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Background/Objectives: The phase angle (PhA), as measured using bioelectrical impedance analysis (BIA), indicates muscle mass and quality. However, its relationship with age-related muscle changes and motor function and effective BIA assessment/intervention sites remains unclear. Herein, we evaluated age-related changes in PhA, explored the relationship between PhA and muscle mass, and identified effective sites for BIA. Methods: We included 131 healthy community-dwelling adults divided into older (≥65 years) and control (≤65 years) groups. PhA and muscle mass were measured using BIA with a device determining electrical resistance by transmitting a weak alternating current <90 μA. Motor function was measured using ground reaction force index, gait function, and grip strength. The relationships between muscle mass, PhA, and motor function were analyzed. Results: All values, excluding upper limb muscle mass, were lower in the older group, as were the rates of change in muscle mass and PhA in the lower limbs vs. the upper limbs. Lower limb PhA showed negative and positive correlations with the Timed Up and Go test and standing function, respectively; it was significantly associated with all motor functions. Conclusions: Regardless of age, muscle mass and PhA measured by BIA were high and low in the lower limbs, respectively, and lower limb PhA was related to walking and standing function. The decrease in lower limb muscle mass and PhA may accelerate with age. Given global population aging, easy-to-assess lower limb PhA linked to the movements necessary for independent living may be an effective site for assessment and intervention in clinical practice. [ABSTRACT FROM AUTHOR]

Background: Sarcopenia, which is characterized by the progressive loss of skeletal muscle mass, strength, and functionality, adversely affects cancer outcomes. This study aims to evaluate the development and progression of sarcopenia in patients with gastrointestinal cancer undergoing chemotherapy and its impact on comprehensive geriatric assessment outcomes in older participants. Methods: This cross-sectional study included 351 gastrointestinal cancer patients from October 2018 to December 2019. Pre- and post-chemotherapy measurements were taken for 243 participants. Sarcopenia was assessed using EWGSOP-2 criteria, including muscle mass, strength, and performance evaluations. A comprehensive geriatric assessment was conducted for patients aged 65 years and older. Results: The median age of participants was 57.84 years, with 31.7% being female and 29.2% being aged 65 years or older. A significant increase in the prevalence of sarcopenia post-chemotherapy was observed. The factors significantly associated with sarcopenia included low hand grip strength (−0.264; p < 0.001) and slow gait speed (0.222; p = 0.007). The muscle mass and albumin levels of older patients declined significantly post-treatment. Conclusions: This study highlights a strong association between chemotherapy and sarcopenia in gastrointestinal cancer patients, emphasizing the need for early detection and tailored interventions. Comprehensive geriatric assessments can provide critical insights that improve patient outcomes during chemotherapy. [ABSTRACT FROM AUTHOR]

Background/Objectives: Sarcopenia is an important clinical feature of patients with chronic liver disease (CLD). However, special devices are required to determine skeletal muscle mass. We evaluated the usefulness of body surface area (BSA) for estimating muscle mass and diagnosing sarcopenia in patients with CLD. Methods: We retrospectively studied 1889 Japanese patients with CLD who underwent bioimpedance analysis (BIA) (training cohort, n = 983; validation cohort, n = 906). The optimal cutoff values for predicting low skeletal muscle mass index (SMI) were determined using ROC analysis. We also assessed 1229 patients whose BSA and grip strength (GS) data were obtained on the same day and evaluated the diagnostic performance of the determined cutoff values of BSA for the diagnosis of sarcopenia. Results: In the training cohort, a strong correlation was observed between the SMI and BSA (r = 0.883, p < 0.0001). The cutoff values of BSA for predicting low SMI were 1.68 m2 for men and 1.48 m2 for women. Regarding the presence of low SMI, 776 (78.9%) and 730 (80.5%) patients were correctly diagnosed in the training and validation cohorts, respectively. The sensitivity and specificity of the combination of BSA and GS for sarcopenia were 82.7% and 97.1%, respectively, and 1175 patients (95.6%) were correctly diagnosed. Conclusions: BSA was highly correlated with SMI, suggesting that BSA could facilitate noninvasive estimation of low skeletal muscle mass in patients with CLD. [ABSTRACT FROM AUTHOR]

Featured Application: This study demonstrates that compartmental bioimpedance analysis, through the fat-to-muscle ratio, provides a simple and non-invasive method for identifying individuals at risk of exercise deficit disorder related to motor competence and physical fitness, as well as subsequent health risks. Consequently, it could be used to individualize physical exercise programs or physical education classes focusing on MC mastery and PF improvements based on muscle coordination and strength. This study aimed (1) to explore the association between body composition [fat-to-muscle ratio (FMR), waist circumference (WC), and body mass index (BMI)] with motor competence (MC) and physical fitness (PFI) in 7-year-old children; (2) to ascertain whether FMR modifies the associations between MC and PF. A total of 164 children (7 yr) were included. Anthropometric (fat and muscle mass were calculated with TANITA), motor competence (CAMSA test) and physical fitness (1/2 mile run, handgrip strength, 10 × 5, standing long jump, and sit and reach tests) variables were collected. FMR, WC and BMI correlated negatively with MC (all between r ≤ −0.333 and −0.183 and p < 0.05) and PFI (all between r ≤ −0.410 and −0.246 and p < 0.05) in all children. However, the associations were stronger for FMR. In linear regressions analyses, only FMR was independently and negatively associated with MC and PFI, explaining 12.6% and 20.9% of its variance, respectively. Positive correlations between PFI and MC existed. Also, the association was not modified by FMR levels, since in both groups (above and below FMR median), the associations were maintained (p < 0.0001). However, the t-test showed that children with higher FMRs have lower levels of MC (p = 0.005) and PFI (p < 0.0001). FMR may be the best body composition parameter related to MC and PFI. Furthermore, the association between MC and PFI is not modulated by FMR but children with higher FMRs show lower levels of MC and PFI. [ABSTRACT FROM AUTHOR]

Featured Application: The establishment of sports and health interventions based on different anthropometric profiles, considering the differences between the sexes, and a demonstration that physical activity is beneficial for health. Kinanthropometry is the study of body dimensions and composition measurements, which are influenced by factors such as age and nutritional status, establishing a relationship between static measurements and dynamic performance. This study aimed to evaluate the kinanthropometric differences among 403 individuals (aged 18–42), categorized by biological sex and the recreational sport they practiced. The main objective of this study was to clarify whether or not there were statistically significant differences between these groups. All of the measurements and indices were obtained following the International Society for the Advancement of Kinanthropometry (ISAK) protocol. Significant differences were found in most variables among the different sports. In general, the men showed higher values in terms of weight, height, body circumference, body mass index (BMI), relative index of the lower limbs (RILLs), percentage of muscle mass (%M), and percentage of residual mass (%R). The women exhibited higher values in terms of skinfold thicknesses, Cormic index (CI), body density index (BDI), percentage of fat mass (%F), and percentage of bone mass (%B). These findings can guide individuals in selecting sports based on their morphotype, optimizing their physical performance in recreational activities and improving their health and quality of life. [ABSTRACT FROM AUTHOR]

Background/Objectives: Thyroid hormones (THs) are correlated with body composition regardless of the presence of thyroid dysfunctions, whereas little is known regarding THs' influence on muscular fitness components. This cross-sectional study investigated whether THs may affect muscular parameters in adults who are overweight and affected obesity. Methods: One hundred and five volunteers (65 females and 40 males, BMI: 33.5 (8.0) kg/m2) in an euthyroid state were enrolled. Body composition was evaluated using bioelectrical impedance analysis. Muscular parameters of interest were grip strength—evaluated using the handgrip test (HG) and muscular performance –evaluated using the 10-repetition chair-stand test (CST). Free-triiodothyronine (FT3), free-thyroxine (FT4) and their ratio (FT3/FT4) were evaluated to assess thyroid function. Results: In the female group, the Pearson linear regression analysis revealed a positive correlation between FT3 and HG (R = 0.261, p = 0.036) and a negative correlation between FT3/FT4 and CST (R= −0.266, p= 0.032). In the male group, a positive correlation between FT3 and skeletal muscle mass was found (R = 0.354, p = 0.025). No correlations were found between THs of interest and adiposity indexes (BMI, fat mass) in either group (p > 0.05). Conclusion: FT3 and FT3/FT4 ratio levels in euthyroidism may influence muscular parameters differently in females and males affected by an excess of fat mass. More research is needed to understand the mechanisms behind this correlation and whether THs could be a potential biomarker for muscle-related parameters. [ABSTRACT FROM AUTHOR]

Sufficient protein intake has been shown to be advantageous for developing muscle mass, muscle strength, muscle quality, as well as for improving recovery from strenuous exercise, all of which are essential for athletic performance. Aging athletes, so-called master athletes, would benefit from evidence-based recommendations; however, studies investigating the role of their protein intake on muscle quality or performance are diverse and rare. Consequently, existing recommendations for this subpopulation of aging athletes are non-existent or speculative in nature. The aim of this short scoping review is to summarize available reports, identify common outcomes, and suggest future research directions. Literature research was carried out in PubMedMedline, SPORTDiscuss, and ScienceDirect without any restrictions regarding year of publication, type of research or sports discipline. Only observational and interventional studies with data on protein intake linked with body composition or performance outcomes were included for further analyses. We identified 12 suitable reports on master athletes with very diverse age-ranges, a broad variety of sports, and very heterogeneous outcome parameters. Seven studies investigated endurance athletes, four studies investigated multi-sports athletes, and only one study reported on strength-trained athletes. Average protein intake ranged between 1.0 and 1.9 g/kg/d. Within the few available studies, evidence tends to point towards a benefit of higher protein intakes for muscle mass and function; however, the low number of studies, combined with heterogeneity in study design and methods, limits their generalizability. Future studies are needed to build the evidence base for clear dietary recommendations respecting the specific needs of aging athletes. [ABSTRACT FROM AUTHOR]

