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3 results on '"Zhen-Feng Liu"'

1. Optimizing the Extraction of Anti-tumor Polysaccharides from the Fruit of Capparis spionosa L. by Response Surface Methodology

Author

Fang Dong, Dong-Bin Ma, Ling-Wen Zhang, Yu-Bin Ji, Zhen-Feng Liu, Jing Miao, and Li-Na Jin

Subjects
Capparis, Male, Time Factors, polysaccharides of Capparis spionosa L. (CSPS), extraction optimization, response surface methodology, anti-tumor activity, Central asia, Pharmaceutical Science, Chemical Fractionation, Polysaccharide, Article, Analytical Chemistry, Mice, Polysaccharides, Cell Line, Tumor, Neoplasms, Drug Discovery, Animals, Response surface methodology, Physical and Theoretical Chemistry, Antitumor activity, chemistry.chemical_classification, Chromatography, biology, Chemistry, Organic Chemistry, Extraction (chemistry), Chemical fractionation, Temperature, Reproducibility of Results, Water, biology.organism_classification, Antineoplastic Agents, Phytogenic, Chemistry (miscellaneous), Yield (chemistry), Fruit, Molecular Medicine, Female, Drugs, Chinese Herbal
Abstract

Capparis spionosa L. is a traditional medicinal plant in China and central Asia. In this study, an experiment was designed to investigate the optimization of the extraction of anti-tumor polysaccharides from the fruit of Capparis spionosa L. (CSPS) by response surface methodology (RSM). Four independent variables (extraction temperature, extraction time, ratio of water to sample and extraction cycles) were explored. Meanwhile, the in vivo anti-tumor activity of CSPS was investigated. The results showed that the experimental data could be fitted to a second-order polynomial equation using multiple regression analysis. The optimum extraction conditions were as follows: extraction temperature 92 °C, extraction time 140 min, ratio of water to sample 26 mL/g, and three extraction cycle. Under these conditions, the yield of polysaccharides reached 13.01%, which was comparable to the predicted yield (12.94%, p > 0.05). This indicated that the model was adequate for the extraction process. Additionally, CSPS could prolong the survival time of H22 bearing mice in vivo. The anti-tumor activities of CSPS were dose-dependent.

Published
2012

2. Optimization of Synthesis, Characterization and Cytotoxic Activity of Seleno-Capparis spionosa L. Polysaccharide.

Author

Yu-Bin Ji, Fang Dong, Lang Lang, Ling-Wen Zhang, Jing Miao, Zhen-Feng Liu, Li-Na Jin, and Ying Hao

Subjects
CAPPARIS spinosa, POLYSACCHARIDE synthesis, RESPONSE surfaces (Statistics), TEMPERATURE effect, CELL-mediated cytotoxicity, CANCER cells, ANTINEOPLASTIC agents, CHEMICAL reactions, EXPERIMENTS
Abstract

In this study, an experiment was designed to optimize the synthesis of seleno-Capparis spionosa L. polysaccharide (Se-CSPS) by response surface methodology. Three independent variables (reaction time, reaction temperature and ratio of Na2SeO3 to CSPS) were tested. Furthermore, the thermal stability, particle size, shape and cytotoxic activity of Se-CSPS in vitro were investigated. The optimum reaction conditions were obtained shown as follows: reaction time 7.5 h, reaction temperature 71 °C, and ratio of Na2SeO3 to CSPS 0.9 g/g. Under these conditions, the Se content in Se-CSPS reached 5.547 mg/g, which was close to the predicted value (5.518 mg/g) by the model. The thermal stability, particle size and shape of Se-CSPS were significantly different from those of CSPS. Additionally, a MTT assay indicated that the Se-CSPS could inhibit the proliferation of human gastric cancer SGC-7901 cells in a dose-dependent manner. [ABSTRACT FROM AUTHOR]

Published
2012
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3. Optimizing the Extraction of Anti-tumor Polysaccharides from the Fruit of Capparis spionosa L. by Response Surface Methodology.

Author

Yu-Bin Ji, Fang Dong, Dong-Bin Ma, Jing Miao, Li-Na Jin, Zhen-Feng Liu, and Ling-Wen Zhang

Subjects
CAPPARIS spinosa, FRUIT, POLYSACCHARIDES, BIOPOLYMERS, EXTRACTION (Chemistry), MULTIVARIATE analysis
Abstract

Capparis spionosa L. is a traditional medicinal plant in China and central Asia. In this study, an experiment was designed to investigate the optimization of the extraction of anti-tumor polysaccharides from the fruit of Capparis spionosa L. (CSPS) by response surface methodology (RSM). Four independent variables (extraction temperature, extraction time, ratio of water to sample and extraction cycles) were explored. Meanwhile, the in vivo anti-tumor activity of CSPS was investigated. The results showed that the experimental data could be fitted to a second-order polynomial equation using multiple regression analysis. The optimum extraction conditions were as follows: extraction temperature 92 °C, extraction time 140 min, ratio of water to sample 26 mL/g, and three extraction cycle. Under these conditions, the yield of polysaccharides reached 13.01%, which was comparable to the predicted yield (12.94%, p > 0.05). This indicated that the model was adequate for the extraction process. Additionally, CSPS could prolong the survival time of H22 bearing mice in vivo. The anti-tumor activities of CSPS were dose-dependent. [ABSTRACT FROM AUTHOR]

Published
2012
Full Text
View/download PDF

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