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25 results on '"Naas, Thierry"'

5. Evaluation of InTray Cassettes Directly from Blood Cultures for the Diagnosis of Sepsis in Clinical Bacteriology Laboratories as an Alternative to Classic Culture Media

Author

Natale, Alessandra, primary, Oueslati, Saoussen, additional, Rochard, Alice, additional, Ombelet, Sien, additional, Lopez-Baez, Daniel, additional, Hardy, Liselotte, additional, Cunningham, Jane, additional, Franquesa, Céline, additional, Vandenberg, Olivier, additional, Ronat, Jean-Baptiste, additional, and Naas, Thierry, additional

Published
2023
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7. Clinical Added Value of SARS-CoV-2 Antigen Detection in Blood Samples

Author

Oueslati, Saoussen, primary, Manai Bouokazi, Melek, additional, Ramdhani, Ikrame, additional, Escaut, Lélia, additional, Pham, Tài, additional, Ouzani, Souad, additional, Anguel, Nadia, additional, Bulifon, Sophie, additional, Vauloup-Fellous, Christelle, additional, Coilly, Audrey, additional, Legros, Laurence, additional, Guichardon, Magali, additional, Fortineau, Nicolas, additional, Dortet, Laurent, additional, Roque-Afonso, Anne-Marie, additional, and Naas, Thierry, additional

Published
2022
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9. Prevalence and Molecular Mechanisms of Carbapenem Resistance among Gram-Negative Bacilli in Three Hospitals of Northern Lebanon

Author

Rima, Mariam, primary, Oueslati, Saoussen, additional, Dabos, Laura, additional, Daaboul, Dina, additional, Mallat, Hassan, additional, Bou Raad, Elie, additional, Achkar, Marcel, additional, Mawlawi, Osman, additional, Bernabeu, Sandrine, additional, Bonnin, Rémy A., additional, Girlich, Delphine, additional, Osman, Marwan, additional, Hamze, Monzer, additional, and Naas, Thierry, additional

Published
2022
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12. Computational Modeling and Design of New Inhibitors of Carbapenemases: A Discussion from the EPIC Alliance Network

Author

Dahdouh, Elias, primary, Allander, Lisa, additional, Falgenhauer, Linda, additional, Iorga, Bogdan I., additional, Lorenzetti, Stefano, additional, Marcos-Alcalde, Íñigo, additional, Martin, Nathaniel I., additional, Martínez-Martínez, Luis, additional, Mingorance, Jesús, additional, Naas, Thierry, additional, Rubin, Joseph E., additional, Spyrakis, Francesca, additional, Tängdén, Thomas, additional, and Gómez-Puertas, Paulino, additional

Published
2022
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18. Rapid Detection of VanA/B-Producing Vancomycin-Resistant Enterococci Using Lateral Flow Immunoassay

Author

Oueslati, Saoussen, primary, Gonzalez, Camille, additional, Volland, Hervé, additional, Cattoir, Vincent, additional, Bernabeu, Sandrine, additional, Girlich, Delphine, additional, Dulac, Duncan, additional, Plaisance, Marc, additional, Boutigny, Laure, additional, Dortet, Laurent, additional, Simon, Stéphanie, additional, and Naas, Thierry, additional

Published
2021
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21. A Lateral Flow Immunoassay for the Rapid Identification of CTX-M-Producing Enterobacterales from Culture Plates and Positive Blood Cultures

Author

Bernabeu, Sandrine, primary, Ratnam, Kayaththiry Caroline, additional, Boutal, Hervé, additional, Gonzalez, Camille, additional, Vogel, Anaïs, additional, Devilliers, Karine, additional, Plaisance, Marc, additional, Oueslati, Saoussen, additional, Malhotra-Kumar, Surbhi, additional, Dortet, Laurent, additional, Fortineau, Nicolas, additional, Simon, Stéphanie, additional, Volland, Hervé, additional, and Naas, Thierry, additional

Published
2020
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24. Molecular, Genetic, and Biochemical Characterization of OXA-484 Carbapenemase, a Difficult-to-Detect R214G Variant of OXA-181.

Author

Gonzalez C, Oueslati S, Rima M, Nermont R, Dortet L, Hopkins KL, Iorga BI, Bonnin RA, and Naas T

Abstract

OXA-244, an R214G variant of OXA-48, is silently spreading worldwide likely because of difficulties in detection using classical screening media. Here, we characterized two clinical isolates of Escherichia coli and Citrobacter youngae that displayed reduced susceptibility to carbapenems but were lacking significant carbapenemase activity as revealed by negative Carba NP test results. However, positive test results were seen for OXA-48-like enzymes by lateral flow immunoassays. WGS revealed the presence of a bla OXA-181-like gene that codes for OXA-484, an R214G variant of OXA-181. Bla OXA-484 gene was located on a 58.4-kb IncP1-like plasmid (pN-OXA-484), that upon transfer into E. coli HB4 with impaired permeability, conferred carbapenem and temocillin resistance (MICs > 32 mg/L). E. coli TOP10 (pTOPO-OXA-484) revealed reduced MICs in most substrates as compared to E. coli TOP10 (pTOPO-OXA-181), especially for imipenem (0.25 mg/L versus 0.75 mg/L) and temocillin (16 mg/L versus 1028 mg/L). Catalytic efficiencies of OXA-484 were reduced as compared to OXA-181 for most ß-lactams including imipenem and temocillin with 27.5- and 21.7-fold reduction, respectively. Molecular modeling confirmed that the salt bridges between R214, D159, and the R1 substituent's carboxylate group of temocillin were not possible with G214 in OXA-484, explaining the reduced affinity for temocillin. In addition, changes in active site's water network may explain the decrease in hydrolysis rate of carbapenems. OXA-484 has weak imipenem and temocillin hydrolytic activities, which may lead to silent spread due to underdetection using selective screening media or biochemical imipenem hydrolysis confirmatory tests.

Published
2024
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25. Validation of Three MicroScan ® Antimicrobial Susceptibility Testing Plates Designed for Low-Resource Settings.

Author

Ronat JB, Oueslati S, Natale A, Kesteman T, Elamin W, Langendorf C, Hardy L, Vandenberg O, and Naas T

Abstract

Easy and robust antimicrobial susceptibility testing (AST) methods are essential in clinical bacteriology laboratories (CBL) in low-resource settings (LRS). We evaluated the Beckman Coulter MicroScan lyophilized broth microdilution panel designed to support Médecins Sans Frontières (MSF) CBL activity in difficult settings, in particular with the Mini-Lab. We evaluated the custom-designed MSF MicroScan Gram-pos microplate (MICPOS1) for Staphylococcus and Enterococcus species, MSF MicroScan Gram-neg microplate (MICNEG1) for Gram-negative bacilli, and MSF MicroScan Fastidious microplate (MICFAST1) for Streptococci and Haemophilus species using 387 isolates from routine CBLs from LRS against the reference methods. Results showed that, for all selected antibiotics on the three panels, the proportion of the category agreement was above 90% and the proportion of major and very major errors was below 3%, as per ISO standards. The use of the Prompt inoculation system was found to increase the MIC and the major error rate for some antibiotics when testing Staphylococci. The readability of the manufacturer's user manual was considered challenging for low-skilled staff. The inoculations and readings of the panels were estimated as easy to use. In conclusion, the three MSF MicroScan MIC panels performed well against clinical isolates from LRS and provided a convenient, robust, and standardized AST method for use in CBL in LRS.

Published
2022
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