Background/Objectives: Carbohydrate and protein restriction are associated with sarcopenia and osteopenia, but the underlying mechanisms remain unclear. We aimed to determine whether mild protein restriction affects muscle and bone function in wild-type (WT) and homozygous carbohydrate response element binding protein (Chrebp) knockout (KO) mice. Methods: Eighteen-week-old male wild-type and homozygous carbohydrate response element binding protein (Chrebp) knockout (KO) mice were fed a control diet (20% protein) or a low-protein diet (15% protein) for 12 weeks. We estimated the muscle weight and limb grip strength as well as the bone mineral density, bone structure, and bone morphometry. Results: Chrebp deletion and a low-protein diet additively decreased body weight (WT control–KO low-protein: mean difference with 95% CI, 8.7 [6.3, 11.0], p < 0.0001) and epidydimal fat weight (1.0 [0.7, 1.2], p < 0.0001). Chrebp deletion and a low-protein diet additively decreased tibialis anterior muscle weight (0.03 [0.01, 0.05], p = 0.002) and limb grip strength (63.9 [37.4, 90.5], p < 0.0001) due to a decrease in insulin/insulin-like growth factor 1 mRNA and an increase in myostatin mRNA. In contrast, Chrebp deletion increased bone mineral density (BMD) (WT control–KO control: –6.1 [–1.0, –2.3], p = 0.0009), stiffness (–21.4 [–38.8, –4.1], p = 0.011), cancellous bone BV/TV (–6.517 [–10.99, –2.040], p = 0.003), and the number of trabeculae (–1.1 [–1.8, –0.5], p = 0.0008). However, in KO mice, protein restriction additively decreased BMD (KO control–KO low-protein: 8.1 [4.3, 11.9], p < 0.0001), bone stiffness (38.0 [21.3, 54.7], p < 0.0001), cancellous bone BV/TV (7.7 [3.3, 12.2], p = 0.006), and the number of trabeculae (1.2 [0.6, 1.9], p = 0.0004). The effects of mild protein restriction on bone formation parameters (osteoid volume (WT control–WT low-protein: –1.7 [–2.7, –0.7], p = 0.001) and the osteoid surface (–11.2 [–20.8, –1.5], p = 0.02) were observed only in wild-type (WT) mice. The levels of bone resorption markers, such as the number of osteoclasts on the surface, the number of osteoclasts, and surface erosion, did not differ between the groups. Conclusions: Both Chrebp deletion and protein restriction led to a decrease in muscle and bone function; therefore, an adequate intake of carbohydrates and proteins is important for maintaining muscle and bone mass and function. Further studies will be needed to elucidate the mechanisms by which ChREBP deletion and a low-protein diet cause osteosarcopenia. [ABSTRACT FROM AUTHOR]

Type 2 diabetes mellitus (T2DM) is a common metabolic disease that is frequently accompanied by multiple complications, including diabetic myopathy, a muscle disorder that is mainly manifested as decreased muscle function and reduced muscle mass. Diabetic myopathy is a relatively common complication among patients with diabetes that is mainly attributed to mitochondrial dysfunction. Therefore, we investigated the mechanisms underlying diabetic myopathy development, focusing on the role of microRNAs (miRs). Zebrafish were fed a high-sugar diet for 8 weeks and immersed in a glucose solution to establish a model of T2DM. Notably, the fish exhibited impaired blood glucose homeostasis, increased lipid accumulation in the skeletal muscles, and decreased insulin levels in the skeletal muscle. Additionally, we observed various symptoms of diabetic myopathy, including a decreased cross-sectional area of skeletal muscle fibers, increased skeletal muscle fibrosis, a significant decline in exercise capacity, and a significant decrease in mitochondrial respiratory function. Mechanistically, bioinformatic analysis combined with various molecular analyses showed that the miR-139-5p/NAMPT pathway was involved in long-term high-glucose-induced mitochondrial dysfunction in the skeletal muscle, leading to diabetic myopathy. Conclusively, this study provides a basis for the development of novel strategies for the prevention and treatment of diabetic myopathy. [ABSTRACT FROM AUTHOR]

N6-methyladenosine (m6A) modification is a key methylation modification involved in reproductive processes. Myostatin gene editing (MT) in cattle is known to enhance muscle mass and productivity. However, the changes in m6A modification in MT bull sperm remain poorly understood. In the MT and wild-type (WT) groups, we identified 25,542 and 22,253 m6A peaks, respectively, mainly concentrated in the coding sequence (CDS) and 3′ untranslated region (UTR) of genes. The MT group showed an increase in gene transcription, but there was no significant difference in the overall m6A peaks pattern. There was also no significant difference in m6A motif and chromosome distribution between MT and WT groups. Most genes had less m6A modification sites. A total of 1120 m6A peaks were significantly different, corresponding to 1053 differentially m6A-methylated genes (DMMGs). These DMMGs are mainly associated with G protein-coupled receptor signaling pathways and the overall composition of the cell membrane. Furthermore, an MCL clustering analysis of 111 differentially m6A-methylated and expressed genes identified seven key genes (RHOA, DAAM1, EXOC4, GNA12, PRICKLE1, SCN1A, and STXBP5L), with the cytoskeleton and migration-related gene, RHOA, being the most important gene located at the center of the gene network. However, the analysis of sperm morphology and motility indicated no significant changes in semen volume, sperm count, sperm viability, plasma membrane integrity, acrosome membrane integrity, or mitochondrial membrane integrity. This study provides a map of m6A methylation in spermatozoa from MT and WT bulls, identifies key differential m6A genes that are affected by the myostatin gene but do not affect sperm morphology and viability in MT bulls, and provides a theoretical basis for the breeding quality of MT bulls. [ABSTRACT FROM AUTHOR]

Deterioration in muscle mass, strength, and physical performance due to conditions such as sarcopenia can affect daily activities and quality of life in the elderly. Exercise and mesenchymal stem cells (MSCs) are potential therapies for sarcopenia. This study evaluates the combined effects of exercise and adipose-derived MSCs (ADMSCs) in aged rats with sarcopenia. Eighteen-month-old rats were randomly divided into four groups: control, exercise (Ex), ADMSCs injection (MSC), and ADMSCs injection with exercise (MSC + Ex). Gastrocnemius (GCM) muscle mass increased in the Ex, MSC, and MSC + Ex groups compared to the control group. Although the mean CSA did not differ significantly between the groups, the size distribution of myofibers shifted toward larger sizes in the Ex and MSC + Ex groups. The MSC + Ex group performed best in functional tests, including the rotarod and hot plate tests. The protein expression levels of tumor necrosis factor (TNF) and the p-AMP-activated protein kinase (AMPK)/AMPK ratio in the GCM muscle were the lowest in the MSC + Ex group. This study demonstrates that combining exercise and ADMSC interventions was the most effective treatment for aged sarcopenic rats, suggesting a potential synergistic approach for sarcopenia treatment. [ABSTRACT FROM AUTHOR]

Background/Objectives: Sarcopenia, characterized by reduced muscle mass and strength, is associated with osteoarthritis (OA), particularly in middle-aged women, and may worsen postoperatively. Resistance exercise (RE) can resolve sarcopenia; however, recovery is often suboptimal. Nitrate (NO3−) supplementation may enhance muscle recovery and complement RE. We investigated whether NO3− supplementation combined with RE improves thigh muscle mass and strength in middle-aged women during postoperative rehabilitation. Methods: We conducted a prospective randomized placebo-controlled double-blind study including 36 middle-aged women with sarcopenia and cartilage defects undergoing mesenchymal stem cell implantation. Participants were assigned to RE with NO3− supplementation (NG, n = 18) or with placebo (PG, n = 18) groups. Both groups underwent 12 weeks of supervised RE. The primary outcomes were thigh muscle cross-sectional area (CSA) and knee strength, whereas functional and clinical measures, including the Short Physical Performance Battery (SPPB), skeletal muscle index (SMI), International Knee Documentation Committee (IKDC), and Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) scores, were secondary outcomes. Results: Thigh muscle CSA decreased in the PG but was maintained in the NG. Knee extension strength improved significantly in the NG compared with that in the PG at 6 and 12 weeks. Knee flexion strength also improved rapidly in the NG, with a significant increase at 6 weeks. SPPB and IKDC scores improved significantly in the NG. However, similar improvements were observed for WOMAC scores in both groups. Conclusions: NO3− supplementation combined with RE effectively prevented muscle atrophy and enhanced muscle strength in our study participants, indicating potential for improving postoperative recovery. [ABSTRACT FROM AUTHOR]

