the RCS on water quality. The results show that after the RCS's full implementation in Guangdong Province, the concentrations of dissolved oxygen (DO) increased and water quality indicators, such as permanganate index (COD
Mn ), biochemical oxygen demand (BOD), ammonia nitrogen (NH3 -N), chemical oxygen demand (COD), and total phosphorus (TP), decreased; NH3 -N showed the largest decrease. These findings indicate that the RCS may contribute to a measurable improvement in reducing water pollution. However, no statistically significant changes in pH and total nitrogen (TN) were found, which indicates that the RCS fell short of achieving the policy effect of comprehensive water-pollution control. Therefore, in order to improve the RCS, it is necessary to refine the existing water-quality assessment indicators and to establish an evaluation system centered on the ecological health of rivers and lakes. Additionally, a paradigm shift from an administrative-boundary-based river management model to an overarching, holistic river-basin-based management approach is crucial for actualizing the holistic governance goals of the RCS. [ABSTRACT FROM AUTHOR]- Published
- 2024
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15. Orientation Growth of N-Doped and Iron-Based Metal–Organic Framework and Its Application for Removal of Cr(VI) in Wastewater.
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Chen, Yan, Lei, Chao, Zhao, Yong-Gang, Ye, Ming-Li, and Yang, Kun
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METAL-organic frameworks ,DOPING agents (Chemistry) ,SEWAGE ,LANGMUIR isotherms ,AMMONIUM hydroxide - Abstract
A series of NH
2 -functionalized nano-sized magnetic metal–organic frameworks (MOFs) were prepared in this study for Cr(VI) removal from wastewater. It was observed that not only the morphological, i.e., orientation growth of N-doped and iron-based metal–organic frameworks, but also the adsorption of magnetic MOFs is largely related to the used amount of ammonium hydroxide in preparation. For example, with increasing amounts of ammonium hydroxide used in preparation, the morphology of magnetic MOFs changed from spherical to cube and triangular cone. Moreover, the maximum adsorption capacity of spherical-magnetic MOFs, cubic-magnetic MOFs and triangular cone-magnetic MOFs could be up to 204.08 mg/g, 232.56 mg/g and 270.27 mg/g, respectively. Under optimal conditions, the adsorption process of magnetic MOFs for Cr(VI) was consistent with the pseudo-second-order rate equation (R2 = 1) and Langmuir isotherm model (R2 > 0.99). Therefore, magnetic MOFs developed in this work offered a viable option for the removal of Cr(VI) from wastewater. [ABSTRACT FROM AUTHOR]- Published
- 2024
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16. The Effect of Water Content on Engine Oil Monitoring Based on Physical and Chemical Indicators.
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Zhou, Fanhao, Yang, Kun, and Wang, Ling
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INDICATORS & test-papers , *BASE oils , *ELECTRIC conductivity , *DIESEL motors , *INORGANIC acids , *ORGANIC acids - Abstract
Engine oil oxidation is one of the major reasons for oil aging which can result in variations in the physical and chemical properties of oil. Organic acids generated by oil oxidation can react with water to form inorganic acids and acidic substances (including organic and inorganic acids) that corrode engine parts, resulting in the generation of rust or damage to engine parts. This is one of the important reasons why oil should be regularly changed. One of the most commonly applied methods for judging the aging degree of engine oil is monitoring its acid number (AN). However, generally, the effect of oil water content on acid value measurement is not considered. When oils are used in engines, they are often contaminated by water due to condensation, which accelerates engine oil aging. Therefore, it is crucial to explore the water content effect on AN in the process of engine oil aging. In this research, a water content sensor was applied to characterize moisture content in oxidized oil samples. The sensor could also obtain oil sample electrical conductivity which corresponded to its dielectric constant. Using a mid-infrared spectrometer to measure oil sample AN at this point to obtain the variation in AN with oxidation time, oil sample AN was connected in series with the water content, dielectric constant and electrical conductivity. These parameters were monitored through sensors, and the effect of water content on AN was studied. Experimental results revealed that with the increase in oxidation time, the water content, electrical conductivity, dielectric constant increase and AN of oil were increased. At the same time, since the temperature had a greater effect on electrical conductivity, the application of an air-conditioned constant-temperature environment removed the effect of temperature change on electrical conductivity. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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17. High-Resolution Image Processing of Probe-Based Confocal Laser Endomicroscopy Based on Multistage Neural Networks and Cross-Channel Attention Module.
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Qiu, Yufei, Zhang, Haojie, Yang, Kun, Zhai, Tong, Lu, Yipeng, Cao, Zhongwei, and Zhang, Zhiguo
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LASERS ,FEATURE extraction ,EPITHELIUM ,DEEP learning ,SIGNAL-to-noise ratio ,IMAGE reconstruction - Abstract
Probe-based confocal laser endomicroscopy (pCLE) is a subcellular in vivo imaging technique that generates diagnostic images revealing malignant structural modifications in epithelial tissues. In the clinical diagnosis of probe confocal laser endomicroscopy (pCLE), the image background generally has the problems of dynamic blur or information loss, which is not conducive to achieving high-resolution and clear pCLE imaging. In recent years, deep learning technology has achieved remarkable results in image deblurring. For the task of recovering high-resolution pCLE images, the current methods still suffer from the following drawbacks: it is difficult to choose a strategy to make CNN converge at a deeper level and mainstream methods cannot handle the complex balance between spatial details and high-level feature information well when reconstructing clear images. In order to solve the problem, we propose a new cross-channel attention, multistage, high-resolution pCLE image deblurring structure. This methodology improves the supervised attention mechanism, enhances the ability of feature extraction and fusion capabilities, and improves the quality of image deblurring by adding cross-channel attention module (CAM) into the multistage neural networks' architecture. The experimental results show that the average peak signal-to-noise ratio (PSNR) of the proposed model on the dataset is as high as 29.643 dB, and the structural similarity (SSIM) reaches 0.855. This method is superior to the prior algorithms in the visualization of recovered images, and the edge and texture details of the restored pCLE images are clearer. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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18. Plasmon-Enhanced High-Order Harmonic Generation of Open-Ended Finite-Sized Carbon Nanotubes with Vacancy Defects.
- Author
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Hu, Qi, Yang, Kun, Li, Qiuju, Sun, Jin, and Ding, Zongling
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HARMONIC generation ,MAXWELL equations ,CARBON nanotubes ,ELECTRONIC excitation ,HYBRID systems ,MOLECULAR dynamics - Abstract
In this study, the plasmon-enhanced high-order harmonic generation (HHG) of H-terminated finite-sized armchair single-walled carbon nanotubes (SWCNTs) near Ag nanoparticles is investigated systematically. Multiscale methods that combine the real-time time-dependent Hartree–Fock (TDHF) approach at the semi-empirical intermediate neglected differential overlap (INDOS) Hamiltonian level for molecular electronic dynamics with the finite-difference time-domain (FDTD) and solving Maxwell's equations are used. It is found that for intact CNTs, HHG is significantly enhanced due to plasmon resonance. However, the nonlinear optical properties are saturated when the tube length increases enough in the inhomogeneous near-field. For long CNTs, the large gradient of a near-field is unfavorable for the nonlinear excitation of electrons. But defects can further change the properties of the spectra. The HHG of hybrid systems can be enhanced very clearly by introducing vacancy defects in CNTs. This enhancement is affected by the energy and intensity of the incident light, the near-field gradient, and the number and location of defects. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
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19. Measurement of Fitness and Predatory Ability of Four Predatory Mite Species in Tibetan Plateau under Laboratory Conditions.
