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Featured Application: SD-OCT imaging depth enhancement with improved contrast. We designed and fabricated a telecentric f-theta imaging lens (TFL) to improve the imaging performance of spectral domain optical coherence tomography (SD-OCT). By tailoring the field curvature aberration of the TFL, the flattened focal surface was well matched to the detector plane. Simulation results showed that the spot in the focal plane fitted well within a single pixel and the modulation transfer function at high spatial frequencies showed higher values compared with those of an achromatic doublet imaging lens, which are commonly used in SD-OCT spectrometers. The spectrometer using the TFL had an axial resolution of 7.8 μm, which was similar to the theoretical value of 6.2 μm. The spectrometer was constructed so that the achromatic doublet lens was replaced by the TFL. As a result, the SD-OCT imaging depth was improved by 13% (1.85 mm) on a 10 dB basis in the roll-off curve and showed better sensitivity at the same depth. The SD-OCT images of a multi-layered tape and a human palm proved that the TFL was able to achieve deeper imaging depth and better contrast. This feature was seen very clearly in the depth profile of the image. SD-OCT imaging performance can be improved simply by changing the spectrometer's imaging lens. By optimizing the imaging lens, deeper SD-OCT imaging can be achieved with improved sensitivity. [ABSTRACT FROM AUTHOR]

Dual-channel spectral domain optical coherence tomography (SD-OCT) is one of the effective methods for improving imaging depth and imaging speed. In this paper, we design a dual-channel SD-OCT system based on a single spectrometer that can operate in two modes: (1) Increasing imaging speed and (2) expanding imaging depth. An optical path offset is preintroduced between the two channels to separate the two-channel data. However, this offset increases the requirement for the spectral resolution of the spectrometer in mode (1), so compressive sensing (CS) technology is used herein to overcome this problem. Consequently, in mode (1), when the spectral resolution of the spectrometer is the same as that used in the single-channel system, we use a dual-channel SD-OCT system combined with CS technology to double the imaging speed. In mode (2), when the spectral resolution of the spectrometer is only half of that used in a single-channel system, the imaging depth can be nearly doubled. We demonstrate the feasibility and effectiveness of the method proposed in this work by imaging a mirror, a fish fin, a fish eye, and an onion. [ABSTRACT FROM AUTHOR]

In vivo tissue transparency in the visible light spectrum is beneficial for many research applications that use optical methods, whether it involves in vivo optical imaging of cells or their activity, or optical intervention to affect cells or their activity deep inside tissues, such as brain tissue. The classical view is that a tissue is transparent if it neither absorbs nor scatters light, and thus absorption and scattering are the key elements to be controlled to reach the necessary transparency. This review focuses on the latest genetic and chemical approaches for the decoloration of tissue pigments to reduce visible light absorption and the methods to reduce scattering in live tissues. We also discuss the possible molecules involved in transparency. [ABSTRACT FROM AUTHOR]