Dementia, including Alzheimer's disease, is a neurodegenerative illness characterized by the progressive impairment of cognitive functions, posing a significant global health threat. Physical exercise is widely recognized for its preventive role, providing benefits for both the body composition and brain health. This study aimed to explore the relationship between physical exercise, the body composition, and the progression of dementia. The analysis used clinical and neuroradiology data from 42 patients enrolled in the Alzheimer's Disease Neuroimaging Initiative (ADNI). Our study mainly focused on crucial parameters such as the body mass index (BMI), skeletal muscle index (SMI), and MRI biomarkers, including the hippocampal volume and white matter integrity. We grouped the participants according to the similarities of their body compositions through clustering techniques. Then, atrophy-related changes in the brain structures were computed using the Kullback–Leibler divergence. Our findings suggest that a higher BMI and greater muscle mass may slow down brain atrophy, suggesting a protective effect on the brain. Based on these results, preserving muscle mass and metabolic health through resistance and aerobic exercise appears crucial in reducing the risk of dementia. Body composition interventions may slow neurodegenerative changes and promote brain health. This is an essential piece of information about prevention strategies, especially for individuals at risk of dementia who may benefit from following structured physical activity strategies. [ABSTRACT FROM AUTHOR]

Sarcopenia, an age-related decline in skeletal muscle mass, strength, and function, is increasingly recognized as a significant condition in the aging population, particularly among those with cardiovascular diseases (CVD). This review provides a comprehensive synthesis of the interplay between sarcopenia and cardiogeriatrics, emphasizing shared mechanisms such as chronic low-grade inflammation (inflammaging), hormonal dysregulation, oxidative stress, and physical inactivity. Despite advancements in diagnostic frameworks, such as the EWGSOP2 and AWGS definitions, variability in criteria and assessment methods continues to challenge standardization. Key diagnostic tools include dual-energy X-ray absorptiometry (DXA) and bioimpedance analysis (BIA) for muscle mass, alongside functional measures such as grip strength and gait speed. The review highlights the bidirectional relationship between sarcopenia and cardiovascular conditions such as heart failure, aortic stenosis, and atherosclerotic cardiovascular disease, which exacerbate each other through complex pathophysiological mechanisms. Emerging therapeutic strategies targeting the mTOR pathway, NAD+ metabolism, and senescence-related processes offer promise in mitigating sarcopenia's progression. Additionally, integrated interventions combining resistance training, nutritional optimization, and novel anti-aging therapies hold significant potential for improving outcomes. This paper underscores critical gaps in the evidence, including the need for longitudinal studies to establish causality and the validation of advanced therapeutic approaches in clinical settings. Future research should leverage multi-omics technologies and machine learning to identify biomarkers and personalize interventions. Addressing these challenges is essential to reducing sarcopenia's burden and enhancing the quality of life for elderly individuals with comorbid cardiovascular conditions. This synthesis aims to guide future research and promote effective, individualized management strategies. [ABSTRACT FROM AUTHOR]

Background: Exoskeletons are used in rehabilitation centers for people with spinal cord injuries (SCI) due to the potential benefits they offer for locomotor rehabilitation. The acceptability of exoskeletons is crucial to promote rehabilitation and to ensure a successful implementation of this technology. The objective was to explore the acceptability of overground wearable powered exoskeleton used in rehabilitation among people with SCI. Methods: Fourteen individuals with SCI (9 men, mean [SD] age 47 years [14.8], a majority with traumatic and thoracic lesion (T6–T12)) who had utilized an exoskeleton in Canada or in France during their rehabilitation participated in a semi-structured interview. A thematic analysis using the theoretical framework of acceptability was carried out. Results: Participants were motivated to use an exoskeleton during their rehabilitation. They reported several perceived benefits to its use, including better walking pattern, increased endurance, and greater muscle mass. They also experienced mild pain, notable concentration demands, and fatigue. Most participants reported that using exoskeletons in their rehabilitation process was appropriate and relevant to them. Conclusions: Exoskeletons are generally well accepted by participants in this study. Adjustments in their use, such as conducting training sessions in obstacle-free environment and technological improvements to address the device's restrictive characteristics, heaviness, and massiveness are however still needed. [ABSTRACT FROM AUTHOR]

Background/Objectives: Monoterpenes, a class of organic compounds with the molecular formula C10H16, have garnered significant attention for their potential medicinal benefits. Emerging evidence suggests they may positively influence skeletal muscle function. However, the impact of monoterpene exposure on muscle strength and mass in humans remains unclear. Methods: To explore this relationship, we analyzed data from 1202 adults (aged ≥ 18 years) who participated in the 2013–2014 National Health and Nutrition Examination Survey (NHANES), focusing on serum levels of three specific monoterpenes—α-pinene, β-pinene, and limonene—and their association with hand grip strength and lean muscle mass. Results: Our analysis revealed that, except for test 2 of hand 1, all grip strength measures showed a positive correlation with ln-limonene levels. The β coefficient for combined grip strength was 2.409 (S.E. = 0.891, p = 0.015). Positive associations were also found between serum limonene levels and lean muscle mass. The β coefficient for the Appendicular Skeletal Muscle Mass Index (ASMI) was 0.138 (S.E. = 0.041, p = 0.004). Furthermore, combined grip strength and ASMI significantly increased across limonene quintiles (p for trend = 0.005 and 0.006, respectively). However, none of the three monoterpene levels showed a significant association with clinically defined low muscle mass or low muscle strength. Conclusions: Our findings suggest a plausible association between exposure to limonene, hand grip strength, and lean muscle mass among adults in the United States. Further investigation is needed to fully understand the underlying mechanisms and medical significance of this association. [ABSTRACT FROM AUTHOR]

Maximal physical effort induces a disturbance in the body's energy homeostasis and causes oxidative stress. The aim of the study was to determine whether prooxidant–antioxidant balance disturbances and the secretion of adipokines regulating metabolism, induced by maximal intensity exercise, are dependent on body composition in young, healthy, non-obese individuals. We determined changes in the concentration of advanced protein oxidation products (AOPP), markers of oxidative damage to nucleic acids (DNA/RNA/ox), and lipid peroxidation (LPO); catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) activity, as well as concentrations of visfatin, leptin, resistin, adiponectin, asprosin, and irisin in the blood before and after maximal intensity exercise in men with above-average muscle mass (NFAT-HLBM), above-average fat mass (HFAT-NLBM), and with average body composition (NFAT-NLBM). We corrected the post-exercise results for the percentage change in plasma volume. In all groups after exercise, there was an increase in LPO and resistin. In HFAT-NLBM, additionally, an increase in CAT and a decrease in SOD activity were noted, and in NFAT-NLBM, an increase in visfatin concentration was observed. In our study, the effect was demonstrated of a maximal effort on six (LPO, CAT, SOD, visfatin, resistin, and asprosin) of the twelve parameters investigated, while the effect of body composition on all parameters investigated was insignificant. Maximal intensity aerobic exercise induces secretion of resistin and damages lipids regardless of the exercising subjects' body composition. Large fat tissue content predisposes to exercise-induced disorders in the activity of antioxidant enzymes. We have also shown that it is necessary to consider changes in blood plasma volume in the assessment of post-exercise biochemical marker levels. [ABSTRACT FROM AUTHOR]

Cultured meat (CM) is derived from the in vitro myogenesis of muscle satellite (stem) cells (MSCs) and offers a promising alternative protein source. However, the development of a cost-effective media formulation that promotes cell growth has yet to be achieved. In this study, laxogenin (LAX) and 5-alpha-hydroxy-laxogenin (5HLAX) were computationally screened against myostatin (MSTN), a negative regulator of muscle mass, because of their antioxidant properties and dual roles as MSTN inhibitors and enhancers of myogenesis regulatory factors. In silico analysis showed LXG and 5HLXG bound to MSTN with binding free energies of −7.90 and −8.50 kcal/mol, respectively. At a concentration of 10 nM, LAX and 5HLAX effectively inhibited the mRNA and protein expressions of MSTN, promoted myogenesis, and enhanced myotube formation and maturation. In addition, by acting as agonists of ROS downregulating factors, they exhibited antioxidative effects. This study shows that supplementation with LAX or 5HLAX at 10 nM in CM production improves texture, quality, and nutritional value. We believe this study fills a research gap on media development for myotube formation and maturation, which are important factors for large-scale in vitro CM production that improve product quality, nutritional value, and efficacy. [ABSTRACT FROM AUTHOR]

Background: Acute diverticulitis (AD), an inflammatory complication of diverticulosis, affects around 4% of individuals with diverticulosis, with increased incidence in older populations. This study aims to assess the impact of sarcopenia, the age-related loss of muscle mass, on the clinical decision-making and outcomes of patients with AD. Materials and Methods: A retrospective study was conducted on 237 patients admitted to the Emergency Department (ED) between January 2014 and February 2022. Patients diagnosed with AD Hinchey ≥ 2 via contrasted tomography (CT) were included. Sarcopenia was assessed using CT scans at the third lumbar vertebra (L3), with skeletal muscle area (SMA) normalized by height to calculate the skeletal muscle index (SMI). Patients were divided into two groups based on sarcopenia status and analysed for surgical outcomes, non-operative management (NOM) success, and complications. Results: The prevalence of sarcopenia was 46%. Sarcopenic patients were significantly older and had lower BMI and higher frailty scores. A higher proportion of sarcopenic patients underwent Hartmann, while non-sarcopenic patients more often had anastomosis. Sarcopenia did not significantly affect overall morbidity, mortality, or the failure rate of NOM. However, sarcopenic patients with a BMI > 25 had a higher likelihood of requiring redo surgeries. Conclusions: Sarcopenia plays a critical role in the surgical management of AD but does not predict worse clinical outcomes. The decision to perform surgery, particularly Hartmann's procedure, is influenced by sarcopenia, yet morbidity and mortality rates are comparable between sarcopenic and non-sarcopenic patients. These findings highlight the need for sarcopenia to be considered in preoperative assessments. [ABSTRACT FROM AUTHOR]

