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We evaluated the effects of fungicides on strawberry powdery mildew (PM) fungus, Podosphaera aphanis, using an electrostatic technique. Thirty-six fungicides were sprayed on single colonies of P. aphanis on leaves of strawberry seedlings (Fragaria × ananassa Duchesne ex Rozier). Colony development varied depending on the tested fungicides. Particularly, pyraziflumid, triflumizole, triforine, polyoxin, sodium hydrogen carbonate + copper wettable powder, and flutianil + mepanipyrim were highly effective for reducing colony development. P. aphanis colonies were histochemically stained to observe the morphological characteristics of fungal cells forming normal and abnormal conidiophores. Abnormal conidiophores were classified into seven types based on their morphological and cytological characteristics. Finally, asexual conidia were collected from single P. aphanis colonies on the leaves spray-treated with fungicides using a dielectrically polarized insulator plate (electrostatic spore collector); conidia attracted to the insulator plates were counted using a high-fidelity digital microscope. Most tested fungicides highly inhibited the production and/or germination of asexual conidia. The germination of asexual conidia was observed only in thiophanate-methyl (methyl benzimidazole carbamates fungicides; MBC fungicides) and azoxystrobin (quinone outside inhibitors; QoI fungicides). Assessing with the electrostatic technique, we clarified that P. aphanis has developed resistance to both thiophanate-methyl and azoxystrobin. Thus, the methodological assessment analyzing the colony development and the number of conidia released from single colonies will be helpful information for screening effective fungicides.

Dielectric barrier discharge plasma actuators (DBD-PAs) have the potential to improve the performance of fluid machineries such as aircrafts and wind turbines by preventing flow separation. In this study, to identify the multiple flow control mechanisms in high Reynolds number flow, parametric experiments for an actuation parameter F+ with a wide range of Re values (105–106) for NACA0015 airfoil was conducted. We conducted wind tunnel tests by applying a DBD-PA to the flow field around a wing model at the leading edge. Lift characteristics, turbulent kinetic energy in the flow field, shear layer height, and the separation point of the boundary layer were evaluated based on pressure distributions on the wing surface and velocity of the flow field, with the effect of DBD-PA on the post-stall flow around the wing and the mechanism behind the increase in the lift coefficient CL analyzed based on these evaluation results. The following phenomena contributed to the increase in CL: (1) increase in turbulent kinetic energy; (2) increase in circulation; and (3) acceleration of the flow near the leading edge. Thus, this study effectively investigated the dependence of the increase in lift on F+ and the lift-increasing mechanism for a wide range of Re values.

Data assimilation using particle image velocimetry (PIV) and Reynolds-averaged Navier–Stokes (RANS) simulation was performed for an ideally expanded supersonic jet flying at a Mach number of 2.0. The present study aims to efficiently reconstruct all the physical quantities in the aeroacoustic fields that match well with a realistic, experimentally obtained flow field. The two-dimensional, two-component PIV measurement was applied to the jet axis plane, and the time-averaged velocity field was obtained using single-pixel ensemble correlation. Two-dimensional axisymmetric RANS simulation using the Menter shear stress transport (SST) model was also performed, and the parameters of the SST model were optimized via data assimilation using the ensemble Kalman filter. The standard deviation of the observation noise σ, which is a parameter of the ensemble Kalman filter, is estimated by the previously proposed method (Nakamura et al., Low-Grid-Resolution-RANS-Based Data Assimilation of Time-Averaged Separated Flow Obtained by LES. Int. J. Comp. Fluid. Dyn., 2022), and its effectiveness was investigated for the first time. This method effectively estimated the magnitude of σ at each generation without tuning the hyperparameters. The assimilated flow fields exhibited similar flow structures observed in PIV such as the potential core length or shear layer. Therefore, the present framework can be used to estimate time-averaged full flow fields that match well with experimentally observed flow fields, and has the potential to construct a database for the Navier-Stokes-based stability analysis that requires a full flow field.

For many years, we have been developing flexible sensors made of braided piezoelectric poly-l-lactic acid (PLLA) fibers that can be tied and untied for practical applications in society. To ensure good quality of sleep, the occurrence of bruxism has been attracting attention in recent years. Currently, there is a need for a system that can easily and accurately measure the frequency of bruxism at home. Therefore, taking advantage of the braided piezoelectric PLLA cord sensor’s unique characteristic of being sewable, we aimed to provide a system that can measure the frequency of bruxism using the braided piezoelectric PLLA cord sensor simply sewn onto a bed sheet on which the subject lies down. After many tests using trial and error, the sheet sensor was completed with zigzag stitching. Twenty subjects slept overnight in a hospital room on sheets integrated with a braided piezoelectric PLLA cord. Polysomnography (PSG) was simultaneously performed on these subjects. The results showed that their bruxism could be detected with an accuracy of more than 95% compared with PSG measurements, which can only be performed in a hospital by a physician and are more burdensome for the subjects, with the subjects simply lying on the bed sheet with a braided piezoelectric PLLA cord sensor sewn into it.