- Author
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Xiang, Dong, Wang, Zhen, Xu, Long, Wang, Yunchao, Zhang, Huanhuan, and Yang, Kun
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PHYTOSEIIDAE ,PREDATORY mite ,ACARICIDES ,TWO-spotted spider mite ,SPIDER mites ,BIOLOGICAL pest control agents ,SPECIES - Abstract
Simple Summary: Two-spotted spider mites (Tetranychus urticae Koch, TSSMs) greatly harm vegetables and other crops in Tibet, but predatory mites are potential effective biological agents against them. Herein, we measure the fitness and predatory abilities of four predatory mite species in the Tibetan Plateau and confirm that the predatory mite Amblyseius swirskii (Athias-Henriot) (Acari: Phytoseiidae) had the highest fecundity, the highest pre-adult survival rate, and the highest predation capacity toward adult TSSMs at 15 d post-release. The results imply that A. swirskii is more effective for the control of TSSMs in laboratory conditions and can be viewed as an effective biological control agent candidate against TSSMs in Tibet. Predatory mites are biological control agents used in many countries against various vegetable pests, particularly spider mites. Despite the significant presence of predatory mites in the Tibetan plateau, there is limited research on their potential against spider mites in the area. This study investigated the fitness parameters and performance against TSSM of four predatory, including Amblyseius swirskii (Athias-Henriot) and three species from the genus Neoseiulus (Neoseiulus californicus (McGregor), Neoseiulus barkeri (Hughes), and Neoseiulus cucumeris (Oudemans)), originally collected from fields in the Tibetan Plateau. Compared to the other three predatory species, A. swirskii exhibited the highest fecundity (11.60 ± 0.34) and the highest pre-adult survival rate (83.33 ± 3.33%). Since their juvenile survival rate (SR) was extremely low (13.33% ± 5.77%), most N. barkeri nymphs died before emergence. Compared to the other three predatory mites, A. swirskii showed the highest predation capacity against adult TSSMs at 15 d post-release (14.28 ± 2.24). Based on the results, A. swirskii was the most effective, and N. barkeri was the least effective in controlling two-spotted mites in the Tibetan Plateau among the four species tested in this study. Collectively, these findings imply notable advantages in employing A. swirskii for controlling two-spotted mites in the Tibetan Plateau. This study informs the development of a feasible biological control method based on suitable predatory mite species to manage TSSMs in the Tibetan Plateau. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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- View/download PDF
20. 6- n -Butoxy-10-nitro-12,13-dioxa-11-azatricyclo[7.3.1.0 2,7 ]trideca-2,4,6,10-tetraene Improves the X-ray Sensitivity on Inhibiting Proliferation and Promoting Oxidative Stress and Apoptosis of Oral Cancer Cells.
- Author
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Yang, Kun-Han, Yen, Ching-Yu, Wang, Sheng-Chieh, Chang, Fang-Rong, Chang, Meng-Yang, Chan, Chieh-Kai, Jeng, Jiiang-Huei, Tang, Jen-Yang, and Chang, Hsueh-Wei
- Subjects
ORAL cancer ,CANCER cells ,OXIDATIVE stress ,REACTIVE oxygen species ,APOPTOSIS - Abstract
This in vitro study examines the anti-oral cancer effects and mechanisms of a combined X-ray/SK2 treatment, i.e., X-ray and 6-n-butoxy-10-nitro-12,13-dioxa-11-azatricyclo[7.3.1.0
2,7 ]trideca-2,4,6,10-tetraene (SK2). ATP cell viability and flow cytometry-based cell cycle, apoptosis, oxidative stress, and DNA damage assessments were conducted. The X-ray/SK2 treatment exhibited lower viability in oral cancer (Ca9-22 and CAL 27) cells than in normal (Smulow–Glickman, S-G) cells, i.e., 32.0%, 46.1% vs. 59.0%, which showed more antiproliferative changes than with X-ray or SK2 treatment. Oral cancer cells under X-ray/SK2 treatment showed slight subG1 and G2/M increments and induced high annexin V-monitored apoptosis compared to X-ray or SK2 treatment. The X-ray/SK2 treatment showed higher caspase 3 and 8 levels for oral cancer cells than other treatments. X-ray/SK2 showed a higher caspase 9 level in CAL 27 cells than other treatments, while Ca9-22 cells showed similar levels under X-ray and/or SK2. The X-ray/SK2 treatment showed higher reactive oxygen species (ROS) generation and mitochondrial membrane potential (MMP) depletion than other treatments. Meanwhile, the mitochondrial superoxide (MitoSOX) and glutathione levels in X-ray/SK2 treatment did not exhibit the highest rank compared to others. Moreover, oral cancer cells had higher γH2AX and/or 8-hydroxy-2-deoxyguanosine levels from X-ray/SK2 treatment than others. All these measurements for X-ray/SK2 in oral cancer cells were higher than in normal cells and attenuated by N-acetylcysteine. In conclusion, X-ray/SK2 treatment showed ROS-dependent enhanced antiproliferative, apoptotic, and DNA damage effects in oral cancer cells with a lower cytotoxic influence on normal cells. [ABSTRACT FROM AUTHOR]- Published
- 2024
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21. Thermal Deformation Behavior and Microstructural Evolution of Multicomponent Mg-Li-Zn-Al-Y Alloys under Hot Compression.
- Author
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Yang, Kun, Bai, Weiwu, Li, Bin, Chen, Hao, Li, Guo, Wei, Guobing, and Liu, Junwei
- Subjects
- *
ALLOYS , *DEFORMATIONS (Mechanics) , *GRAIN size , *STATISTICAL correlation - Abstract
High-temperature compression tests on Mg-11.5Li-2.5Zn-0.35Al-0.3Y (in wt.%) were carried out on a Gleeble-3500 thermal simulator. Flow stress and microstructural evolution were analyzed at different temperatures (T = 473 K, 523 K, 573 K, and 623 K) and strain rates ( ε ˙ = 1 s−1, 0.1 s−1, 0.01 s−1, and 0.001 s−1). On this basis, the constitutive model of the alloy was established using the Arrhenius-type constitutive model, and the thermal processing map of the alloy was drawn based on the DMM (dynamic material modeling) theory. The experimental results show that the flow stress of the Mg-11.5Li-2.5Zn-0.35Al-0.3Y alloy decreases with an increase in temperature and a decrease in strain rate. The grain size increases uniformly with the increase in temperature, while a sudden increase occurs with the decrease in strain rate. The predicted value of the model is compared with the experimental value to verify the correctness of the model, and the correlation coefficient, R = 0.9690, was calculated, which further proves the applicability of the model to the Mg-11.5Li-2.5Zn-0.35Al-0.3Y alloy. This alloy can be safely plastic-deformed 473 K~623 K and 0.001 s−1~1 s−1. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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22. Top-k Graph Similarity Search Algorithm Based on Chi-Square Statistics in Probabilistic Graphs.
- Author
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Chen, Ziyang, Zhuang, Junhao, Wang, Xuan, Tang, Xian, Yang, Kun, Du, Ming, and Zhou, Junfeng
- Subjects
SEARCH algorithms ,PATTERN recognition systems ,TABU search algorithm ,GRAPH labelings ,CHI-square distribution ,TELECOMMUNICATION systems - Abstract
Top-k graph similarity search on probabilistic graphs is widely used in various scenarios, such as symptom–disease diagnostics, community discovery, visual pattern recognition, and communication networks. The state-of-the-art method uses the chi-square statistics to speed up the process. The effectiveness of the chi-square statistics solution depends on the effectiveness of the sample observation and expectation. The existing method assumes that the labels in the data graphs are subject to uniform distribution and calculate the chi-square value based on this. In fact, however, the actual distribution of the labels does not meet the requirement of uniform distribution, resulting in a low quality of the returned results. To solve this problem, we propose a top-k similar subgraph search algorithm ChiSSA based on chi-square statistics. We propose two ways to calculate the expectation vector according to the actual distribution of labels in the graph, including the local expectation calculation method based on the vertex neighbors and the global expectation calculation method based on the label distribution of the whole graph. Furthermore, we propose two optimization strategies to improve the accuracy of query results and the efficiency of our algorithm. We conduct rich experiments on real datasets. The experimental results on real datasets show that our algorithm improves the quality and accuracy by an average of 1.66× and 1.68× in terms of time overhead, it improves by an average of 3.41×. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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23. An Overview on Fatigue of High-Entropy Alloys.
- Author
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Hu, Junchao, Li, Xue, Zhao, Qiuchen, Chen, Yangrui, Yang, Kun, and Wang, Qingyuan
- Subjects
ALLOY fatigue ,FATIGUE life ,FATIGUE crack growth ,MATERIAL fatigue ,CRACK propagation (Fracture mechanics) ,CRACK initiation (Fracture mechanics) ,MAGNETIC entropy - Abstract
Due to their distinct physical, chemical, and mechanical features, high-entropy alloys have significantly broadened the possibilities of designing metal materials, and are anticipated to hold a crucial position in key engineering domains such as aviation and aerospace. The fatigue performance of high-entropy alloys is a crucial aspect in assessing their applicability as a structural material with immense potential. This paper provides an overview of fatigue experiments conducted on high-entropy alloys in the past two decades, focusing on crack initiation behavior, crack propagation modes, and fatigue life prediction models. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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24. Mushrooms Adapted to Seawater: Two New Species of Candolleomyces (Basidiomycota, Agaricales) from China.
