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Your search keyword '"Priscila Emiko Kobayashi"' showing total 4 results
Start Over Author "Priscila Emiko Kobayashi" Publisher mdpi ag
4 results on '"Priscila Emiko Kobayashi"'

1. Effects of Lapatinib on HER2-Positive and HER2-Negative Canine Mammary Carcinoma Cells Cultured In Vitro

Author

Antonio Fernando Leis-Filho, Patrícia de Faria Lainetti, Priscila Emiko Kobayashi, Carlos Eduardo Fonseca-Alves, and Renée Laufer-Amorim

Subjects
dog, cancer, comparative oncology, molecular targets, Pharmacy and materia medica, RS1-441
Abstract

HER2 is a prognostic and predictive marker widely used in breast cancer. Lapatinib is a tyrosine kinase inhibitor that works by blocking the phosphorylation of the receptor HER2. Its use is related to relatively good results in the treatment of women with HER2+ breast cancer. Thus, this study aimed to verify the effects of lapatinib on four canine primary mammary gland carcinoma cell cultures and two paired metastatic cell cultures. Cultures were treated with lapatinib at concentrations of 100, 500, 1000 and 3000 nM for 24 h and the 50% inhibitory concentration (IC50) for each cell culture was determined. In addition, a transwell assay was performed to assess the ability of lapatinib to inhibit cell migration. Furthermore, we verified HER2 expression by RT-qPCR analysis of cell cultures and formalin-fixed paraffin-embedded tissues from samples corresponding to those used in cell culture. Lapatinib was able to inhibit cell proliferation in all cell cultures, but it was not able to inhibit migration in all cell cultures. The higher the expression of HER2 in a culture, the more sensitive the culture was to treatment. This relationship may be an indication that the expression of HER2 may be a predictive factor and opens a new perspective for the treatment of primary and metastatic mammary gland cancer.

Published
2021
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2. Characterization of Collagen Fibers (I, III, IV) and Elastin of Normal and Neoplastic Canine Prostatic Tissues

Author

Luis Gabriel Rivera Calderón, Priscila Emiko Kobayashi, Rosemeri Oliveira Vasconcelos, Carlos Eduardo Fonseca-Alves, and Renée Laufer-Amorim

Subjects
dog, prostatic tissue, extracellular matrix, picrosirius, immunohistochemistry, Veterinary medicine, SF600-1100
Abstract

This study aimed to investigate collagen (Coll-I, III, IV) and elastin in canine normal prostate and prostate cancer (PC) using Picrosirius red (PSR) and Immunohistochemical (IHC) analysis. Eight normal prostates and 10 PC from formalin-fixed, paraffin-embedded samples were used. Collagen fibers area was analyzed with ImageJ software. The distribution of Coll-I and Coll-III was approximately 80% around prostatic ducts and acini, 15% among smooth muscle, and 5% surrounding blood vessels, in both normal prostate and PC. There was a higher median area of Coll-III in PC when compared to normal prostatic tissue (p = 0.001 for PSR and p = 0.05 for IHC). Immunostaining for Coll-IV was observed in the basal membrane of prostate acini, smooth muscle, blood vessels, and nerve fibers of normal and PC samples. Although there was no difference in Coll-IV area between normal tissue and PC, tumors with Gleason score 10 showed absence of Coll-IV, when compared to scores 6 and 8 (p = 0.0095). Elastic fibers were found in the septa dividing the lobules and around the prostatic acini of normal samples and were statistically higher in PC compared to normal tissue (p = 0.00229). Investigation of ECM components brings new information and should be correlated with prognosis in future studies.

Published
2019
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3. Establishment and Characterization of Canine Mammary Gland Carcinoma Cell Lines with Vasculogenic Mimicry Ability in vitro and in vivo

Author

Andressa Brandi, Antonio Fernando Leis Filho, Renée Laufer-Amorim, Priscila Emiko Kobayashi, Maria Carolina Mangini Prado, Patrícia de Faria Lainetti, and Carlos Eduardo Fonseca-Alves

Subjects
medicine.anatomical_structure, In vivo, Cell culture, Mammary gland, Keratin 8, medicine, Cancer research, Vasculogenic mimicry, Biology, Cell bank, Phenotype, In vitro
Abstract

