1. Corneal UV Protective Effects of a Topical Antioxidant Formulation: A Pilot Study on In Vivo Rabbits
- Author
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Germano Guerra, Marisa Palazzo, Lubomir Ondruška, Ciro Caruso, Michele Rinaldi, Francesco Vizzarri, Ciro Costagliola, Francesco Merolla, Luigi Pacente, Palazzo, M, Vizzarri, F, Ondruška, L, Rinaldi, M, Pacente, L, Guerra, G, Merolla, F, Caruso, C, Costagliola, C, Palazzo, M., Vizzarri, F., Ondruska, L., Rinaldi, M., Pacente, L., Guerra, G., Merolla, F., Caruso, C., and Costagliola, C.
- Subjects
0301 basic medicine ,Male ,Antioxidant ,medicine.medical_treatment ,alpha-Tocopherol ,Riboflavin ,Pilot Projects ,vitamin E ,Antioxidants ,lcsh:Chemistry ,chemistry.chemical_compound ,0302 clinical medicine ,Edema ,Cornea ,heterocyclic compounds ,Cornea protection ,D-α-tocopherol polyethylene glycol succinate (TPGS) ,Topic shielding formulation ,UV damage ,Vitamin E ,lcsh:QH301-705.5 ,Spectroscopy ,Chemistry ,digestive, oral, and skin physiology ,food and beverages ,General Medicine ,Computer Science Applications ,Radiation Injuries, Experimental ,medicine.anatomical_structure ,Rabbits ,medicine.symptom ,Swelling ,topic shielding formulation ,medicine.medical_specialty ,Ultraviolet Rays ,cornea protection ,Radiation-Protective Agents ,Polyethylene glycol ,Catalysis ,Article ,Inorganic Chemistry ,03 medical and health sciences ,In vivo ,Ophthalmology ,medicine ,Animals ,Physical and Theoretical Chemistry ,riboflavin ,Molecular Biology ,Organic Chemistry ,eye diseases ,030104 developmental biology ,lcsh:Biology (General) ,lcsh:QD1-999 ,030221 ophthalmology & optometry ,sense organs ,Corneal Injuries - Abstract
This study aimed to evaluate the protective effect of a topical antioxidant and ultraviolet (UV) shielding action formulation containing riboflavin and D-&alpha, tocopherol polyethylene glycol succinate (TPGS) vitamin E against corneal UV-induced damage in vivo rabbit eyes. In vivo experiments were performed using male albino rabbits, which were divided into four groups. The control group (CG) did not receive any UV irradiation, the first group (IG) was irradiated with a UV-B&minus, UV-A lamp for 30 min, the second (G30) and third (G60) groups received UV irradiation for 30 and 60 min, respectively, and were topically treated with one drop of the antioxidant and shielding formulation every 15 min, starting one hour before irradiation, until the end of UV exposure. The cornea of the IG group showed irregular thickening, detachment of residual fragments of the Descemet membrane, stromal fluid swelling with consequent collagen fiber disorganization and disruption, and inflammation. The cornea of the G30 group showed edema, a mild thickening of the Descemet membrane without fibrillar collagen disruption and focal discoloration, or inflammation. In the G60 group, the cornea showed a more severe thickening, a more abundant fluid accumulation underneath the Descemet membrane with focal detachment, and no signs of severe tissue alterations, as were recorded in the IG group. Our results demonstrate that topical application of eye drops containing riboflavin and TPGS vitamin E counteracts UV corneal injury in exposed rabbits.
- Published
- 2020