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18 results on '"Olmos, Antonio"'

4. High-Throughput Sequencing Discloses the Cucumber Mosaic Virus (CMV) Diversity in Slovakia and Reveals New Hosts of CMV from the Papaveraceae Family

Author

Mrkvová, Michaela, primary, Hančinský, Richard, additional, Predajňa, Lukáš, additional, Alaxin, Peter, additional, Achs, Adam, additional, Tomašechová, Jana, additional, Šoltys, Katarína, additional, Mihálik, Daniel, additional, Olmos, Antonio, additional, Ruiz-García, Ana Belén, additional, and Glasa, Miroslav, additional

Published
2022
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13. High-Throughput Sequencing Reveals Further Diversity of Little Cherry Virus 1 with Implications for Diagnostics

Author

Katsiani, Asimina, primary, Maliogka, Varvara, additional, Katis, Nikolaos, additional, Svanella-Dumas, Laurence, additional, Olmos, Antonio, additional, Ruiz-García, Ana, additional, Marais, Armelle, additional, Faure, Chantal, additional, Theil, Sébastien, additional, Lotos, Leonidas, additional, and Candresse, Thierry, additional

Published
2018
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17. Olive Leaf Mottling Virus: A New Member of the Genus Olivavirus .

Author

Ruiz-García AB, Candresse T, Malagón J, Ruiz-Torres M, Paz S, Pérez-Sierra A, and Olmos A

Abstract

Studies of the virome of olive trees with symptoms of leaf mottling by high-throughput sequencing (HTS) revealed the presence of a new virus. Full coding genome sequences of two isolates were determined and consisted of a single RNA segment of 16,516 nt and 16,489, respectively. The genomic organization contained 10 open reading frames (ORFs) from 5' to 3': ORF1a, ORF1b (RdRp), ORF2 (p22), ORF3 (p7), ORF4 (HSP70h), ORF5 (HSP90h), ORF6 (CP), ORF7 (p19), ORF8 (p12), ORF9 (p23) and ORF10 (p9). Phylogenetic analyses clustered this virus in the genus Olivavirus , family Closteroviridae, with the closest species being Olivavirus flaviolae , commonly named olive leaf yellowing-associated virus (OLYaV). However, amino acid sequences of all taxonomically relevant proteins showed, in all cases, a divergence higher than 25% between OLYaV and the new virus, indicating that it represents a new species in the genus Olivavirus for which the common name of olive leaf mottling virus (OLMV) is proposed. This study represents an advance in the genus Olivavirus and provides new insights into the olive virome.

Published
2024
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18. Development and Validation of a Duplex RT-qPCR for Detection of Peach Latent Mosaic Viroid and Comparison of Different Nucleic-Acid-Extraction Protocols.

Author

Luigi M, Taglienti A, Corrado CL, Cardoni M, Botti S, Bissani R, Casati P, Passera A, Miotti N, De Jonghe K, Everaert E, Olmos A, Ruiz-García AB, and Faggioli F

Abstract

Peach latent mosaic viroid (PLMVd) is an important pathogen that causes disease in peaches. Control of this viroid remains problematic because most PLMVd variants are symptomless, and although there are many detection tests in use, the reliability of PCR-based methods is compromised by the complex, branched secondary RNA structure of the viroid and its genetic diversity. In this study, a duplex RT-qPCR method was developed and validated against two previously published single RT-qPCRs, which were potentially able to detect all known PLMVd variants when used in tandem. In addition, in order to simplify the sample preparation, rapid-extraction protocols based on the use of crude sap or tissue printing were compared with commercially available RNA purification kits. The performance of the new procedure was evaluated in a test performance study involving five participant laboratories. The new method, in combination with rapid-sample-preparation approaches, was demonstrated to be feasible and reliable, with the advantage of detecting all different PLMVd isolates/variants assayed in a single reaction, reducing costs for routine diagnosis.

Published
2023
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