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The aim of the research was to find a zirconia treatment method that would reduce or minimize the transformation from the tetragonal phase to the monoclinic phase. Background: Yttria-stabilized zirconia is increasingly chosen for the base of permanent prosthetic restorations. To achieve a good bond between the prosthetic cup and the veneer material, the material must be treated to achieve surface development. This is a mechanical process, during which an unfavorable transformation from the tetragonal into the monoclinic phase takes place, which leads to the weakening of the internal structure of zirconium dioxide, and later damages the prosthetic restoration. Methods: The tested material consisted of cylindrical samples of 3Y-TZP CeramillZi zirconium oxide, which were sintered after cutting out from the block. After sintering, the samples were subjected to the following types of processing: laser structuring, chemical etching and plasma etching. After the surface treatments, the samples were subjected to diffraction tests to determine the phase composition. Next, the wettability was tested to determine the surface free energy. Results: On the basis of the conducted tests, it was noticed that the applied treatments caused a phase transformation from the tetragonal to the monoclinic phase. After the process of chemical etching, the range of the monoclinic phase for the sample was 5%; after plasma etching, it was 8%, and after laser structuring, it was 2%. In addition, post-surface free energy studies have shown that zirconia is wetted better with an apolar than a polar liquid. Conclusions: The obtained results indicate that the transformation was minimized with the treatments we applied; that is why they are called non-invasive methods. According to the literature data, depending on the parameters of the sandblasting process, the percentage of the monoclinic phase in the treated surfaces ranges from 22% to 52%, which confirms the above-mentioned conclusion.

Recent studies have focused on the development of bio-based products from sustainable resources using green extraction approaches, especially nanocellulose, an emerging nanoparticle with impressive properties and multiple applications. Despite the various sources of cellulose nanofibers, the search for alternative resources that replace wood, such as Lygeum spartum, a fast-growing Mediterranean plant, is crucial. It has not been previously investigated as a potential source of nanocellulose. This study investigates the extraction of novel cellulose micro/nanofibers from Lygeum spartum using a two-step method, including both alkali and mechanical treatment as post-treatment with ultrasound, as well as homogenization using water and dilute alkali solution as a solvent. To determine the structural properties of CNFs, a series of characterization techniques was applied. A significant correlation was observed between the Fourier transform infrared (FTIR) spectroscopy and X-ray diffraction (XRD) results. The FTIR results revealed the elimination of amorphous regions and an increase in the energy of the H-bonding modes, while the XRD results showed that the crystal structure of micro/nanofibers was preserved during the process. In addition, they indicated an increase in the crystallinity index obtained with both methods (deconvolution and Segal). Thermal analysis based on thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC) confirmed improvement in the thermal properties of the isolated micro/nanofibers. The temperatures of maximum degradation were 335 °C and 347 °C. Morphological analysis using a scanning electron microscope (SEM) and atomic force microscope (AFM) showed the formation of fibers along the axis, with rough and porous surfaces. The findings indicate the potential of Lygeum spartum as a source for producing high-quality micro/nanofibers. A future direction of study is to use the cellulose micro/nanofibers as additives in recycled paper and to evaluate the mechanical properties of the paper sheets, as well as investigate their use in smart paper.

The literature analysis did not indicate any studies on fluorination tests of carbon nanocomposite coatings doped with transition metals in a form of nanocrystalline metal carbide in amorphous carbon matrix (nc-MeC/a-C). As a model coating to investigate the effect of fluorination in a tetrafluoromethane (CF4) atmosphere, a nanocomposite carbon coating doped with chromium-forming nanocrystals of chromium carbides in a-C matrix (nc-CrC/a-C) produced by magnetron sputtering from graphite targets and using a Pulse-DC type medium frequency power supply was chosen. After the deposition of the gradient chromium carbonitride (CrCN) adhesive sublayer, the fluorination of the main coating was conducted in a reactive mode in an (Ar + CF4) atmosphere at various CF4 content. It was observed that the presence of CF4 in the atmosphere resulted in a reduced amount of chromium carbides formed in favor of chromium fluorides. Thus far, this is an observation that seems unnoticed by the carbon coatings researchers. Fluorine was assumed to bond much more readily to carbon than to chromium, due to the stability of tetrafluoromethane (CF4). The opposite seems to be true. The mechanical properties (nano-hardness and Young’s modulus) and tribological properties in the ‘pin-on-disc’ friction pair are presented, along with the analysis of bonds occurring between chromium, carbon, and fluorine by means of X-ray photoelectron spectroscopy (XPS).

Despite significant advancements in the field of oncology, cancers still pose one of the greatest challenges of modern healthcare. Given the cytoskeleton’s pivotal role in regulating mechanisms critical to cancer development, further studies of the cytoskeletal elements could yield new practical applications. Septins represent a group of relatively well-conserved GTP-binding proteins that constitute the fourth component of the cytoskeleton. Septin 9 (SEPT9) has been linked to a diverse spectrum of malignancies and appears to be the most notable septin member in that category. SEPT9 constitutes a biomarker of colorectal cancer (CRC) and has been positively correlated with a high clinical stage in breast cancer, cervical cancer, and head and neck squamous cell carcinoma. SEPT9_i1 represents the most extensively studied isoform of SEPT9, which substantially contributes to carcinogenesis, metastasis, and treatment resistance. Nevertheless, the mechanistic basis of SEPT9_i1 oncogenicity remains to be fully elucidated. In this review, we highlight SEPT9’s and SEPT9_i1’s structures and interactions with Hypoxia Inducible Factor α (HIF-1 α) and C-Jun N-Terminal Kinase (JNK), as well as discuss SEPT9_i1’s contribution to aneuploidy, cell invasiveness, and taxane resistance—key phenomena in the progression of malignancies. Finally, we emphasize forchlorfenuron and other septin inhibitors as potential chemotherapeutics and migrastatics.

Exportin 1 (XPO1) is a crucial molecule of nucleocytoplasmic transport. Among others, it exports molecules important for oncogenesis from the nucleus to the cytoplasm. The expression of XPO1 is increased in numerous malignancies, which contributes to the abnormal localization of tumor suppressor proteins in the cytoplasm and subsequent cell cycle dysregulation. Selective inhibitors of nuclear export (SINEs) are novel anticancer agents that target XPO1, arrest tumor suppressor proteins in the nucleus, and induce apoptosis in cancer cells. Selinexor, a first-in-class SINE, has already been approved for the treatment of relapsed/refractory multiple myeloma and relapsed/refractory diffuse large B cell lymphoma not otherwise specified. It has also been proven effective in relapsed/refractory and previously untreated acute myeloid leukemia patients. In addition, numerous studies have yielded promising results in other malignancies of the hematopoietic system and solid tumors. However, future clinical use of selinexor and other SINEs may be hampered by their significant toxicity.