- Author
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Yang, Kun L., Lin, Jia Y., Li, Guang-Mei, and Yang, Zhu L.
- Subjects
- *
BASIDIOMYCOTA , *AGARICALES , *MARINE fungi , *SEAWATER , *MUSHROOMS - Abstract
Marine fungi have been studied for a long history in many realms, but there are few reports on marine mushrooms. In this study, marine fungi with conspicuous subglobose sequestrate basidioma were discovered from mangrove forests in South China. They grow on the deadwood of mangroves in the intertidal zone, periodically submerging into seawater due to the tide. Some marine animals were observed to nest in their basidiomata or consume them as food. The pileus-gleba-inner veil complex (PGI) of the basidioma was observed to be detached from the stipe and transferred into seawater by external forces, and drifting on sea to spread spores after maturity. The detachment mechanism of their PGIs was revealed through detailed microscopic observations. The contrast culturing experiment using freshwater and seawater potato dextrose agar media showed they have probably obligately adapted to the marine environment. Based on morphological and molecular phylogenetic evidence, two new species of Candolleomyces (Basidiomycota, Agaricales), namely C. brunneovagabundus and C. albovagabundus, were described. They are similar and close to each other, but can be distinguished by the size and color of the basidioma, and the size of the basidiospores. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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25. Precision Isolation of Circulating Leukemia Cells in Chronic Myelogenous Leukemia Patients Using a Novel Microfluidic Device and Its Clinical Applications.
- Author
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Ouyang, Dongfang, Ye, Ningxin, Yang, Kun, Wang, Yiyang, Hu, Lina, Chao, Shuen, Toner, Mehmet, and Li, Yonghua
- Subjects
BIOMARKERS ,FLOW cytometry ,CONFIDENCE intervals ,CHRONIC myeloid leukemia ,INDIVIDUALIZED medicine ,DESCRIPTIVE statistics ,RESEARCH funding ,MICROFLUIDICS ,CELL lines ,LONGITUDINAL method - Abstract
Simple Summary: Chronic Myelogenous Leukemia (CML) is a common blood cancer that involves the uncontrolled growth of certain blood cells. Treating this disease effectively requires doctors to detect and eliminate even the smallest traces of cancer cells, a process known as managing minimal residual disease. However, current methods to find these cells, like bone marrow tests, are not only uncomfortable for patients but also have some drawbacks, such as missing cancer cells. This study introduces an innovative, less invasive approach using a microfluidic chip. This chip works by analyzing a patient's blood sample to find and measure the number of leukemia cells. We tested this device on 56 patients with CML and found that it could successfully identify these cancer cells in blood, with a high accuracy rate. This new method is promising because it is less invasive and more precise, making it easier to monitor how well a patient's treatment is working and to make better treatment decisions. This advancement could significantly improve the care and outcomes of people with CML, making a real difference in their lives. Chronic Myelogenous Leukemia (CML) is a prevalent hematologic malignancy characterized by the malignant transformation of myeloid cells and their proliferation in the peripheral blood. The management of CML poses significant challenges, particularly in detecting and eradicating minimal residual disease, which is crucial for preventing relapse and improving survival outcomes. Traditional minimal residual disease detection methods, such as bone marrow aspiration, are invasive and have limitations which include the potential for sampling errors and false negatives. This study introduces a novel label-free microfluidic chip designed for the segregation and recovery of circulating leukemia cells, offering a non-invasive liquid biopsy approach with potential applications in precision medicine. Over July 2021 to October 2023, we recruited 56 CML patients across various disease stages and collected blood samples for analysis using our microfluidic device. The device demonstrated high efficacy in isolating circulating leukemia cells, with an optimal capture efficiency of 78% at a sample flow rate of 3 mL/h. Our results indicate that the microfluidic device can efficiently segregate and quantify circulating leukemia cells, providing a detailed understanding of CML progression and treatment response. The significant reduction in circulating leukemia cell counts in patients in complete remission highlights the device's potential in monitoring treatment efficacy. Furthermore, the device's sensitivity in detecting minimal residual disease could offer a more reliable prognostic tool for therapeutic decision-making in CML management. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
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26. Responses of Vegetation Phenology to Urbanization in Plateau Mountains in Yunnan, China.
- Author
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Sun, Mengzhu, Yang, Kun, Wang, Jiasheng, Ran, Wenjing, and Rao, Xun
- Subjects
PLANT phenology ,PHENOLOGY ,URBANIZATION ,GROWING season ,VEGETATION dynamics ,URBAN planning - Abstract
The process of urbanization alters the distribution of land use and gives rise to certain climatic modifications that have a direct influence on vegetation phenology. Mountainous areas have a fragile biological environment, and vegetation phenology is relatively sensitive to urbanization. However, there is a paucity of research on the impact of urbanization in mountainous areas on vegetation phenology. The Urban Agglomeration of Central Yunnan (UACY) is located on the Yunnan–Guizhou Plateau in China. This study explored the vegetation phenological changes in different terrains from 2001 to 2020 in the UACY based on remote sensing data. Using the dynamic gradient method, we examined the response of vegetation phenology to urbanization from three aspects: urban–rural gradient, urbanization intensity (UI), and population density. The results showed that landform has a significant impact on SOS (start of growing season), with a topographic relief difference of 200 m acting as the dividing line. The findings derived from the dynamic gradient analysis indicate that UI has the most significant effect. SOS advances by 5.77 days (R
2 = 0.96), EOS (end of growing season) advances by 2.30 days (R2 = 0.83), and LOS (length of growing season) lengthens by 2.59 days (R2 = 0.87) for every 10% increase in UI. This study has the potential to serve as a valuable resource for future urban planning and administration in the UACY. Additionally, it could provide decision-making support for the development of mountainous urban agglomerations in ecological environments. [ABSTRACT FROM AUTHOR]- Published
- 2023
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27. Knowledge Distillation for Enhancing a Lightweight Magnet Tile Target Detection Model: Leveraging Spatial Attention and Multi-Scale Output Features.
