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Your search keyword '"Avnet, Sofia"' showing total 19 results
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19 results on '"Avnet, Sofia"'

2. Behavioral Voluntary and Social Bioassays Enabling Identification of Complex and Sex-Dependent Pain-(-Related) Phenotypes in Rats with Bone Cancer

Author

Segelcke, Daniel, primary, Linnemann, Jan, additional, Pradier, Bruno, additional, Kronenberg, Daniel, additional, Stange, Richard, additional, Richter, S. Helene, additional, Görlich, Dennis, additional, Baldini, Nicola, additional, Di Pompo, Gemma, additional, Verri, Waldiceu A., additional, Avnet, Sofia, additional, and Pogatzki-Zahn, Esther M., additional

Published
2023
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8. The Release of Inflammatory Mediators from Acid-Stimulated Mesenchymal Stromal Cells Favours Tumour Invasiveness and Metastasis in Osteosarcoma

Author

Avnet, Sofia, primary, Lemma, Silvia, additional, Cortini, Margherita, additional, Di Pompo, Gemma, additional, Perut, Francesca, additional, Lipreri, Maria Veronica, additional, Roncuzzi, Laura, additional, Columbaro, Marta, additional, Errani, Costantino, additional, Longhi, Alessandra, additional, Zini, Nicoletta, additional, Heymann, Dominique, additional, Dominici, Massimo, additional, Grisendi, Giulia, additional, Golinelli, Giulia, additional, Consolino, Lorena, additional, Longo, Dario Livio, additional, Nanni, Cristina, additional, Righi, Alberto, additional, and Baldini, Nicola, additional

Published
2021
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12. Exploring Metabolic Adaptations to the Acidic Microenvironment of Osteosarcoma Cells Unveils Sphingosine 1-Phosphate as a Valuable Therapeutic Target

Author

Cortini, Margherita, primary, Armirotti, Andrea, additional, Columbaro, Marta, additional, Longo, Dario Livio, additional, Di Pompo, Gemma, additional, Cannas, Elena, additional, Maresca, Alessandra, additional, Errani, Costantino, additional, Longhi, Alessandra, additional, Righi, Alberto, additional, Carelli, Valerio, additional, Baldini, Nicola, additional, and Avnet, Sofia, additional

Published
2021
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17. The Effects of Systemic and Local Acidosis on Insulin Resistance and Signaling

Author

Nicola Baldini, Sofia Avnet, Baldini, Nicola, and Avnet, Sofia

Subjects
0301 basic medicine, medicine.medical_treatment, acid-sensing ion channel, Review, acidosi, lcsh:Chemistry, 0302 clinical medicine, Insulin, insulin receptor, lcsh:QH301-705.5, Spectroscopy, Acidosis, biology, Chemistry, General Medicine, acid-sensing ion channels, Computer Science Applications, 030220 oncology & carcinogenesis, acidosis, medicine.symptom, Signal Transduction, medicine.medical_specialty, Intracellular pH, glucose metabolism, Carbohydrate metabolism, Catalysis, Inorganic Chemistry, 03 medical and health sciences, Insulin resistance, ion/proton transporters, Internal medicine, Diabetes mellitus, medicine, Animals, Humans, Physical and Theoretical Chemistry, Molecular Biology, Ion Transport, Organic Chemistry, Glucose transporter, medicine.disease, Receptor, Insulin, Insulin receptor, 030104 developmental biology, Endocrinology, lcsh:Biology (General), lcsh:QD1-999, biology.protein, Insulin Resistance
Abstract

Most pathological conditions that cause local or systemic acidosis by overcoming the buffering activities of body fluids overlap with those diseases that are characterized by glucose metabolic disorders, including diabetes mellitus, inflammation, and cancer. This simple observation suggests the existence of a strong relationship between acidosis and insulin metabolism or insulin receptor signaling. In this review, we summarized the current knowledge on the activity of insulin on the induction of acidosis and, vice versa, on the effects of changes of extracellular and intracellular pH on insulin resistance. Insulin influences acidosis by promoting glycolysis. Although with an unclear mechanism, the lowering of pH, in turn, inhibits insulin sensitivity or activity. In addition to ketoacidosis that is frequently associated with diabetes, other important and more complex factors are involved in this delicate feedback mechanism. Among these, in this review we discussed the acid-mediated inhibiting effects on insulin binding affinity to its receptor, on glycolysis, on the recycling of glucose transporters, and on insulin secretion via transforming growth factor β (TGF-β) activity by pancreatic β-cells. Finally, we revised current data available on the mutual interaction between insulin signaling and the activity of ion/proton transporters and pH sensors, and on how acidosis may enhance insulin resistance through the Nuclear Factor kappa B (NF-κB) inflammatory pathway.