Background/Objectives: The diaphragm is important for respiration, but the effects of age-related muscle loss and sarcopenia on diaphragm function are unclear. We evaluated the associations of sarcopenia and skeletal muscle mass (SMM) with diaphragm function. Methods: This study was conducted at three Japanese hospitals from May 2023 to September 2024. The participants underwent bioelectrical impedance for SMM assessment, as well as pulmonary function tests. Diaphragm ultrasound was used to measure the thickness at functional residual capacity (FRC), thickening fraction (TF), and diaphragm excursion (DE) during deep breathing (DB), and their associations with sarcopenia and low skeletal muscle index (SMI) were analyzed. Results: Overall, 148 patients (mean age 78.1 years; sarcopenia, n = 35; non-sarcopenia, n = 103) were included. No statistically significant differences in thickness(FRC), TF and DE were observed between the sarcopenia group and the non-sarcopenia group. The low SMI group had significantly lower thickness (difference −0.22, 95% CI; −0.41, −0.29) and DE (difference −9.2, 95%CI; −14.0, −4.49) than the normal SMI group. Multivariable linear regression analyses adjusted for age, sex, and stature revealed no association between thickness (FRC) and sarcopenia (p = 0.98), but thickness (FRC) was negatively associated with low SMI (p = 0.034). DE during DB was negatively associated with sarcopenia (p = 0.024) and low SMI (p = 0.001). TF showed no associations. Conclusions: DE during DB was reduced in patients with sarcopenia and low SMI, and thickness (FRC) was reduced in those with low SMI without sarcopenia. [ABSTRACT FROM AUTHOR]

Background: Hemodialysis patients face a high mortality risk, requiring effective clinical assessments. In these patients, muscle wasting due to protein-energy wasting (PEW) leads to increased frailty, which is strongly associated with worse outcomes, including higher mortality. As muscle mass declines, so does functional capacity, making regular assessment of both muscle mass and function critical for prognostic evaluation. Handgrip strength (HGS) offers a quick and reliable measure of muscle strength and functional capacity. In this study, we focused on the impact of HGS on survival in hemodialysis patients, analyzing its relationship with muscle mass and BMI. Methods: This retrospective cohort study included 408 dialysis patients (221 males, 187 females) who underwent bioimpedance spectroscopy (BIS) and HGS assessments between March 2021 and August 2023. Data collected included BIS profiles, HGS, dialysis status, age, complete blood count, blood chemistry, mortality, and CONUT scores. Results: Cox proportional hazards regression analysis revealed that lean tissue index (LTI) (HR 3.30, 95% CI 1.75–6.19), body mass index (BMI) (HR 2.65, 95% CI 1.17–6.01), and handgrip strength (HGS) (HR 4.22, 95% CI 2.05–8.70) were significant predictors of survival in the overall dialysis patient cohort. Gender-specific analysis showed that in males, both LTI (HR 4.81, 95% CI 1.89–12.23) and HGS (HR 5.45, 95% CI 2.18–13.61) significantly predicted survival. In females, HGS (HR 6.01, 95% CI 2.42–14.94) was a significant predictor, while LTI was also significant (HR 3.22, 95% CI 1.24–8.40, p = 0.017). In the multivariate Cox proportional hazards analysis, which adjusted for age, diabetes mellitus (DM), hypertension (HTN), BMI, fat tissue index (FTI), LTI, serum albumin, C-reactive protein (CRP), and CONUT score, HGS remained a significant predictor of survival in female dialysis patients (HR 2.77, 95% CI 1.00–7.65, p = 0.049). Conclusions: HGS has been identified as an important factor for survival in dialysis patients, particularly in female patients, independent of muscle mass and BMI. [ABSTRACT FROM AUTHOR]

Sarcopenia is an age-associated condition characterized by a muscle mass and function decline. This condition poses significant health risks for the elderly. This study developed a machine-learning model to predict sarcopenia using data from 664 participants. Key features were identified using the Local Interpretable Model-Agnostic Explanations (LIME) method. This enhanced model interpretability. Additionally, the CatBoost algorithm was used for training, and SMOTE-Tomek addressed dataset imbalance. Notably, the reduced-feature model outperformed the full-feature model, achieving an accuracy of 0.89 and an AUC of 0.94. The results highlight the importance of feature selection for improving model efficiency and interpretability in clinical applications. This approach provides valuable insights into the early identification and management of sarcopenia, contributing to better patient outcomes. [ABSTRACT FROM AUTHOR]

This study developed an advanced ensemble learning model aimed to improve the accuracy of predicting sarcopenia, a condition characterized by a gradual decline in muscle mass and strength, leading to increased disability and mortality. The study focused on enhancing model performance by combining various machine learning methods and addressing critical challenges, such as class imbalance and data complexity. Several foundational models were employed, including support vector machine, random forest, neural network, logistic regression, and decision tree. To address class imbalance, the adaptive synthetic sampling method was implemented, producing synthetic samples for the minority class to achieve a more balanced dataset. The data preprocessing stage included feature scaling and feature selection processes, utilizing recursive feature elimination to refine feature selection. Subsequently, a classifier selection algorithm was employed to select models that provided an optimal balance of diversity and performance. The effectiveness of the final ensemble model was evaluated using various metrics, such as accuracy, precision, recall, F1-score, and ROC AUC. The model achieved an accuracy of 88.5%, outperforming individual machine learning models and existing methods in the literature. These findings suggest that the classifier selection algorithm effectively addresses challenges in sarcopenia prediction, particularly in the case of imbalanced data. The model's strong performance indicates its potential for use in clinical environments, where it can facilitate early diagnosis and improve intervention strategies for sarcopenia patients. This study advances the field of medical machine learning by demonstrating the utility of ensemble learning in healthcare prediction. [ABSTRACT FROM AUTHOR]

Background/Objectives: Sarcopenia, characterized by the progressive loss of muscle mass and strength, is linked to physical disability, metabolic dysfunction, and an increased risk of mortality. Exercise therapy is currently acknowledged as a viable approach for addressing sarcopenia. Nevertheless, the molecular mechanisms behind exercise training or physical activity remain poorly understood. The disruption of mitochondrial homeostasis is implicated in the pathogenesis of sarcopenia. Exercise training effectively delays the onset of sarcopenia by significantly maintaining mitochondrial homeostasis, including promoting mitophagy, improving mitochondrial biogenesis, balancing mitochondrial dynamics, and maintaining mitochondrial redox. Exerkines (e.g., adipokines, myokines, hepatokines, and osteokines), signaling molecules released in response to exercise training, may potentially contribute to skeletal muscle metabolism through ameliorating mitochondrial homeostasis, reducing inflammation, and regulating protein synthesis as a defense against sarcopenia. Methods: In this review, we provide a detailed summary of exercise-induced exerkines and confer their benefit, with particular focus on their impact on mitochondrial homeostasis in the context of sarcopenia. Results: Exercise induces substantial adaptations in skeletal muscle, including increased muscle mass, improved muscle regeneration and hypertrophy, elevated hormone release, and enhanced mitochondrial function. An expanding body of research highlights that exerkines have the potential to regulate processes such as mitophagy, mitochondrial biogenesis, dynamics, autophagy, and redox balance. These mechanisms contribute to the maintenance of mitochondrial homeostasis, thereby supporting skeletal muscle metabolism and mitochondrial health. Conclusions: Through a comprehensive investigation of the molecular mechanisms within mitochondria, the context reveals new insights into the potential of exerkines as key exercise-protective sensors for combating sarcopenia. [ABSTRACT FROM AUTHOR]

Nitrate is abundant in natural foods, especially plant-based foods, having the potential to enhance muscle function. However, its relationship with sarcopenia in the context of daily diet remains unexplored. This cohort study investigated the associations between dietary nitrate intake and sarcopenia, as well as related symptoms, using data including 28,229 participants with a mean follow-up of 9.37 years from the UK Biobank. Dietary nitrate intake was estimated using a comprehensive nitrate food database. Adjusted logistic regression models suggested potential inverse associations between total nitrate intake and risks of sarcopenia plus pre-sarcopenia (Sarc-Presarc), low hand grip strength (HGS), and low walking pace. Similar results were primarily observed for nitrate from plant-based foods. With higher intake, females appeared to have higher HGS and a decreased risk of Sarc-Presarc, while males exhibited a reduced risk of low walking pace. The inverse association between nitrate intake and low skeletal muscle mass index was more evident in individuals aged 65 and above. These associations seemed independent of antioxidants, though higher antioxidants might augment the protective effect against low walking pace. Mediation analyses indicated that protein homeostasis and blood pressure might mediate these associations. These findings suggested that a higher dietary nitrate intake from plant-based foods could contribute to sarcopenia prevention, though further research is needed to confirm these observations. [ABSTRACT FROM AUTHOR]