- Author
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Huang, Qinyuan, Yang, Kun, Zhu, Yuzhen, Chen, Long, and Cao, Lijia
- Subjects
PROCESS capability ,TILES ,FEATURE extraction ,COMPUTER vision ,CERAMIC tiles ,COMPUTER algorithms ,MAGNETS - Abstract
Accurate and efficient sorting of diverse magnetic tiles during manufacturing is vital. However, challenges arise due to visual similarities among types, necessitating complex computer vision algorithms with large sizes and high computational needs. This impedes cost-effective deployment in the industry, resulting in the continued use of inefficient manual sorting. To address this issue, we propose an innovative lightweight magnetic tile detection approach that improves knowledge distillation for a compressed YOLOv5s model. Incorporating spatial attention modules into different feature extraction stages of YOLOv5s during the knowledge distillation process can enhance the ability of the compressed model to learn the knowledge of intermediate feature extraction layers from the original large model at different stages. Combining different outputs to form a multi-scale output, the multi-scale output feature in the knowledge refinement process enhances the capacity of the compressed model to grasp comprehensive target knowledge in outputs. Experimental results on our self-built magnetic tile dataset demonstrate significant achievements: 0.988 mean average precision, 0.5% discrepancy compared to the teacher's network, and an 85% model size reduction. Moreover, a 36.70% boost in inference speed is observed for single image analysis. Our method's effectiveness is also validated by the Pascal VOC dataset results, showing potential for broader target detection scenarios. This approach offers a solution to magnetic tile target detection challenges while being expected to expand to other applications. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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28. Study of Transcriptomic Analysis of Yak (Bos grunniens) and Cattle (Bos taurus) Pulmonary Artery Smooth Muscle Cells under Oxygen Concentration Gradients and Differences in Their Lung Histology and Expression of Pyruvate Dehydrogenase Kinase 1-Related Factors
- Author
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Zhang, Yiyang, Zhou, Manlin, Liang, Yuxin, Li, Rui, Zhang, Lan, Chen, Shuwu, Yang, Kun, Ding, Haie, Tan, Xiao, Zhang, Qian, and Qiao, Zilin
- Subjects
PYRUVATE dehydrogenase kinase ,CATTLE ,YAK ,LUNGS ,GENE expression ,PULMONARY artery ,PYRUVATE kinase ,VASCULAR endothelial growth factors - Abstract
Simple Summary: The lung is a key organ that exhibits adaptive changes in response to high altitude in mammals. The prolonged presence of lowland cattle at high altitude leads to the abnormal proliferation of pulmonary vascular smooth muscle cells, resulting in pulmonary vascular remodeling, whereas the underlying molecular mechanisms in yaks, a representative model for mammalian high-altitude acclimatization studies, remain unknown. In this manuscript, we investigated the transcriptomic analysis of yak and cattle pulmonary artery smooth muscle cells in vitro at different oxygen concentrations (1%, 10%, and 20%) and in vivo to observe the lung tissue structure as well as the distribution of PDK1, HIF-1α, and VEGF and differences in their expression in the lungs of plateau yaks and plains cattle. The results showed that the HIF-1 signaling pathway, glucose metabolism pathway, and related factors (HK2/PGK1/ALDOA/ALDH1A3/EHHADH) were closely related to each other, that there were obvious differences between yak lung tissues and those of plains cattle, and that there might be a regulatory relationship between the differences in the distribution and expression of PDK1, HIF-1α, and VEGF and the adaptation of yak lungs to the plateau hypoxic environment. The differences in the distribution and expression of PDK1, HIF-1α, and VEGF may be related to the adaptation of yak lungs to the plateau hypoxic environment, which provides basic information for studying the mechanism of hypoxia adaptation in yaks. The aim of this study was to investigate the molecular mechanisms by which hypoxia affects the biological behavior of yak PASMCs, the changes in the histological structure of yak and cattle lungs, and the relationships and regulatory roles that exist regarding the differences in the distribution and expression of PDK1 and its hypoxia-associated factors screened for their role in the adaptation of yak lungs to the plateau hypoxic environment. The results showed that, at the level of transcriptome sequencing, the molecular regulatory mechanisms of the HIF-1 signaling pathway, glucose metabolism pathway, and related factors (HK2/PGK1/ENO1/ENO3/ALDOC/ALDOA) may be closely related to the adaptation of yaks to the hypoxic environment of the plateau; at the tissue level, the presence of filled alveoli and semi-filled alveoli, thicker alveolar septa and basement membranes, a large number of erythrocytes, capillary distribution, and collagen fibers accounted for all levels of fine bronchioles in the lungs of yaks as compared to cattle. A higher percentage of goblet cells was found in the fine bronchioles of yaks, and PDK1, HIF-1α, and VEGF were predominantly distributed and expressed in the monolayers of ciliated columnar epithelium in the branches of the terminal fine bronchioles of yak and cattle lungs, with a small amount of it distributed in the alveolar septa; at the molecular level, the differences in PDK1 mRNA relative expression in the lungs of adult yaks and cattle were not significant (p > 0.05), the differences in HIF-1α and VEGF mRNA relative expression were significant (p < 0.05), and the expression of PDK1 and HIF-1α proteins in adult yaks was stronger than that in adult cattle. PDK1 and HIF-1α proteins were more strongly expressed in adult yaks than in adult cattle, and the difference was highly significant (p < 0.01); the relative expression of VEGF proteins was not significantly different between adult yaks and cattle (p > 0.05). The possible regulatory relationship between the above results and the adaptation of yak lungs to the plateau hypoxic environment paves the way for the regulatory mechanisms of PDK1, HIF-1α, and VEGF, and provides basic information for studying the mechanism of hypoxic adaptation of yaks in the plateau. At the same time, it provides a reference for human hypoxia adaptation and a target for the prevention and treatment of plateau diseases in humans and plateau animals. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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29. Environmental Cadmium Exposure Perturbs Gut Microbial Dysbiosis in Ducks.
- Author
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Wang, Xuefei, Mi, Junxian, Yang, Kun, and Wang, Lian
- Subjects
CADMIUM poisoning ,CADMIUM ,ENVIRONMENTAL exposure ,GUT microbiome ,HEAVY metal toxicology ,DUCKS ,DYSBIOSIS - Abstract
Simple Summary: Cadmium is a prevalent heavy metal known to contribute to environmental contamination. Moreover, cadmium in the environment may accumulate in animals and plants and then enter the human body through the food chain, seriously threatening food safety and public health. Currently, cadmium exposure has been demonstrated to cause liver and kidney damage, as well as abnormal bone development, in ducks. However, there is limited research on the impact of cadmium on the gut microbiota of ducks. In this study, we investigated the alterations in the gut microbiota of ducks exposed to cadmium and identified the detrimental effects of cadmium on the gut microbiota. Ore extraction, chemical production, and agricultural fertilizers may release significant amounts of heavy metals, which may eventually accumulate widely in the environment and organisms over time, causing global ecological and health problems. As a recognized environmental contaminant, cadmium has been demonstrated to cause osteoporosis and renal injury, but research regarding the effects of cadmium on gut microbiota in ducks remains scarce. Herein, we aimed to characterize the adverse effects of cadmium on gut microbiota in ducks. Results indicated that cadmium exposure dramatically decreased gut microbial alpha diversity and caused significant changes in the main component of gut microbiota. Moreover, we also observed significant changes in the gut microbial composition in ducks exposed to cadmium. A microbial taxonomic investigation showed that Firmicutes, Bacteroidota, and Proteobacteria were the most preponderant phyla in ducks regardless of treatment, but the compositions and abundances of dominant genera were different. Meanwhile, a Metastats analysis indicated that cadmium exposure also caused a distinct increase in the levels of 1 phylum and 22 genera, as well as a significant reduction in the levels of 1 phylum and 36 genera. In summary, this investigation demonstrated that cadmium exposure could disturb gut microbial homeostasis by decreasing microbial diversity and altering microbial composition. Additionally, under the background of the rising environmental pollution caused by heavy metals, this investigation provides a crucial message for the assessment of environmental risks associated with cadmium exposure. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
30. Heat-Flow Coupling Law for Freezing a Pipe Reinforcement with Varying Curvatures.
- Author
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Yang, Kun, Hu, Jun, and Wang, Tao
- Subjects
DARCY'S law ,FREEZING ,FREEZE-thaw cycles ,CURVATURE ,BURIED pipes (Engineering) ,PIPE - Abstract
Using the temperature and seepage field-coupling module within COMSOL Multiphysics software, we examined freezing behavior and its evolving patterns in curved underground freezing pipes. This study employed transient states, with the Darcy's law and porous-media heat-transfer options activated in the Physical Field Interface of the Physical Field and Variable Selection column. The models were created to establish numerical models of freezing reinforcement for both single and multiple pipes with various curvatures. These models were designed to simulate the evolving temperature and seepage fields of soil under diverse freezing conditions. Subsequently, this research utilized the models to simulate the freezing and consolidation conditions of a shallowly buried tunnel within the context of shallow tunnel conditions. The study reveals that after freezing a single pipe using water flow, the change in thickness of the frozen wall in curved pipes is notably smaller than that in straight pipes. This difference is particularly pronounced in the upstream section. Specifically, at a distance of −2000 mm from the main surface, the change in thickness of the frozen wall in straight pipes exceeds that in s = 7 curved pipes by approximately 350 mm. The smaller the long arc ratio s, the greater the arc of the freezing tube and the better the water-blocking effect. In the multi-pipe freezing model, the s = 7 curved pipes exhibit a frozen-wall thickness approximately 120 mm greater than that of straight pipes at a distance of −2000 mm from the main surface. Under the condition of a shallow buried concealed excavation with surging water, a pipe with a long arc ratio s = 7 arc freezing at 46 d attains a permafrost curtain thickness that is equivalent to that achieved by the straight pipe freezing at 58 d. This reduction in thickness shortens the working period by 12 days, resulting in a more efficient process. The successful application of the freezing method in the water-rich aquifer is expected to be a valuable reference for similar projects in the future. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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- View/download PDF
31. In Silico Analyses, Experimental Verification and Application in DNA Vaccines of Ebolavirus GP-Derived pan-MHC-II-Restricted Epitopes.