Mammary neoplasms affect a population of uncastrated and elderly female dogs and most of these neoplasms are malignant. In order to study this disease cell culture presents itself as a promising preclinical model, creating the opportunity to deposit cell lines at a cell bank, allowing a great repetition of the assays and making the validation of the results more reliable. Including, in vitro experiments for vasculogenic mimicry (VM) evaluation. VM is related to cancer cells capable of generate vascular-like structures without endothelial cells, mimicking the vasculogenic process. The aim of this study was to establish and characterize ten cell lines from canine mammary gland tumour according to immunophenotype and tumorigenicity, and with its ability to form vasculogenic mimicry-like structures in vitro. Fifteen samples from canine mammary gland carcinoma were collected and cultured in vitro and ten cell lines were established and characterized. Cells were evaluated for morphology, phenotype, vascular mimicry and tumorigenicity. All cell lines presented spindle shape morphology and expressed concomitant pan-cytokeratin and CK8/18. Four cell lines had vasculogenic mimicry ability and two cell showed in vivo tumorigenic potential. Cell characterization of those lines will help to create a database for more knowledge of mammary gland carcinomas in dogs, including studies of tumor behavior and new therapeutic targets.

Published
2019
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4. Characterization of Collagen Fibers (I, III, IV) and Elastin of Normal and Neoplastic Canine Prostatic Tissues

Author

Rosemeri de Oliveira Vasconcelos, Carlos Eduardo Fonseca-Alves, Luis Gabriel Rivera Calderón, Priscila Emiko Kobayashi, Renée Laufer-Amorim, and Universidade Estadual Paulista (Unesp)

Subjects
0301 basic medicine, Prostatic tissue, Pathology, medicine.medical_specialty, extracellular matrix, Article, Picrosirius red, Extracellular matrix, 03 medical and health sciences, Prostate cancer, 0302 clinical medicine, Prostate, Dog, medicine, oncology_oncogenics, lcsh:Veterinary medicine, General Veterinary, biology, Chemistry, picrosirius, medicine.disease, Immunohistochemistry, Picrosirius, 030104 developmental biology, medicine.anatomical_structure, 030220 oncology & carcinogenesis, dog, immunohistochemistry, biology.protein, lcsh:SF600-1100, Elastin, Immunostaining, prostatic tissue
Abstract

Made available in DSpace on 2019-10-06T16:41:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) This study aimed to investigate collagen (Coll-I, III, IV) and elastin in canine normal prostate and prostate cancer (PC) using Picrosirius red (PSR) and Immunohistochemical (IHC) analysis. Eight normal prostates and 10 PC from formalin-fixed, paraffin-embedded samples were used. Collagen fibers area was analyzed with ImageJ software. The distribution of Coll-I and Coll-III was approximately 80% around prostatic ducts and acini, 15% among smooth muscle, and 5% surrounding blood vessels, in both normal prostate and PC. There was a higher median area of Coll-III in PC when compared to normal prostatic tissue (p = 0.001 for PSR and p = 0.05 for IHC). Immunostaining for Coll-IV was observed in the basal membrane of prostate acini, smooth muscle, blood vessels, and nerve fibers of normal and PC samples. Although there was no difference in Coll-IV area between normal tissue and PC, tumors with Gleason score 10 showed absence of Coll-IV, when compared to scores 6 and 8 (p = 0.0095). Elastic fibers were found in the septa dividing the lobules and around the prostatic acini of normal samples and were statistically higher in PC compared to normal tissue (p = 0.00229). Investigation of ECM components brings new information and should be correlated with prognosis in future studies. Department of Veterinary Pathology School of Agricultural and Veterinarian Sciences São Paulo State University (Unesp) Department of Veterinary Clinic School of Veterinary Medicine and Animal Science São Paulo State University (Unesp) Department of Veterinary Surgery and Anesthesiology School of Veterinary Medicine and Animal Science São Paulo State University (Unesp) Department of Veterinary Pathology School of Agricultural and Veterinarian Sciences São Paulo State University (Unesp) Department of Veterinary Clinic School of Veterinary Medicine and Animal Science São Paulo State University (Unesp) Department of Veterinary Surgery and Anesthesiology School of Veterinary Medicine and Animal Science São Paulo State University (Unesp) CNPq: 422139/2018-1 CNPq: 443884/2014-5

Published
2019

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