Trophoblast cell surface antigen 2 (Trop-2, encoded by TACSTD2) is the target protein of sacituzumab govitecan, a novel antibody-drug conjugate for locally advanced or metastatic urothelial carcinoma. However, the expression status of Trop-2 in upper tract urothelial carcinoma (UTUC) remains unclear. We performed immunohistochemical analysis of 99 UTUC samples to evaluate the expression status of Trop-2 in patients with UTUC and analyze its association with clinical outcomes. Trop-2 was positive in 94 of the 99 UTUC samples, and high Trop-2 expression was associated with favorable progression-free survival (PFS) and cancer-specific survival (p = 0.0011, 0.0046). Multivariate analysis identified high Trop-2 expression as an independent predictor of favorable PFS (all cases, p = 0.045; high-risk group (pT3≤ or presence of lymphovascular invasion or lymph node metastasis), p = 0.014). Gene expression analysis using RNA sequencing data from 72 UTUC samples demonstrated the association between high TACSTD2 expression and favorable PFS (all cases, p = 0.069; high-risk group, p = 0.029). In conclusion, we demonstrated that Trop-2 is widely expressed in UTUC. Although high Trop-2 expression was a favorable prognostic factor in UTUC, its widespread expression suggests that sacituzumab govitecan may be effective for a wide range of UTUC.

This study numerically investigated the mechanisms of separation control using a synthetic jet (SJ) and plasma actuator (PA) around an NACA0015 airfoil at the chord Reynolds number of 63,000. Both SJ and PA were installed on the leading edge with the same order of input momentum (Cμ=O(10−3–10−5)) and the same actuation frequencies in F+=1.0–30. The momentum coefficient Cμ is defined as the normalized momentum introduced from the SJ or the PA, and F+ stands for the actuation frequency normalized by the chord length and uniform velocity. A number of large-eddy simulations (LES) were conducted for the SJ and the PA, and the mechanisms were clarified in terms of the exchange of chordwise momentum with Reynolds shear stress and coherent vortex structures. First, four main differences in the induced flows of the SJ and the PA were clarified as follows: (A) wall-tangential velocity; (B) three-dimensional flow structures; (C) spatial locality; and (D) temporal fluctuation. Then, a common feature of flow control by the SJ and the PA was revealed: a lift-to-drag ratio was found to be better recovered in F+=6.0–20 than in other frequencies. Although there were differences in the induced flows, the phase decomposition of the flow fields identified common mechanisms that the turbulent component of the Reynolds shear stress mainly contributes to the exchange of the chordwise (streamwise) momentum; and the turbulent vortices are convected over the airfoil surface by the coherent spanwise vortices in the frequency of F+.

“Handshaking parties”, where pop idols shake hands with fans, can be exciting. The multimodal stimulation of tactile, visual, and auditory sensations can be captivating. In this study, we presented subjects with stimuli eliciting three sensory responses: tactile, visual, and auditory sensations. We found that the attraction scores of subjects increased because they felt the smoothness and obtained a human-like sensory experience grasping a grip handle covered with artificial skin, faux fur, and abrasive cloth with their dominant hand as they looked at a picture of a pop idol or listened to a song. When no pictures or songs were presented, a simple feeling of slight warmth was correlated with the attraction score. Results suggest that multimodal stimuli alter tactile sensations and the feelings evoked. This finding may be useful for designing materials that activate the human mind through tactile sensation and for developing humanoid robots and virtual reality systems.

Among the mycoparasites, Ampelomyces strains are studied in detail, particularly regarding their use as biocontrol agents (BCAs) of powdery mildew (PM) fungi, including their potential to replace conventional agrochemicals. Ampelomyces strains are characterized morphologically; their ribosomal DNA internal transcribed spacer (rDNA-ITS) regions and actin gene (ACT) fragments were sequenced and their mycoparasitic activity was analyzed. In the interaction between Ampelomyces strains and PM fungi, the spores of the mycoparasites germinate on plant leaves, and their hyphae then penetrate the hyphae of PM fungi. Ampelomyces hyphae continue their growth internally, initiating the atrophy of PM conidiophores and eventually their complete collapse. Following the successful destruction of PM hyphae by Ampelomyces, the mycoparasite produces new intracellular pycnidia in PM conidiophores. The progeny spores released by mature pycnidia become the sources of subsequent infections of intact PM hyphae. As a result, the number of Ampelomyces-inoculated PM colonies gradually declines, and the conidial release of PM colonies is inhibited after the first treatment. Almost all conidiophores of 5- and 10-day-old Ampelomyces-inoculated PM colonies undergo complete atrophy or collapse. Methodological advances and in-depth analyses of the Ampelomyces–PM interaction were recently published. In this review, we summarize the genetic and phylogenetic diversity, the timing of mycoparasitism and pycnidiogenesis, the results of quantitative and visual analyses using electrostatic and digital microscopy technologies, the PM biocontrol potential of Ampelomyces, and the potential commercialization of the mycoparasites. The information provided herein can support further biocontrol and ecological studies of Ampelomyces mycoparasites.

Optimization approaches that determine sensitive sensor nodes in a large-scale, linear time-invariant, and discrete-time dynamical system are examined under the assumption of independent and identically distributed measurement noise. This study offers two novel selection algorithms, namely an approximate convex relaxation method with the Newton method and a gradient greedy method, and confirms the performance of the selection methods, including a convex relaxation method with semidefinite programming (SDP) and a pure greedy optimization method proposed in the previous studies. The matrix determinant of the observability Gramian was employed for the evaluations of the sensor subsets, while its gradient and Hessian were derived for the proposed methods. In the demonstration using numerical and real-world examples, the proposed approximate greedy method showed superiority in the run time when the sensor numbers were roughly the same as the dimensions of the latent system. The relaxation method with SDP is confirmed to be the most reasonable approach for a system with randomly generated matrices of higher dimensions. However, the degradation of the optimization results was also confirmed in the case of real-world datasets, while the pure greedy selection obtained the most stable optimization results.