- Author
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Zhang, Junqi, Sun, Baozeng, Shen, Wenyang, Wang, Zhenjie, Liu, Yang, Sun, Yubo, Zhang, Jiaxing, Liu, Ruibo, Wang, Yongkai, Bai, Tianyuan, Ma, Zilu, Luo, Cheng, Qiao, Xupeng, Zhang, Xiyang, Yang, Shuya, Sun, Yuanjie, Jiang, Dongbo, and Yang, Kun
- Subjects
DNA vaccines ,EPITOPES ,EBOLA virus disease ,EBOLA virus ,HLA histocompatibility antigens ,BACTERIAL cell surfaces - Abstract
(1) Background and Purpose: Ebola virus (EBOV) is the causative agent of Ebola virus disease (EVD), which causes extremely high mortality and widespread epidemics. The only glycoprotein (GP) on the surface of EBOV particles is the key to mediating viral invasion into host cells. DNA vaccines for EBOV are in development, but their effectiveness is unclear. The lack of immune characteristics resides in antigenic MHC class II reactivity. (2) Methods: We selected MHC-II molecules from four human leukocyte antigen II (HLA-II) superfamilies with 98% population coverage and eight mouse H2-I alleles. IEDB, NetMHCIIpan, SYFPEITHI, and Rankpep were used to screen MHC-II-restricted epitopes with high affinity for EBOV GP. Further immunogenicity and conservation analyses were performed using VaxiJen and BLASTp, respectively. EpiDock was used to simulate molecular docking. Cluster analysis and binding affinity analysis of EBOV GP epitopes and selected MHC-II molecules were performed using data from NetMHCIIpan. The selective GP epitopes were verified by the enzyme-linked immunospot (ELISpot) assay using splenocytes of BALB/c (H2d), C3H, and C57 mice after DNA vaccine pVAX-GP
EBO immunization. Subsequently, BALB/c mice were immunized with Protein-GPEBO , plasmid pVAX-GPEBO , and pVAX-LAMP/GPEBO , which encoded EBOV GP. The dominant epitopes of BALB/c (H-2-I-AdEd genotype) mice were verified by the enzyme-linked immunospot (ELISpot) assay. It is also used to evaluate and explore the advantages of pVAX-LAMP/GPEBO and the reasons behind them. (3) Results: Thirty-one HLA-II-restricted and 68 H2-I-restricted selective epitopes were confirmed to have high affinity, immunogenicity, and conservation. Nineteen selective epitopes have cross-species reactivity with good performance in MHC-II molecular docking. The ELISpot results showed that pVAX-GPEBO could induce a cellular immune response to the synthesized selective peptides. The better immunoprotection of the DNA vaccines pVAX-LAMP/GPEBO coincides with the enhancement of the MHC class II response. (4) Conclusions: Promising MHC-II-restricted candidate epitopes of EBOV GP were identified in humans and mice, which is of great significance for the development and evaluation of Ebola vaccines. [ABSTRACT FROM AUTHOR]- Published
- 2023
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32. Coupling of Pyro–Piezo-Phototronic Effects in a GaN Nanowire.
- Author
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Qin, Guoshuai, Wang, Zhenyu, Wang, Lei, Yang, Kun, Zhao, Minghao, and Lu, Chunsheng
- Subjects
NANOWIRES ,GALLIUM nitride ,ELECTRIC fields ,OPTOELECTRONIC devices ,ULTRAVIOLET radiation - Abstract
In this paper, we systematically investigate the synergistic regulation of ultraviolet and mechanical loading on the electromechanical behavior of a GaN nanowire. The distributions of polarization charge, potential, carriers, and electric field in the GaN nanowire are analytically represented by using a one-dimensional model that combines pyro-phototronic and piezo-phototronic properties, and then, the electrical transmission characteristics are analyzed. The results suggest that, due to the pyro-phototronic effect and ultraviolet photoexcited non-equilibrium carriers, the electrical behavior of a nano-Schottky junction can be modulate by ultraviolet light. This provides a new method for the function improvement and performance regulation of intelligent optoelectronic nano-Schottky devices. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
33. Neuromuscular Magnetic Field Measurement Based on Superconducting Bio-Sensors.
- Author
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Zhang, Zhidan, He, Anran, Xu, Zihan, Yang, Kun, and Kong, Xiangyan
- Subjects
MAGNETIC field measurements ,SUPERCONDUCTING quantum interference devices ,MAGNETIC sensors ,NEUROMUSCULAR diseases ,MAGNETIC fields ,RESEARCH personnel - Abstract
These years, disease-causing and disabling diseases have caused great concern. Neurological musculoskeletal disorders are diverse and affect people of a wide range of ages. And the lack of comprehensive diagnostic methods places a huge burden on healthcare systems and social economies. In this paper, the current status of clinical research on neuromuscular diseases is introduced, and the advantages of magnetic field measurement compared with clinical diagnostic methods are illustrated. A comprehensive description of the related technology of superconducting quantum interference devices (SQUIDs), magnetic field detection noise suppression scheme, the development trend of the sensor detection system, and the application and model establishment of the neuromuscular magnetic field is also given in this paper. The current research and development trends worldwide are compared simultaneously, and finally the conclusions and outlook are put forward. Based on the description of the existing literature and the ideas of other researchers, the next development trends and my own research ideas are presented in this paper, that is, starting from the establishment of a neuromuscular model, combining medical and industrial work, designing a sensor system that meets clinical needs, and laying the foundation for the clinical application of a bio-magnetic system. This review promotes a combination between medicine and industry, and guides researchers on considering the challenges of sensor development in terms of clinical needs. In addition, in this paper, the development trends are described, including the establishment of the model, the clinical demand for sensors, and the challenges of system development so as to give certain guidance to researchers. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
34. Monodisperse Fluorescent Polystyrene Microspheres for Staphylococcus aureus Aerosol Simulation.
- Author
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Lu, Siyu, Li, Fan, Liu, Bo, Yang, Kun, Tian, Feng, Cheng, Zhi, Ding, Sheng, and Hou, Kexin
- Subjects
MICROSPHERES ,STAPHYLOCOCCUS aureus ,AEROSOLS ,POLYSTYRENE ,PARTICLE size distribution ,DUST ,POLYMETHYLMETHACRYLATE - Abstract
Staphylococcus aureus (SA) is one of the most common causes of hospital-acquired infections and foodborne illnesses and is commonly found in nature in air, dust, and water. The spread and transmission of SA aerosols in the air has the potential to cause epidemic transmission among humans and between humans and animals. To effectively provide the timely warning of SA aerosols in the atmosphere, the identification and detection of SA aerosol concentrations are required. Due to their homogeneous physicochemical properties, highly monodisperse submicron polystyrene (PS) microspheres can be used as one of the simulants of SA aerosols. In this study, 800 nm monodisperse fluorescent PS (f-PS) microspheres with fluorescence spectra and particle size distribution similar to those of SA were prepared. The 800 nm monodisperse f-PS microspheres had a fluorescence characteristic peak at 465 nm; in aerosols, 800 nm monodisperse f-PS microspheres with a similar particle size distribution to that of SA were further verified, mainly in the range of 500 nm–1000 nm; finally, it was found that the f-PS microspheres still possessed similar fluorescence characteristics after 180 days. The f-PS microspheres prepared in this study are very close to SA in terms of particle size and fluorescence properties, providing a new idea for aerosol analogs of SA. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
35. Evaluation of Tropical Cyclone Disaster Loss Using Machine Learning Algorithms with an eXplainable Artificial Intelligence Approach.
- Author
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Liu, Shuxian, Liu, Yang, Chu, Zhigang, Yang, Kun, Wang, Guanlan, Zhang, Lisheng, and Zhang, Yuanda
- Abstract
In the context of global warming, tropical cyclones (TCs) have garnered significant attention as one of the most severe natural disasters in China, particularly in terms of assessing the disaster losses. This study aims to evaluate the TC disaster loss (TCDL) using machine learning (ML) algorithms and identify the impact of specific feature factors on the prediction of model with an eXplainable Artificial Intelligence (XAI) approach, SHapley Additive exPlanations (SHAP). The results show that LightGBM outperforms Random Forest (RF), Support Vector Machine (SVM), and Naive Bayes (NB) for estimating the TCDL grades, achieving the highest accuracy value of 0.86. According to the SHAP values, the three most important factors in the LightGBM classifier model are proportion of stations with rainfall exceeding 50 mm (ProRain), maximum wind speed (MaxWind), and maximum daily rainfall (MaxRain). Specifically, in the estimation of high TCDL grade, events characterized with MaxWind exceeding 30 m/s, MaxRain exceeding 200 mm, and ProRain exceeding 30% tend to exhibit a higher susceptibility to TC disaster due to positive SHAP values. This study offers a valuable tool for decision-makers to develop scientific strategies in the risk management of TC disaster. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
36. Microstructure Characteristics, Mechanical Properties and Strain Hardening Behavior of B2 Intermetallic Compound-Strengthening Fe-16Mn-9Al-0.8C-3Ni Steel Fabricated by Twin-Roll Strip Casting, Cold Rolling and Annealing.