Chitin, a ubiquitous biopolymer, holds paramount scientific and economic significance. Historically, it has been primarily isolated from marine crustaceans. However, the surge in demand for chitin and the burgeoning interest in biopolymers have necessitated the exploration of alternative sources. Among these methods, the mulberry silkworm (Bombyx mori) has emerged as a particularly intriguing prospect. To isolate chitin from Bombyx mori, a chemical extraction methodology was employed. This process involved a series of meticulously orchestrated steps, including Folch extraction, demineralization, deproteinization, and decolorization. The resultant chitin was subjected to comprehensive analysis utilizing techniques such as attenuated total reflectance–Fourier transform infrared spectroscopy (ATR-FTIR), 13C nuclear magnetic resonance (NMR) spectroscopy, and wide-angle X-ray scattering (WAXS). The obtained results allow us to conclude that the Bombyx mori represents an attractive alternative source of α-chitin.

Sustainable development is the subject of many economic analyses, but so far no attempt has been made to identify the main mechanism of interdependence between sustainable energy development and sustainable economic development in the second decade of the 21st century. The particular role of energy in achieving the Sustainable Development Goals is due to the fact that the production, supply and use of energy underpin economic growth. The article fills this research gap and spawns both a better understanding of the essence of sustainable development as well as practical conclusions. The aim is to assess sustainable energy development and sustainable economic development in EU member states and to determine the correlation between the two in the EU. Substantive and formal methods were used to select diagnostic variables, including: the parametric method, the standardized sums method, and correlation analysis. The analysis period covers the years 2014–2021. The conducted research demonstrated a significant variation in the level of sustainable energy development and sustainable economic development among EU countries.

The aim of this study was to obtain a series of activated carbon samples by the chemical activation of low-rank coal. The precursor was impregnated with a NaOH solution. Activated carbons were characterized by determining their textural parameters and content of surface oxygen functional groups and by using an elemental analysis. The carbons were tested as potential adsorbents for the removal of liquid pollutants represented by rhodamine B. The effectiveness of rhodamine B removal from water solutions depended on the initial concentration of the dye, the mass of rhodamine B, and the pH and temperature of the reaction. The isotherm examination followed the Langmuir isotherm model. The maximum adsorption capacity of the rhodamine B was 119 mg/g. The kinetic investigation favored the pseudo-second-order model, indicating a chemisorption mechanism. The thermodynamic assessment indicated spontaneous and endothermic adsorption, with decreased randomness at the solid–liquid interface. The experiment revealed that a 0.1 M HCl solution was the most effective regenerative agent.

The main objective of this study was to discover new packaging materials that could integrate one of the most expected properties, such as UV protection, with a self-cleaning ability defined as photocatalytic performance. Accordingly, new hybrid additives were used to transform LDPE films into materials with complex performance properties. In this study, titanium dioxide–lignin (TL) hybrid systems with a weight ratio of inorganic to organic precursors of 5-1, 1-1, and 1-5 were prepared using a mechanical method. The obtained materials and pristine components were characterized using measurement techniques and research methods, such as Fourier-transform infrared spectroscopy (FTIR), thermal stability analysis (TGA/DTG), measurement of the electrokinetic potential as a function of pH, scanning electron microscopy (SEM), and particle size distribution measurement. It was found that hydrogen bonds were formed between the organic and inorganic components, based on which the obtained systems were classified as class I hybrid materials. In the next step, inorganic–organic hybrid systems and pristine components were used as fillers for a low-density polyethylene (LDPE) composite, 5 and 10% by weight, in order to determine their impact on parameters such as tensile elongation at break. Polymer composites containing titanium dioxide in their matrix were then subjected to a test of photocatalytic properties, based on which it was found that all materials with TiO2 in their structure exhibit photocatalytic properties, whereby the best results were obtained for samples containing the TiO2–lignin hybrid system (1-1). The mechanical tests showed that the thin sheet films had a strong anisotropy due to chill-roll extrusion, ranging from 1.98 to 3.32. UV–Vis spectroscopy revealed four times higher light absorption for composites in which lignin was present than for pure LDPE, in the 250–450 nm range. On the other hand, the temperature at 5% and 30% weight loss revealed by TGA testing increased the highest performance for LDPE/TiO2 materials (by 20.4 °C and 8.7 °C, respectively).

In this work, Ti-incorporated carbon coatings were used as substrates for modification with one- and two-component self-assembled monolayers of organosilane compounds using a polydimethylsiloxane (PDMS) stamp. This enabled the selective functionalization of surfaces with micrometric dimensions. The topography of the modified surfaces was defined using an atomic force microscope (AFM). The effectiveness of the modification was confirmed by measurements of the water contact angle and surface free energy using the Oss and Good method. Using a T-23 microtribometer with counterparts in the shape of balls that were made of steel, silicon nitride (Si3N4), and zirconium dioxide (ZrO2), the tribological properties of the obtained coatings were tested. These investigations showed that modification by using a PDMS stamp makes it possible to produce two-component ultrathin silane layers on Ti-containing carbon substrates. Two-component organosilane layers had higher hydrophobicity, a lower friction coefficient, and a smaller width of wear tracks than the one-component analogs. It was also found that the work of adhesion of the created surfaces had a significant influence on the value of the friction coefficient and the percentage value of the growth inhibition of bacteria.

Atherosclerosis affects human health in many ways, leading to disability or premature death due to ischemic heart disease, stroke, or limb ischemia. Poststenotic blood flow disruption may also play an essential role in artery wall impairment linked with hemolysis related to shear stress. The maximum shear stress in the atherosclerotic plaque area is the main parameter determining hemolysis risk. In our work, a 3D internal carotid artery model was built from CT scans performed on patients qualified for percutaneous angioplasty due to its symptomatic stenosis. The obtained stenosis geometries were used to conduct a series of computer simulations to identify critical parameters corresponding to the increase in shear stress in the arteries. Stenosis shape parameters responsible for the increase in shear stress were determined. The effect of changes in the carotid artery size, length, and degree of narrowing on the change in maximum shear stress was demonstrated. Then, a correlation for the quick initial diagnosis of atherosclerotic stenoses regarding the risk of hemolysis was developed. The developed relationship for rapid hemolysis risk assessment uses information from typical non-invasive tests for treated patients. Practical guidelines have been developed regarding which stenosis shape parameters pose a risk of hemolysis, which may be adapted in medical practice.