This paper presents the experimental results of separation and reattachment transient flow processes over a NACA0015 airfoil wing when using a plasma actuator for flow control. In addition, it addresses the flow behavior in the transient processes when the flow control device is activated or deactivated, providing insights for future feedback-based active flow control. This approach offers the benefit of enhanced aerodynamic capabilities. The experiments were conducted at a Reynolds number of 66,000 and an angle of attack of 13 degrees for leading-edge separation without control. The plasma actuator was installed on the leading edge of the wing, with a voltage of 8 kV, base frequency of 30 kHz, and burst frequencies ranging from 100 Hz to 600 Hz. Particle image velocimetry was employed for the flow field velocity measurements, and surface pressure data were obtained using eight piezoelectric pressure sensors. The first proper orthogonal decomposition mode of the transient flow velocity field is the focus of this paper and the flow behavior is quantitatively discussed. The results reveal details about the flow separation and reattachment transient processes such as their flow structures and their evolution over time. It is concluded that the time asymmetry between the separation and reattachment transient processes could be leveraged for further improvements to the efficiency of actuators.

Timely progression of the meiotic cell cycle and synchronized establishment of male meiosis in anthers are key to ascertaining plant fertility. With the discovery of novel regulators of the plant cell cycle, the mechanisms underlying meiosis initiation and progression appear to be more complex than previously thought, requiring the conjunctive action of cyclins, cyclin-dependent kinases, transcription factors, protein–protein interactions, and several signaling components. Broadly, cell cycle regulators can be classified into two categories in plants based on the nature of their mutational effects: (1) those that completely arrest cell cycle progression; and (2) those that affect the timing (delay or accelerate) or synchrony of cell cycle progression but somehow complete the division process. Especially the latter effects reflect evasion or obstruction of major steps in the meiosis but have sometimes been overlooked due to their subtle phenotypes. In addition to meiotic regulators, very few signaling compounds have been discovered in plants to date. In this review, we discuss the current state of knowledge about genetic mechanisms to enter the meiotic processes, referred to as the mitosis-meiosis fate decision, as well as the importance of callose (β-1,3 glucan), which has been unsung for a long time in male meiosis in plants.

In this study, we evaluated the effectiveness of hyperparasitic fungi in controlling powdery mildew (PM). In a greenhouse, we spray-inoculated single colonies of the melon PM-causing fungus Podosphaera xanthii strain KMP-6N at three different fungal developmental stages (i.e., 5, 10, and 15 days old) with spores of the hyperparasitic fungus Ampelomyces sp. strain Xs-q. After spray inoculation, we collected and counted KMP-6N conidia produced as asexual progeny from PM colonies using an electrostatic rotational spore collector. Collector insulator films were replaced at 24 h intervals until KMP-6N ceased to release additional progeny conidia. Conidial releases from each of the single Xs-q-inoculated KMP-6N colonies gradually reduced, then stopped within ca. 4 and 8 days of the first treatment in 5- and 10-day-old KMP-6N colonies, and within ca. 20 days of the second spray treatment in 15-day-old KMP-6N colonies, respectively. The total numbers of asexual progeny conidia collected from single 5-, 10-, and 15-day-old colonies were ca. 156, 1167, and 44,866, respectively. After electrostatic spore collection, conidiophores in Xs-q-uninoculated KMP-6N colonies appeared normal, whereas almost all conidiophores in 5- and 10-day-old Xs-q-inoculated KMP-6N colonies were completely deformed or collapsed due to the infection of the hyperparasitic fungus. This is the first study to apply electrostatic and digital microscopic techniques to clarify the impact of fungal hyperparasitism on mycohost survival, and, in particular, to assess quantitatively and visually the suppression of conidial release from any PM colonies infected with Ampelomyces.

In this study, an experimental investigation of separation control using a dielectric barrier discharge plasma actuator was performed on an NACA0015 airfoil over a wide range of Reynolds numbers, angles of attack, and nondimensional burst frequencies. The range of the Reynolds number was based on a chord length ranging from 2.52 × 105 to 1.008 × 106. A plasma actuator was installed at the leading edge and driven by AC voltage. Burst mode (duty-cycle) actuation was applied, with the nondimensional burst frequency ranging between 0.1–30. The control authority was evaluated using the time-averaged distribution of the pressure coefficient Cp and the calculated value of the lift coefficient Cl. The baseline flow fields were classified into three types: (1) leading-edge separation; (2) trailing-edge separation; and (3) the hysteresis between (1) and (2). The results of the actuated cases show that the control trends clearly depend on the differences in the separation conditions. In leading-edge separation, actuation with a burst frequency of approximately F+= 0.5 creates a wide negative pressure region on the suction-side surface, leading to an increase in the lift coefficient. In trailing-edge separation, several actuations alter the position of turbulent separation.