- Author
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Zhang, Baoguang, Yang, Kun, Zhang, Xiaoming, Liu, Haitao, Zhang, Weina, and Wang, Jian
- Subjects
- *
COLD rolling , *STRAINS & stresses (Mechanics) , *STRAIN hardening , *LIGHTWEIGHT steel , *TENSILE strength , *LIGHTWEIGHT materials - Abstract
In this study, the Fe-16Mn-9Al-0.8C-3Ni (wt.%) lightweight steel was fabricated by novel twin-roll strip casting technology. The microstructure, tensile properties and strain-hardening behavior of the present steel have been investigated and compared to those of conventionally processed steels with similar chemical compositions. After annealing, a unique gradient microstructure of intermetallic compound (B2)-austenite was obtained along the thickness direction, consisting of granular B2 (average: 430 nm) and fine austenite (average: 1.82 μm) at the surface layer, blocky B2 (average: 1.03 μm) and medium austenite (average: 3.98 μm) at the quarter layer and polygonal B2 (average: 1.94 μm) and coarse austenite (average: 6.13 μm) at the center layer. The cooperative action of B2 pinning dislocation, plane slip and back stress led to stronger strain hardening, among which the strong back stress effect originated from the multistage discontinuous austenite deformation and the mechanical incompatibility between austenite and B2 is believed to be the most important reason, thereby achieving an excellent balance of strength (ultimate tensile strength: 1147 MPa) and ductility (total elongation: 43.2%). This work not only developed a new processing way to fabricate Ni-containing Fe-Mn-Al-C lightweight steel with outstanding mechanical properties, but also provided a potential solution for manufacturing some other metallic materials accompanied by brittle B2 intermetallic. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
37. Comparing Mechanical Characterization of Carbon, Kevlar, and Hybrid-Fiber-Reinforced Concrete under Quasistatic and Dynamic Loadings.
- Author
-
Li, Yeou-Fong, Yang, Kun-Han, Hsu, Pei-Yao, Syu, Jin-Yuan, Wang, Shea-Jue, Kuo, Wen-Shyong, and Tsai, Ying-Kuan
- Subjects
POLYPHENYLENETEREPHTHALAMIDE ,DYNAMIC loads ,BRITTLE materials ,CONCRETE ,FIBER-reinforced concrete ,CARBON fibers - Abstract
Concrete is a brittle material due to its poor tensile strength; consequently, concrete tends to crack or peel under an applied external load. Previous studies have investigated the effect of incorporating fiber into concrete, which can improve its tensile strength. In this study, the static and dynamic mechanical characteristics of three types of fiber-reinforced concrete (FRC) were examined: carbon-fiber-reinforced concrete (CFRC); Kevlar-fiber-reinforced concrete (KFRC); and a combination of both, known as carbon/Kevlar-hybrid-fiber-reinforced concrete (HFRC). This study created concrete specimens by pneumatically dispersing carbon and Kevlar fibers and mixing them with cement to comprise 1% of the weight. The mixture was then combined with aggregates and water to form the concrete specimens. When compared with the benchmark concrete specimens, it was found that the compressive strength of the CFRC, KFRC, and HFRC specimens increased by about 19% to 50%, the bending strength increase by about 8% to 32%, and the splitting strength increased by about 4% to 36%. Specifically, the HFRC made with the 24 mm carbon and Kevlar fibers displayed the most significant mechanical strength in a static state. Furthermore, the HFRC showed superior resistance to impact compared to the benchmark concrete specimens across various impact energies, with the 24 mm carbon and Kevlar fiber HFRC showing the highest resistance. The inclusion of fibers in the split Hopkinson pressure bar (SHPB) test demonstrated a notable increase in the maximum strength, particularly in the case of the 12 mm carbon fiber combined with the 24 mm Kevlar fiber in the HFRC specimen. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
38. Fabrication of High Aspect Ratio Nano-Channels by Thermal Nano-Imprinting and Parylene Deposition.
- Author
-
Yang, Kun, Yin, Zhifu, and Sun, Lei
- Subjects
PHOTORESISTS - Abstract
A low-cost method of fabrication of high aspect ratio nano-channels by thermal nano-imprinting and Parylene deposition is proposed. SU-8 photoresist nano-channels were first manufactured by thermal nano-imprinting, and Parylene deposition was carried out to reduce the width of the nano-channels and increase the aspect ratio. During the process, the side walls of the SU-8 nano-channels were covered with the Parylene film, reducing the width of the nano-channels, and the depth of the channels increased due to the thickness of the Parylene film deposited on the surface of the SU-8 nano-channels, more so than that at the bottom. The influence of Parylene mass on the size of nano-channels was studied by theoretical analysis and experiments, and the deposition pressure of Parylene was optimized. The final high aspect ratio nano-channels are 46 nm in width and 746 nm in depth, of which the aspect ratio is 16. This simple and efficient method paves the way for the production of high aspect ratio nano-channels. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
39. Self-Powered, Non-Toxic, Recyclable Thermogalvanic Hydrogel Sensor for Temperature Monitoring of Edibles.
- Author
-
Yang, Kun, Bai, Chenhui, Liu, Boyuan, Liu, Zhoutong, and Cui, Xiaojing
- Subjects
TEMPERATURE sensors ,ELECTRICAL energy ,OPEN-circuit voltage ,SHORT-circuit currents ,POLYMER networks ,BLOOD sugar monitoring ,COLD (Temperature) ,POLYMER colloids - Abstract
Thermogalvanic hydrogel, an environmentally friendly power source, enable the conversion of low-grade thermal energy to electrical energy and powers microelectronic devices in a variety of scenarios without the need for additional batteries. Its toxicity, mechanical fragility and low output performance are a hindrance to its wide application. Here, we demonstrate thermoelectric gels with safe non-toxic, recyclable, highly transparent and flexible stretchable properties by introducing gelatin as a polymer network and SO 3 / 4 2 − as a redox electric pair. When the temperature difference is 10 K, the gel-based thermogalvanic cell achieves an open-circuit voltage of about 16.2 mV with a maximum short-circuit current of 39 μ A. Furthermore, we extended the application of the Gel-SO 3 / 4 2 − gel to monitor the temperature of hot or cold food, enabling self-powered sensing for food temperature detection. This research provides a novel concept for harvesting low-grade thermal energy and achieving safe and harmless self-driven temperature monitoring. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
40. Serum Malondialdehyde-Oxidized Low-Density Lipoprotein Level Is Associated with Arterial Stiffness by Cardio-Ankle Vascular Index in Coronary Artery Bypass Graft Patients.
- Author
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Hung, Ting, Jhan, Jin-You, Lin, Jian-Hong, Yang, Kun-Ta, Hsu, Bang-Gee, and Chang, Jui-Chih
- Subjects
ANKLE brachial index ,CORONARY artery bypass ,ARTERIAL diseases ,MAJOR adverse cardiovascular events ,ENZYME-linked immunosorbent assay ,LOGISTIC regression analysis - Abstract
A high malondialdehyde-oxidized low-density lipoprotein (MDA-oxLDL) level is associated with atherosclerotic cardiovascular diseases and major adverse cardiovascular events. A higher cardio-ankle vascular index (CAVI) is independently associated with an increased risk of cardiovascular events, cardiovascular mortality, myocardial infarction, and stroke in patients with cardiovascular risk. Thus, this study aimed to evaluate the relationship between serum MDA-oxLDL levels and CAVI in patients with triple-vessel coronary artery disease who underwent coronary artery bypass graft (CABG) surgery. Fasting blood samples and baseline characteristics were obtained from 88 patients who had undergone CABG. A commercialized enzyme-linked immunosorbent assay was used to measure MDA-oxLDL levels. An automatic pulse wave analyzer was used to measure CAVI values, and each side of CAVI values of ≥9 was designated as arterial stiffness. In total, 47 participants were assigned to the arterial stiffness group. More patients had diabetes mellitus, were older, and had higher serum MDA-oxLDL levels in the arterial stiffness group than in the control group. A multivariate logistic regression analysis disclosed that MDA-oxLDL and diabetes mellitus were independent predictors of arterial stiffness. Moreover, according to the Spearman's correlation analysis, the serum MDA-oxLDL level was positively associated with both left and right CAVI. Serum MDA-oxLDL levels were positively associated with arterial stiffness in patients who had undergone CABG. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