In this research, we aimed to design an eco-efficient composite based on alkali-activated materials (AAMs) with self-cleaning properties for sustainable construction. Significant emphasis was placed on determining the role of the type of precursor, the amount of sodium silicate, and the addition of titanium dioxide on the rheological and mechanical properties of AAMs. An important aspect of the research was the modification of AAM with titanium dioxide to obtain the self-cleaning properties. Titanium dioxide, thanks to its photocatalytic properties, enables the reduction of organic pollutants and nitrogen oxides in the urban atmosphere and promotes the cleaning of material surfaces. Blast furnace slag (BFS) was used as the source material, which was then substituted in subsequent formulations with metakaolinite at 50% and fly ash and zeolite at 30%. The best-activated AAMs, in which blast furnace slag and its mixture with metakaolinite were used as precursors, achieved compressive strengths of 50 MPa. BFS mixtures with pozzolans were more difficult to polymerize, although their final strengths were still relatively high, in the range of 33–37 MPa. Adding titanium dioxide (T) improved the final strengths and slightly lowered the heat of hydration and spreading of the AAM mortars. The best self-cleaning properties were achieved with composites that comprised a mixture of blast furnace slag, fly ash, and 2% titanium dioxide.

Sustainable development has been the focus of many analyses in economics. Nevertheless, only a few attempts have so far been made to identify the underlying relationship between sustainable energy development and sustainable social development. This article fills this research gap and enables a better understanding of the essence of sustainable development to help draw applicable conclusions. The aim is to assess sustainable energy and social development in EU countries and to examine their shared relationship in this area. To this end, this study uses a taxonomic method and correlation analysis. The analysis period covers the years 2014–2020. The research builds directly on the assumptions of the concept of sustainable development, with particular emphasis on energy and social aspects. The article is composed of two parts. The first, based on a literature review, discusses the essence of sustainable energy development and social sustainability. The second outlines the research methodology, including the underpinnings of synthetic measures and the results of the conducted empirical research.

Immunization against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has significantly limited the spread of coronavirus disease 2019 (COVID-19) and reduced the associated complications, especially mortality. To prolong immunity, an immune booster was implemented. We evaluated the role of SARS-CoV-2 infection history in the vaccination schedules of kidney and liver transplant recipients and patients with chronic kidney disease (CKD). To this end, we retrospectively analyzed the data of 78 solid organ transplantation (SOT) recipients and 40 patients with immunoglobulin A (IgA) nephropathy as representatives of the CKD group. Patients received two or three doses of the BNT162b2 vaccine. At the follow-up, antibody (Ab) titer, graft function, COVID-19 history, and patients’ clinical condition were assessed. Ab level was higher after two doses in patients with a COVID-19 history over three doses in patients with no COVID-19 history. Compared to three doses, subjects who were administered two doses had a longer median time to infection. Positive antibodies, in response to the third dose, were not observed in up to 8.4% of SOT patients. The results show that the vaccination schedule should take into account the vaccine response rate and COVID-19 history. So-called hybrid immunity appears to be most efficient at providing humoral responses against SARS-CoV-2 infection in immunocompromised patients.

Nanoparticles are extremely promising components that are used in diagnostics and medical therapies. Among them, silica nanoparticles are ultrafine materials that, due to their unique physicochemical properties, have already been used in biomedicine, for instance, in cancer therapy. The aim of this study was to investigate the cytotoxicity of three types of nanoparticles (SiO2, SiO2-SH, and SiO2-COOH) in relation to red blood cells, as well as the impact of silicon dioxide nanoparticles on biological membranes and liposome models of membranes. The results obtained prove that hemolytic toxicity depends on the concentration of nanoparticles and the incubation period. Silica nanoparticles have a marginal impact on the changes in the osmotic resistance of erythrocytes, except for SiO2-COOH, which, similarly to SiO2 and SiO2-SH, changes the shape of erythrocytes from discocytes mainly towards echinocytes. What is more, nanosilica has an impact on the change in fluidity of biological and model membranes. The research gives a new view of the practical possibilities for the use of large-grain nanoparticles in biomedicine.

Bacterial cellulose is a unique biopolymer that has found numerous biomedical applications, such as being an excellent wound-dressing material or a carrier for delivering active compounds. The purpose of this study was to analyze the ability of modified bacterial cellulose (BC) using low-pressure Ar plasma to control the release of glycoside hydrolases with antibiofilm activity, namely PelAh and PslGh, from Pseudomonas aeruginosa. The chemical composition and morphology of the BC surfaces were characterized using photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). The analyses revealed significant changes in the chemical composition of the BC surface due to the introduction of charged functional groups and the conversion of its well-ordered structure into a more amorphous form. The release profiles of enzymes from both forms of the carrier were different and depended on their structural properties. However, a significant impact of BC modification on protein release behavior from the carrier was observed only for PslGh. Both enzymes, when immobilized on pristine and argon plasma-modified BC, retained their ability to effectively reduce biofilm levels, similarly to their soluble form. Ar plasma-modified BC with immobilized specific hydrolases can be used as an effective tool for inhibiting P. aeruginosa biofilm development.