Published
2018

18. Validation of Suitable Housekeeping Genes for the Normalization of mRNA Expression for Studying Tumor Acidosis

Author

Michael Joseph Meade, Nicola Baldini, Sofia Avnet, Silvia Lemma, Tokuhiro Chano, Lemma, Silvia, Avnet, Sofia, Meade, Michael Joseph, Chano, Tokuhiro, and Baldini, Nicola

Subjects
0301 basic medicine, Reproducibility of Result, acidosi, Validation Studies as Topic, Biology, Article, Catalysis, lcsh:Chemistry, Inorganic Chemistry, 03 medical and health sciences, Neoplasms, Reference genes, Gene expression, medicine, Humans, tumor microenvironment, RNA, Messenger, Physical and Theoretical Chemistry, lcsh:QH301-705.5, Molecular Biology, Spectroscopy, housekeeping gene, Tumor microenvironment, Genes, Essential, Gene Expression Profiling, RT-qPCR, Organic Chemistry, Reproducibility of Results, Computational Biology, Cancer, General Medicine, Reference Standards, Hydrogen-Ion Concentration, medicine.disease, Computer Science Applications, Housekeeping gene, Gene Expression Regulation, Neoplastic, Gene expression profiling, 030104 developmental biology, lcsh:Biology (General), lcsh:QD1-999, YWHAZ, Cancer research, Neoplasm, Reference Standard, Osteosarcoma, acidosis, Human
Abstract

Similar to other types of cancer, acidification of tumor microenvironment is an important feature of osteosarcoma, and a major source of cellular stress that triggers cancer aggressiveness, drug resistance, and progression. Among the different effects of low extracellular pH on tumor cells, we have recently found that short-term exposure to acidosis strongly affects gene expression. This alteration might also occur for the most commonly used housekeeping genes (HKG), thereby causing erroneous interpretation of RT-qPCR data. On this basis, by using osteosarcoma cells cultured at different pH values, we aimed to identify the ideal HKG to be considered in studies on tumor-associated acidosis. We verified the stability of 15 commonly used HKG through five algorithms (NormFinder, geNorm, BestKeeper, &Delta, CT, coefficient of variation) and found that no universal HKG is suitable, since at least four HKG are necessary for proper normalization. Furthermore, according to the acceptable range of values, YWHAZ, GAPDH, GUSB, and 18S rRNA were the most stable reference genes at different pH. Our results will be helpful for future investigations focusing on the effect of altered microenvironment on cancer behavior, particularly on the effectiveness of anticancer therapies in acid conditions.

Published
2018
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19. Incorporation/Enrichment of 3D Bioprinted Constructs by Biomimetic Nanoparticles: Tuning Printability and Cell Behavior in Bone Models.

Author

Fischetti T, Borciani G, Avnet S, Rubini K, Baldini N, Graziani G, and Boanini E

Abstract

Reproducing in vitro a model of the bone microenvironment is a current need. Preclinical in vitro screening, drug discovery, as well as pathophysiology studies may benefit from in vitro three-dimensional (3D) bone models, which permit high-throughput screening, low costs, and high reproducibility, overcoming the limitations of the conventional two-dimensional cell cultures. In order to obtain these models, 3D bioprinting offers new perspectives by allowing a combination of advanced techniques and inks. In this context, we propose the use of hydroxyapatite nanoparticles, assimilated to the mineral component of bone, as a route to tune the printability and the characteristics of the scaffold and to guide cell behavior. To this aim, both stoichiometric and Sr-substituted hydroxyapatite nanocrystals are used, so as to obtain different particle shapes and solubility. Our findings show that the nanoparticles have the desired shape and composition and that they can be embedded in the inks without loss of cell viability. Both Sr-containing and stoichiometric hydroxyapatite crystals permit enhancing the printing fidelity of the scaffolds in a particle-dependent fashion and control the swelling behavior and ion release of the scaffolds. Once Saos-2 cells are encapsulated in the scaffolds, high cell viability is detected until late time points, with a good cellular distribution throughout the material. We also show that even minor modifications in the hydroxyapatite particle characteristics result in a significantly different behavior of the scaffolds. This indicates that the use of calcium phosphate nanocrystals and structural ion-substitution is a promising approach to tune the behavior of 3D bioprinted constructs.

Published
2023
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