Powdery mildew fungi produce progeny conidia on conidiophores, and promote the spread of powdery mildew diseases by dispersal of the conidia from conidiophores in the natural environment. To gain insights and devise strategies for preventing the spread of powdery mildew infection, it is important to clarify the ecological mechanism of conidial dispersal from conidiophores. In this study, all of the progeny conidia released from single colonies of strawberry powdery mildew fungus (Podosphaera aphanis (Wallroth) U. Braun and S. Takamatsu var. aphanis KSP-7N) on true leaves of living strawberry plants (Fragaria × ananassa Duchesne ex Rozier cv. Sagahonoka) were consecutively collected over the lifetime of the colony with an electrostatic rotational spore collector (insulator drum) under greenhouse conditions, and counted under a high-fidelity digital microscope. The insulator drum consisted of a round plastic container, copper film, thin and transparent collector film, electrostatic voltage generator, and timer mechanism. When negative charge was supplied from the voltage generator to the copper film, the collector film created an attractive force to trap conidia. The electrostatically activated collector film successfully attracted progeny conidia released from the colony. Experiment was carried out at just one colony on one leaf for each month (in February, May, July, October, November, and December in 2021), respectively. Each collector film was exchanged for a new collector film at 24 h intervals until KSP-7N ceased to release progeny conidia from single colonies. Collection experiments were carried out to estimate the total number of conidia released from a single KSP-7N colony over a 35–45-day period after inoculation. During the fungal lifetime, KSP-7N released an average of 6.7 × 104 conidia from each of the single colonies at approximately 816 h. In addition, conidial release from KSP-7N colonies was largely affected by the light intensity and day length throughout a year; the number of conidia released from single KSP-7N colonies in night-time was clearly smaller than that in daytime, and the time of conidial release from single KSP-7N colonies was shorter by approximately 2 to 4 h in autumn and winter than in spring and summer. The ecological characteristics related to conidial releases from KSP-7N colonies will be helpful information for us to successfully suppress the spread of strawberry powdery mildews onto host plants under greenhouse conditions.

In this study, the stabilities and conversion efficiencies of perovskite solar cells including cesium (Cs) or formamidinium (FA) at the CH₃NH₃ site were investigated. The additive effects on the photovoltaic properties and crystalline structures were investigated via current–voltage measurements, X-ray diffraction, and scanning electron microscopy. The simultaneous co-addition of Cs and FA to the CH3NH3PbI3 perovskite crystal improved the photovoltaic properties, which may be due to the suppression of the decomposition of the perovskite crystals and the promotion of crystal growth.

Antioxidants were applied to a dual-luminophore pressure-sensitive paint (PSP), and the effects on photodegradation caused by exposure to excitation light were studied. Three types of antioxidants that are commonly used for the photostability enhancement of polymers were added to a dual-luminophore PSP, and degradation rates and pressure/temperature sensitivities were investigated by coupon-based tests. One-hour-long aging tests were performed in a pressure chamber with a continuous excitation light source under dry air and argon atmospheres at 100 kPa and 20 °C. As a result of the aging tests, a singlet oxygen quencher type antioxidant was found to reduce the degradation rate by 91% when compared with the dual-luminophore PSP without antioxidants. This implies that singlet oxygen has a dominant role in the photodegradation mechanism of the dual-luminophore PSP.

This review examines the progress of electrostatic spore-trapping research and the potential for the practical application of electrostatic apparatuses in powdery mildew control. These apparatuses produce an electric field by charging an insulated conductor wire (ICW). Airborne pathogen spores are subjected to an attractive force in the electric field and are drawn to the charged ICW as a result of dielectrophoretic movement. The strength of the attractive force is commensurate with the field strength (determined by the magnitude of the voltage applied to the ICW). Single-charged monopolar electric field screens (SM screens) are constructed by arraying negatively charged cylindrical ICWs in parallel at a specific interval. The connected electric fields of these ICWs form a gap-free air-shielding barrier. Wind-dispersed spores are precipitated by this barrier to create spore-free air. Oppositely charged SM screens have been combined to develop double-charged dipolar electric field screens, which generate a stronger spore attraction force under lower voltage application. Thus, electric field screens represent a promising physical method for creating spore-free spaces in cropping facilities, where plants can be cultivated without risk of infection by airborne fungal pathogens.

An unattended pest control system was developed to eliminate whiteflies (Bemisia tabaci) that settled on greenhouse tomato plants. The system exploited the whitefly’s habit of flying up from a plant that was mechanically tapped and then heading toward yellow objects. Remote-controlled dollies with arms that tapped plants and yellow-colored double-charged dipolar electric field screens (YDD-EFSs) (oppositely electrified transparent insulator tubes filled with yellow-colored water) attracted and trapped the whiteflies. The whiteflies flew up when the plants were mechanically tapped with the dolly’s arms during reciprocating movements and were subsequently trapped by YDD-EFSs that were automatically translocated to the target plants. The system was applied to rows of whitefly-infested tomato plants. Almost all whiteflies transferred to plants were successfully recovered by two dollies moving on either side of the plants, approaching all plants individually (via programmed movement). In summary, we present an efficient unattended method for controlling whiteflies on tomato plants in greenhouses.

We developed and evaluated an anodized-aluminum pressure-sensitive paint (AA-PSP) with new formulations of free-base porphyrin, H2TCPP, as an optical unsteady pressure sensor. The luminophore H2TCPP has quite a short fluorescent lifetime (2.4 ns on the condition of the AA-PSP). The fluorescence spectroscopy result shows that the excitation wavelength of H2TCPP corresponds to violet-colored (425 nm) and green-colored (longer than 520 nm) lights. The pressure sensitivity is sufficiently high for the pressure sensor (0.33–0.51%/kPa) and the temperature sensitivity is very low (0.07–1.46%/K). The photodegradation of the AA-PSPs is not severe in both excitation light sources of the green LED and the Nd:YAG laser. The resonance tube experiment result shows the cut-off frequency of the AA-PSPs is over 9.0 kHz, and the results of the shock tube experiment show the 10 µs order time constant of the normal shock wave.

