Search

Your search keyword '"Lee, Hana"' showing total 26 results
Start Over Author "Lee, Hana" Publisher mdpi ag
26 results on '"Lee, Hana"'

3. Isolation and Expression of Transcription Factors Involved in Somatic Embryo Development by Transcriptome Analysis of Embryogenic Callus of Thuja koraiensis

Author

Ahn, Chang Ho, primary, Han, Jung Yeon, additional, Park, Hyeong Soo, additional, Yoon, Hyun Won, additional, Shin, Jung Won, additional, Seo, Jeong Min, additional, Lee, Hana, additional, Kim, Yeoung Ryul, additional, Baek, Saeng Geul, additional, Nam, Jae Ik, additional, Kim, Jung Min, additional, and Choi, Yong Eui, additional

Published
2023
Full Text
View/download PDF

25. Comparison of the Antioxidant Potency of Four Triterpenes of Centella asiatica against Oxidative Stress.

Author

Lim J, Lee H, Hong S, Lee J, and Kim Y

Abstract

We comparatively evaluated the antioxidant properties of key triterpenes from Centella asiatica , including asiatic acid (AA), asiaticoside, madecassic acid, and madecassoside, in several cell types, including skin fibroblasts, macrophages, hepatocytes, and endothelial cells, under conditions promoting oxidative stress. AA conferred the highest viability on Hs68 cells exposed to ultraviolet B (UVB) irradiation. Triterpene pretreatment attenuated the UVB-induced generation of reactive oxygen species (ROS) and malondialdehyde (MDA), as well as the UVB-induced depletion of glutathione (GSH) in skin fibroblasts. AA most potently inhibited UVB-induced MMP generation, resulting in increased intracellular collagen levels. Pretreatment with triterpenes, particularly AA, significantly improved cell viability and attenuated TBHP-induced levels of ROS, alanine aminotransferase, and aspartate aminotransferase in HepG2 cells. Triterpenes attenuated ROS levels and reduced MDA and GSH expression in EA.hy926 cells. In RAW264.7 macrophages, production of nitric oxide, tumor necrosis factor-α, and interleukin-6 (indicators of LPS-induced oxidative damage) was significantly reduced by treatment with any of the triterpenes. Statistical analyses of triterpene biological activities using principal component analysis and hierarchical clustering revealed that AA exerted the greatest overall influence and showed remarkable activity in Hs68 and HepG2 cells.

Published
2024
Full Text
View/download PDF

26. Micro-Current Stimulation Can Modulate the Adipogenesis Process by Regulating the Insulin Signaling Pathway in 3T3-L1 Cells and ob / ob Mice.

Author

Lee H, Lee JH, Kim D, Hwang D, Lee M, Chung H, Kim TJ, and Kim HS

Abstract

Obesity is a disease in which fat is abnormally or excessively accumulated in the body, and many studies have been conducted to overcome it with various techniques. In this study, we evaluated whether micro-current stimulation (MCS) can be applied to prevent obesity by regulating the adipogenesis through 3T3-L1 cells and ob / ob mice. To specify the intensity of MCS, Oil Red O staining was conducted with various intensities of MCS. Based on these, subsequent experiments used 200 and 400 μA for the intensity of MCS. The expressions of insulin signaling pathway-related proteins, including phosphorylation of IGF-1 and IR, were decreased in all MCS groups, and in turn, downstream signals such as Akt and ERK were decreased. In addition, MCS reduced the nucleus translocation of PPAR-γ and decreased the protein expression of C/EBP-α. In the ob / ob mouse model, MCS reduced body weight gain and abdominal adipose tissue volume. In particular, the concentration of triglycerides in serum was also decreased. Taken together, our findings showed that MCS inhibited lipid accumulation by regulating insulin signaling in 3T3-L1, and it was effective at reducing body weight and adipose tissue volume in ob / ob mice. These suggest that MCS may be a useful treatment approach for obesity.

Published
2023
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results