Recurrent implantation failure (RIF) is a global health issue affecting a significant number of infertile women who undergo in vitro fertilization (IVF) cycles. Extensive vasculogenesis and angiogenesis occur in both maternal and fetal placental tissues, and vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF) family molecules and their receptors are potent angiogenic mediators in the placenta. Five single nucleotide polymorphisms (SNPs) in the genes encoding angiogenesis-related factors were selected and genotyped in 247 women who had undergone the ART procedure and 120 healthy controls. Genotyping was conducted by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). A variant of the kinase insertion domain receptor (KDR) gene (rs2071559) was associated with an increased risk of infertility after adjusting for age and BMI (OR = 0.64; 95% CI: 0.45–0.91, p = 0.013 in a log-additive model). Vascular endothelial growth factor A (VEGFA) rs699947 was associated with an increased risk of recurrent implantation failures under a dominant (OR = 2.34; 95% CI: 1.11–4.94, padj. = 0.022) and a log-additive model (OR = 0.65; 95% CI 0.43–0.99, padj. = 0.038). Variants of the KDR gene (rs1870377, rs2071559) in the whole group were in linkage equilibrium (D’ = 0.25, r2 = 0.025). Gene–gene interaction analysis showed the strongest interactions between the KDR gene SNPs rs2071559–rs1870377 (p = 0.004) and KDR rs1870377–VEGFA rs699947 (p = 0.030). Our study revealed that the KDR gene rs2071559 variant may be associated with infertility and rs699947 VEGFA with an increased risk of recurrent implantation failures in infertile ART treated Polish women.

There has been growing interest in using underground locations for applications in various fields, including research. In Poland, for several years, attempts have been made to build an underground laboratory. For this purpose, selecting an appropriate location requires a detailed analysis of the level of natural radioactivity. The present study presents detailed characteristics of the natural background radiation in close vicinity to shaft L-VI of the Lubin mine, at the depth of 910 m (2275 m w.e.). The in situ measurement of the photon flux in the 7–3150 keV energy range was equal to 8.08 ± 0.90 cm−2s−1, and the gamma-ray dose rate of 0.070 ± 0.010 µSv/h with the highest contribution from 40K and 214B isotopes. The thermal neutron flux measured using helium counters was equal to 4.2 ± 0.9 × 10−6 cm−2s−1. The radon concentration in the air measured with the RAD7 monitor showed low values ranging from 0 to 15.3 Bq/m3. Laboratory measurements of rocks using alpha and gamma spectrometry techniques showed a significant variation in the concentration of 226Ra and 234,238U isotopes, and the highest concentration values were recorded for shales. The 40K, 234,238U and 226Ra isotopes make the greatest contribution to the natural radioactivity of analyzed rocks.

This study addresses the relationship between accounting quality and audit attributes (i.e., audit quality, auditor industry specialization, audit concentration, and audit fees) with companies’ SPCRs listed on the Iraqi Stock Exchange. A multivariate regression model was used to test the hypotheses. The research hypotheses were tested using a sample of 210 observations of the listed Iraqi firms from 2013 to 2018 and a multiple regression model based on the random-effects model’s panel data technique. The findings indicate a negative and significant relationship between the accounting quality, audit, auditor industry specialization and SPCRs. Results also conveyed a meaningful and positive association between stock price crash risk (SPCR) and audit fees. The results did not confirm the relationship between corporate governance and audit concentration with SPCR. The primary research model was tested with additional methods (t + 1, fixed effects, ordinary least squares). Since this is the first study addressing this issue in the emerging markets, it provides users, analysts, and legal entities with helpful information about audit attributes that significantly affect SPCR. These results also contribute to developing science and knowledge in this field and fill the literature gap.

Introduction: Some evidence indicates that the improper trophoblast invasion of maternal spiral arteries could be caused by an imbalance between matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs), leading to preeclampsia (PE) development. This study aimed to assess the potential role of MMP1, MMP9, TIMP1 and TIMP2 gene polymorphisms in the pathogenesis of PE. Materials and methods: A total of 308 Polish women, 115 preeclamptic (55 with early-onset preeclampsia [EOPE], 60 with late-onset preeclampsia [LOPE]) and 193 healthy pregnant women, all of Caucasian origin, were recruited to the study. PE was diagnosed following the ACOG criteria. The polymorphic variants of the MMP-TIMP pathway (MMP1 rs1799750, MMP9 rs17576, MMP9 rs17577, TIMP1 rs4898, TIMP2 rs2277698, TIMP2 rs55743137) were genotyped by polymerase chain reaction and restriction fragment length polymorphism. Results: Analyzing all SNPs in the MMP-TIMP pathway, no significant differences in allele frequencies between preeclamptic women and controls were observed. However, comparing the EOPE and LOPE groups with each other, we observed a statistically significant difference between them for the TIMP1 rs4898 variant. In the whole group of 308 women, the mean placenta weight was the lowest in carriers of the rs4898 CC genotype. Post hoc analysis revealed significant differences between CC-CT (p = 0.0209) and CC-TT (p = 0.0469). Additionally, during allele analysis, a statistically significant difference in the mean placenta weight (for C allele 529.32 ± 157.11 g, for T allele 560.24 ± 162.24 g, p = 0.021) was also observed. Conclusion: Our findings suggest a relationship between TIMP1 rs4898 (372T > C) polymorphism and increased risk of early-onset preeclampsia in a population of pregnant Polish women. Our data suggest that the TIMP1 rs4898 C allele might be associated with increased risk for early-onset, but not for late-onset preeclampsia. To evaluate the role of the TIMP1 polymorphic variants in the etiopathology of preeclampsia, further studies with a larger sample size are needed.

Tomato-infecting viruses have been considered as a serious threat to tomato crops in Poland. Therefore, during 2014–2021, 234 tomato samples delivered directly by greenhouse tomato growers to Plant Disease Clinic of IPP-NRI were tested. Eight virus species: pepino mosaic virus (PepMV), tomato yellow ring orthotospovirus (TYRV), tomato spotted wilt orthotospovirus (TSWV), potato virus Y (PVY), cucumber mosaic virus (CMV), tomato black ring virus (TBRV) and tomato mosaic virus (ToMV) were detected in single or mixed infection in 89 samples. The presence of TYRV was established for the first time in Poland in 2014. Since then, its presence has been observed in single and mixed infection with TSWV and CMV. Here, we analysed the genetic variability of TYRV population based on complete nucleocapsid (N) protein gene sequence of 55 TYRV isolates. Maximum-likelihood reconstruction revealed the presence of three distinct, well-supported phylogroups. Moreover, the effect of host species on virus diversity was confirmed. Therefore, RT-LAMP assay was developed for the rapid and efficient detection of TYRV isolates that can be implemented in field and greenhouse conditions.