Electrostatic devices generating an electric field (EF) are promising tools for greenhouse tomato cultivation. In these devices, an EF is generated in the space surrounding an insulated conductor (IC) that is charged by a voltage generator. Thus, a physical force is exerted on any insect that enters the EF, as a negatively charged IC (NC-IC) pushes a negative charge (free electrons) out of the insect body. The insect is polarized positively to be attracted to the NC-IC, and a grounded metal net (G-MN) repels the insect. This dual function of the apparatus (insect capture and repulsion) is the core of the electrostatic pest-exclusion strategy. In this study, we applied various innovative EF-based devices to evaluate their efficacy in greenhouse tomato cultivation. Our objective was to determine the optimal apparatus for simple, inexpensive construction by greenhouse workers. The results of this study will contribute to the development of sustainable pest-management protocols in greenhouse horticulture.

Ovarian cancer has the highest mortality rate among all gynecological malignancies; therefore, a novel treatment strategy is needed urgently. Utilizing immune checkpoint inhibitors has been considered for microsatellite instability (MSI)-high (MSI-H) tumors. However, the prevalence of MSI-H tumors in ovarian endometrioid and clear cell carcinomas remains unclear. Here, polymerase chain reaction was used to analyze 91 cases of ovarian endometrioid and clear cell carcinomas for the MSI status and the relationship between MSI-H, immune checkpoint molecules, and clinicopathological factors (including patient survival). Only 5 of 91 (5%) cases were MSI-H endometrioid carcinomas. In these cases, CD-8 expression was significantly higher (p = 0.026), confirming an enhanced immune response. From the survival curve, no statistical correlations were found between the MSI-H group and the microsatellite stable (MSS) group; however, the MSS group trended towards better progression-free survival than the MSI-H group (p = 0.056). Patients with PD-L1 expression had shorter overall survival than those without (p = 0.022). Thus, MSI-H is a rare event and not a favorable prognostic factor in ovarian endometrioid and clear cell carcinomas. Thus, to improve the prognosis of ovarian endometrioid carcinoma and clear cell carcinomas, a combination therapy of immune checkpoint inhibitors and other molecular targeted therapies may be required.

In the present study, we analyzed negative electricity released from insects captured by an electric field (EF)-producing apparatus. Adult houseflies (Musca domestica) were used as the model insect. The EF producer consisted of a negatively charged polyvinyl chloride membrane-insulated iron plate (N-PIP) and a non-insulated grounded iron plate (GIP) paralleled with the N-PIP. An EF was formed in the space between the plates. A housefly placed on the GIP was physically attracted to the N-PIP, and electricity released from the fly was detected as a specific transient electric current at the time of attraction and during subsequent confinement of the fly to the N-PIP. The magnitude of the insect-derived electric current became larger as the voltage applied to the N-PIP increased. We determined the total amount of electric current and confinement time within the apparatus necessary to kill all captured flies. These results demonstrate the insecticidal function and insect-capturing ability of the EF-producing apparatus.

Type I trichomes of tomato leaves (Solanum lycopersicum Mill. cv. Moneymaker), as outgrowths of the plant epidermis, are suitable for monitoring infection processes of powdery mildew species using a high-fidelity digital microscope (DM) without fungal staining. On the trichomes, tomato powdery mildew (Pseudoidium neolycopersici L. Kiss) isolate KTP-03 produced a maximum of four vigorously elongated hyphae per conidium, which stopped growth approximately 12 days after inoculation. Single trichome cells, invaded by fungal hyphae at various fungal infection stages during the 12-day period after the inoculation of single conidia, were cut at the bases and directly collected with small precision scissors (i.e., microscissors) held by the manipulator under a DM. Subsequently, suc-polymerase chain reaction (PCR) (reverse transcription (RT)-PCR followed by nested (N)-PCR) was conducted to explore gene expression in the infected trichome. We selected intron-containing genes from tomatoes and powdery mildew fungi for the detection of constitutive gene transcripts, namely plasma membrane H+-ATPase (LHA2) and β-tubulin 2 (TUB2) genes. In suc-PCR, a single band from spliced mRNAs of both LHA2 and TUB2 genes were detected, suggesting that both genes were successfully transcribed in single KTP-03-infected trichomes. With combined primers for both LHA2 and TUB2 (multiplex RT-PCR/N-PCR), two bands were detected through the amplification of intron-spliced mRNAs of both genes. Therefore, our single-trichome cell PCR amplification method is effective for detecting the expression patterns of genes from both tomato and powdery mildew fungus. Combinations of digital microscopy, microscissors, and multiplex RT-PCR/N-PCR amplification techniques will be useful for simultaneously analysing the molecular interactions between plants and powdery mildew fungi at the level of single tomato leaf trichome cells. Also, this employed technique will be of benefit in other plant species and crops, possessing leaf trichome cells, to elucidate the molecular interactions between plants and pathogens.

