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Although Blastocystis sp. is the most common enteric protozoan in human stools worldwide, various geographical areas remain to be investigated regarding the frequency and circulation of this parasite. Such is the case of some developing countries in Southeast Asia that exhibit a higher risk for parasitic infections due to unsanitary conditions. While several epidemiological surveys have been conducted, for instance, in Thailand, little or no data are available from neighboring countries, such as Vietnam. Therefore, in order to determine the prevalence and subtype (ST) distribution of Blastocystis sp. and to clarify the transmission of the parasite, the first molecular epidemiological survey ever conducted in this country was performed. For this purpose, a total of 310 stool specimens were collected from patients enrolled at the Family Hospital of Da Nang and then tested for the presence of Blastocystis sp. by real-time Polymerase Chain Reaction (qPCR), followed by subtyping of the isolates. The overall prevalence of the parasite reached 34.5% in this Vietnamese cohort. No significant association was found between parasite infection and gender, age, symptomatic status, contact with animals or source of drinking water. Out of the 107 positive patients, nearly half presented mixed infections. Therefore, some of the corresponding samples were reanalyzed by end-point PCR, followed by PCR products cloning and sequencing. Of the 88 total subtyped isolates, ST3 was predominant, followed by ST10, ST14, ST7, ST1, ST4, ST6 and ST8. Our study was, thus, the first to report ST8, ST10 and ST14 in the Southeast Asian population. The predominance of ST3 within this Vietnamese cohort, coupled with its low intra-ST genetic variability, reflected a large inter-human transmission, while ST1 transmission was suggested to be not only anthroponotic, but also likely correlated to animal or environmental sources. Strikingly, isolates considered of animal origin (ST6-ST8, ST10 and ST14) accounted for more than 50% of the subtyped isolates. These findings improved our knowledge of the epidemiology and circulation of Blastocystis sp. in Southeast Asia, and in particular, in Vietnam, and highlighted both a major burden of the parasite in this country and a high risk of zoonotic transmission, mainly from poultry and livestock.

A design of a modular antenna/detector array for the electron cyclotron emission (ECE) imaging system at the Korea Superconducting Tokamak Advanced Research (KSTAR) is proposed. The modular antenna/detector array is based on a unit antenna/detector module, which consists of an elliptical mini-lens, a dual-dipole antenna, an antenna balun, a low-noise amplifier, and a metal frame. The proposed modular antenna/detector array resolves the problem in the conventional antenna/detector array where one faulty channel requires the entire array to be removed for the service. With the proposed modular array, each channel module can be easily and independently removed and replaced without interference to the rest of the array, thus minimizing the interrupted service time for maintenance. Moreover, the unit channel modules can be efficiently updated under a variety of the tokamak operation conditions. The antenna/detector modules are optimized to have improved performance, and are tested in a W-band test setup, and consistently provide the gain increase by 10~20 dB as compared with the conventional antenna/detector array. A set of the proposed modular antenna/detector array is currently installed and tested in the KSTAR ECE imaging system, and will consistently produce the improved ECE imaging to monitor MHD instability activities under various plasma operation conditions.