(1) Background: The present study aims to report the side effects of vaccination against coronavirus disease 2019 (COVID-19) among patients with multiple sclerosis (MS) who were being treated with disease-modifying therapies (DMTs) in Poland. (2) Methods: The study included 2261 patients with MS who were being treated with DMTs, and who were vaccinated against COVID-19 in 16 Polish MS centers. The data collected were demographic information, specific MS characteristics, current DMTs, type of vaccine, side effects after vaccination, time of side-effect symptom onset and resolution, applied treatment, relapse occurrence, and incidence of COVID-19 after vaccination. The results were presented using maximum likelihood estimates of the odds ratio, t-test, Pearson’s chi-squared test, Fisher’s exact p, and logistic regression. The statistical analyses were performed using STATA 15 software. (3) Of the 2261 sampled patients, 1862 (82.4%) were vaccinated with nucleoside-modified messenger RNA (mRNA) vaccines. Mild symptoms after immunization, often after the first dose, were reported in 70.6% of individuals. Symptoms included arm pain (47.5% after the first dose and 38.7% after the second dose), fever/chills/flu-like symptoms (17.1% after the first dose and 20.5% after the second dose), and fatigue (10.3% after the first dose and 11.3% after the second dose). Only one individual presented with severe side effects (pro-thrombotic complications) after vaccination. None of the DMTs in the presented cohort were predisposed to the development of side effects. Nine patients (0.4%) had a SARS-CoV-2 infection confirmed despite vaccination. (4) Conclusions: Vaccination against SARS-CoV-2 is safe for people with MS who are being treated with DMTs. Most adverse events following vaccination are mild and the acute relapse incidence is low.

Epigenetic modifications play a special role in the male infertility aetiology. Published data indicate the link between sperm quality and sperm chromatin protamination. This study aimed to determine the relationship between methylation (5mC) and hydroxymethylation (5hmC) in sperm DNA, with respect to sperm chromatin protamination in three subpopulations of fertile normozoospermic controls and infertile patients with oligo-/oligoasthenozoospermia. For the first time, a sequential staining protocol was applied, which allowed researchers to analyse 5mC/5hmC levels by immunofluorescence staining, with a previously determined chromatin protamination status (aniline blue staining), using the same spermatozoa. TUNEL assay determined the sperm DNA fragmentation level. The 5mC/5hmC levels were diversified with respect to chromatin protamination status in both studied groups of males, with the highest values observed in protaminated spermatozoa. The linkage between chromatin protamination and 5mC/5hmC levels in control males disappeared in patients with deteriorated semen parameters. A relationship between 5mC/5hmC and sperm motility/morphology was identified in the patient group. Measuring the 5mC/5hmC status of sperm DNA according to sperm chromatin integrity provides evidence of correct spermatogenesis, and its disruption may represent a prognostic marker for reproductive failure.

This article presents a comparative analysis of bacterial cellulose membranes synthesized by several strains of the Komagataeibacter genus in terms of their specific physical, physico-chemical, and mechanical properties. Herein, the aim was to choose the most suitable microorganisms producing cellulosic materials with the greatest potential for the fabrication of bio-inspired nanocomposites. The selection was based on three main steps, starting from the evaluation of BNC biosynthetic efficiency with and without the addition of ethanol, followed by the assessment of mechanical breaking strength, and the physical parameters (compactness, structural integrity, appearance, and thickness) of the obtained biological materials. Ultimately, based on the performed screening procedure, three efficiently growing strains (K. hansenii H3 (6Et), K. rhaeticus K4 (8Et), and Komagataeibacter sp. isolated from balsamic vinegar (12Et)) were chosen for further modifications, enabling additional cellulose functionalization. Here, supplementation of the growth medium with five representative polymeric compounds (citrus/apple pectin, wheat starch, polyvinyl alcohol, polyethylene glycol) led to significant changes in BNC properties, especially dye loading abilities, mechanical strength, and water adsorption/retention capacities. The resulting nanocomposites can be potentially useful in various fields of medicine and industry, and in the future, they may become a practical and cost-effective competitor against commercial biomaterials currently available on the market.

In recent years, increasing attention has been paid to the durability of building materials, including those based on cementitious binders. Important aspects of durability include the increase of the strength of the cement matrix and enhancement of material resistance to external factors. The use of nanoadditives may be a way to meet these expectations. In the present study, zinc, titanium and copper oxides, used in single and binary systems (to better the effect of their performance), were applied as additives in cement mortars. In the first part of this work, an extensive physicochemical analysis of oxides was carried out, and in the second, their application ranges in cement mortars were determined. The subsequent analyses were employed in determining the physicochemical properties of pristine oxides: Fourier transform infrared spectroscopy (FTIR), energy dispersive X-ray fluorescence (EDXRF), scanning electron microscopy (SEM), measurement of the particle size distribution, as well as zeta potential measurement depending on the pH values. Influence on selected physicomechanical parameters of the cement matrix and resistance to the action of selected Gram-positive and Gram-negative bacteria and fungi were also examined. Our work indicated that all nanoadditives worsened the mechanical parameters of mortars during the first 3 days of hardening, while after 28 days, an improvement was achieved for zinc and titanium(IV) oxides. Binary systems and copper(II) oxide deteriorated in strength parameters throughout the test period. In contrast, copper(II) oxide showed the best antibacterial activity among all the tested oxide systems. Based on the inhibitory effect of the studied compounds, the following order of microbial susceptibility to inhibition of growth on cement mortars was established (from the most susceptible, to the most resistant): E. coli < S. aureus < C. albicans < B. cereus = P. aeruginosa < P. putida.

Underground locations can be used in various ways for scientific and economic purposes. One of the main factors influencing the safety level in the underground mine workings is natural radioactivity. The article presents research carried out on the natural radioactivity in shallow mine workings at the GIG Experimental Mine ‘Barbara’. The description of the natural radiation includes radon determination in the air, in situ gamma spectrometry, neutron flux measurements, and laboratory measurements of 226,228Ra, 40K, and 234,238U isotopes using gamma and alpha spectrometry techniques. In the measurement chamber at the depth 46 m (122 m w.e.) in the sandstone layer, the photon flux registered at the 7–3150 keV energy range is equal to 17.6 ± 1.9 cm−2s−1, the gamma-ray dose rate is 0.200 ± 0.029 µSv/h, and the thermal neutron flux is equal to (8.6 ± 1.1) × 10−6 cm−2s−1. After closing the measurement chamber and turning off ventilation, a significant ingrowth of 222Rn content was observed, reaching the value of 4040 ± 150 Bq/m3. An increased gamma-ray flux and thermal neutron flux were observed in the investigated location.

