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Your search keyword '"Li, Lingyu"' showing total 31 results
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31 results on '"Li, Lingyu"'

22. Fucoidan from Laminaria japonica Inhibits Expression of GLUT9 and URAT1 via PI3K/Akt, JNK and NF-κB Pathways in Uric Acid-Exposed HK-2 Cells

Author

Jiang-Hua Shang, Zheng Haiying, Xiaohui Tan, Huazhong Liu, Yang Chunyan, Zhen Lin, Fangping Li, Li Lingyu, and Yu Zhang

Subjects
0301 basic medicine, animal structures, Organic Cation Transport Proteins, QH301-705.5, Glucose Transport Proteins, Facilitative, Organic Anion Transporters, Pharmaceutical Science, glucose transporter 9, Article, Cell Line, Gout Suppressants, Kidney Tubules, Proximal, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, fucoidan, uric acid, Polysaccharides, Drug Discovery, medicine, Humans, Hyperuricemia, Biology (General), Pharmacology, Toxicology and Pharmaceutics (miscellaneous), Protein kinase B, PI3K/AKT/mTOR pathway, Fucoidan, Reabsorption, urate transporter 1, JNK Mitogen-Activated Protein Kinases, NF-kappa B, Glucose transporter, NF-κB, medicine.disease, Cell biology, 030104 developmental biology, chemistry, 030220 oncology & carcinogenesis, Uric acid, Laminaria, Phosphatidylinositol 3-Kinase, Proto-Oncogene Proteins c-akt, Signal Transduction
Abstract

This work aimed to investigate the effect of fucoidan (FPS) on urate transporters induced by uric acid (UA). The results showed that UA stimulated the expression of glucose transporter 9 (GLUT9) and urate transporter 1 (URAT1) in HK-2 cells, and FPS could reverse the effect. Moreover, UA could activate NF-κB, JNK and PI3K/Akt pathways, but both pathway inhibitors and FPS inhibited the UA-induced activation of these three pathways. These data suggested that FPS effectively inhibited the expression induction of reabsorption transporters URAT1 and GLUT9 by UA, through repressing the activation of NF-κB, JNK and PI3K/Akt signal pathways in HK-2 cells. The in vitro research findings support the in vivo results that FPS reduces serum uric acid content in hyperuricemia mice and rats through inhibiting the expression of URAT1 and GLUT9 in renal tubular epithelial cells. This study provides a theoretical basis for the application of FPS in the treatment of hyperuricemia.

Published
2021

30. DNA Barcoding Unveils Novel Discoveries in Authenticating High-Value Snow Lotus Seed Food Products.

Author

Zhao G, Li L, Shen X, Zhong R, Zhong Q, and Lei H

Abstract

Snow Lotus Seed (SLS), esteemed for its nutritional and market value, faces challenges of authentication due to the absence of appropriate testing standards and methods. This results in frequent adulteration of SLS sourced from Gleditsia sinensis ( G. sinensis ) with other plant seeds endosperm. Traditional chloroplast DNA barcoding methods are inadequate for species identification due to the absence of chloroplasts in G. sinensis seeds endosperm. In this study, the homology of 11 ITS genes among 6 common Gleditsia species was analyzed. Universal primers suitable for these species were designed and screened. A DNA barcoding method for distinguishing SLS species was developed using Sanger sequencing technology, leveraging existing GenBank and Barcode of Life Data System (BOLD) databases. Optimized sample pretreatment facilitated effective DNA extraction from phytopolysaccharide-rich SLS. Through testing of commercial SLS products, the species origin has been successfully identified. Additionally, a novel instance of food fraud was uncovered, where the Caesalpinia spinosa endosperm was used to counterfeit SLS for the first time. The study established that the developed DNA barcoding method is effective for authenticating SLS species. It is of great significance for combating food fraud related to SLS, ensuring food safety, and promoting the healthy development of the SLS industry.

Published
2024
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31. Variations in VOCs Emissions and Their O 3 and SOA Formation Potential among Different Ages of Plant Foliage.

Author

Zhang B, Qiao L, Han H, Xie W, and Li L

Abstract

Volatile organic compounds (VOCs) emitted by plant foliage play an important role in ozone (O 3 ) and secondary organic aerosol (SOA) formation. Their emissions can be influenced by the leaf age. We explored the VOCs emissions and their effects on the formation of O 3 and SOA from plant foliage in different ages. VOCs emissions from the young, mature, and senescent leaves of Ginkgo biloba , Ligustrum lucidum , and Forsythia suspensa were measured using the dynamic enclosure system and the TD-GC-MS technique. Based on the emission rates of quantified compounds, their potential to form O 3 and SOA was estimated. Results showed that there were significant differences in the VOCs emission rate and their composition among leaves in different ages. The emission rate of the total VOCs by young leaves was the highest, while the lowest by senescent leaves. Monoterpenes were the dominant VOCs category, and isoprene emission had the lowest contribution for the leaves at each age. With increasing leaf age, the proportion of monoterpenes emission increased, and the proportion of sesquiterpenes decreased. The variations of isoprene and other VOCs were different. The potentials of total VOCs, isoprene, monoterpenes, sesquiterpenes, and other VOCs to form O 3 (OFP) and SOA (SOAP) varied significantly among leaves at different ages. The total OFP and SOAP were the highest by young leaves, while the lowest by senescent leaves. With increasing leaf age, the contribution of monoterpenes to OFP and SOAP also increased, while that of sesquiterpenes decreased. Our study will provide support for the more accurate parameterization of the emission model and help to understand the VOCs emissions and study the precise prevention and control of complex air pollution at different times.

Published
2023
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