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The distribution range of the jumbo squid (Dosidicus gigas) has increased over the past few decades; it is now found in the entire Eastern Pacific Ocean and is considered an important species in the small and large fisheries of Chile, Perú, Ecuador, Costa Rica and Mexico but not in the Pacific region of Colombia. Three night-fishing campaigns were conducted over a year in the coastal Baudó-San Juan environmental unit, adjacent to the Bajo Baudó Encanto de Manglares Regional Integrated Management District in Chocó, at three locations 20 km, 35 km, and 45 km from the coastline to establish the availability and viability of jumbo squid as an alternative to the target species of artisanal fisheries in the Colombian Pacific. Night fishing sets were conducted at 60 m and 200 m depth at each sampling station to assess some of the biological parameters of the jumbo squid population (mantle length-ML, weight, growth and size at sexual maturity). Most catches were obtained in February and March. Growth was isometric, the average catch size was 24.3 cm ML, size at sexual maturity (L50) was 23.25 cm ML, estimated maximum size (L∞) was 39.9 cm ML, the growth constant (K) was 0.83, and mortality due to experimental fishing was 26 cm ML. According to our results, the jumbo squid fishery in the Colombian Pacific artisanal fishery would be viable during the first months of the year.

Human immunodeficiency virus-type 1 (HIV-1) remains one of the leading contributors to the global burden of disease, and novel antiretroviral agents with alternative mechanisms are needed to cure this infection. Here, we describe an exploratory attempt to optimize the antiretroviral properties of benfluron, a cytostatic agent previously reported to exhibit strong anti-HIV activity likely based on inhibitory actions on virus transcription and Rev-mediated viral RNA export. After obtaining six analogs designed to modify the benzo[c]fluorenone system of the parent molecule, we examined their antiretroviral and toxicity properties together with their capacity to recognize the Rev Recognition Element (RRE) of the virus RNA and inhibit the RRE–Rev interaction. The results indicated that both the benzo[c] and cyclopentanone components of benfluron are required for strong RRE–Rev target engagement and antiretroviral activity and revealed the relative impact of these moieties on RRE affinity, RRE–Rev inhibition, antiviral action and cellular toxicity. These data provide insights into the biological properties of the benzo[c]fluorenone scaffold and contribute to facilitating the design of new anti-HIV agents based on the inhibition of Rev function.

Antiviral agents are needed for the treatment of SARS-CoV-2 infections and to control other coronavirus outbreaks that may occur in the future. Here we report the identification and characterization of RNA-binding compounds that inhibit SARS-CoV-2 replication. The compounds were detected by screening a small library of antiviral compounds previously shown to bind HIV-1 or HCV RNA elements with a live-virus cellular assay detecting inhibition of SARS-CoV-2 replication. These experiments allowed detection of eight compounds with promising anti-SARS-CoV-2 activity in the sub-micromolar to micromolar range and wide selectivity indexes. Examination of the mechanism of action of three selected hit compounds excluded action on the entry or egress stages of the virus replication cycle and confirmed recognition by two of the molecules of conserved RNA elements of the SARS-CoV-2 genome, including the highly conserved S2m hairpin located in the 3’-untranslated region of the virus. While further studies are needed to clarify the mechanism of action responsible for antiviral activity, these results facilitate the discovery of RNA-targeted antivirals and provide new chemical scaffolds for developing therapeutic agents against coronaviruses.

In hospitals, operating rooms are energy-intensive spaces, due to the high flow of outside air required to achieve the necessary indoor air quality. Operating rooms demand ventilation continuously, despite periods of low daily surgical activity. However, by controlling ventilation during inactive periods in the operating room, significant energy savings can be achieved, avoiding penalties on IAQ levels. This paper evaluates the energy savings achieved by introducing ventilation flow control and regulation systems in operating rooms of hospitals located in different climatic zones within Spain. In addition, emissions and economic savings have also been evaluated. Two control and regulation strategies of the air flow to be supplied and extracted in the operating rooms, during periods of inactivity, are studied and include regulation by schedule and regulation by occupancy. Data from a 900-bed university hospital center are used as a reference to evaluate the average occupancy of the operating rooms, and the energy consumption, thus validating the model for calculating the demand of a typical operating room. The energy savings for the regulation by occupancy are 37.5%, and the regulation by schedule are 40% of the annual demand, with respect to an operating room working permanently.

New RNA-binding small-molecule scaffolds are needed to unleash the pharmacological potential of RNA targets. Here we have applied a pharmacophore-based virtual screening approach, seldom used in the RNA recognition field, to identify novel conformational inhibitors of the hepatitis C virus internal ribosome entry site. The conformational effect of the screening hits was assessed with a fluorescence resonance energy transfer assay, and the affinity, specificity, and binding site of the ligands were determined using a combination of fluorescence intensity and NMR spectroscopy experiments. The results indicate that this strategy can be successfully applied to discover RNA conformational inhibitors bearing substantially less positive charge than the reference ligands. This methodology can potentially be accommodated to other RNA motifs of pharmacological interest, facilitating the discovery of novel RNA-targeted molecules.

The purpose of this study was to identify clinical, analytical, and sociodemographic variables associated with the need for hospital admission in people over 50 years infected with SARS-CoV-2 and to assess whether diabetes mellitus conditions the risk of hospitalization. A multicenter case-control study analyzing electronic medical records in patients with COVID-19 from 1 March 2020 to 30 April 2021 was conducted. We included 790 patients: 295 cases admitted to the hospital and 495 controls. Under half ( n = 386, 48.8%) were women, and 8.5% were active smokers. The main comorbidities were hypertension (50.5%), dyslipidemia, obesity, and diabetes (37.5%). Multivariable logistic regression showed that hospital admission was associated with age above 65 years (OR from 2.45 to 3.89, ascending with age group); male sex (OR 2.15, 95% CI 1.47-3.15), fever (OR 4.31, 95% CI 2.87-6.47), cough (OR 1.89, 95% CI 1.28-2.80), asthenia/malaise (OR 2.04, 95% CI 1.38-3.03), dyspnea (4.69, 95% CI 3.00-7.33), confusion (OR 8.87, 95% CI 1.68-46.78), and a history of hypertension (OR 1.61, 95% CI 1.08-2.41) or immunosuppression (OR 4.97, 95% CI 1.45-17.09). Diabetes was not associated with increased risk of hospital admission (OR 1.18, 95% CI 0.80-1.72; p = 0.38). Diabetes did not increase the risk of hospital admission in people over 50 years old, but advanced age, male sex, fever, cough, asthenia, dyspnea/confusion, and hypertension or immunosuppression did.