Search

In this paper, the flow characteristics of a continuous-type liquid–liquid pintle injector are described, focusing on the differential impact of a non-uniform center-flow distribution on single- and bi-injection methodologies as well as the cavitation effect on the spray angle. Using cold-flow experiments, jet-type flows of the center propellant caused by a non-uniform flow distribution were observed during a single injection. This resulted in an augmented pressure drop, as opposed to the flow characteristics of uniform single-film injection. By contrast, bi-injection modalities exhibited a substantial reduction in the pressure drop of the center propellant, underscoring a more equitable flow distribution. Moreover, the occurrence of cavitation in the center propellant was found to markedly affect the spray angle. By considering the injection exit area reduction caused by cavitation, the spray-angle prediction accuracy increased. The findings of this study are expected to reveal the interplay between flow distribution and pressure drop as well as that between cavitation and the spray angle in pintle injectors. Through this understanding, this study provides crucial considerations for the development of more efficient propulsion systems.

Protein synthesis is strictly regulated during replicative aging in yeast, but global translational regulation during replicative aging is poorly characterized. To conduct ribosome profiling during replicative aging, we collected a large number of dividing aged cells using a miniature chemostat aging device. Translational efficiency, defined as the number of ribosome footprints normalized to transcript abundance, was compared between young and aged cells for each gene. We identified more than 700 genes with changes greater than twofold during replicative aging. Increased translational efficiency was observed in genes involved in DNA repair and chromosome organization. Decreased translational efficiency was observed in genes encoding ribosome components, transposon Ty1 and Ty2 genes, transcription factor HAC1 gene associated with the unfolded protein response, genes involved in cell wall synthesis and assembly, and ammonium permease genes. Our results provide a global view of translational regulation during replicative aging, in which the pathways involved in various cell functions are translationally regulated and cause diverse phenotypic changes.