The development of floral organs is coordinated by an elaborate network of homeotic genes, and gibberellin (GA) signaling is involved in floral organ development; however, the underlying molecular mechanisms remain elusive. In the present study, we found that MOS4-ASSOCIATED COMPLEX 5A (MAC5A), which is a protein containing an RNA-binding motif, was involved in the development of sepals, petals, and stamens; either the loss or gain of MAC5A function resulted in stamen malformation and a reduced seed set. The exogenous application of GA considerably exacerbated the defects in mac5a null mutants, including fewer stamens and male sterility. MAC5A was predominantly expressed in pollen grains and stamens, and overexpression of MAC5A affected the expression of homeotic genes such as APETALA1 (AP1), AP2, and AGAMOUS (AG). MAC5A may interact with RABBIT EARS (RBE), a repressor of AG expression in Arabidopsis flowers. The petal defect in rbe null mutants was at least partly rescued in mac5a rbe double mutants. These findings suggest that MAC5A is a novel factor that is required for the normal development of stamens and depends on the GA signaling pathway.

Powdery mildew fungi infect plant leaves, reducing the yield of infected melon plants. Therefore, an eco-friendly method of controlling powdery mildew in melon plants needs to be developed. A previous study described how the morphological characteristics of the conidiophores of the melon powdery mildew fungus Podosphaera xanthii Pollacci (designated KMP-6N) grown under greenhouse (natural) conditions and red light-emitting diode (LED) irradiation differed from those grown under growth chamber conditions and blue LED irradiation. In the present study, conidiophores with unconstricted conidia under blue light were collected and inoculated onto host leaves through micromanipulation; the unconstricted conidia germinated and infected the leaves, producing vigorously elongated hyphae. The number of conidia collected, the initial times of conidial release from single colonies, and the number of conidia remaining in chains on conidiophores were examined with electrostatic techniques. Under red light, the number of collected conidia gradually increased with the light irradiation period. The initial conidial release occurred between 2 to 4 h; the number of conidia remaining on the conidiophores gradually decreased and, eventually, the conidiophore lengths became shorter. In contrast, under blue light, few conidia were collected at any given time; the number of conidia on the conidiophores gradually increased and, eventually, the conidiophore lengths became longer. Next, the effects of red and blue light on the spread of powdery mildew infection by placing a KMP-6N-infected melon seedling at the centre of a tray containing healthy melon seedlings were examined. Almost all healthy seedlings caused powdery mildew symptoms at ca. 21 days after red light irradiation, whereas only healthy seedlings near the infected seedlings showed symptoms after blue light irradiation. Thus, the spread of melon powdery mildew infection clearly differed between red and blue light irradiation. This is the first report describing the effects of red and blue light on the spread of P. xanthii infection from a single infected seedling to healthy host seedlings; their results provide insight into the ecological mechanisms of powdery mildew conidial scatter from conidiophores.

We propose a markerless image alignment method for pressure-sensitive paint measurement data replacing the time-consuming conventional alignment method in which the black markers are placed on the model and are detected manually. In the proposed method, feature points are detected by a boundary detection method, in which the PSP boundary is detected using the Moore-Neighbor tracing algorithm. The performance of the proposed method is compared with the conventional method based on black markers, the difference of Gaussian (DoG) detector, and the Hessian corner detector. The results by the proposed method and the DoG detector are equivalent to each other. On the other hand, the performances of the image alignment using the black marker and the Hessian corner detector are slightly worse compared with the DoG and the proposed method. The computational cost of the proposed method is half of that of the DoG method. The proposed method is a promising for the image alignment in the PSP application in the viewpoint of the alignment precision and computational cost.

Fucosylation is an oligosaccharide modification that plays an important role in immune response and malignancy, and specific fucosyltransferases (FUTs) catalyze the three types of fucosylations: core-type, Lewis type, and H type. FUTs regulate cancer proliferation, invasiveness, and resistance to chemotherapy by modifying the glycosylation of signaling receptors. Oligosaccharides on PD-1/PD-L1 proteins are specifically fucosylated, leading to functional modifications. Expression of FUTs is upregulated in renal cell carcinoma, bladder cancer, and prostate cancer. Aberrant fucosylation in prostate-specific antigen (PSA) could be used as a novel biomarker for prostate cancer. Furthermore, elucidation of the biological function of fucosylation could result in the development of novel therapeutic targets. Further studies are needed in the field of fucosylation glycobiology in urological malignancies.

Our aim was to develop an electrostatic apparatus to lure and capture silverleaf whiteflies (Bemisia tabaci), vegetable leafminers (Liriomyza sativae), and western flower thrips (Frankliniella occidentalis) that invade tomato greenhouses. A double-charged dipolar electric field producer (DD-EFP) was constructed by filling water in two identical transparent soft polyvinyl chloride tubes arrayed in parallel with fixed separation, and then, inserting the probes of grounded negative and positive voltage generators into the water of the two tubes to generate negatively and positively charged waters, respectively. These charged waters electrified the outer surfaces of the opposite tubes via dielectric polarization. An electric field formed between the oppositely charged tubes. To lure these phototactic insects, the water was colored yellow using watercolor paste, then introduced into the transparent insulator tubes to construct the yellow-colored DD-EFP. This apparatus lured insects in a manner similar to commercially available yellow sticky traps. The yellow-colored DD-EFP was easily placed as a movable upright screen along the plants, such that invading pests were preferentially attracted to the trap before reaching the plants. Furthermore, pests settling on the plants were attracted to the apparatus, which used a plant-tapping method to drive them off the plants. Our study provided an experimental basis for developing an electrostatic device to attract and capture insects that enter greenhouses.