The BNT162b2 vaccine is reportedly effective in preventing severe disease in more than 90% of the general population, but its efficacy in transplant recipients remains controversial. We aimed to determine the immune response to the BNT162b2 vaccine in kidney (KTRs) and liver transplant recipients (LTRs). In this retrospective cohort study, we included randomly 65 KTRs and 65 LTRs, who received two 30 μg doses of BNT162b2 vaccine in 3-to6-week intervals. We analyzed the anti-SARS-CoV-2 spike protein IgG antibody (anti-S1 Ab) titer, biochemical liver and renal tests, immunosuppressive drug trough level, and clinical follow up 4–6 weeks after the first dose and 4–8 weeks after the second dose. The level of protective antibodies was 57.1% in KTRs and 88.9% in LTRs after the second dose. The anti-S1 Ab response was significantly associated with sex, age, and history of COVID-19. A tacrolimus dose at vaccination but not its trough level was significantly correlated with the increase in anti-S1 Ab titer after the second vaccine dose in LTRs. Rejection episodes did not occur after vaccination. Our results showed a higher than previously reported humoral response to the BNT162b2 vaccine in KTRs and LTRs, which was dependent upon age, type of transplanted organ, and immunosuppression.

The purpose of this paper is a multidimensional assessment of the diversification of economic development in EU countries in the context of the progress in the implementation of the concept of sustainable development in 2014 and 2019. The issues discussed in this article are topical and important, given that the spatial disparity of economic development in the EU has never been so pronounced as it is today. While there is a wealth of studies on economic development in the literature, research on the comprehensive approach to this problem in the relation of sustainable development appears to be rather scarce. The article emphasizes the role of energy in economic growth. Authors used taxonomic measures. They were constructed on the basis of selected methods of multidimensional comparative analysis. By using the Hellwig method and the TOPSIS method, taxonomic measures were constructed, and linear ordering of the EU countries was carried out. In addition, using the so-called threshold method, the clustering of EU countries was carried out. The analysis involved 27 EU countries. The conducted research revealed significant disproportions between the respective EU countries in terms of the level of economic development in the relation of implementing the concept of sustainable development. It seems justified to take action aimed at eliminating the differences between the countries in the analyzed aspect. It is necessary both to intensify efforts at the level of individual Member States and the EU, oriented towards pro-development activities.

Currently, infertility affects 8–12% of reproductive age couples worldwide, a problem that also affects women suffering from recurrent implantation failure (RIF). RIF is a complex condition resulting from many physiological and molecular mechanisms involving dynamic endometrium–blastocyst interaction. The most important are the endometrial receptivity process, decidualization, trophoblast invasion, and blastocyst nesting. Although the exact multifactorial pathogenesis of RIF remains unclear, many studies have suggested the association between hormone level imbalance, disturbances of angiogenic and immunomodulatory factors, certain genetic polymorphisms, and occurrence of RIF. These studies were performed in quite small groups. Additionally, the results are inconsistent between ethnicities. The present review briefly summarizes the importance of factors involved in RIF development that could also serve as diagnostic determinants. Moreover, our review could constitute part of a new platform for discovery of novel diagnostic and therapeutic solutions for RIF.

Graphene has been considered as a material that may overcome the limitations of polymer semi-permeable membranes in water treatment technology. However, monolayer graphene still suffers from defects that cause leakage. Here, we report a method of sealing defects in graphene transferred onto porous polymer substrate via reduced graphene oxide (rGO). The influence of various reducing agents (e.g., vitamin C, hydrazine) on the properties of rGO was investigated by SEM, Raman, FTIR, and XRD. Subsequently, membranes based on graphene/reduced graphene oxide were tested in a forward osmosis system using sodium chloride (NaCl). The effect of the effectiveness of the reduction of graphene oxide, the type and number of attached groups, the change in the distance between the rGO flakes, and the structure of this material were examined in terms of filtration efficiency. As a result, semi-permeable centimetre-scale membranes with ion blocking efficiency of up to 90% and water flux of 20 mL h−1 m−2 bar−1 were proposed.

Natural radioactivity in underground locations is the main parameter for the safety of work (occupational hazards) and for the success of experiments in physics or biology requiring unique conditions. The characterization of natural prominence was carried out in the Conceptual Lab development in one of KGHM deep copper mines co-ordinated by KGHM Cuprum R&D. Natural radioactivity studies were performed and included in situ gamma spectrometry, neutron flux measurements, radon concentration, and alpha and gamma laboratory spectrometry measurements of rock samples. At a depth of 1014.4 m (2941.8 m w.e.) within the anhydrite layer, a neutron flux of 2.0 ± 0.2 × 10−6 cm−2 s−1, a gamma-ray dose of 0.008 ± 0.001 μSv/h, a photon flux density of 0.64 ± 0.20 cm−2 s−1, and a radon concentration of 6.6 Bq/m3 were determined. Laboratory analyses of 226,228Ra, 40K, and 238,234U concentrations in collected rock samples showed low values. The exceptionally low level of natural radioactivity in the Polkowice-Sieroszowice mine makes this location a unique place for scientific research.

Corrugated cardboard is an ecological material, mainly because, in addition to virgin cellulose fibers also the fibers recovered during recycling process are used in its production. However, the use of recycled fibers causes slight deterioration of the mechanical properties of the corrugated board. In addition, converting processes such as printing, die-cutting, lamination, etc. cause micro-damage in the corrugated cardboard layers. In this work, the focus is precisely on the crushing of corrugated cardboard. A series of laboratory experiments were conducted, in which the different types of single-walled corrugated cardboards were pressed in a fully controlled manner to check the impact of the crush on the basic material parameters. The amount of crushing (with a precision of 10 micrometers) was controlled by a precise FEMat device, for crushing the corrugated board in the range from 10 to 70% of its original thickness. In this study, the influence of crushing on bending, twisting and shear stiffness as well as a residual thickness and edge crush resistance of corrugated board was investigated. Then, a procedure based on a numerical homogenization, taking into account a partial delamination in the corrugated layers to determine the degraded material stiffness was proposed. Finally, using the empirical-numerical method, a simplified calculation model of corrugated cardboard was derived, which satisfactorily reflects the experimental results.

