In the present study, we report two new asexual fungal species (i.e., Discosia rhododendricola , Neopestalotiopsis rhododendricola ( Sporocadaceae ) and a new host for a previously described species (i.e., Diaporthe nobilis ; Diaporthaceae ). All species were isolated from Rhododendron spp. in Kunming, Yunnan Province, China. All taxa are described based on morphology, and phylogenetic relationships were inferred using a multigenic approach (LSU, ITS, RPB2 , TEF1 and TUB2 ). The phylogenetic analyses indicated that D. rhododendronicola sp. nov. is phylogenetically related to D. muscicola , and N. rhododendricola sp. nov is related to N. sonnaratae . Diaporthe nobilis is reported herein as a new host record from Rhododendron sp. for China, and its phylogeny is depicted based on ITS, TEF1 and TUB2 sequence data.