Skeletal muscle is associated with cardiometabolic health. The appendicular skeletal muscle index (aSMI) represents the skeletal muscle mass "corrected" for height and constitutes a clinically applicable feature of sarcopenia. The relation of the aSMI with dietary habits is not clear, especially in young adults. The present pilot study aimed to investigate the relationship between the aSMI and Mediterranean diet adherence in young adults. A cross-sectional study of 151 university students was conducted. Anthropometry and bioimpedance analysis (TANITA-MC780) were performed. The aSMI was calculated as the sum of the upper and lower extremity muscle masses divided by height squared. The HPAQ questionnaire was used for physical activity evaluation. A validated food frequency questionnaire was used for dietary assessment, and the Mediterranean Diet Score (MedDietScore) was calculated. Multivariate linear regression models with log-aSMI as a dependent variable were applied. The MedDietScore was positively related to log-aSMI in the total sample (B = 0.009, SE = 0.004, p = 0.05, R2 for total model = 71%) irrespective of gender, age, ΒΜΙ, smoking, physical activity, and academic year and in males (B = 0.027, SE = 0.011, p = 0.023 R2 for total model = 37%) irrespective οf age and ΒΜΙ. The positive association of the MedDietScore with aSMI supports the importance of the adoption of a Mediterranean dietary pattern—rich in plant proteins and antioxidant/anti-inflammatory compounds—in maintaining/attaining muscle mass in young subjects. [ABSTRACT FROM AUTHOR]

Sarcopenia corresponds to a decrease in muscle mass and strength. CCL5 is a new myokine whose expression, along with the CCR5 receptor, is increased in sarcopenic muscle. Therefore, we evaluated whether CCL5 and CCR5 induce a sarcopenic-like effect on skeletal muscle tissue and cultured muscle cells. Electroporation in the tibialis anterior (TA) muscle of mice was used to overexpress CCL5. The TA muscles were analyzed by measuring the fiber diameter, the content of sarcomeric proteins, and the gene expression of E3-ligases. C2C12 myotubes and single-isolated flexor digitorum brevis (FDB) fibers were also treated with recombinant CCL5 (rCCL5). The participation of CCR5 was evaluated using the antagonist maraviroc (MVC). Protein and structural analyses were performed. The results showed that TA overexpression of CCL5 led to sarcopenia by reducing muscle strength and mass, muscle-fiber diameter, and sarcomeric protein content, and by upregulating E3-ligases. The same sarcopenic phenotype was observed in myotubes and FDB fibers. We showed increased reactive oxygen species (ROS) production and carbonylated proteins, denoting oxidative stress induced by CCL5. When the CCR5 was antagonized, the effects produced by rCCL5 were prevented. In conclusion, we report for the first time that CCL5 is a novel myokine that exerts a sarcopenic-like effect through the CCR5 receptor. [ABSTRACT FROM AUTHOR]

This case study aimed to evaluate the body composition and several biochemical markers during a 7-month pre-competition training period of a natural male bodybuilder. The athlete monitored his nutrition, training variables, and daily physical activity during the preparation phase. At the beginning (W31), in the middle (W18), and one week before the contest (W1), measurements included body composition via DEXA, testosterone and cortisol hormonal concentrations, and lipid, blood, and liver biochemical markers via an automated hematology analyzer. A gradual decrease in energy intake (−27.6%) and increased daily activity (169.0%) was found. Fat mass decreased from 17.9 kg (W31) to 13.1 kg (W18) and 4.2 kg (W1), while lean body mass decreased from 69.9 kg (W31) to 68.2 kg (W18) and 66.7 kg (W1). Bone density decreased linearly, and bone mass decreased from W31 to W1 by 1.7%. Testosterone decreased from 5.4 ng·mL−1 (W31) to 5.3 ng·mL−1 (W18) and 4.4 ng·mL−1 (W1), while cortisol increased from 21.3 μg·dL−1 (W31) to 20.3 μg·dL−1 (W18) and 24.4 μg·dL−1 (W1). In conclusion, a slow rate of weight loss and training with repetitions nearly to failure, combined with weekly monitoring of training and nutrition, may significantly improve body composition. However, hormone concentration and bone mass will experience mild negative effects. [ABSTRACT FROM AUTHOR]

Multiple sclerosis (MS) is a neurodegenerative disease that often leads to severe disability. Although exercise, particularly strength training, improves health outcomes in MS, no standardized, reliable tool exists for functional assessment to inform tailored exercise prescriptions and patients' categorization. This study aimed to validate the reliability of the MScope battery, a comprehensive tool incorporating structural, functional, and neuromuscular assessments to objectively evaluate patients with MS. A reproducibility study was conducted with 40 MS patients who completed the MScope battery twice, 72 h apart. Tests included structural (muscle thickness of the vastus lateralis and rectus femoris), functional (sit-to-stand, timed up-and-go, 10 m and six-minute walking test), and neuromuscular tests (isometric strength during the squat and leg extension exercises and handgrip strength). Intraclass correlation coefficients (ICCs), Bland–Altman plots, and the coefficient of variation (CV) were used to evaluate intra- and inter-day reliability. The MScope battery showed high intra- and inter-day reliability, with ICC values ranging from 0.79 to 0.99. Muscle thickness measurements, along with handgrip strength, demonstrated excellent reliability (ICC > 0.9, CV < 5%). Functional tests, including the timed up-and-go, 10 m walk, and sit-to-stand variations, maintained consistent scores (ICC > 0.85, CV < 10%). The six-minute walking test exhibited excellent inter-day reliability (ICC = 0.94, CV < 4%). Neuromuscular assessments showed strong reliability with minor day-to-day variability (ICC = 0.75–0.95, CV < 8%). The battery's overall consistency supports its use as a reliable tool for assessing functional status in MS patients. [ABSTRACT FROM AUTHOR]

The global rise in obesity underscores the need for effective weight management strategies that address individual metabolic and hormonal variability, moving beyond the simplistic "calories in, calories out" model. Body types—ectomorph, mesomorph, and endomorph—provide a framework for understanding the differences in fat storage, muscle development, and energy expenditure, as each type responds uniquely to caloric intake and exercise. Variability in weight outcomes is influenced by factors such as genetic polymorphisms and epigenetic changes in hormonal signaling pathways and metabolic processes, as well as lifestyle factors, including nutrition, exercise, sleep, and stress. These factors impact the magnitude of lipogenesis and myofibrillar protein synthesis during overfeeding, as well as the extent of lipolysis and muscle proteolysis during caloric restriction, through complex mechanisms that involve changes in the resting metabolic rate, metabolic pathways, and hormonal profiles. Precision approaches, such as nutrigenomics, indirect calorimetry, and artificial-intelligence-based strategies, can potentially leverage these insights to create individualized weight management strategies aligned with each person's unique metabolic profile. By addressing these personalized factors, precision nutrition offers a promising pathway to sustainable and effective weight management outcomes. The main objective of this review is to examine the metabolic and hormonal adaptations driving variability in weight management outcomes and explore how precision nutrition can address these challenges through individualized strategies. [ABSTRACT FROM AUTHOR]

Previous studies have revealed that muscle atrophy emerges as a significant challenge faced by astronauts during prolonged missions in space. A loss in muscle mass results in a weakening of skeletal muscle strength and function, which will not only contribute to a decline in overall physical performance but also elevate the risk of various age-related diseases. Skeletal muscle atrophy in the microgravity environment is thought to be associated with changes in energy metabolism, protein metabolism, calcium ion homeostasis, myostatin levels, and apoptosis. Modulating some pathways could be a promising approach to mitigating muscle atrophy in the microgravity environment. This review serves as a comprehensive summary of research on the impact of microgravity on skeletal muscle, with the aim of providing insights into its pathogenesis and the development of effective treatments. [ABSTRACT FROM AUTHOR]

Background: Skeletal muscle mass depletion adversely affects critically ill patient outcomes. Standardized methods for assessing muscle mass in this population are limited, particularly regarding changes during ICU stays and their implications for risk stratification. Methods: In this secondary analysis of our prospective data registry of surgical ICU patients, we used a single slice extracted from a computed tomography scan to determine the patient's direction of absolute change in skeletal muscle mass between two different time points (−14 d to +0 d and +5 d to +21 d) during his or her critical illness. Results: In total, 98 surgical patients were included in the final analysis. A decrease in a patient's skeletal muscle mass is associated with prolonged mechanical ventilation compared to patients whose skeletal muscle mass remained the same or increased (415 vs. 42 h, p = 0.003). Patients losing skeletal muscle mass also needed to be ventilated more frequently (88.3% vs. 60.5%, p = 0.002), had a higher rate of tracheotomy (50.0% vs. 23.7%, p = 0.011), and had an increased ICU length of stay (22 vs. 13 days, p = 0.045). Conclusions: A decreased skeletal muscle index in early critical illness negatively impacts ventilation parameters, highlighting the importance of monitoring and managing muscle mass changes to optimize outcomes in ICU patients. [ABSTRACT FROM AUTHOR]

Background and Clinical Significance: Sarcopenia, characterized by muscle loss and fat infiltration, poses a significant health burden for aging populations. Quantitative Color 2D and 3D radiodensitometry provides a powerful tool to monitor muscle quality and quantity through CT imaging. This study assessed the impact of a ten-year-long home-bed gym exercise intervention on muscle quality in an elderly subject using CT-derived radiodensitometric analysis. The study involved two comparative analyses: Study A, which compared knee-to-ankle CT scans of the subject between 2013 and 2023; and Study B, which compared the subject's 2023 thigh CT scan with a cohort of 2500 elderly Icelandic individuals from the AGES-Reykjavik study. Case Presentation: A 70-year-old male began a home-based Full-Body In-Bed Gym exercise program in 2013. Quantitative muscle volume and radiodensity measurements were performed using CT at baseline and after ten years. Results: Study A shows significant improvements in muscle volume observed in the knee-to-ankle region, while a slower decline in radiodensity was noted, indicating substantial preservation of muscle quality despite the expected decay of ten-year aging. For instance, muscle volume increased by 15% in the left Soleus muscle and by 6% in the right Soleus muscle, while the average radiodensity decreased by 12–17 HU. The subject's thigh muscle quality at 80-years-old is above the AGES-Reykjavik's cohort average, with reduced fat infiltration. Conclusions: Long-term home Full-Body In-Bed Gym, a low-impact exercise, can mitigate aging sarcopenia, as evidenced by improved tissue radiodensity and muscle mass substantial preservation. This suggests potential applications in personalized healthcare strategies to enhance muscle preservation among aging populations. [ABSTRACT FROM AUTHOR]