In the framework of this publication, silica-lignin hybrid materials were designed, obtained, characterized and then used as admixtures for cement composites. High-energy mechanical grinding of individual components was used to produce the systems that allowed ensuring adequate homogeneity of the final products. As a result of the analysis of Fourier transform infrared spectroscopy, it has been confirmed that weak physical interactions occur between the components. This allowed classifying the resulting systems as I class hybrid materials. In addition, the efficiency of obtaining final products was also inferred on the basis of obtained porous structure parameters and colorimetric data. The achieved bio-admixture with different weight ratios of silica and lignin was added to cement pastes in the amount ranging from 0.5 to 1 wt.%. The study showed that increasing the ratio of lignin in the admixture from 0.15 to 1 wt.% had a positive effect on the rheological properties of the pastes, while the mechanical properties of the composite were deteriorated. In turn, a higher amount of silica in the admixture acted in reverse. The most favorable results were obtained for a silica-lignin bio-admixture with a weight ratio of components equal to 5:1 wt./wt. A significant increase in compressive strength was gained at satisfactory plasticity of the paste.

The diamond-like carbon (DLC) coatings containing 1.6%, 5.3% and 9.4 at.% of Ti deposited by the radio frequency plasma enhanced chemical vapor deposition (RF PECVD) method on the silicon substrate were modified by n-decylphosphonic acid (DP) and 1H, 1H, 2H and 2H-perfluorodecylphosphonic acid (PFDP). The presence of perfluoro and alkylphosphonic self-assembled monolayers prepared by the liquid phase deposition (LPD) technique was confirmed by Fourier transform infrared spectroscopy (FTIR). It was shown that DP and PFDP monolayers on the surface of titanium incorporated diamond-like carbon (Ti-DLC) coatings had a huge influence on their wettability, friction properties, stability under phosphate- and tris-buffered saline solutions and on antimicrobial activity. It was also found that the dispersive component of surface free energy (SFE) had a significant influence on the value of the friction coefficient and the percentage value of the growth inhibition of bacteria. The dispersive component of SFE caused a reduction in the growth of bacteria and the friction coefficient in mili- and nano-newton load range. Additionally, both self-assembled monolayers prepared on Ti-DLC coatings strongly reduced bacterial activity by up to 95% compared to the control sample.

Apple pomace, a byproduct of juice production, is a rich source of bioactive compounds and nutrients. Supercritical fluid extraction was proposed as a method for a fast and selective extraction of hydrophobic compounds with a pharmaceutical potential from this matrix. Chromatographic analysis showed that the pomace contained significant amounts of such substances, the most abundant of them were ursolic acid, oleanolic acid, and β-sitosterol. The solubility was chosen as a primary factor for a selection of the extraction conditions; the best results were acquired for a temperature of 80 °C and a pressure of 30 MPa. The equation proposed by Chrastil was applied for the description of the impact of the process parameters on the solubility of the analytes; the obtained values of coefficients of determination were satisfactory, despite the fact that the equation was developed for binary systems. The extraction curves obtained during the experiments were used for the description of the process kinetics using the Broken plus Intact Cell model. The impact of the temperature, pressure, and flow rate of carbon dioxide on the mass transfer phenomena was investigated. The data obtained allowed the prediction of the extraction curve for the process conducted on the larger scale.

Mycotoxins are secondary fungal metabolites, toxic to humans, animals and plants. Under the influence of various factors, mycotoxins may undergo modifications of their chemical structure. One of the methods of mycotoxin modification is a transformation occurring in plant cells or under the influence of fungal enzymes. This paper reviews the current knowledge on the natural occurrence of the most important trichothecenes and zearalenone in cereals/cereal products, their metabolism, and the potential toxicity of the metabolites. Only very limited data are available for the majority of the identified mycotoxins. Most studies concern biologically modified trichothecenes, mainly deoxynivalenol-3-glucoside, which is less toxic than its parent compound (deoxynivalenol). It is resistant to the digestion processes within the gastrointestinal tract and is not absorbed by the intestinal epithelium; however, it may be hydrolysed to free deoxynivalenol or deepoxy-deoxynivalenol by the intestinal microflora. Only one zearalenone derivative, zearalenone-14-glucoside, has been extensively studied. It appears to be more reactive than deoxynivalenol-3-glucoside. It may be readily hydrolysed to free zearalenone, and the carbonyl group in its molecule may be easily reduced to α/β-zearalenol and/or other unspecified metabolites. Other derivatives of deoxynivalenol and zearalenone are poorly characterised. Moreover, other derivatives such as glycosides of T-2 and HT-2 toxins have only recently been investigated; thus, the data related to their toxicological profile and occurrence are sporadic. The topics described in this study are crucial to ensure food and feed safety, which will be assisted by the provision of widespread access to such studies and obtained results.

The presence of mycotoxins in cereal grain is a very important food safety factor. The occurrence of “masked” mycotoxins has been intensively investigated in recent years. In this study, the occurrence of nivalenol, deoxynivalenol-3-glucoside, and deoxynivalenol in 92 samples of winter wheat from Polish cultivars was determined. The frequency of the occurrence of deoxynivalenol and nivalenol in the samples was 83% and 70%, respectively. The average content of the analytes was: for deoxynivalenol 140.2 µg/kg (10.5–1265.4 µg/kg), for nivalenol 35.0 µg/kg (5.1–372.5 µg/kg). Deoxynivalenol-3-glucoside, the formation of which is connected with the biotransformation pathway in plants, was present in 27% of tested wheat samples; its average content was 41.9 µg/kg (15.8–137.5 µg/kg). The relative content of deoxynivalenol-3-glucoside (DON-3G) compared to deoxynivalenol (DON) in positive samples was 4–37%. Despite the high frequency of occurrence of these mycotoxins, the quality of wheat from the 2016 season was good. The maximum content of DON, as defined in EU regulations (1250 µg/kg), was exceeded in only one sample. Nevertheless, the presence of a glycosidic derivative of deoxynivalenol can increase the risk to food safety, as it can be hydrolyzed by intestinal microflora.