41. A Theoretical Investigation of the Structural and Electronic Properties of P/SnBr 2 Heterojunctions.
- Author
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Yang, Kun, Ding, Zongling, Hu, Qi, Sun, Jin, and Li, Qiuju
- Subjects
DENSITY functionals ,DENSITY functional theory ,ELECTRIC fields ,HETEROJUNCTIONS - Abstract
In this paper, the structural and electronic properties of P/SnBr
2 heterojunctions were investigated using the first-principles calculation method based on the density functional theory (DFT). The band alignment of the P/SnBr2 heterojunction was type I. The bandgap value was 0.71 eV in the DFT calculation. Furthermore, the bandgap of the heterojunction could be efficiently tuned by controlling an electric field and biaxial strain. The bandgap changed linearly with the electric field in a certain range; when the electric field was greater than 0.8 V/Å, the heterojunction was metallic. The bandgap could also be tuned when a biaxial strain was applied. Under tensile or compressive stress, significant effects such as the band alignment shift from type I to type III, and the transition from indirect to direct bandgap occurred. In conclusion, these research findings provide theoretical guidance for designing new heterojunctions based on SnBr2 . [ABSTRACT FROM AUTHOR]- Published
- 2023
- Full Text
- View/download PDF
42. Interface Coordination Engineering of P-Fe 3 O 4 /Fe@C Derived from an Iron-Based Metal Organic Framework for pH-Universal Water Splitting.
- Author
-
Fan, Minmin, Li, Peixiao, Liu, Baibai, Gong, Yun, Luo, Chengling, Yang, Kun, Liu, Xinjuan, Fan, Jinchen, and Xue, Yuhua
- Subjects
HYDROGEN evolution reactions ,METAL-organic frameworks ,ENERGY conversion ,ENGINEERING ,ENERGY consumption ,ELECTROCATALYSTS - Abstract
Developing electrocatalysts with high energy conversion efficiency is urgently needed. In this work, P-Fe
3 O4 /Fe@C electrodes with rich under-coordinated Fe atom interfaces are constructed for efficient pH-universal water splitting. The introduction of under-coordinated Fe atoms into the P-Fe3 O4 /Fe@C interface can increase the local charge density and polarize the 3d orbital lone electrons, which promotes water adsorption and activation to release more H* , thus elevating electrocatalytic activity. As a donor-like catalyst, P-Fe3 O4 /Fe@C displays excellent electrocatalytic performance with overpotentials of 160 mV and 214 mV in acidic and alkaline electrolytes at 10 mA cm−2 , in addition to pH-universal long-term stability. [ABSTRACT FROM AUTHOR]- Published
- 2023
- Full Text
- View/download PDF
43. Yam Bean (Pachyrhizus erosus L. Urban) Powder Improves Grass Carp Myofibrillar Protein Gel by Forming Disulfide Bonds, Hydrogen Bonds, and Proper Microstructure.
- Author
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Song, Geyao, Yang, Kun, Wu, Di, Lu, Weiwei, Chi, Rongshuo, Ma, Jing, and Sun, Weiqing
- Subjects
CTENOPHARYNGODON idella ,HYDROGEN bonding ,YAMS ,BEANS ,POWDERS ,POLYMERIZATION ,GELATION - Abstract
This study aimed to analyze the impact of different additions (0–1.25%) of yam bean powder (YBP) on myofibrillar protein (MP) gel characteristics such as the structure, water-holding capacity (WHC), chemical interaction strength of grass carp MP, and texture. The results showed that the YBP exhibited a strong water absorption capacity and filled in the protein heat-induced polymerization gel network well, which enabled the gel network to capture and retain water effectively, resulting in MP gels containing YBP with excellent WHC and gel strength (0.75%). In addition, YBP induced the formation of hydrogen and disulfide bonds in proteins and inhibited the conversion of α-helices to β-sheets and β-turn structures, facilitating the formation of high-strength gel networks (p < 0.05). In conclusion, YBP can significantly improve the thermally induced gelling properties of grass carp MP. In particular, the addition of 0.75% YBP had the best effect in terms of filling the gel network of grass carp MP, resulting in the formation of a continuous and dense protein network, leading to the composite gel with the best WHC and texture. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
44. Uncertainty Quantification of Satellite Soil Moisture Retrieved Precipitation in the Central Tibetan Plateau.
- Author
-
Zhang, Ke, Zhao, Long, Yang, Kun, Song, Lisheng, Ni, Xiang, Han, Xujun, Ma, Mingguo, and Fan, Lei
- Subjects
SOIL moisture ,STANDARD deviations ,PRECIPITATION (Chemistry) ,RAIN gauges - Abstract
SM2RAIN is a well-established methodology for estimating precipitation from satellite or observed soil moisture and it has been applied as a complementary approach to conventional precipitation monitoring methods. However, satellite soil moisture retrievals are usually subject to various biases and limited number of retrievals (and therefore large intervals) in remote areas, such as the Tibetan Plateau (TP), and little is known about their potential impacts on precipitation estimation. This study seeks to quantify the uncertainties in Soil Moisture Active and Passive (SMAP) soil moisture estimated precipitation through the commonly used SM2RAIN by referring to in situ soil moisture observations from the central Tibetan Plateau soil moisture network. The estimated precipitation is evaluated against rain gauge observations. Additional attention is paid to different orbits of the SMAP retrievals. Results show that the original SM2RAIN algorithm tends to underestimate the precipitation amount in the central TP when using SMAP soil moisture retrievals as input. The retrieval accuracy and sampling interval of SMAP soil moisture from ascending (descending) orbits each count for 1.04 mm/5 d (−0.18 mm/5 d) and 1.67 mm/5 d (0.72 mm/5 d) of estimated precipitation uncertainties as represented by root mean square error. Besides, the descending product of SMAP with a relatively less sampling interval and higher retrieval accuracy outperforms the ascending one in estimating precipitation, and the combination of both two orbits does add value to the overall SM2RAIN estimation. This study is expected to provide guidance for future applications of SM2RAIN-derived precipitation. Meanwhile, more reliable SM2RAIN precipitation estimations are desired when using higher quality satellite soil moisture products with better retrieval accuracy and smaller intervals. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
45. Fine Resolution Classification of New Ice, Young Ice, and First-Year Ice Based on Feature Selection from Gaofen-3 Quad-Polarization SAR.
- Author
-
Yang, Kun, Li, Haiyan, Perrie, William, Scharien, Randall Kenneth, Wu, Jin, Zhang, Menghao, and Xu, Fan
- Subjects
- *
SEA ice , *FEATURE selection , *ICE , *UNCERTAINTY (Information theory) , *SYNTHETIC aperture radar , *RANDOM forest algorithms , *MELTING points - Abstract
A new method of sea ice classification based on feature selection from Gaofen-3 polarimetric Synthetic Aperture Radar (SAR) observations was proposed. The new approach classifies sea ice into four categories: open water (OW), new ice (NI), young ice (YI), and first-year ice (FYI). Seventy parameters that have previously been applied to sea ice studies were re-examined for sea ice classification in the Okhotsk Sea near the melting point on 28 February 2020. The 'separability index (SI)' was used for the selection of optimal features for sea ice classification. Full polarization parameters (the backscatter intensity contains the horizontal transmit-receive intensity ( σ h h 0 ), Shannon entropy (SEi), the spherical scattering component of Krogager decomposition (Ks)), and hybrid polarization parameters ( h o r i z o n t a l r e c e i v e i n t e n s i t y (σ r h 0) , hybrid-pol Shannon entropy (CPSEi), the correlation coefficient (ρ r h − r v) between the σ r h 0 and σ r v 0 , and t h e s u r f a c e s c a t t e r i n g c o m p o n e n t o f m − α d e c o m p o s i t i o n α s ) were determined as the optimal parameters for the different work modes of SAR. The selected parameters were used to classify sea ice by the random forest classifier (RFC), and classification results were validated by manually interpreted ice maps derived from Landsat-8 data. The classification accuracy of OW, NI, YI and FYI reached 95%, 96%, 98% and 85%, respectively. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
46. MXene-Functionalized Light-Induced Antimicrobial and Waterproof Polyacrylate Coating for Cementitious Materials Protection.