Background: Muscle quality and mass in cancer patients have prognostic and diagnostic importance. Objectives: The objectives are to analyze agreement between gold-standard and bedside techniques for morphofunctional assessment. Methods: This cross-sectional study included 156 consecutive colorectal cancer outpatients that underwent computed tomography (CT) scanning at lumbar level 3 (L3), whole-body bioelectrical impedance analysis (BIA), point-of-care nutritional ultrasound® (US), anthropometry, and handgrip strength in the same day. Measured muscle biomarkers were stratified by sex, age, BMI-defined obesity, and malnutrition using Global Leadership in Malnutrition (GLIM) criteria. Whole-body estimations for muscle mass (MM) and fat-free mass were calculated using two different equations in CT (i.e., Shen, and Mourtzakis) and four different equations for BIA (i.e., Janssen, Talluri, Kanellakis, and Kotler). Muscle cross-sectional area at L3 was estimated using the USVALID equation in US. Different cut-off points for muscle atrophy and myosteatosis were applied. Sarcopenia was defined as muscle atrophy plus dynapenia. Intra-technique and inter-technique agreement were analyzed with Pearson, Lin (ρ), and Cohen (k) coefficients, Bland–Altman analyses, and hypothesis tests for measures of central tendency. Results: Intra-technique agreements on muscular atrophy (CT k = 0.134, BIA k = −0.037, US k = 0.127) and myosteatosis (CT k = 0.122) were low, but intra-technique agreement on sarcopenia in CT was fair (k = 0.394). Inter-technique agreement on muscular atrophy and sarcopenia were low. Neither CT and BIA (ρ = 0.468 to 0.772 depending on equation), nor CT and US (ρ = 0.642), were interchangeable. Amongst the BIA equations, MM by Janssen proved the best, with a 1.5 (3.6) kg bias, (−5.6, 8.6) kg LoA, and 9/156 (5.7%) measurements outside the LoA. Muscle biomarkers in all techniques were worse in aged, female, or malnourished participants. Obesity was associated with higher muscle mass or surface biomarkers in all techniques. Conclusions: Bedside techniques adequately detected patterns in skeletal muscle biomarkers, but lacked agreement with a reference technique in the study sample using the current methodology. [ABSTRACT FROM AUTHOR]

Background: Patients with rectal cancer (RC) are at risk of developing cancer-related cachexia, a complex metabolic syndrome that can negatively impact quality of life (QoL), treatment tolerance, and clinical response. Objectives: The aim of the study was to explore the possible associations of the novel European Organization for Research and Treatment of Cancer QoL Questionnaire—Cancer Cachexia (EORTC QLQ-CAX24) scores with body composition parameters and physical performance in patients with locally advanced RC (LARC). Methods: This prospective observational study involved RC patients evaluated at the dedicated outpatient clinic of Clinical Nutrition at the Fondazione Policlinico Agostino Gemelli IRCCS. Patients with a confirmed diagnosis of LARC were enrolled between January and December 2023. The body composition parameters were measured using the preoperative computed tomography scan at the level of the third lumbar vertebra as well as using bioimpedance analysis before and after the radiotherapy treatment. QoL was measured by the EORTC QLQ-C30 and EORTC QLQ-CAX24 questionnaires. Results: A total of 56 RC patients were enrolled. Significant associations (p < 0.05) were found between EORTC QLQ-CAX24 values and the presence of cachexia, body composition, handgrip strength, and malnutrition diagnosis. Muscle mass was significatively also associated with EORTC QLQ-CAX24 results, suggesting a link between subjective perception of QoL and objectively measured body composition. Conclusions: The EORTC CAX24 questionnaire can be an effective tool for monitoring changes in cachexia status during radiotherapy, enabling early detection of cachexia-related complications and timely intervention. [ABSTRACT FROM AUTHOR]

Background: Obesity is a common problem in adults with intellectual disability, but to our knowledge, there are no reports which address the problem of "sarcopenic obesity" in younger adults with intellectual disability. Methods: We performed an analysis of the body composition of 93 men and women with intellectual disability, and 70 matched men and women without intellectual disability, aged between 20 and 50 years. Results: Truncal Fat Index (TFI) was significantly higher in these younger adults with intellectual disability when compared to same-age individuals without intellectual disability, for both men and women. Additionally, using Skeletal Muscle Index (SMI), we found significant negative effects of truncal fat on skeletal muscle mass. These effects were much greater for those with intellectual disability, and especially for women with intellectual disability. Conclusions: This report underscores the importance of evaluation of skeletal muscle mass in all individuals with obesity, but this need appears to be greater for those with intellectual disability, especially women. [ABSTRACT FROM AUTHOR]

Background: The objective of this study was to examine the impacts of absolute cuff pressure blood flow restriction (A-BFR) training and incremental cuff pressure blood flow restriction (I-BFR) training, under equal cuff pressures, on body composition and maximal strength among untrained adults. Additionally, we aimed to compare these effects with those observed in high-load resistance training (HL-RT). Methods: Thirty-three adults without prior professional sports or resistance training experience were recruited and randomly assigned to three groups (n = 11 per group) for an 8-week training program, held three times weekly. The A-BFR group trained with a 20% 1RM load and a cuff occlusion pressure set at 190 mmHg. The I-BFR group initiated training with an occlusion pressure of 160 mmHg, which incrementally increased by 20 mmHg every two weeks, with other conditions mirroring those of the A-BFR group. The HL-RT group trained with a 70% 1RM load. Results: All three groups demonstrated a statistically significant improvement in lower-body maximal strength (p < 0.01), with no significant differences observed among the groups (p > 0.05). A notable increase in left-leg muscle mass was seen across all groups (p < 0.05). However, total muscle mass, right-leg muscle mass, fat-free mass, BMI, bone mineral density, and bone mineral content remained relatively unchanged (p > 0.05), with no significant differences among the groups (p > 0.05). Only the HL-RT group exhibited a significant increase in left-leg thigh circumference (p < 0.05), while right-leg thigh circumference remained stable (p > 0.05), with no significant intergroup differences (p > 0.05). Conclusions: While A-BFR and I-BFR did not yield statistically significant differences in overall training outcomes, A-BFR demonstrated a slightly stronger potential. A-BFR and I-BFR achieved comparable gains in muscle strength and improvements in body composition to those seen with HL-RT. However, HL-RT demonstrated more significant improvements in leg circumference. [ABSTRACT FROM AUTHOR]

The addition of ketoanalogues (KAs) to a low-protein diet has been shown to mitigate the progression of pre-dialysis chronic kidney disease (CKD). The addition of inulin and calcium citrate may add further benefits, given their nephroprotective effects. In this study, we tested the changes in estimated glomerular filtration rate (eGFR), CKD symptoms, body composition, and biochemical parameters after 6 months of diet supplementation with Cetolán III, a combination of KA, inulin, and calcium citrate. We included 76 adult patients diagnosed with CKD stages 3 and 4 and not treated with renal replacement therapy in a retrospective cohort. In this cohort, participants were followed through two clinic visits at 3 and 6 months after diet supplementation. We found a slight increase in eGFR at 3 and 6 months compared with baseline, as well as a decrease in the severity of CDK-related symptoms, fat mass, and muscle mass. We observed only a slight decrease in creatinine and uric acid after 6 months of follow-up. We did not find a remarkable change in anthropometric parameters (e.g., body mass index, waist circumference, and arm muscle area). This observational study suggests that addition of KA, inulin, and calcium citrate to a low protein- diet could be associated with an improvement in eGFR and symptom severity in CKD pre-dialysis. [ABSTRACT FROM AUTHOR]

Sarcopenia is characterized by a degeneration of muscle mass and strength that incurs impaired mobility, posing grievous impacts on the quality of life and well-being of older adults worldwide. In 2018, a new international consensus was formulated to incorporate ultrasound imaging of the rectus femoris (RF) muscle for early sarcopenia assessment. Nonetheless, current clinical RF muscle identification and delineation procedures are manual, subjective, inaccurate, and challenging. Thus, developing an effective AI-empowered RF segmentation model to streamline downstream sarcopenia assessment is highly desirable. Yet, this area of research readily goes unnoticed compared to other disciplines, and relevant research is desperately wanted, especially in comparison among traditional, classic, and cutting-edge segmentation networks. This study evaluated an emerging Automatic Segment Anything Model (AutoSAM) compared to the U-Net and nnU-Net models for RF segmentation on ultrasound images. We prospectively analyzed ultrasound images of 257 older adults (aged > 65) in a community setting from Hong Kong's District Elderly Community Centers. Three models were developed on a training set (n = 219) and independently evaluated on a testing set (n = 38) in aspects of DICE, Intersection-over-Union, Hausdorff Distance (HD), accuracy, precision, recall, as well as stability. The results indicated that the AutoSAM achieved the best segmentation agreement in all the evaluating metrics, consistently outperforming the U-Net and nnU-Net models. The results offered an effective state-of-the-art RF muscle segmentation tool for sarcopenia assessment in the future. [ABSTRACT FROM AUTHOR]