We proposed a new prototype sensor system to understand the workload of employees during telework. The goal of sensing using such a system is to index the degree of stress experienced by employees during work and recognize how to improve their work environment. Currently, to realize this, image processing technology with a Web camera is generally used for vital sign sensing. However, it creates a sense of discomfort at work because of a strong sense of surveillance. To truly evaluate a working environment, it is necessary that an employee be unaware of the sensor system and for the system to be as unobtrusive as possible. To overcome these practical barriers, we have developed a new removable piezoelectric sensor incorporated in a piezoelectric poly-L-lactic acid (PLLA) braided cord. This cord is soft and flexible, and it does not cause any discomfort when attached to the cushion cover sheet. Thus, it was possible to measure the workload of an employee working from home without the employee being aware of the presence of a sensor. Additionally, we developed a system for storing data in a cloud system. We succeeded in acquiring continuous long-term data on the vital signs of employees during telework using this system. The analysis of the data revealed a strong correlation between behavior and stress.

This study analysed the mechanism of avoidance behaviour by adult Turkestan cockroaches (Shelfordella lateralis Walker) in response to a static electric field (S-EF) formed in the space between a negatively charged polyvinyl chloride-insulated iron plate (N-PIP) and a grounded metal net (G-MN). The negative surface charge supplied to the iron plate by a voltage generator caused the G-MN to polarise positively via electrostatic induction. In the S-EF, the negative charge of the N-PIP created a repulsive force that pushed free electrons in the field toward the ground via the G-MN. When insects released in the space surrounded by the S-EF inserted their antennae into the S-EF, they pulled them back reflexively and moved backward. The analysis indicated that an electric current flowed transiently toward the ground when an insect inserted its antennae into the S-EF. The insect became positively charged via this discharge and was attracted to the opposite pole (N-PIP). In response to this attractive force, the insect pulled its antennae back quickly. The positive electrification caused by the removal of free electrons from the antenna tip triggered the avoidance behaviour.

In this study, the propagation time and attenuation rate distributions of each sound source grid point (200 × 200) to a microphone of an arbitrary position across the shear layer, which are required for beamforming, were reconstructed by the reduced-order model with sparse sampling for acceleration of the computation. First, the propagation time and attenuation rate distributions, including the refraction of sound by the shear layer were calculated over 100 patterns of combinations of the wind speed and the microphone position, as training data. The dominant modes and optimum sampling points were discovered from the training data. Subsequently, data-driven sparse sampling for reconstruction was applied and the propagation time and the attenuation rate from each grid point (200 × 200) to a microphone were quickly calculated for the given microphone position and wind speed. The error of the obtained calculation result is 1% or less, and the approximation by data-driven sparse sampling is concluded to be effective.

The characteristics of fast-response pressure-sensitive paints (PSPs) in low-pressure conditions were evaluated. Three representative porous binders were investigated: polymer-ceramic PSP (PC-PSP), anodized-aluminum PSP (AA-PSP), and thin-layer chromatography PSP (TLC-PSP). For each PSP, two types of luminophores, Pt(II) meso-tetra (pentafluorophenyl) porphine (PtTFPP) and tris(bathophenanthroline) ruthenium dichloride (Ru(dpp)3), were used as sensor molecules. Pressure sensitivities, temperature sensitivities, and photodegradation rates were measured and evaluated using a pressure chamber. The effect of ambient pressure on the frequency response was investigated using an acoustic resonance tube. The diffusivity coefficients of PSPs were estimated from the measured frequency response and luminescent lifetime, and the governing factor of the frequency response under low-pressure conditions was identified. The results of static calibration show that PC-PSP/PtTFPP, AA-PSP/Ru(dpp)3, and TLC-PSP/PtTFPP have high pressure sensitivities that exceed 4%/kPa under low-pressure conditions and that temperature sensitivity and photodegradation rates become lower as the ambient pressure decreases. Dynamic calibration results show that the dynamic characteristics of PSPs with PtTFPP are dependent on the ambient pressure, whereas those of PSPs with Ru(dpp)3 are not influenced by the ambient pressure. This observation indicates that the governing factor in the frequency response under low-pressure conditions is the lifetime for PC-PSP and TLC-PSP, whereas the governing factor for AA-PSP is diffusion.

Catastrophic earthquake-induced landslides can occur on slopes composed of loosened and fractured rock masses. Although it is impossible to prevent such landslides, estimation of the susceptibility of slopes to earthquake-induced landslides is useful for risk management. An index of susceptibility to earthquake-induced landslides (ISEL) was developed by using helicopter-borne electromagnetic resistivity data. However, the ISEL has not yet been validated through the analysis of pre-earthquake data. In this study, ISEL values were estimated from resistivity and digital elevation data obtained in 2013 around Mt. Aso, Kyushu, before the 2016 Kumamoto earthquake. Although most of the landslides around Mt. Aso during the 2016 Kumamoto earthquake were mass movements of volcanic tephra layers, some of them occurred on loosened rock masses. Landslide susceptible areas at loosened rock masses are the target for ISEL value estimation. Our results validated the effectiveness of the ISEL as a predictor of earthquake-induced rock mass landslides.

