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The assessment of the modulus of elasticity and compressive strength of masonry is a fundamental step in the seismic analysis of existing structures. In this paper, the representativeness of the values provided by flat-jack tests for tuff masonry is investigated through the analysis of a very large and homogeneous number of tests (635 double flat-jack tests). Data relate to existing buildings belonging to different historical and/or construction periods, located throughout the Campania region (Italy) in areas with different peculiarities. Results are compared with the values provided by Italian Building Code, containing ranges of the elastic modulus and compressive strength for different types of masonry. The values of flat-jack tests are then compared with laboratory tests available in the literature. Finally, comparisons with code equations are performed. It is shown that equations correlating the masonry compressive strength with the modulus of elasticity provide values larger than the mean of experimental data, whereas the equations correlating the masonry compressive strength with the strength of components provide lower values, if block and mortar strengths are varied within a probable and wide range.