Background/Objectives: Sarcopenia is characterized by the loss of muscle mass and function, increases in mortality rate, and risk of comorbidities in the elderly. This study evaluated the effects of Alnus japonica hot water extract (AJHW) and its active compound, oregonin, on muscle atrophy and apoptosis in vitro. Methods: AJHW underwent phytochemical analysis. C2C12 cells were subjected to H2O2 and dexamethasone to induce oxidative stress and muscle loss, after which AJHW and oregonin were administered to assess their impacts on cell viability, apoptosis, muscle protein synthesis stimulation, and muscle protein degradation inhibition. Cell viability was assessed via an MTT assay, and apoptosis was analyzed by measuring Bcl-2, Bax, cleaved caspase-3, and cleaved PARP through Western blotting. Western blotting and RT-PCR were utilized to analyze MyoD, Myogenin, Atrogin-1, and MuRF1 protein and gene expression in a muscle atrophy model, as well as the Akt/mTOR and FoxO3α pathways. Results: AJHW was confirmed to contain oregonin, an active compound. AJHW and oregonin significantly increased cell viability and reduced apoptosis by upregulating Bcl-2 and downregulating Bax, cleaved caspase-3, and cleaved PARP. They significantly enhanced muscle protein synthesis through the upregulation of MyoD and Myogenin, while diminishing muscle degradation by downregulating Atrogin-1 and MuRF1. The activation of the Akt/mTOR pathway and inhibition of the FoxO3α pathway were also observed. Conclusions: AJHW and oregonin effectively prevented muscle cell apoptosis, promoted muscle protein synthesis, and inhibited muscle protein degradation in vitro. These results suggest that AJHW and oregonin could serve as therapeutic agents to prevent and treat sarcopenia. [ABSTRACT FROM AUTHOR]

The average life expectancy is increasing worldwide, but the same cannot be said for a healthy life expectancy (defined as the period during which a person can live a healthy and independent life). Therefore, a major challenge is how to extend healthy life expectancy. One option is to reduce age-related muscle atrophy (sarcopenia) and cognitive decline. Since there is no specific cure for frailty, the prevention of frailty based on nutrition and exercise is a new approach to achieve healthy longevity. Studies have shown that interventions combining nutritional supplements and exercise are effective in improving muscle strength, muscle mass, and walking speed. Thus, the search for drugs and functional foods to combat frailty has attracted researchers' attention. Whale meat extract (WME) contains many imidazole dipeptides, especially the unique component balenine, which has various functional anti-fatigue and antioxidant properties, and hypermobility effects. Here, we investigated the effects of WME on the aging and health of Caenorhabditis elegans (hereafter, C. elegans). mRNA expression analysis showed that WME prolongs the lifespan of C. elegans mainly through sir-2.1, daf-2, and daf-16, and the myo-3, unc-54, unc-22, and fhod-1 genes involved in locomotor function. The results of this study showed that the expression of the antioxidant enzymes sod-2 and sod-3 was also increased. This study may provide the basis for further research on WME as a food and supplement to slow aging and improve motor function. [ABSTRACT FROM AUTHOR]

Emanating from several decades of study into the effects of the aging process after spinal cord injury (SCI), "accelerated aging" has become a common expression as the SCI accelerates the onset of age-related pathologies. However, the aging process follows a distinct trajectory, characterized by unique patterns of decline that differ from those observed in the general population without SCI. Aging brings significant changes to muscles, bones, and hormones, impacting overall physical function. Muscle mass and strength begin to decrease with a reduction in muscle fibers and impaired repair mechanisms. Bones become susceptible to fractures as bone density decreases. Hormonal changes combined with decreased physical activity accelerate the reduction of muscle mass and increase in body fat. Muscle atrophy and skeletal muscle fiber type transformation occur rapidly and in a unique pattern after SCI. Bone loss develops more rapidly and results in an increased risk of fractures in body regions unique to individuals with SCI. Other factors, such as excessive adiposity, decreased testosterone and human growth hormone, and increased systemic inflammation, contribute to a higher risk of neuropathically driven obesity, dyslipidemia, glucose intolerance, insulin resistance, and increasing cardiovascular disease risk. Cardiorespiratory changes after SCI result in lower exercise heart rates, decreased oxygenation, and mitochondrial dysfunction. While it is important to acknowledge the accelerated aging processes after SCI, it is essential to recognize the distinct differences in the aging process between individuals without physical disabilities and those with SCI. These differences, influenced by neuropathology, indicate that it may be more accurate to describe the aging process in individuals with chronic SCI as neurogenic accelerated aging (NAA). Research should continue to address conditions associated with NAA and how to ameliorate the accelerated rate of premature age-related conditions. This review focuses on the NAA processes and the differences between them and the aging process in those without SCI. Recommendations are provided to help slow the development of premature aging conditions. [ABSTRACT FROM AUTHOR]

Background/Objectives: Artificial intelligence (AI)-based volumetric measurements for assessing sarcopenia are expected to offer comprehensive insight into three-dimensional muscle volume and distribution. Therefore, we investigated the role of sarcopenia using computed tomography (CT)-based automated AI volumetric muscle measurements in predicting neoadjuvant chemoradiotherapy (nCRT) response and prognosis in patients with rectal cancer who underwent nCRT. Methods: We retrospectively analyzed the data of patients who underwent nCRT followed by curative resection between March 2010 and August 2021. Sarcopenia was defined using the Q1 cutoff value of the volumetric skeletal muscle index (SMI). The association between pre-nCRT volumetric sarcopenia and nCRT response was analyzed using logistic regression. A Cox proportional hazards model was used to identify the prognostic value of the pre- and post-nCRT volumetric SMIs. Results: Notably, 22 (25.6%) of the 86 patients had volumetric sarcopenia. The sarcopenia group showed a poorer nCRT response than the non-sarcopenia group. Pre-nCRT sarcopenia was a significant predictor of poor nCRT response (OR, 0.34 [95% CI, 0.12–0.96]; p = 0.041). Furthermore, an increased volumetric SMI during nCRT was a more significant prognostic factor on recurrence-free survival (aHR, 0.26 [95% CI, 0.08–0.83]; p = 0.023) and overall survival (aHR, 0.41 [95% CI, 0.17–0.99]; p = 0.049) than a decreased SMI. Conclusions: Volumetric sarcopenia can be used to predict poor nCRT response. A reduction in volumetric sarcopenia can be a poor prognostic factor in patients with rectal cancer who undergo nCRT. [ABSTRACT FROM AUTHOR]

Background and aim: Metabolic dysfunction-associated steatotic liver disease (MASLD), previously known as non-alcoholic fatty liver disease (NAFLD) affects up to 25% of the population and causes significant morbidity and mortality. Sarcopenia, the loss of muscle strength and quantity/quality, shares multiple pathways with MASLD, pointing to their possible association. In this systematic review, we aimed to describe the association between low muscle mass and/or strength and/or performance and the presence, development, or severity of MASLD/NAFLD. Methods: A search was performed in PubMed, Web of Science, Scopus, and LILACS, on 16 October 2020, for relevant studies, using a comprehensive search query and following PRISMA guidelines for systematic review conduction and reporting. Results: The search yielded 1042 results, of which 42 full papers and 11 poster abstracts were included. NAFLD was determined by imaging (n = 27), non-invasive tests (n = 13), liver biopsy (n = 11), or transient elastography (n = 2), and its severity by liver biopsy (n = 10), non-invasive tests (n = 7), transient elastography (n = 7), or imaging (n = 4). Muscle mass was, in most cases, adjusted for weight (n = 25), body mass index (BMI; n = 13), or height (n = 10). Most studies showed an association of low muscle mass and low strength with NAFLD presence and severity. However, the association of muscle mass with NAFLD was present when muscle mass was adjusted for weight or BMI but not when it was adjusted for height. A meta-analysis was not possible due to high heterogeneity in the methods of assessment of muscle parameters and NAFLD. Conclusions: This systematic review suggests the need for standardization of methods in this discipline. The issuing of specific guidance regarding sarcopenia in NAFLD would be of importance. [ABSTRACT FROM AUTHOR]

Objectives: The aim of this study was (i) to design and develop a portable BCA device for measuring body composition parameters such as body weight, body fat (BF) %, total body water (TBW), fat-free mass (FFM), muscle mass (MM), and bone mass (BM); (ii) to validate the developed portable BCA with the Tanita MC 980 MA BCA device. Methods: For this current study, two hundred healthy and obese subjects, whose ages ranged from 8 to 12 years (8.4 ± 1.7), were considered. Results: The highest percentage difference between the two study groups was found to be in BFat (50.39%), followed by body mass index (BMI) (41.73 kg), FFM (38.32 kg), and MM (37.89 kg), and this was found to be statistically significant. The results obtained from the designed prototype of the body composition analyzer were validated using Tanita MC 980MA BCA. The overall error% was calculated as ±3% for measuring the different body composition parameters. Conclusions: Due to its low standard error and high overall accuracy, the BCA prototype demonstrates the potential to be a dependable instrument for evaluating and tracking the body composition of children. [ABSTRACT FROM AUTHOR]