The purpose of the study was to construct an electrostatic insect-capturing apparatus that could be applied to a drone (quadcopter). For this purpose, a double-charged dipolar electric field screen (DD-screen) was constructed using oppositely charged insulator tubes that was then attached to a drone. For charging, the inner surface of the tubes was coated with a conductive paste and then linked to a negative or positive voltage generator. The opposite charges of the tubes formed an electric field between them and created an attractive force to capture insects that entered the field. The DD-screen constructed here was sufficiently light to enable its attachment to a drone. The screen was hung from the drone perpendicular to the direction of drone movement, so as to receive the longitudinal airflow produced by the movement of the drone. It was positioned 1.8 m below the drone body to avoid the influence of the downward slipstream generated by the rotating propellers. Eventually, the drone was able to conduct a stable flight, with sufficient endurance, and captured airborne insects carried by an airflow of 8 m/s during the flight. This study, therefore, provides an experimental basis for establishing a new method for conducting trap-based monitoring of airborne insects during remote-controlled flight through operation of a DD-screen attached to a drone.

The effects of the oxygen mole fraction on the static properties of pressure-sensitive paint (PSP) were investigated. Sample coupon tests using a calibration chamber were conducted for poly(hexafluoroisopropyl methacrylate)-based PSP (PHFIPM-PSP), polymer/ceramic PSP (PC-PSP), and anodized aluminum PSP (AA-PSP). The oxygen mole fraction was set to 0.1–100%, and the ambient pressure (Pref) was set to 0.5–140 kPa. Localized Stern–Volmer coefficient Blocal increased and then decreased with increasing oxygen mole fraction. Although Blocal depends on both ambient pressure and the oxygen mole fraction, its effect can be characterized as a function of the partial pressure of oxygen. For AA-PSP and PHFIPM-PSP, which are low-pressure- and relatively low-pressure-type PSPs, respectively, Blocal peaks at PO2ref<12 kPa. In contrast, for PC-PSP, which is an atmospheric-pressure-type PSP in the investigated range, Blocal does not have a peak. Blocal has a peak at a relatively high partial pressure of oxygen due to the oxygen permeability of the polymer used in the binder. The peak of SPR, which is the emission intensity change with respect to normalized pressure fluctuation, appears at a lower partial pressure of oxygen than that of Blocal. This is because the intensity of PSP becomes quite low at a high partial pressure of oxygen even if Blocal is high. Hence, the optimal oxygen mole fraction depends on the type of PSP and the ambient pressure range of the experiment. This optimal value can be found on the basis of the partial pressure of oxygen.

In the present study, an electrostatic apparatus for trapping adult tomato leaf miner flies (Liriomyza sativae) emerging from underground pupae at the surface of a seedbed in an organic greenhouse was developed. The apparatus consisted of insulated iron rods arranged in parallel at set intervals and linked to a voltage generator, which supplied a negative charge to the rods, as well as non-insulated grounded iron rods with the same configuration. The two layers of insulated and non-insulated iron rods were arrayed in parallel to form a static electric field between the layers. The electric field created a strong attractive force capable of capturing flies that entered the field. In a greenhouse assay, the apparatus was placed horizontally above a seedbed in a greenhouse and surveyed for its ability to capture adult flies emerging from pupae that were introduced onto the seedbed beneath the apparatus. The results revealed that the apparatus effectively trapped all adult flies that emerged from the pupae and that it functioned stably while continuously operated during the entire period of the experiment. Thus, our novel apparatus is a promising tool for the physical control of adult tomato leaf miners in the insecticide-independent cultivation of greenhouse tomatoes.

The invasive kudzu vine Pueraria montana var. lobata is an agricultural nuisance that disturbs the field cultivation of crop plants. We developed a simple electrostatic method of suppressing the invasive growth of kudzu vines as an alternative to the use of herbicides for weed control. Exposure of the vine apex to a high-voltage arc discharge was the focal point of the study. To achieve this, we constructed a ladder-shaped apparatus by arranging several parallel copper rods at specific intervals in an insulating frame. The top rod was linked to a direct current voltage generator and pulse-charged at −10 kV, and the remaining rods were linked to a grounded line. Because of the conductive nature of the grounded vine body, the vine climbing along the grounded rods was subjected to a pulsed arc discharge from the charged rod when its apex entered the electric field produced around the charged rod. The part of the vine exposed to the discharge was heated, which promoted vaporisation of body water. This destroyed the tip growing point and prevented vine elongation. A simplified weed control apparatus was developed, which can be fabricated for practical use from inexpensive, ready-made materials.

We have established an inflammation-related carcinogenesis model in mouse, in which regressive QR-32 cells subcutaneously co-implanted with a foreign body—gelatin sponge—convert themselves into lethal tumors due to massive infiltration of inflammatory cells into the sponge. Animals were fed with a diet containing 5% or 10% fermented brown rice and rice bran with Aspergillus oryzae (FBRA). In 5% and 10% FBRA diet groups, tumor incidences were lower (35% and 20%, respectively) than in the non-treated group (70%). We found that FBRA reduced the number of inflammatory cells infiltrating into the sponge. FBRA administration did not cause myelosuppression, which indicated that the anti-inflammatory effects of FBRA took place at the inflammatory lesion. FBRA did not have antitumor effects on the implanted QRsP-11 tumor cells, which is a tumorigenic cell line established from a tumor arisen after co-implantation of QR-32 cells with sponge. FBRA did not reduce formation of 8-hydroxy-2′-deoxyguanine adducts, a marker of oxidative DNA damage in the inflammatory lesion; however, it reduced expression of inflammation-related genes such as TNF-α, Mac-1, CCL3 and CXCL2. These results suggest that FBRA will be an effective chemopreventive agent against inflammation-related carcinogenesis that acts by inhibiting inflammatory cell infiltration into inflammatory lesions.