1. A Chemical Investigation of the Leaves of Morus alba L.
- Author
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Chen, Xiao-yan, Zhang, Ting, Wang, Xin, Hamann, Mark T., Kang, Jie, Yu, De-quan, and Chen, Ruo-yun
- Subjects
WHITE mulberry ,ANTIOXIDANTS ,METABOLITES ,CRYSTAL structure ,CHEMICAL reactions - Abstract
The leaves of
Morus alba L. are an important herbal medicine in Asia. The systematic isolation of the metabolites of the leaves ofMorus alba L. was achieved using a combination of liquid chromatography techniques. The structures were elucidated by spectroscopic data analysis and the absolute configuration was determined based on electronic circular dichroism (ECD) spectroscopic data and hydrolysis experiments. Their biological activity was evaluated using different biological assays, such as the assessment of their capacity to inhibit the aldose reductase enzyme; the determination of their cytotoxic activity and the evaluation of their neuroprotective effects against the deprivation of serum or against the presence of nicouline. Chemical investigation of the leaves ofMorus alba L. resulted in four new structures1 –4 and a known molecule5 . Compounds2 and5 inhibited aldose reductase with IC50 values of 4.33 μM and 6.0 μM compared with the potent AR inhibitor epalrestat (IC50 1.88 × 10−3 μM). Pretreatment with compound3 decreased PC12 cell apoptosis subsequent serum deprivation condition and pretreatment with compound5 decreased nicouline-induced PC12 cell apoptosis as compared with control cells (p < 0.001). [ABSTRACT FROM AUTHOR]- Published
- 2018
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