- Author
-
Zhang, Hongping, Tang, Pengfei, Tang, Youhong, Yang, Kun, and Wang, Qingyuan
- Subjects
PROTECTIVE coatings ,SURFACE coatings ,ESCHERICHIA coli ,DETERIORATION of concrete ,WATERPROOFING ,EROSION - Abstract
The penetration of external stimuli (microorganisms, ions, etc.) following to the pore is the key reason for the deterioration of cement and concrete structures. Although the traditional methods such as improving the chemical composition of cement and concrete materials can delay the erosion rate, the inevitable pore structure still makes its deterioration a challenge. Based on this, we reported a protective coating for cementitious materials based on phenol and Ti
3 C2 MXene-modified polyacrylate (MXene-PG/PA). The introduction of phenols enhanced the waterproof properties of polyacrylate by increasing the interaction among molecular chains. Moreover, the introduction of Ti3 C2 MXene also endows the MXene-PG/PA coating with good light-induced antimicrobial properties. Beneficial to these designs, the MXene-PG/PA coating exhibited good waterproof properties (the water absorption ratio in seawater decreased by 58.2%) and antimicrobial properties (inhibition of E. coli and S. epidermidis activity under light). These results not only confirm that the MXene-PG/PA coating is a potential candidate of protective coating for cement-based materials, but also provide a new strategy for the design of multifunctional protective coatings. [ABSTRACT FROM AUTHOR]- Published
- 2023
- Full Text
- View/download PDF
47. Detection and Classification of Cotton Foreign Fibers Based on Polarization Imaging and Improved YOLOv5.
- Author
-
Wang, Rui, Zhang, Zhi-Feng, Yang, Ben, Xi, Hai-Qi, Zhai, Yu-Sheng, Zhang, Rui-Liang, Geng, Li-Jie, Chen, Zhi-Yong, and Yang, Kun
- Subjects
COTTON fibers ,COTTON ,OBJECT recognition (Computer vision) ,YARN ,OPTICAL polarization ,FEATURE extraction ,CLASSIFICATION algorithms ,PLANT fibers - Abstract
It is important to detect and classify foreign fibers in cotton, especially white and transparent foreign fibers, to produce subsequent yarn and textile quality. There are some problems in the actual cotton foreign fiber removing process, such as some foreign fibers missing inspection, low recognition accuracy of small foreign fibers, and low detection speed. A polarization imaging device of cotton foreign fiber was constructed based on the difference in optical properties and polarization characteristics between cotton fibers. An object detection and classification algorithm based on an improved YOLOv5 was proposed to achieve small foreign fiber recognition and classification. The methods were as follows: (1) The lightweight network Shufflenetv2 with the Hard-Swish activation function was used as the backbone feature extraction network to improve the detection speed and reduce the model volume. (2) The PANet network connection of YOLOv5 was modified to obtain a fine-grained feature map to improve the detection accuracy for small targets. (3) A CA attention module was added to the YOLOv5 network to increase the weight of the useful features while suppressing the weight of invalid features to improve the detection accuracy of foreign fiber targets. Moreover, we conducted ablation experiments on the improved strategy. The model volume, mAP@0.5, mAP@0.5:0.95, and FPS of the improved YOLOv5 were up to 0.75 MB, 96.9%, 59.9%, and 385 f/s, respectively, compared to YOLOv5, and the improved YOLOv5 increased by 1.03%, 7.13%, and 126.47%, respectively, which proves that the method can be applied to the vision system of an actual production line for cotton foreign fiber detection. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
48. The States and Properties of Germanium in Sulfuric Acid Solution.
- Author
-
Song, Leiting, Di, Haokai, Liang, Ming, Hong, Yan, Zeng, Yiner, Yang, Kun, and Zhang, Libo
- Subjects
SULFURIC acid ,GERMANIUM ,ACID solutions ,COLLOIDS ,ZETA potential - Abstract
In recent years, with the development of science and technology, the strategic position of germanium is becoming more and more important, and the global demand for germanium is also increasing. At present, there is no unified description of the existence form of germanium in solutions. Based on the current mainstream acid leaching process of germanium, this paper studies the existing form and properties of germanium in sulfuric acid solutions. Through the characterization and analysis of Raman, FTIR, and XPS of three concentrations of pure germanium solution, it is clear that germanium mainly exists in the form of H
2 GeO3 and some Ge4+ in sulfuric acid solution. Through the Tyndall effect and zeta potential, it is determined that H2 GeO3 exists in the form of colloid in sulfuric acid solution. With the increase of germanium concentration, H2 GeO3 will polymerize in a sulfuric acid solution to form polygermanic acid, and the H2 GeO3 colloidal dispersion system becomes more stable. This study clarifies the existing form and properties of germanium in sulfuric acid solution, which is of great significance to the leaching extraction of germanium-containing materials and the development of the germanium industry. [ABSTRACT FROM AUTHOR]- Published
- 2023
- Full Text
- View/download PDF
49. Phase Field Study on the Spinodal Decomposition of β Phase in Zr–Nb-Ti Alloys.
- Author
-
Yang, Kun, Wang, Yanghe, Tang, Jingjing, Wang, Zixuan, Zhang, Dechuang, Dai, Yilong, and Lin, Jianguo
- Subjects
- *
AGING - Abstract
In this study, a phase field method based on the Cahn–Hilliard equation was used to simulate the spinodal decomposition in Zr-Nb-Ti alloys, and the effects of Ti concentration and aging temperature (800–925 K) on the spinodal structure of the alloys for 1000 min were investigated. It was found that the spinodal decomposition occurred in the Zr-40Nb-20Ti, Zr-40Nb-25Ti and Zr-33Nb-29Ti alloys aged at 900 K with the formation of the Ti-rich phases and Ti-poor phases. The spinodal phases in the Zr-40Nb-20Ti, Zr-40Nb-25Ti and Zr-33Nb-29Ti alloys aged at 900 K were in an interconnected non-oriented maze-like shape, a discrete droplet-like shape and a clustering sheet-like shape in the early aging period, respectively. With the increase in Ti concentration of the Zr-Nb-Ti alloys, the wavelength of the concentration modulation increased but amplitude decreased. The aging temperature had an important influence on the spinodal decomposition of the Zr-Nb-Ti alloy system. For the Zr-40Nb-25Ti alloy, with the increase in the aging temperature, the shape of the rich Zr phase changed from an interconnected non-oriented maze-like shape to a discrete droplet-like shape, and the wavelength of the concentration modulate increased quickly to a stable value, but the amplitude decreased in the alloy. As the aging temperature increased to 925 K, the spinodal decomposition did not occur in the Zr-40Nb-25Ti alloy. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
50. DoMYB5 and DobHLH24, Transcription Factors Involved in Regulating Anthocyanin Accumulation in Dendrobium officinale.
- Author
-
Yang, Kun, Hou, Yibin, Wu, Mei, Pan, Qiuyu, Xie, Yilong, Zhang, Yusen, Sun, Fenghang, Zhang, Zhizhong, and Wu, Jinghua
- Subjects
- *
ANTHOCYANINS , *DENDROBIUM , *TRANSCRIPTION factors , *MEDICINAL plants , *HETERODIMERS , *MOLECULAR cloning - Abstract
As a kind of orchid plant with both medicinal and ornamental value, Dendrobium officinale has garnered increasing research attention in recent years. The MYB and bHLH transcription factors play important roles in the synthesis and accumulation of anthocyanin. However, how MYB and bHLH transcription factors work in the synthesis and accumulation of anthocyanin in D. officinale is still unclear. In this study, we cloned and characterized one MYB and one bHLH transcription factor, namely, D. officinale MYB5 (DoMYB5) and D. officinaleb bHLH24 (DobHLH24), respectively. Their expression levels were positively correlated with the anthocyanin content in the flowers, stems, and leaves of D. officinale varieties with different colors. The transient expression of DoMYB5 and DobHLH24 in D. officinale leaf and their stable expression in tobacco significantly promoted the accumulation of anthocyanin. Both DoMYB5 and DobHLH24 could directly bind to the promoters of D. officinale CHS (DoCHS) and D. officinale DFR (DoDFR) and regulate DoCHS and DoDFR expression. The co-transformation of the two transcription factors significantly enhanced the expression levels of DoCHS and DoDFR. DoMYB5 and DobHLH24 may enhance the regulatory effect by forming heterodimers. Drawing on the results of our experiments, we propose that DobHLH24 may function as a regulatory partner by interacting directly with DoMYB5 to stimulate anthocyanin accumulation in D. officinale. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
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