Objectives: While the effectiveness of metabolic/bariatric surgery has been confirmed, understanding the factors associated with weight loss is paramount for providing guidance in postoperative treatment strategies. Here, we aimed to examine the factors associated with long-term maintenance of weight loss after laparoscopic sleeve gastrectomy (LSG). Methods: This prospective observational cohort included patients who underwent LSG at a single academic health center between January 2017 and June 2022. We examined their body composition using InBody 720 or 770 and analyzed the factors associated with the percentage of total weight loss (%TWL) for 24 months. Results: The median body mass index (BMI) was 38.8 (interquartile range [IQR]: 35.6–46.7) preoperatively, 32.7 kg/m2 (IQR: 28.2–38.7) at 12 months postoperatively, and 33.9 kg/m2 (IQR: 29.1–40.1) at 24 months postoperatively. The lowest BMI was observed at 12 months (p < 0.001 vs. preoperative), followed by a significant increase at 24 months (p = 0.003). However, BMI remained significantly lower at 24 months than preoperatively (p < 0.001). The skeletal muscle mass to fat mass ratio (SMM/FM) was 0.59 (IQR: 0.50–0.71) preoperatively, 0.79 (IQR: 0.58–1.26) at 12 months, and 0.70 (IQR: 0.54–1.05) at 24 months, peaking at 12 months (p < 0.001 vs. preoperative) and decreasing significantly by 24 months (p < 0.001). Nevertheless, the SMM/FM ratio at 24 months remained higher than preoperative values (p < 0.001). Median body weight and %TWL were 86.0 kg and 15.6%, respectively, at 24 months after LSG. The SMM/FM ratio at 12 months was positively correlated with %TWL at 24 months after adjusting for age and sex. Conclusions: The effects of LSG persisted for up to 24 months postoperatively. The SMM/FM ratio 12 months after LSG was associated with the rate of weight loss at 24 months. [ABSTRACT FROM AUTHOR]

Background and Objectives: Sodium glucose cotransporter-2 (SGLT-2) inhibitors have emerged as integral therapeutic tools in the management of patients with cardiovascular–kidney–metabolic (CKM) syndrome. In addition to their well-documented effects on lowering glucose levels and cardiovascular- and reno-protective actions, SGLT-2 inhibitors, through a reduction in body weight (BW), generate changes in the body composition and volume status that have not been clearly studied. Materials and Methods: This retrospective, observational longitudinal cohort, single-center study analyzed and compared body composition and fluid status measured by bioelectrical impedance analysis (BIA) from weeks 0 to 12 after the initiation of the cardiac rehabilitation (CR) program for coronary artery disease and heart failure in 59 patients who started treatment with SGLT-2 inhibitors (SGLT-2iG) and 112 patients without SGLT-2 inhibitors (non-SGLT-2iG). Results: Changes between the baseline and week 12 in the SGLT-2iG and non-SGLT-2iG were −0.3 L (p = 0.003) and −0.03 L (p = 0.82) in extracellular water (ECW) (p = 0.05), −0.39 L (p < 0.001) and −0.14 L (p = 0.33) in intracellular water (ICW) (p = 0.12), −0.69 (p < 0.001) and −0.16 (p = 0.52) in total body water (TBW) (p = 0.08), and −0.01 (p = 0.37) and −0.001 (p = 0.25) in the ECW/TBW ratio, respectively. After 3 months of exercise therapy in the CR program, patients in the SGLT-2iG showed a greater decrease than the non-SGLT-2iG in weight (−1.34 kg, p < 0.001 vs. −0.99, p = 0.02), body mass index (BMI) (−0.45 kg/m2, p < 0.001 vs. −0.38, p = 0.004), arm circumference (−0.57 cm, p = 0.008 vs. −0.12 cm, p = 0.21), waist circumference (−1.5 cm, p = 0.04 vs. −0.11 cm, p = 0.83), systolic blood pressure (SBP) (−8.9 mmHg, p = 0.049 vs. −4.19, p = 0.08), and diastolic blood pressure (DBP) (−5.15, p = 0.03 vs. −2.85, p = 0.01). The bioelectrical impedance analysis (BIA) revealed a significant decrease in body fat mass (BFM) and visceral fat area, without a loss of lean body mass (LBM) or skeletal muscle mass in the SGLT-2iG. Conclusions: SGLT-2 inhibitors exert beneficial effects on body compartments and volume status. Although they induce modest weight loss, this appears to be mainly directed at ECW, BFM, and visceral fat, without a loss of LBM nor skeletal muscle mass, which could contribute to the observed CKM benefits. [ABSTRACT FROM AUTHOR]

Simple Summary: Because of the treatments they have undergone, survivors of childhood brain tumors have a greater risk of cardiovascular disease and sarcopenia compared to the general population. The objective of this study was to analyze differences in a bioelectrical impedance analysis (BIA) of body composition between male childhood brain tumor cancer survivors and healthy controls and to evaluate the correlation between the BIA results and the treatments performed (chemotherapy, radiotherapy, and steroid therapy). Our analysis indicates the presence of several body composition indexes at the BIA that point towards the presence of sarcopenia in childhood brain tumor survivors, showing also a significant correlation between some of these indexes and total dose of carboplatin received. Background. Childhood brain tumor survivors (CCSs) are at high risk of developing metabolic syndrome (MetS) and sarcopenia. To date, a tool able to predict any body composition changes or detect them early and increased adiposity (and, therefore, increased likelihood of MetS onset) is still lacking in this population. Objective. The objective was to analyze differences in a bioelectrical impedance analysis (BIA) of body composition between male childhood brain tumor cancer survivors and healthy controls. Methods. In this pilot, prospective, observational study, 14 male CCSs were compared to 14 healthy controls matched for sex and age. Results. CCSs showed statistically significant lower mean values in terms of their body metabolic rate (BMR), body cell mass index (BCMI), fat-free mass (FFM), skeleton muscle mass (SM), skeletal muscle mass index (SMI), and appendicular skeletal muscular mass (ASMM). CCSs also showed a statistically significantly higher mean value of resistance when compared with controls. The BMR, BCM, FFM, and ASMM were significantly correlated with total doses of carboplatin (Tau = −0.601; p = 0.018; Tau = −0.599, p = 0.025; Tau = −0.601, p = 0.018; Tau = −0.509, p = 0.045, respectively). Conclusion. A BIA allows for the detection of changes in body composition in survivors of childhood brain tumors, revealing either the presence of central obesity correlated with the risk of MetS or signs of sarcopenia that deserve early treatment. [ABSTRACT FROM AUTHOR]

Age-dependent loss of muscle mass and function is associated with oxidative stress. DJ-1/Park7 acts as an antioxidant through multiple signalling pathways. DJ-1-knockout zebrafish show a decline in swimming performance and loss of weight gain between 6 and 9 months of age. Here, we address the degree to which this is associated with muscle degeneration and identify molecular changes preceding dysregulation of muscle performance. Loss of DJ-1 reduced the skeletal muscle fibre cross-section area. The highly mitochondrial-dependent red slow muscle was more affected than the white muscle, and degeneration of sub-sarcolemma red muscle mitochondria was observed. Using TandemMassTag-based quantitative proteomics, we identified a total of 3721 proteins in the multiplex sample of 4 and 12-month-old muscles. A total of 68 proteins, mainly associated with inflammation and mitochondrial function, were dysregulated in the young DJ-1-null adults, with Annexin A3, Sphingomyelin phosphodiesterase acid-like 3B, Complement C3a, and 2,4-dienoyl CoA reductase 1 being the most affected. The loss of DJ-1 also accelerated molecular features associated with sarcopenia, such as a decrease in the NAD+/NADH ratio and a reduction in Prostaglandin reductase 2 and Cytosolic glycerol-3-phosphate dehydrogenase levels. In view of the experimental power of zebrafish, the DJ-1-null zebrafish makes a valuable model for understanding the connection between oxidative stress and age-dependent muscle loss and function. [ABSTRACT FROM AUTHOR]

Background: Plant-based diets are associated with various health benefits; however, their impact on physical performance in aging populations remains unclear. Objectives: To investigate the associations between adherence to plant-based diets and physical performance, focusing on their potential protective effects against age-related declines in function. Methods: Data were obtained from men and women aged 40 years or older in the Baltimore Longitudinal Study of Aging (BLSA) (mean ± SD age: 68 ± 13 years at the first dietary visit; n = 1389). Dietary intake was assessed using a food frequency questionnaire (FFQ). Plant-based diets, calculated from 18 food groups, were categorized as overall (PDI), healthful (hPDI), or unhealthful (uPDI), and their tertiles across visits were analyzed. Multivariable linear mixed-effects models were used to examine the association between repeated measurements of three physical performance outcomes—Short Physical Performance Battery (SPPB), grip strength (kg), and gait speed (m/s)—and adherence to each plant-based diet. Results: In fully adjusted models, SPPB and grip strength were significantly associated with both hPDI and uPDI, but not with PDI. For hPDI, the intermediate tertile showed the greatest benefit, with SPPB scores 0.5 points higher (βT2vs.T1 = 0.50, 95% CI: 0.30–0.70, p < 0.001) over the follow-up period. In contrast, for uPDI, a 0.27-point lower SPPB score was seen (βT3vs.T1 = −0.27, 95% CI: −0.48 to −0.07, p = 0.009). Longitudinally, grip strength was positively associated with hPDI (βT3vs.T1 = 1.14, 95% CI: 0.24–2.05, p = 0.0013). Similar results were observed in older adults aged ≥65 years. Conclusions: Adherence to hPDI may benefit lower body function and muscle strength, while uPDI appears to have adverse effects. This suggests that the quality of plant-based foods is essential for maintaining functional well-being in older adults. Further research is needed to confirm these findings, explore underlying mechanisms, and identify strategies to optimize plant-based dietary patterns for aging populations. [ABSTRACT FROM AUTHOR]