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9 results on '"Febrer Sendra, Begoña"'

1. Evaluation of Loop-Mediated Isothermal Amplification (LAMP) in Urine Samples for the Diagnosis of Imported Schistosomiasis

Author

Salas-Coronas, Joaquín, primary, Luzón-García, María Pilar, additional, Crego-Vicente, Beatriz, additional, Soriano-Pérez, Manuel Jesús, additional, Febrer-Sendra, Begoña, additional, Vázquez-Villegas, José, additional, Diego, Juan García-Bernalt, additional, Cabeza-Barrera, Isabel María, additional, Castillo-Fernández, Nerea, additional, Muro, Antonio, additional, Bargues, María Dolores, additional, and Fernández-Soto, Pedro, additional

Published
2023
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4. SMART-LAMP: A Smartphone-Operated Handheld Device for Real-Time Colorimetric Point-of-Care Diagnosis of Infectious Diseases via Loop-Mediated Isothermal Amplification

Author

García-Bernalt Diego, Juan, primary, Fernández-Soto, Pedro, additional, Márquez-Sánchez, Sergio, additional, Santos Santos, Daniel, additional, Febrer-Sendra, Begoña, additional, Crego-Vicente, Beatriz, additional, Muñoz-Bellido, Juan Luis, additional, Belhassen-García, Moncef, additional, Corchado Rodríguez, Juan M., additional, and Muro, Antonio, additional

Published
2022
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5. Colorimetric and Real-Time Loop-Mediated Isothermal Amplification (LAMP) for Detection of Loa loa DNA in Human Blood Samples

Author

Febrer-Sendra, Begoña, primary, Fernández-Soto, Pedro, additional, Crego-Vicente, Beatriz, additional, Diego, Juan García-Bernalt, additional, Ta-Tang, Thuy-Huong, additional, Berzosa, Pedro, additional, Nguema, Rufino, additional, Ncogo, Policarpo, additional, Romay-Barja, María, additional, Herrador, Zaida, additional, Benito, Agustín, additional, and Muro, Antonio, additional

Published
2022
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6. Detection of SARS-CoV-2 RNA in Urine by RT-LAMP: A Very Rare Finding

Author

García-Bernalt Diego, Juan, primary, Fernández-Soto, Pedro, additional, Muñoz-Bellido, Juan Luis, additional, Febrer-Sendra, Begoña, additional, Crego-Vicente, Beatriz, additional, Carbonell, Cristina, additional, López-Bernús, Amparo, additional, Marcos, Miguel, additional, Belhassen-García, Moncef, additional, and Muro, Antonio, additional

Published
2021
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7. Application of a Genus-Specific LAMP Assay for Schistosome Species to Detect Schistosoma haematobium x Schistosoma bovis Hybrids

Author

Crego-vicente, Beatriz, Fernández-soto, Pedro, Febrer-sendra, Begoña, García-bernalt Diego, Juan, Boissier, Jérôme, Angora, Etienne K., Oleaga, Ana, Muro, Antonio, Crego-vicente, Beatriz, Fernández-soto, Pedro, Febrer-sendra, Begoña, García-bernalt Diego, Juan, Boissier, Jérôme, Angora, Etienne K., Oleaga, Ana, and Muro, Antonio

Abstract

Schistosomiasis is a disease of great medical and veterinary importance in tropical and subtropical regions caused by different species of parasitic flatworms of the genus Schistosoma. The emergence of natural hybrids of schistosomes indicate the risk of possible infection to humans and their zoonotic potential, specifically for Schistosoma haematobium and S. bovis. Hybrid schistosomes have the potential to replace existing species, generate new resistances, pathologies and extending host ranges. Hybrids may also confuse the serological, molecular and parasitological diagnosis. Currently, LAMP technology based on detection of nucleic acids is used for detection of many agents, including schistosomes. Here, we evaluate our previously developed species-specific LAMP assays for S. haematobium, S. mansoni, S. bovis and also the genus-specific LAMP for the simultaneous detection of several Schistosoma species against both DNA from pure and, for the first time, S. haematobium x S. bovis hybrids. Proper operation was evaluated with DNA from hybrid schistosomes and with human urine samples artificially contaminated with parasites’ DNA. LAMP was performed with and without prior DNA extraction. The genus-specific LAMP properly amplified pure Schistosoma species and different S. haematobium-S. bovis hybrids with different sensitivity. The Schistosoma spp.-LAMP method is potentially adaptable for field diagnosis and disease surveillance in schistosomiasis endemic areas where human infections by schistosome hybrids are increasingly common

Published
2021
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