This paper is a study of the stability of fumonisins in dough based on maize flour prepared in a phosphate buffer with a pH of 3.5, 5.5 or 7.5 and baked at a temperature within the range of 100–250 °C. Buffers with various pH values were tested, since it is well-known that pH may significantly influence interactions of fumonisins with other substances. A standard analytical procedure was used to determine the concentration of free fumonisins. Hydrolysis in an alkaline medium was then applied to reveal the hidden forms, while the total fumonisins concentations was determined in another measurement. The total concentration of fumonisins was statistically higher in pH = 3.5 and pH = 5.5 than the concentration of free fumonisins; no similar difference was found at pH = 7.5. The applied phosphate buffer pH 7.5 may enhance solubility of fumonisins, which would increase extraction efficiency of free analytes, thereby decreasing the difference between concentrations of total and free fumonisins. Hydrolysed B1 fumonisin (HFB1) and partially hydrolysed B1 fumonisin (isomers a and b: PHFB1a and PHFB1b, respectively) were the main investigated substances. For baking temperatures below 220 °C, fumonisins were slightly more stable for pH = 5.5 than for pH = 3.5 and pH = 7.5. In both of these latter cases, the concentration of partially hydrolysed fumonisins grew initially (up to 200 °C) with an increase in the baking temperature, and then dropped. Similar behaviour was observed for free HFB1, which may suggest the following fumonisin degradation mechanism: initially, the tricarballylic acid (TCA) groups are removed from the molecules, and next, the HFB1 molecules disintegrate.

The levels of 26 mycotoxins were determined in 147 samples of the grain of cereals cultivated in five regions of Poland during the 2014 growing season. The HPLC-HRMS (time-of-flight) analytical technique was used. An analytical procedure to simultaneously determine 26 mycotoxins in grain was developed, tested and verified. Samples from eastern and southern Poland were more contaminated with mycotoxins than the samples from northern and western Poland. Toxins produced by Fusarium fungi were the main contaminants found. Some deoxynivalenol (DON) was found in 100% of the tested samples of wheat (Osiny, Borusowa, Werbkowice), triticale, winter barley and oats, while the maximum permissible DON level (as defined in the EU Commission Regulation No. 1881/2006) was exceeded in 10 samples. Zearalenone (ZEN), DON metabolites and enniatins were also commonly found. The presence of mycotoxins in grain reflected the prevailing weather conditions during the plant flowering/earing stages, which were favorable for the development of blight. Among all investigated wheat genotypes, cv. Fidelius was the least contaminated, while Bamberka, Forkida and Kampana were the most contaminated. However, the single-factor ANOVA analysis of variance did not reveal (at a statistical significance level α = 0.05) any differences between levels of mycotoxins in individual genotypes. Triticale was the most contaminated grain among all of the tested varieties. ZEN, DON and the sum of 3-acetyldexynivalenol and 15-acetyldeoxynivalenol (3- and 15-ADON) were found in 100% of the tested triticale samples at concentrations within the 4–86, 196–1326 and 36–374 µg·kg−1 range, respectively. Of particular concern was the fact that some “emerging mycotoxins” (enniatins) (in addition to commonly-known and legally-regulated mycotoxins) were also found in the tested triticale samples (enniatin B (Enn-B), enniatin B1 (Enn-B1), enniatin A-1 (Enn-A1), 100% of samples, and enniatin A (Enn-A), 70% of samples). Depending on the toxin, they were found at levels between 8 and 3328 µg·kg−1.

Introduction: Some evidence indicates that the improper trophoblast invasion of maternal spiral arteries could be caused by an imbalance between matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs), leading to preeclampsia (PE) development. This study aimed to assess the potential role of MMP1, MMP9, TIMP1 and TIMP2 gene polymorphisms in the pathogenesis of PE. Materials and methods: A total of 308 Polish women, 115 preeclamptic (55 with early-onset preeclampsia [EOPE], 60 with late-onset preeclampsia [LOPE]) and 193 healthy pregnant women, all of Caucasian origin, were recruited to the study. PE was diagnosed following the ACOG criteria. The polymorphic variants of the MMP-TIMP pathway (MMP1 rs1799750, MMP9 rs17576, MMP9 rs17577, TIMP1 rs4898, TIMP2 rs2277698, TIMP2 rs55743137) were genotyped by polymerase chain reaction and restriction fragment length polymorphism. Results: Analyzing all SNPs in the MMP-TIMP pathway, no significant differences in allele frequencies between preeclamptic women and controls were observed. However, comparing the EOPE and LOPE groups with each other, we observed a statistically significant difference between them for the TIMP1 rs4898 variant. In the whole group of 308 women, the mean placenta weight was the lowest in carriers of the rs4898 CC genotype. Post hoc analysis revealed significant differences between CC-CT (p = 0.0209) and CC-TT (p = 0.0469). Additionally, during allele analysis, a statistically significant difference in the mean placenta weight (for C allele 529.32 ± 157.11 g, for T allele 560.24 ± 162.24 g, p = 0.021) was also observed. Conclusion: Our findings suggest a relationship between TIMP1 rs4898 (372T > C) polymorphism and increased risk of early-onset preeclampsia in a population of pregnant Polish women. Our data suggest that the TIMP1 rs4898 C allele might be associated with increased risk for early-onset, but not for late-onset preeclampsia. To evaluate the role of the TIMP1 polymorphic variants in the etiopathology of preeclampsia, further studies with a larger sample size are needed.

(1) Background: there is a steady increase in the number of procedures performed via minimally invasive surgery, which have many benefits, but post-operative nausea and vomiting (PONV) and significant pain are still a common problem (2) Methods: 300 infertile women (18-40 years old) undergoing minimal invasive surgery. Interventions: laparoscopy and hysteroscopy performing, evaluation of postoperative symptoms, serotonin concentrations assessment, identify genetic polymorphisms. (3) Results: serotonin concentrations were significantly lower among women who required opioids ( p = 0.006). The presence of the GG genotype in the rs6318 polymorphism of the 5HTR2C gene had a protective effect on PONV (OR = 0.503; C.I. = [0.300-0.841]; p = 0.008), when the GG variant of the rs11214763 polymorphism of the 5HTR3B gene, when the risk of PONV was 1.65-fold higher (OR = 1.652; C.I. = [1.003-2.723]; p = 0.048). Pain intensity was significantly higher among women with GG genotype of the rs6296 polymorphism of the 5HTR1B gene (OR = 1.660; C.I. = [1.052-2.622]; p = 0.029).; (4) Conclusions: the evaluation of serotonin concentration predicts requirement for opioid pain relief medication. The polymorphisms of the serotonin receptors affect the intensity of postoperative complaints.